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    Basic Apparatus and Equipment in Microbiology

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    Tom Anthony Tonguia
    This document outlines basic apparatus and equipment used in microbiology laboratories, including incubators for culturing microorganisms under controlled temperature and moisture conditions, petri dishes and test tubes/flasks for culturing microorganisms in solid and liquid media respectively, hot plates for heating and mixing solutions, water baths for controlled heating of liquids, autoclaves for reliable sterilization through high pressure steam, and laminar flow hoods which reduce contamination risk through filtered airflow.

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    Basic Apparatus and Equipment in Microbiology

    Uploaded by

    Tom Anthony Tonguia
    214 views10 pages
    This document outlines basic apparatus and equipment used in microbiology laboratories, including incubators for culturing microorganisms under controlled temperature and moisture conditions, petri dishes and test tubes/flasks for culturing microorganisms in solid and liquid media respectively, hot plates for heating and mixing solutions, water baths for controlled heating of liquids, autoclaves for reliable sterilization through high pressure steam, and laminar flow hoods which reduce contamination risk through filtered airflow.

    Original Title

    BASIC APPARATUS AND EQUIPMENT IN MICROBIOLOGY

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    This document outlines basic apparatus and equipment used in microbiology laboratories, including incubators for culturing microorganisms under controlled temperature and moisture conditions, petri dishes and test tubes/flasks for culturing microorganisms in solid and liquid media respectively, hot plates for heating and mixing solutions, water baths for controlled heating of liquids, autoclaves for reliable sterilization through high pressure steam, and laminar flow hoods which reduce contamination risk through filtered airflow.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    214 views10 pages

    Basic Apparatus and Equipment in Microbiology

    Uploaded by

    Tom Anthony Tonguia
    This document outlines basic apparatus and equipment used in microbiology laboratories, including incubators for culturing microorganisms under controlled temperature and moisture conditions, petri dishes and test tubes/flasks for culturing microorganisms in solid and liquid media respectively, hot plates for heating and mixing solutions, water baths for controlled heating of liquids, autoclaves for reliable sterilization through high pressure steam, and laminar flow hoods which reduce contamination risk through filtered airflow.

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    © All Rights Reserved
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    BASIC APPARATUS AND

    EQUIPMENT IN
    MICROBIOLOGY
    Presented by: Tom Anthony A. Tonguia, RMT
    Objectives

     Identify the different equipment and apparatus used in the bacteriology


    laboratory;
     Describe the functions and uses of each equipment; and
     Illustrate the different materials and apparatus.
    Introduction

     Microbiology utilizes many different laboratory apparatus and equipment.


     Microbiological studies and experiments employ such techniques as sterilization,
    isolation of bacteria, culture media preparation, and biochemical testing among
    others.
    Microbiological Incubator

     Designed for the cultivation of microorganism cultures contained in Petri dishes,


    flasks, or test tubes, or other glassware or disposable plastic ware under
    controlled conditions of temperature, moisture and gas content.
    Petri Dish

     Used to culture microorganisms in solid media


    Test Tubes or Flasks

     Flasks or test tubes are used to culture microorganisms in liquid medium.


     Test tubes are also used for containing aqueous chemical reactions and
    observations such as biochemical tests.
     Flasks such as the Erlenmeyer flask are also used to mix liquids together and may
    be heated as well as to facilitate mixing.
     They are often used in the preparation of culture media.
    Hot Plate

     It is used for general heating and mixing.


     It serves to provide even heating and is often equipped with a magnetic stirring
    function.
     A magnet coated in plastic is placed in the mixture and the stirrer function is
    activated which creates a varying magnetic field causing the magnet to spin
    resulting in the mixing of the fluid being heated.
    Water Steam Bath

     It is used to heat liquids and solutions.


     This is utilized most often when the solution being heated may burn easily or
    there is a need for slow heating.
     The same instrument may also be used in conducting controlled temperature
    experiments that may be required in biochemical testing in place of an incubator.
     This instrument may also be used for sterilization.
    Autoclave

     It is indispensable equipment for sterilization.


     Of all sterilization methods, high pressure steam is most widely used because of
    its efficiency, speed, and reliability.
     This method is suitable for a wide range of media, glass, and metal implements,
    and waste products.
     It provides a safe, reliable, high pressure steam sterilizing environment.
    Laminar Flow Hood

     Simply known as the hood, is a closed chamber devise that reduces the risk of
    bacterial contamination and assures that experiments are prepared in the most
    aseptic manner.
     The hood is designed to prevent contamination to whatever one is handling or
    experimenting on.
     It prevents contamination of cultures as microorganisms are being transferred.
     The laminar flow hood is one among many different types of hoods utilized in
    laboratories.

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