Surface plasmon resonance (SPR) sensors are optical sensors that can detect minute changes in the refractive index near a metal surface. They have various applications in biomedical sensing, environmental monitoring, and more. SPR sensors can be classified as surface plasmon polariton-based or localized surface plasmon resonance-based. Sensitivity, detection limit, and dynamic range are important characteristics. SPR sensing can be performed through angular modulation, wavelength modulation, intensity modulation, or phase/polarization modulation. Diffraction gratings and prism couplers are common methods used to excite surface plasmons. Localized SPR sensors offer advantages like simpler instrumentation but lower sensitivity compared to SPR sensors.
Surface plasmon resonance (SPR) is a technique that uses surface plasmons - collective oscillations of electrons at the interface between a metal and a dielectric - to detect changes in the refractive index near the surface. SPR can be used as a highly sensitive biosensor to detect molecular interactions in real-time without labeling. It has applications in areas like biomolecular interaction analysis, epitope mapping, and evaluating non-specific binding for purposes like bio-compatibility testing and tissue engineering.
Surface Plasmon Resonance,
Surface Plasmons:
Plasmons confined to surface (interface) and interact with light resulting in polarities.
Propagating electron density waves occurring at the interface between metal and dielectric.
This document summarizes research on using bimetallic nanoparticles to enhance surface plasmon resonance. Laser ablation in liquids was used to prepare silver, gold, silver-gold mixture, and silver core/gold shell nanoparticles in aqueous solution. The surface plasmon resonance peaks of the nanoparticles could be tuned from 532 to 546 nm by varying the laser parameters, which changed the nanoparticle size and distribution. Increasing the gold shell ablation time enhanced the intensity of the surface plasmon resonance bands. This research demonstrates that bimetallic nanoparticles allow tunable surface plasmon resonance for applications such as optical communication systems and tunable wavelength filters.
- Surface plasmon polaritons are electromagnetic waves that propagate along the interface between a metal and a dielectric material. They arise from the coupling of incident light to oscillations of surface electrons known as surface plasmons.
- Surface plasmon polaritons can be excited through techniques like prism coupling using either the Otto or Kretschmann configurations, which use evanescent waves to overcome the momentum mismatch between incident light and surface plasmons.
- Applications of surface plasmon resonance include ultrasensitive biosensing, fluorescence imaging, catalysis, and phototherapy due to the ability of surface plasmons to concentrate electromagnetic fields at subwavelength scales.
1. Surface plasmon resonance (SPR) involves the resonant oscillation of conduction electrons stimulated by incident light at a metal-dielectric interface. SPR can be used to detect changes in refractive index within 300 nm of a sensor surface.
2. There are three main configurations for SPR sensors: prism-based, grating-based, and optical waveguide-based. Factors that affect SPR include the type of metal used, the structure and composition of the metal surface, and the properties of the incident light.
3. SPR biosensors can be used for applications in the dairy industry like detection of antibiotics, proteins in milk powder, food-borne pathogens like Listeria monocytogenes and Salmonella.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
This document summarizes research on plasmonics and surface plasmon polaritons (SPPs). It discusses two types of excitations - localized surface plasmon resonance and propagating SPPs. Applications mentioned include spectroscopy, molecular detection, cancer treatment, photonic devices, integrated photonics, and optical data storage. Challenges include losses, thermal effects, and limitations of nanofabrication techniques. The document also reviews using SPPs for applications such as beam collimation, near-field microscopy, solar cells, and metamaterials.
Atomic force microscopy (AFM) uses a sharp tip attached to a flexible cantilever to scan the surface of a sample and map its topography with nanoscale resolution. As the tip is scanned across the surface, interactions between the tip and sample cause the cantilever to deflect, and these deflections are used to construct a 3D image of the surface. AFM can operate in contact, non-contact, or tapping mode and is capable of measuring properties like roughness, elasticity, and adhesion in addition to topography. It provides magnification from 100X to over 100 million X with nanometer scale resolution and does not require complex sample preparation, making AFM a versatile high-resolution imaging tool.
Surface plasmon resonance (SPR) is a technique that uses surface plasmons - collective oscillations of electrons at the interface between a metal and a dielectric - to detect changes in the refractive index near the surface. SPR can be used as a highly sensitive biosensor to detect molecular interactions in real-time without labeling. It has applications in areas like biomolecular interaction analysis, epitope mapping, and evaluating non-specific binding for purposes like bio-compatibility testing and tissue engineering.
Surface Plasmon Resonance,
Surface Plasmons:
Plasmons confined to surface (interface) and interact with light resulting in polarities.
Propagating electron density waves occurring at the interface between metal and dielectric.
This document summarizes research on using bimetallic nanoparticles to enhance surface plasmon resonance. Laser ablation in liquids was used to prepare silver, gold, silver-gold mixture, and silver core/gold shell nanoparticles in aqueous solution. The surface plasmon resonance peaks of the nanoparticles could be tuned from 532 to 546 nm by varying the laser parameters, which changed the nanoparticle size and distribution. Increasing the gold shell ablation time enhanced the intensity of the surface plasmon resonance bands. This research demonstrates that bimetallic nanoparticles allow tunable surface plasmon resonance for applications such as optical communication systems and tunable wavelength filters.
