This document discusses various chromatography techniques including paper chromatography, thin layer chromatography, partition chromatography, adsorption chromatography, ion-exchange chromatography, gel filtration chromatography, affinity chromatography, high performance liquid chromatography, and gas liquid chromatography. The different techniques separate molecules based on properties like size, charge, and affinity. They have various applications in biochemistry, like purifying antibodies using affinity for the antigen.
36. Affinity chromatography- Significance
By using antibodies, antigens can be easily separated. Conversely,
antibodies can be purified by passing through a column containing the
antigen.
Specific Antibody binds to a specific antigen attached to the stationary medium, the
remaining antibodies come out in earlier fractions. Biochemistry of Medics
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