This document summarizes a study that used computational fluid dynamics (CFD) analysis to evaluate heat transfer performance in a tube with twisted tape inserts using different working fluids. The CFD simulations modeled fluid flow and heat transfer in the tube domain using water as the base fluid and then two types of nanofluids (Al2O3/water and CuO/water) at various volume fractions and inlet velocities. Key results found that the Al2O3/water nanofluid provided the best heat transfer enhancement compared to the other fluids, with up to a 43.7% increase in heat transfer over water. The study demonstrates the effectiveness of nanofluids for improving heat transfer characteristics in pipes.