- Surface plasmon polaritons are electromagnetic waves that propagate along the interface between a metal and a dielectric material. They arise from the coupling of incident light to oscillations of surface electrons known as surface plasmons.
- Surface plasmon polaritons can be excited through techniques like prism coupling using either the Otto or Kretschmann configurations, which use evanescent waves to overcome the momentum mismatch between incident light and surface plasmons.
- Applications of surface plasmon resonance include ultrasensitive biosensing, fluorescence imaging, catalysis, and phototherapy due to the ability of surface plasmons to concentrate electromagnetic fields at subwavelength scales.
1. Surface plasmon resonance (SPR) involves the resonant oscillation of conduction electrons stimulated by incident light at a metal-dielectric interface. SPR can be used to detect changes in refractive index within 300 nm of a sensor surface.
2. There are three main configurations for SPR sensors: prism-based, grating-based, and optical waveguide-based. Factors that affect SPR include the type of metal used, the structure and composition of the metal surface, and the properties of the incident light.
3. SPR biosensors can be used for applications in the dairy industry like detection of antibiotics, proteins in milk powder, food-borne pathogens like Listeria monocytogenes and Salmonella.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
This document summarizes research on plasmonics and surface plasmon polaritons (SPPs). It discusses two types of excitations - localized surface plasmon resonance and propagating SPPs. Applications mentioned include spectroscopy, molecular detection, cancer treatment, photonic devices, integrated photonics, and optical data storage. Challenges include losses, thermal effects, and limitations of nanofabrication techniques. The document also reviews using SPPs for applications such as beam collimation, near-field microscopy, solar cells, and metamaterials.
Atomic force microscopy (AFM) uses a sharp tip attached to a flexible cantilever to scan the surface of a sample and map its topography with nanoscale resolution. As the tip is scanned across the surface, interactions between the tip and sample cause the cantilever to deflect, and these deflections are used to construct a 3D image of the surface. AFM can operate in contact, non-contact, or tapping mode and is capable of measuring properties like roughness, elasticity, and adhesion in addition to topography. It provides magnification from 100X to over 100 million X with nanometer scale resolution and does not require complex sample preparation, making AFM a versatile high-resolution imaging tool.
This document discusses nonlinear optics and summarizes key topics covered:
- It describes the difference between linear and nonlinear optics, where linear optics involves weak light that is unchanged and nonlinear optics involves intense light that can induce effects and be manipulated.
- Nonlinear optics allows changing light properties like color and shape, and has applications in telecommunications and creating ultrashort events.
- Phenomena like sum and difference frequency generation are examples of second-order nonlinear optical effects. Phase matching is important for efficient nonlinear optical processes.
- Applications of nonlinear optics include optical phase conjugation, optical parametric oscillators, optical computing, optical switching, and optical data storage.
This document provides an overview of plasmonics and subwavelength plasmonic waveguides and metamaterials. It begins with definitions of key plasmonic concepts like surface plasmon polaritons and localized surface plasmons. It then discusses the scientific background of plasmonics using the Drude model. Specific plasmonic materials like gold, silver, and aluminum are also examined. Different excitation methods for surface plasmon polaritons are outlined, including prism coupling and grating coupling. The document concludes by mentioning some applications and research trends in plasmonics.
The document provides information about scanning tunneling microscopy (STM). It begins by explaining the quantum mechanical principles behind STM, specifically electron tunneling. It then describes the key components of an STM, including the scanning tip, piezoelectric scanner, distance control system, data processing unit, and vibration isolation system. The document discusses the two main imaging modes of STM - constant height mode and constant current mode. It also outlines how STM works by applying a voltage bias between the tip and sample and measuring the tunneling current. The document concludes by discussing advantages and disadvantages of STM as well as sources of artifacts in STM images.
Nonlinear optics involves intense light interacting with matter to change the light's properties. This allows generating new frequencies of light from the input light. Second harmonic generation produces light with twice the frequency by combining two photons. High harmonic generation using intense lasers can generate coherent x-rays. Phase matching is important for high conversion efficiency in nonlinear optical processes. Applications include optical switching, data storage, and generating coherent x-rays for attosecond science.
Atomic force microscopy (AFM) was developed in 1986 as an extension of scanning tunneling microscopy to image non-conductive surfaces. AFM uses a sharp probe at the end of a flexible cantilever to measure the tiny forces between the probe and sample surface. As the probe scans the surface, these interatomic forces cause the cantilever to deflect, and a laser detects these deflections to create a 3D topographic image of the surface with angstrom-scale resolution. AFM provides topographic and force measurements and can image surfaces in open air or liquid with minimal sample preparation. It has applications in fields including solid state physics, molecular biology, and materials science.
Quantum dots are semiconductor nanoparticles that confine electrons and holes in all three dimensions. They are made using different methods like lithography, colloidal synthesis, or epitaxy. Quantum dots have discrete energy levels that depend on their size and shape. They have potential applications in solar cells, LEDs, bioimaging, drug delivery, and anti-counterfeiting due to their tunable light emission properties.
Nanobiosensors are biosensors on the nano-scale that use biological recognition elements connected to nanoscale transducers. They can detect analytes using techniques like optical measurements, electrochemical methods, electrical sensors like field effect transistors, and nanowires. Nanobiosensors have applications in detecting DNA, proteins, cells, and more for uses in healthcare, environmental monitoring, and other areas due to their high sensitivity and selectivity at the nano-scale level.
