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    DISC DIFFUSION Method SHIMAA

    Uploaded by

    Maryam Adel
    The document describes the disk diffusion method for antibiotic susceptibility testing. This method involves preparing a bacterial suspension, streaking bacterial cultures on agar plates, applying antibiotic disks to the plates, incubating the plates overnight, and measuring any zones of inhibition around the disks to determine antibiotic susceptibility. Key steps include preparing a 0.5 McFarland standard inoculum, ensuring dried plates before applying disks spaced 1.5-2 cm apart, incubating plates overnight at 30-35°C, and measuring zones of inhibition in millimeters.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd

    DISC DIFFUSION Method SHIMAA

    Uploaded by

    Maryam Adel
    24 views25 pages
    The document describes the disk diffusion method for antibiotic susceptibility testing. This method involves preparing a bacterial suspension, streaking bacterial cultures on agar plates, applying antibiotic disks to the plates, incubating the plates overnight, and measuring any zones of inhibition around the disks to determine antibiotic susceptibility. Key steps include preparing a 0.5 McFarland standard inoculum, ensuring dried plates before applying disks spaced 1.5-2 cm apart, incubating plates overnight at 30-35°C, and measuring zones of inhibition in millimeters.

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    DISC DIFFUSION method SHIMAA

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    The document describes the disk diffusion method for antibiotic susceptibility testing. This method involves preparing a bacterial suspension, streaking bacterial cultures on agar plates, applying antibiotic disks to the plates, incubating the plates overnight, and measuring any zones of inhibition around the disks to determine antibiotic susceptibility. Key steps include preparing a 0.5 McFarland standard inoculum, ensuring dried plates before applying disks spaced 1.5-2 cm apart, incubating plates overnight at 30-35°C, and measuring zones of inhibition in millimeters.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    24 views25 pages

    DISC DIFFUSION Method SHIMAA

    Uploaded by

    Maryam Adel
    The document describes the disk diffusion method for antibiotic susceptibility testing. This method involves preparing a bacterial suspension, streaking bacterial cultures on agar plates, applying antibiotic disks to the plates, incubating the plates overnight, and measuring any zones of inhibition around the disks to determine antibiotic susceptibility. Key steps include preparing a 0.5 McFarland standard inoculum, ensuring dried plates before applying disks spaced 1.5-2 cm apart, incubating plates overnight at 30-35°C, and measuring zones of inhibition in millimeters.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 25

    DIFFUSION METHOD

    DISC DIFFUSION
    (KIRBY_BAUER)
    PRIMARY GOALS

    ANNUAL
    REVENU
    E
    GROWTH
    3
    PRESENTATION TITLE
    INTRODUCTION:
    4
    PRESENTATION TITLE
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    PRESENTATION TITLE
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    PRESENTATION TITLE
    7
    PRESENTATION TITLE
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    PRESENTATION TITLE
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    PRESENTATION TITLE
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    PRESENTATION TITLE
    PRESENTATION TITLE

    DISK DIFFUSION: INOCULUM


    PREPARATION
    • A bacterial suspension with turbidity
    equivalent to 0.5 MacFarland is prepared
    in saline or nutrient broth.
    • Soak a sterile cotton swab in the bacterial
    suspension.
    • Remove excess fluid by pressing the swab
    against the tube wall

    11
    PRESENTATION TITLE

    Disk Diffusion: plate streaking

    • Streak the swab evenly over the surface of the


    Müller-Hinton medium in three directions to
    ensure even distribution of bacteria.
    • With petri dish lid in place, allow 3- 5 minutes
    (no longer than 15 minutes) for agar surface to
    dry
    12
    PRESENTATION TITLE

    DISK DIFFUSION: APPLYING


    DISKS
    • Using sterile thin forceps, place
    antibiotic discs to the surface of
    the agar.
    • Antibiotic discs should be 1.5-2
    cm spaced otherwise inhibition
    zones will overlap.
    • Within 30 minutes of applying
    the discs, the plates are inverted
    and incubated overnight at 30-35°
    C.
    13
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    PRESENTATION TITLE
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    PRESENTATION TITLE
    PRESENTATION TITLE U S E TR A N S M I TTE D LI G H T , RATH E R T H A N R EF L ECT ED L I G H T,
    W H EN MEA SU RI N G ZO N ES

    17
    PRESENTATION TITLE
    M EA SU RI N G T H E ZO N E O F I N H I BI TI O N BY R U L ER O R M ET RI C I N MM I N
    D I A MET ER

    18
    PRESENTATION TITLE

    • Do not use disks beyond their expiration date.


    • Do not store disks in a frost-free freezer.
    • Use FDA cleared products.
    • Use disks with the content specified in CLSI standards.
    • Do not relocate a disk once it has touched the agar
    surface

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    PRESENTATION TITLE
    PRESENTATION TITLE

    Click icon to add picture

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    PRESENTATION TITLE

    Measure the point at which you can see an obvious demarcation


    22
    between growth and no growth. Measure the obvious zone.
    Ignore the swarm even if it covers the zone
    PRESENTATION TITLE

    • Zones with
    trimethoprimsulfamethoxazole
    (and also sulfonamides and
    trimethoprim alone) may be
    difficult to read because this
    agent may not inhibit bacteria
    from multiplying until the
    bacteria have gone through
    several generations of growth.
    • You may see a light haze of
    growth within the zone.
    Measure the zone at the point
    where there is an 80% reduction
    in growth
    23
    ADVANTAGE AND DISADVANTAGES
    PRESENTATION TITLE

    • Convenient and user-


    friendly method

    • It is generally not
    accepted to test slow
    growing bacteria that
    require extended
    incubation such as
    mycobacteria and
    anaerobes.

    • Lack of interpretive
    24 criteria for many organism
    THANK YOU

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