Chromatography
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This document discusses various chromatography techniques including paper chromatography, thin layer chromatography, partition chromatography, adsorption chromatography, ion-exchange chromatography, gel filtration chromatography, affinity chromatography, high performance liquid chromatography, and gas chromatography. The different techniques separate molecules based on properties like size, charge, and affinity. They have various applications in biochemistry like purifying proteins, antibodies, and separating biological compounds.
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Chromatography
- 1. CHROMATOGRAPHY Biochemistry of Medics 1
- 2. Chromatography Biochemistry of Medics 2
- 3. Chromatography Biochemistry of Medics 3
- 4. Chromatography- Principle Biochemistry of Medics 4
- 5. Chromatography- Classification Biochemistry of Medics 5
- 6. Chromatography- Classification(Contd.) Biochemistry of Medics 6
- 7. Partition Chromatography Biochemistry of Medics 7
- 8. Paper chromatography Biochemistry of Medics 8
- 9. Paper chromatography Biochemistry of Medics 9
- 10. Paper chromatography- Procedure Biochemistry of Medics 10
- 11. Paper chromatography- Procedure Biochemistry of Medics 11
- 12. Ascending and Descending Paper Chromatography Biochemistry of Medics 12
- 13. Paper Chromatography -Analysis Biochemistry of Medics 13
- 14. Rƒ value Biochemistry of Medics 14
- 15. Two dimensional chromatography Biochemistry of Medics 15
- 16. Significance of Paper chromatography Biochemistry of Medics 16
- 17. Thin layer chromatography (TLC) Biochemistry of Medics 17
- 18. TLC- Procedure Biochemistry of Medics 18
- 19. TLC -Overview Biochemistry of Medics 19
- 20. Significance of TLC Biochemistry of Medics 20
- 21. Advantages of TLC over paper chromatography Biochemistry of Medics 21
- 22. 2)Adsorption chromatography Biochemistry of Medics 22
- 23. 2)Adsorption chromatography- Elution Biochemistry of Medics 23
- 24. Adsorption chromatography The substance loosely adsorbed to the stationary medium comes in the earlier fraction. Biochemistry of Medics 24
- 25. Ion – Exchange chromatography Biochemistry of Medics 25
- 26. Ion – Exchange chromatography Biochemistry of Medics 26
- 27. Ion – Exchange chromatography- Procedure Biochemistry of Medics 27
- 28. Ion – Exchange chromatography- Procedure (Contd.) Biochemistry of Medics 28
- 29. Types of ion exchanges resins Biochemistry of Medics 29
- 30. Ion – Exchange chromatography- An Overview Biochemistry of Medics 30
- 31. 4) Gel filtration chromatography or Molecular Sieve chromatography Biochemistry of Medics 31
- 32. Gel filtration chromatography or Molecular Sieve chromatography Biochemistry of Medics 32
- 33. Gel filtration chromatography-An Overview Larger particles come out first, while smaller particles come in later fractions Biochemistry of Medics 33
- 34. 5) Affinity chromatography Biochemistry of Medics 34
- 35. Affinity chromatography- Significance Biochemistry of Medics 35
- 36. Affinity chromatography- Significance By using antibodies, antigens can be easily separated. Conversely, antibodies can be purified by passing through a column containing the antigen. Specific Antibody binds to a specific antigen attached to the stationary medium, the remaining antibodies come out in earlier fractions. Biochemistry of Medics 36
- 37. 6) High performance liquid chromatography- Biochemistry of Medics 37
- 38. High performance liquid chromatography- Biochemistry of Medics 38
- 39. High performance liquid chromatography- Significance Biochemistry of Medics 39
- 40. High performance liquid chromatography- Apparatus Biochemistry of Medics 40
- 41. 6) Gas liquid chromatography- Biochemistry of Medics 41
- 42. Gas liquid chromatography- Biochemistry of Medics 42
- 43. Gas liquid chromatography-Apparatus Biochemistry of Medics 43
- 44. Summary Biochemistry of Medics 44