Interband and intraband electronic transition in quantum nanostructuresGandhimathi Muthuselvam
This document discusses various types of electronic transitions that can occur in quantum nanostructures, including interband transitions, intraband transitions, and excitonic transitions. It explains that interband transitions involve an electron changing energy levels between different bands, like from the valence band to the conduction band, while intraband transitions are within the same band. The document also covers radiative and non-radiative recombination processes that can result from these transitions. Specifically, it describes how radiative recombination involves the emission of a photon, which is important for semiconductor light sources like lasers and LEDs. The properties of different materials, like direct vs. indirect bandgap, also impact which types of transitions are more likely.
The document presents information on the topic of plasmonics. It discusses how surface plasmonics involves the interaction of light with metallic nanostructures. Surface plasmons are electromagnetic waves that propagate along metal-dielectric interfaces. The document reviews several papers focusing on different aspects of plasmonics, including optical metasurfaces, extraordinary optical transmission, quantum plasmonics, amplification and lasing of plasmonic modes, and plasmonic applications in areas such as biosensing and nanophotonics. Plasmonics is presented as an expanding field that provides opportunities for extremely small and fast photonic devices by bridging electronics and photonics.
Photonic crystals are periodic dielectric structures that have a band gap that forbids propagation of a certain frequency range of light. This property enables one to control light with amazing facility and produce effects that are impossible with conventional optics.Photonic crystals can be fabricated for one, two, or three dimensions. One-dimensional photonic crystals can be made of layers deposited or stuck together. Two-dimensional ones can be made by photolithography, or by drilling holes in a suitable substrate. Fabrication methods for three-dimensional ones include drilling under different angles, stacking multiple 2-D layers on top of each other, direct laser writing, or, for example, instigating self-assembly of spheres in a matrix and dissolving the spheres
PLASMONS: A modern form of super particle wavesDHRUVIN PATEL
The document discusses surface plasmons, which are coherent electron oscillations that exist at the interface between two materials like metal and air. Surface plasmon polaritons are electromagnetic waves that travel along such an interface and involve both charge motion in the metal and electromagnetic waves. They have applications in improving solar cell efficiency through increased light absorption and extraction, as well as medical uses like cancer therapy.
This document discusses electrochemical sensors for detecting antibiotic residues in food. It begins with an introduction on the increasing global use of antibiotics and development of antibiotic resistance. It then discusses the working principles of electrochemical sensors and how they can be used to detect antibiotics. Specifically, it describes how electrochemical sensors use recognition elements like enzymes, antibodies, aptamers, and molecularly imprinted polymers to detect antibiotics. It also discusses using different electrode systems and materials like carbon nanotubes, nanoparticles, and graphene to improve detection. The document aims to provide an overview of developing electrochemical sensor techniques for antibiotic residue detection in food.
Scanning Probe microscopy (AFM and STM) head point
AFM: Configuration of AFM
Parts of AFM system and Principle of AFM
Three Modes of AFM
AFM Instrument
Advantage and disadvantage
STM
Schematic Diagram
AFM and STM
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
Scanning tunneling microscopy (STM) is a technique used to image surfaces at the atomic level. It was developed in 1981 by Gerd Binnig and Heinrich Rohrer at IBM, based on the concept of quantum tunneling. The STM works by using a scanning tip, typically made of tungsten, which is brought very close to the sample surface. A bias is applied between the tip and sample, allowing electrons to tunnel through the vacuum gap. The tunneling current depends on factors like the voltage and position of the tip relative to the sample, enabling atomic resolution images to be produced. Piezoelectric materials are crucial for providing precise movement of the tip in the angstrom scale needed. STM opened
The atomic force microscope (AFM) was invented in 1985 by Gerd Binnig and Cristoph Gerber. It uses a sharp tip mounted on a flexible cantilever to scan the topography of a sample at an extremely high resolution down to the atomic level. The AFM works by measuring the interaction forces between the tip and sample surface. It consists of a probe with a sharp tip, a scanner that controls the tip's movement in the x, y, and z directions, and an optical lever system using a laser and photodetector to measure the cantilever's deflection. The AFM can image a variety of samples at the nanoscale and provide 3D topographic information.
This document provides an introduction to the field of nanophotonics. It defines nanophotonics as the science and engineering of light-matter interactions that take place on wavelength and subwavelength scales. Examples of nanophotonics in nature are discussed. The foundations of nanophotonics are explored, including similarities between the propagation of photons and electrons. Computational methods for modeling nanophotonic structures like finite difference time domain are also summarized. The effects of quantum confinement on the optical properties of nanostructures are described.
This document discusses nanosensors, including their definition, types, and applications. It describes four main types of nanosensors: optical nanosensors, bio-nanosensors, chemical nanosensors, and physical nanosensors. Specific examples are given for each type, such as proximity sensors for optical nanosensors. Applications discussed include PEPPLES for intracellular sensing, a twin-action nanosensor that responds to both metal ions and temperature, and a multimodal nanosensor capable of detecting multiple electromagnetic characteristics.
