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    Biofilms in QC

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    Alla Suglobova
    Biofilms are complex microbial communities embedded in an extracellular matrix that attach to surfaces. They are composed of 15% microbial cells and 85% matrix such as polysaccharides and proteins. The matrix forms towers and channels that allow for nutrient and waste flow. Biofilms provide survival advantages for microbes against environmental stresses and are found universally, including on industrial surfaces and medical devices.

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    Biofilms in QC

    Uploaded by

    Alla Suglobova
    20 views10 pages
    Biofilms are complex microbial communities embedded in an extracellular matrix that attach to surfaces. They are composed of 15% microbial cells and 85% matrix such as polysaccharides and proteins. The matrix forms towers and channels that allow for nutrient and waste flow. Biofilms provide survival advantages for microbes against environmental stresses and are found universally, including on industrial surfaces and medical devices.

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    Biofilms in pharmaceutical quality control

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    Biofilms are complex microbial communities embedded in an extracellular matrix that attach to surfaces. They are composed of 15% microbial cells and 85% matrix such as polysaccharides and proteins. The matrix forms towers and channels that allow for nutrient and waste flow. Biofilms provide survival advantages for microbes against environmental stresses and are found universally, including on industrial surfaces and medical devices.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    20 views10 pages

    Biofilms in QC

    Uploaded by

    Alla Suglobova
    Biofilms are complex microbial communities embedded in an extracellular matrix that attach to surfaces. They are composed of 15% microbial cells and 85% matrix such as polysaccharides and proteins. The matrix forms towers and channels that allow for nutrient and waste flow. Biofilms provide survival advantages for microbes against environmental stresses and are found universally, including on industrial surfaces and medical devices.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    of 10

    Biofilms in pharmaceutical

    quality control

    Alla Suhlobova
    Biofilms
    Biofilms are complex and three-dimensional microbial sessile
    communities of cells embedded in a matrix of extra polymeric
    substances (EPS) and irreversibly attached to a substrate,
    interface, or to each other.
     Biofilms are universal. Biofilm is found to be a preferred
    way of life for bacteria because it represents a survival
    strategy as a mul-tilayer defense mechanism against
    environmental stresses.
     EPS matrix secreted by the organisms, which helps protect
    and keep the microbial community together, is clearly visible.
    Biofilm Structure
    A native biofilm, which has a microcolony as its basic structural
    unit, contains about 15% microbial cells and 85% EPS, the latter
    composed of polysaccharides, proteins, other polymers, and
    water.

    The EPS matrix forms “towers” and “mushrooms” shapes.


    Within these towers, water channels are formed interspersed
    between the sessile microbial cells where nutrients, oxygen, and
    waste flow through.
    Biofilm Structure

    Scanning electron micrograph depicting a Staphylococcus


    aureus biofilm found on the luminal surface of an indwelling
    catheter.
    Biofilm Structure
     The chemical composition, physical properties,
    hydrophobicity, and solubility of the EPS matrix vary with the
    microorganisms, and age of biofilm, and also within the
    biofilm structure itself.
     Most Gram-negative organisms secrete polysaccharides that
    are neutral or polyanionic, whereas Gram-positive bacteria
    secrete EPS that are quite different chemically and tend to be
    cationic. Biofilms also have aerobic and anaerobic portions.
    Even in a perfectly aerobic environment (e.g., a very thin
    biofilm), the head of the mushroom is anaerobic.
     Often, noncellular materials, such as mineral crystals and
    corrosion particles, are found within the biofilm matrix.
    Biofilms structure

    Conceptual illustration of biofilm structure showing bacterial clusters,


    streamers, and water channels..
    Biofilm formation and quorum session

    Illustration of stages of biofilm formation.


    Biofilms structure

    Biofilm on a Stainless Steel Surface A biofilm is a polysaccharide-encased community of


    microorganisms.
    Biofilms structure

    Architecture of a Biofilm Superimposed time sequence image shows


    a single latex bead moving through a biofilm water channel. The
    large light gray shapes are clusters of bacteria (scale bar=50 mm)
    Biofilm formation and quorum session

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