Atomic force microscopy (AFM) works by scanning a probe over a sample surface to build up a topographic map with single-atom level resolution without the need for sample preparation. It was invented in 1986 by Binning and first used a cantilever with a diamond tip. The main components are a microscope stage to move the tip and sample, control electronics, and a computer. A piezoelectric transducer moves the tip while a force transducer senses the force and feedback control maintains a set force. There are different imaging modes including contact, non-contact, and tapping modes that use repulsive or attractive forces between the probe and sample. AFM can image a variety of biological and material science samples with limitations
Xps (x ray photoelectron spectroscopy)Zaahir Salam
The document provides an overview of X-ray photoelectron spectroscopy (XPS) technology. XPS works by irradiating a sample surface with x-rays and measuring the kinetic energy and number of electrons that escape from the top 1-10 nm of the material. This allows one to determine the sample's elemental composition and chemical/electronic states. Key aspects discussed include the use of ultra-high vacuum conditions to prevent surface contamination and allow for accurate analysis. Characteristic XPS spectra are produced that contain peaks corresponding to different elemental binding energies.
This document discusses surface plasmon resonance (SPR), which is an optical technique used to study biomolecular interactions in real-time without labeling. It involves immobilizing a ligand on a gold sensor chip and passing analyte molecules over the surface. Changes in the refractive index from binding cause a change in the resonance angle, measured as response units. The sensorgram plots response over time and can reveal kinetic and affinity data. SPR is widely applied in areas like drug discovery, diagnostics, and basic research for its sensitivity, small sample size, and ability to study complex samples in real-time. While it provides valuable insights, ligand configuration may change upon immobilization and sensitivity can be limited.
This document provides an overview of vibrational spectroscopy, specifically reflection absorption infrared spectroscopy (RAIRS). It discusses how RAIRS works by directing infrared radiation at a sample surface, analyzing the reflected beam to determine absorbed frequencies. RAIRS has excellent energy resolution and can study surface species and reactions under various conditions. It is most sensitive for observing adsorption of molecules with transition dipoles arranged along the surface normal. The document also covers instrumentation, theory, selection rules, examples of RAIRS analysis, and limitations.
This document discusses nonlinear optics and summarizes key topics covered:
- It describes the difference between linear and nonlinear optics, where linear optics involves weak light that is unchanged and nonlinear optics involves intense light that can induce effects and be manipulated.
- Nonlinear optics allows changing light properties like color and shape, and has applications in telecommunications and creating ultrashort events.
- Phenomena like sum and difference frequency generation are examples of second-order nonlinear optical effects. Phase matching is important for efficient nonlinear optical processes.
- Applications of nonlinear optics include optical phase conjugation, optical parametric oscillators, optical computing, optical switching, and optical data storage.
This document provides an overview of plasmonics and subwavelength plasmonic waveguides and metamaterials. It begins with definitions of key plasmonic concepts like surface plasmon polaritons and localized surface plasmons. It then discusses the scientific background of plasmonics using the Drude model. Specific plasmonic materials like gold, silver, and aluminum are also examined. Different excitation methods for surface plasmon polaritons are outlined, including prism coupling and grating coupling. The document concludes by mentioning some applications and research trends in plasmonics.
The document provides information about scanning tunneling microscopy (STM). It begins by explaining the quantum mechanical principles behind STM, specifically electron tunneling. It then describes the key components of an STM, including the scanning tip, piezoelectric scanner, distance control system, data processing unit, and vibration isolation system. The document discusses the two main imaging modes of STM - constant height mode and constant current mode. It also outlines how STM works by applying a voltage bias between the tip and sample and measuring the tunneling current. The document concludes by discussing advantages and disadvantages of STM as well as sources of artifacts in STM images.
Nonlinear optics involves intense light interacting with matter to change the light's properties. This allows generating new frequencies of light from the input light. Second harmonic generation produces light with twice the frequency by combining two photons. High harmonic generation using intense lasers can generate coherent x-rays. Phase matching is important for high conversion efficiency in nonlinear optical processes. Applications include optical switching, data storage, and generating coherent x-rays for attosecond science.
Atomic force microscopy (AFM) was developed in 1986 as an extension of scanning tunneling microscopy to image non-conductive surfaces. AFM uses a sharp probe at the end of a flexible cantilever to measure the tiny forces between the probe and sample surface. As the probe scans the surface, these interatomic forces cause the cantilever to deflect, and a laser detects these deflections to create a 3D topographic image of the surface with angstrom-scale resolution. AFM provides topographic and force measurements and can image surfaces in open air or liquid with minimal sample preparation. It has applications in fields including solid state physics, molecular biology, and materials science.
Quantum dots are semiconductor nanoparticles that confine electrons and holes in all three dimensions. They are made using different methods like lithography, colloidal synthesis, or epitaxy. Quantum dots have discrete energy levels that depend on their size and shape. They have potential applications in solar cells, LEDs, bioimaging, drug delivery, and anti-counterfeiting due to their tunable light emission properties.
Nanobiosensors are biosensors on the nano-scale that use biological recognition elements connected to nanoscale transducers. They can detect analytes using techniques like optical measurements, electrochemical methods, electrical sensors like field effect transistors, and nanowires. Nanobiosensors have applications in detecting DNA, proteins, cells, and more for uses in healthcare, environmental monitoring, and other areas due to their high sensitivity and selectivity at the nano-scale level.
Interband and intraband electronic transition in quantum nanostructuresGandhimathi Muthuselvam
This document discusses various types of electronic transitions that can occur in quantum nanostructures, including interband transitions, intraband transitions, and excitonic transitions. It explains that interband transitions involve an electron changing energy levels between different bands, like from the valence band to the conduction band, while intraband transitions are within the same band. The document also covers radiative and non-radiative recombination processes that can result from these transitions. Specifically, it describes how radiative recombination involves the emission of a photon, which is important for semiconductor light sources like lasers and LEDs. The properties of different materials, like direct vs. indirect bandgap, also impact which types of transitions are more likely.
The document presents information on the topic of plasmonics. It discusses how surface plasmonics involves the interaction of light with metallic nanostructures. Surface plasmons are electromagnetic waves that propagate along metal-dielectric interfaces. The document reviews several papers focusing on different aspects of plasmonics, including optical metasurfaces, extraordinary optical transmission, quantum plasmonics, amplification and lasing of plasmonic modes, and plasmonic applications in areas such as biosensing and nanophotonics. Plasmonics is presented as an expanding field that provides opportunities for extremely small and fast photonic devices by bridging electronics and photonics.
Photonic crystals are periodic dielectric structures that have a band gap that forbids propagation of a certain frequency range of light. This property enables one to control light with amazing facility and produce effects that are impossible with conventional optics.Photonic crystals can be fabricated for one, two, or three dimensions. One-dimensional photonic crystals can be made of layers deposited or stuck together. Two-dimensional ones can be made by photolithography, or by drilling holes in a suitable substrate. Fabrication methods for three-dimensional ones include drilling under different angles, stacking multiple 2-D layers on top of each other, direct laser writing, or, for example, instigating self-assembly of spheres in a matrix and dissolving the spheres
PLASMONS: A modern form of super particle wavesDHRUVIN PATEL
The document discusses surface plasmons, which are coherent electron oscillations that exist at the interface between two materials like metal and air. Surface plasmon polaritons are electromagnetic waves that travel along such an interface and involve both charge motion in the metal and electromagnetic waves. They have applications in improving solar cell efficiency through increased light absorption and extraction, as well as medical uses like cancer therapy.
This document discusses electrochemical sensors for detecting antibiotic residues in food. It begins with an introduction on the increasing global use of antibiotics and development of antibiotic resistance. It then discusses the working principles of electrochemical sensors and how they can be used to detect antibiotics. Specifically, it describes how electrochemical sensors use recognition elements like enzymes, antibodies, aptamers, and molecularly imprinted polymers to detect antibiotics. It also discusses using different electrode systems and materials like carbon nanotubes, nanoparticles, and graphene to improve detection. The document aims to provide an overview of developing electrochemical sensor techniques for antibiotic residue detection in food.
Scanning Probe microscopy (AFM and STM) head point
AFM: Configuration of AFM
Parts of AFM system and Principle of AFM
Three Modes of AFM
AFM Instrument
Advantage and disadvantage
STM
Schematic Diagram
AFM and STM
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
Scanning tunneling microscopy (STM) is a technique used to image surfaces at the atomic level. It was developed in 1981 by Gerd Binnig and Heinrich Rohrer at IBM, based on the concept of quantum tunneling. The STM works by using a scanning tip, typically made of tungsten, which is brought very close to the sample surface. A bias is applied between the tip and sample, allowing electrons to tunnel through the vacuum gap. The tunneling current depends on factors like the voltage and position of the tip relative to the sample, enabling atomic resolution images to be produced. Piezoelectric materials are crucial for providing precise movement of the tip in the angstrom scale needed. STM opened
The atomic force microscope (AFM) was invented in 1985 by Gerd Binnig and Cristoph Gerber. It uses a sharp tip mounted on a flexible cantilever to scan the topography of a sample at an extremely high resolution down to the atomic level. The AFM works by measuring the interaction forces between the tip and sample surface. It consists of a probe with a sharp tip, a scanner that controls the tip's movement in the x, y, and z directions, and an optical lever system using a laser and photodetector to measure the cantilever's deflection. The AFM can image a variety of samples at the nanoscale and provide 3D topographic information.
This document provides an introduction to the field of nanophotonics. It defines nanophotonics as the science and engineering of light-matter interactions that take place on wavelength and subwavelength scales. Examples of nanophotonics in nature are discussed. The foundations of nanophotonics are explored, including similarities between the propagation of photons and electrons. Computational methods for modeling nanophotonic structures like finite difference time domain are also summarized. The effects of quantum confinement on the optical properties of nanostructures are described.
This document discusses nanosensors, including their definition, types, and applications. It describes four main types of nanosensors: optical nanosensors, bio-nanosensors, chemical nanosensors, and physical nanosensors. Specific examples are given for each type, such as proximity sensors for optical nanosensors. Applications discussed include PEPPLES for intracellular sensing, a twin-action nanosensor that responds to both metal ions and temperature, and a multimodal nanosensor capable of detecting multiple electromagnetic characteristics.
Atomic force microscopy (AFM) works by scanning a probe over a sample surface to build up a topographic map with single-atom level resolution without the need for sample preparation. It was invented in 1986 by Binning and first used a cantilever with a diamond tip. The main components are a microscope stage to move the tip and sample, control electronics, and a computer. A piezoelectric transducer moves the tip while a force transducer senses the force and feedback control maintains a set force. There are different imaging modes including contact, non-contact, and tapping modes that use repulsive or attractive forces between the probe and sample. AFM can image a variety of biological and material science samples with limitations
Xps (x ray photoelectron spectroscopy)Zaahir Salam
The document provides an overview of X-ray photoelectron spectroscopy (XPS) technology. XPS works by irradiating a sample surface with x-rays and measuring the kinetic energy and number of electrons that escape from the top 1-10 nm of the material. This allows one to determine the sample's elemental composition and chemical/electronic states. Key aspects discussed include the use of ultra-high vacuum conditions to prevent surface contamination and allow for accurate analysis. Characteristic XPS spectra are produced that contain peaks corresponding to different elemental binding energies.
This document discusses surface plasmon resonance (SPR), which is an optical technique used to study biomolecular interactions in real-time without labeling. It involves immobilizing a ligand on a gold sensor chip and passing analyte molecules over the surface. Changes in the refractive index from binding cause a change in the resonance angle, measured as response units. The sensorgram plots response over time and can reveal kinetic and affinity data. SPR is widely applied in areas like drug discovery, diagnostics, and basic research for its sensitivity, small sample size, and ability to study complex samples in real-time. While it provides valuable insights, ligand configuration may change upon immobilization and sensitivity can be limited.
This document provides an overview of vibrational spectroscopy, specifically reflection absorption infrared spectroscopy (RAIRS). It discusses how RAIRS works by directing infrared radiation at a sample surface, analyzing the reflected beam to determine absorbed frequencies. RAIRS has excellent energy resolution and can study surface species and reactions under various conditions. It is most sensitive for observing adsorption of molecules with transition dipoles arranged along the surface normal. The document also covers instrumentation, theory, selection rules, examples of RAIRS analysis, and limitations.
This document discusses plasmonic chain waveguides. Plasmonic chain waveguides use linear chains of plasmonic nanoparticles to concentrate optical beams below the diffraction limit and guide electromagnetic energy. Each nanoparticle acts as a dipole that interacts with the nearest neighboring nanoparticles. This coupling supports localized surface plasmons and guided wave propagation along the chain. Both the particle size and distance between particles impact the coupling strength and guided modes. Plasmonic chain waveguides have applications in nanophotonics due to their ability to squeeze optical signals into subwavelength confinement.
NSOM/SNOM is a scanning probe microscopy technique that can achieve higher resolution than far-field optical microscopy, around 50 nm. It works by exploiting evanescent waves from a sample that are detected using a probe placed within the near-field zone. PINEM is a related technique that uses ultrafast electron pulses synchronized with optical pulses to map photon-electron interactions and image plasmonic fields with high spatiotemporal resolution. Both techniques allow studying nanoscale optical and material properties with applications in nanotechnology, biophysics, and materials science.
This document provides an overview of magnetic resonance techniques for non-destructive testing, specifically nuclear magnetic resonance (NMR) spectroscopy and magnetic resonance imaging (MRI). It discusses the basic principles of how NMR and MRI work, including using magnetic fields and radio waves to detect atomic nuclei like hydrogen protons. Applications mentioned include material characterization, medical imaging, and purity analysis. The instrumentation for both techniques is also described.
This document discusses plasmonic biosensors and their applications. It begins with an abstract stating that plasmonic nanostructures have been used for biosensing applications due to their sensitivity to refractive index changes. It then provides sections on biosensors, plasmonics, plasmonic biosensors, localized surface plasmon resonance sensing, and propagating surface plasmon resonance sensing. Specific applications discussed include the detection of human liver tissues, human blood groups, and hemoglobin concentration in human blood using plasmonic biosensors. The document concludes that the incorporation of biosensors with plasmonics is an important development that could revolutionize medical applications.
Explaining all the difficult concepts with precise and accurate points, 3D models, animations and smart art graphics.
Principle
The NMR phenomenon
Theory
Precessional frequency (ν)
Chemical shift
Spin-spin interactions
Interpretation of NMR
Chemical shift (δ)
Multiplicity of the signal
Coupling constant
Instrumentation
Fourier NMR
Continuous wave NMR
Applications
Identification testing
Assay of drugs
Instrumental Analysis: Spectrophotometric techniques can be used to analyze light-matter interactions. Key components of a spectrophotometer include light sources, monochromators to filter wavelengths, and detectors. Monochromators use diffraction gratings or prisms to separate wavelengths. Detectors like photomultipliers and photodiode arrays convert light signals into electrical signals. Spectrophotometers can be single or double beam, with double beam reducing errors. Fluorescence spectroscopy analyzes emission from electronically excited molecule, providing information about electronic structure.
Surface Plasmon Resonance (SPR) and its ApplicationDr. Barkha Gupta
DR. BARKHA GUPTA
ASSISTANT PROFESSOR (VETERINARY BIOCHEMISTRY)
DEPARTMENT OF VETERINARY PHYSIOLOGY AND BIOCHEMISTRY
POST GRADUATE INSTITUTE OF VETERINARY EDUCATION AND RESEARCH (PGIVER), JAIPUR RAJASTHAN UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES (RAJUVAS), BIKANER
YouTube Channel: Barkha’s Vet Sphere
Photoacoustic spectroscopy is a technique that detects the acoustic waves generated through the absorption of modulated electromagnetic radiation in a sample. It can be used to measure the absorption spectrum of both gases and condensed matter. The absorbed radiation is converted to heat, causing temperature and pressure fluctuations that can be detected by a microphone or piezoelectric transducer. This allows for highly sensitive detection of small absorbers. While it can analyze all phases of matter, photoacoustic spectroscopy has limitations for non-gaseous samples and requires the analyte to absorb the laser light used. It has applications in areas like trace gas analysis, textile dyes, and biomedical samples.
Surface plasmon resonance (SPR) is a phenomenon that occurs when light strikes a metal surface such as gold or silver under specific conditions. This causes surface plasmons to be generated at the metal-dielectric interface, which reduces the intensity of reflected light. SPR is highly sensitive to changes in the refractive index near the metal surface. This property allows SPR to detect the binding of molecules to the metal surface in real-time. Traditionally, SPR is used in biosensor instruments to study biomolecular interactions like protein-ligand binding. However, these instruments have limitations such as high cost and low throughput. The document discusses using SPR with colloidal gold particles as an alternative approach.
The document discusses the history and applications of Raman spectroscopy. It describes how Raman spectroscopy was discovered in 1928 by Sir C.V. Raman using sunlight and optical filters. Raman won the Nobel Prize in 1930 for this discovery. Raman spectroscopy provides information on a sample's chemical composition and molecular structure by analyzing the inelastic scattering of monochromatic light, usually from a laser. It is used to study vibrational, rotational, and other low-frequency modes in a system.
The document discusses Fourier transform infrared spectroscopy (FTIR). It begins by explaining the basic principles of FTIR including how a Fourier transform is used to convert infrared absorption data into a spectrum. It then describes key components of an FTIR instrument and how it works. The document outlines advantages such as high resolution and speed of analysis. Applications including structure determination and identification of organic compounds are also mentioned.
The document provides an overview of Fourier transform infrared spectroscopy (FTIR). It discusses the theory, instrumentation, advantages, and applications of FTIR. The key components of an FTIR instrument are an infrared radiation source, Michelson interferometer, and detector. The interferometer encodes the infrared spectrum into an interferogram that is then decoded via Fourier transform to provide the spectral information. Common detectors include deuterated triglycine sulfate and mercury cadmium telluride. Advantages of FTIR include its simple design, elimination of stray light, and ability to analyze a wide range of organic and inorganic compounds.
The document compares different types of photodetectors. It discusses how photodetectors work by converting light into an electrical signal through generating electron-hole pairs. It classifies photodetectors as either semiconductor-based, which generate electron-hole pairs when exposed to light, or photoemissive, which use the photoelectric effect. Common semiconductor photodetectors include photodiodes, phototransistors, and photoresistors. The document also covers important properties, materials, and operating mechanisms of various photodetector types.
The document discusses scanning probe microscopy (SPM) techniques. It defines local density of states (LDOS) and artifacts. It then discusses the motivation for surface research in electrical engineering due to modern devices' dominance of surface properties. It provides overviews of SPM, atomic force microscopy (AFM), and SPM software. Modes of AFM including contact, friction, tapping, and phase are summarized.
The document compares different types of photodetectors. It begins by defining a photodetector as a device that converts light into an electrical signal through processes like the photovoltaic effect or photoconductivity. It then classifies photodetectors as either semiconductor-based, including photodiodes, or photoemissive, including photomultipliers. The document goes on to provide more details on various photodetector types, their operating principles, important properties, and materials used. It focuses in depth on semiconductor photodetectors like photoresistors, PN diodes, and PIN diodes.
The document compares different types of photodetectors. It begins by defining a photodetector as a device that converts light into an electrical signal through either voltage or current. Photodetectors are then classified as either semiconductor-based, including photovoltaic, photoconductive, and PN junction devices, or photoemissive, which use the photoelectric effect. The document goes on to provide more details on specific photodetector types like photodiodes, phototransistors, and photomultipliers. It also discusses important properties of photodetectors such as sensitivity, response time, and active area.
Theory of NMR, nuclear magnetic resonance, instrumentation, solvents, chemical shift, photon NMR, spin coupling, coupling constant and applications.
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
The document summarizes different methods for synthesizing cadmium sulfide (CdS) nanoparticles. It describes an aqueous precipitation method using cadmium nitrate and sodium sulfide precursors that produces yellow CdS precipitate. It also outlines a sol-gel method using cadmium acetate, diaminobenzene, and thioacetamide precursors that generates a CdS sol and gel. Additionally, it mentions a hydrothermal method using cadmium nitrate, thiourea, and hexamethylenetetramine precursors under high temperature and pressure that can control CdS morphology. The document provides an overview of various preparation techniques for CdS nanoparticles.
This document discusses various approaches for synthesizing nanomaterials, dividing them into top-down and bottom-up categories. Top-down approaches begin with bulk materials and make them smaller, such as through mechanical milling, lithography, sputtering, laser ablation, and electrospinning. Bottom-up approaches build up nanomaterials from molecular components. Common top-down techniques include mechanical milling of materials down to the nanoscale, electrospinning to produce nanofibers, and lithography which uses focused beams of light or electrons to construct nanostructures.
This document provides information on preparing thin films using the Successive Ionic Layer Adsorption and Reaction (SILAR) method. It discusses what thin films are, common thin film deposition techniques like physical vapor deposition and chemical vapor deposition, and the SILAR method specifically. SILAR involves alternating immersion of a substrate in cationic and anionic precursor solutions to deposit materials like cadmium sulfide in a layer-by-layer process. Parameters like concentration, pH, temperature, and deposition time must be optimized to produce adherent thin films. The document also outlines some applications of SILAR-deposited cadmium sulfide thin films and factors that influence thin film characteristics.
Mie theory describes the scattering of electromagnetic radiation by a spherical particle. It provides an exact solution to Maxwell's equations for the scattering of a plane electromagnetic wave by a homogeneous sphere. Gustav Mie provided the mathematical description for the spectral dependence of scattering by a spherical nanoparticle. Mie theory can be used to calculate the absorption and scattering cross sections of nanoparticles and provides the basis for measuring particle size through light scattering. It is valid for particles ranging from much smaller to larger than the wavelength of light.
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive functioning. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
Plasmonic resonance response of metal/dielectric (core/shell) systemsGandhimathi Muthuselvam
This document discusses plasmonic resonance in metal/dielectric core/shell nanostructures. It explains that core-shell structures can precisely control size, shape, composition and orientation to combine different functionalities. The shell acts as a protective barrier for the core while also suppressing surface traps and enhancing fluorescence. Core-shell structures allow tuning of plasmon resonance over a wide wavelength range. Different types of core-shell structures, like metal-dielectric or dielectric-metal, support different plasmon modes depending on shell thickness. Local field enhancement occurs around these structures. Plasmonic voids and hollow structures are also discussed, and how they support hybridized plasmon modes through interactions between constituent parts. Tuning of optical properties in core
This document discusses surface enhanced Raman spectroscopy (SERS) and the mechanisms that lead to signal enhancement. It explains that SERS combines Raman spectroscopy with localized surface plasmon resonance on metallic nanostructures to amplify the weak Raman signal from molecules up to 1011 times. This electromagnetic enhancement is due to the localized electric fields that excite incident photons and enhance molecular emission. Hotspots between nanoparticle gaps produce particularly large field enhancements. The document outlines excitation rate enhancement, emission rate enhancement, and overall SERS enhancement factor calculations.
A crystallite is the smallest unit of a crystal structure and forms a single diffraction pattern, while a grain can contain multiple crystallites of the same phase. Particle size refers to a single crystal or agglomeration of crystals, and is always larger than crystallite size due to multiple crystallites or grain boundaries within particles. Crystallite size is less than or equal to grain size, which is less than or equal to particle size.
This document discusses the Drude model for explaining the optical and electric properties of metals using a free electron gas model. It describes how the Drude model relates the dielectric constant of metals to oscillations of free electrons in response to an applied electromagnetic field. It defines key terms like plasma frequency, damping frequency, and presents equations for the real and imaginary components of the dielectric function and how they describe polarization and energy dissipation in metals. Graphs are shown depicting how the real part of permittivity is negative at lower frequencies but becomes zero near the plasma frequency.
This document provides an overview of nonlinear optics and second harmonic generation. It begins with an introduction to lasers and their components. It then discusses symmetry operations in crystals and how centrosymmetric and noncentrosymmetric materials affect nonlinear polarization. Maxwell's equations are presented for linear media. The document introduces nonlinear optics and lists various nonlinear optical effects such as second harmonic generation. It derives the wave equation for nonlinear media and shows how second harmonic generation leads to frequency doubling. Examples of nonlinear crystals used for second harmonic generation are also provided.
Hospital pharmacy and it's organization (1).pdfShwetaGawande8
The document discuss about the hospital pharmacy and it's organization ,Definition of Hospital pharmacy
,Functions of Hospital pharmacy
,Objectives of Hospital pharmacy
Location and layout of Hospital pharmacy
,Personnel and floor space requirements,
Responsibilities and functions of Hospital pharmacist
How to Create User Notification in Odoo 17Celine George
This slide will represent how to create user notification in Odoo 17. Odoo allows us to create and send custom notifications on some events or actions. We have different types of notification such as sticky notification, rainbow man effect, alert and raise exception warning or validation.
Michael Stevenson EHF Slides June 28th 2024 Shared.pptxEduSkills OECD
Michael Stevenson presents at the webinar 'Will AI in education help students live fulfilling lives?' on 28 June 2024 - https://oecdedutoday.com/oecd-education-webinars/
Get Success with the Latest UiPath UIPATH-ADPV1 Exam Dumps (V11.02) 2024yarusun
Are you worried about your preparation for the UiPath Power Platform Functional Consultant Certification Exam? You can come to DumpsBase to download the latest UiPath UIPATH-ADPV1 exam dumps (V11.02) to evaluate your preparation for the UIPATH-ADPV1 exam with the PDF format and testing engine software. The latest UiPath UIPATH-ADPV1 exam questions and answers go over every subject on the exam so you can easily understand them. You won't need to worry about passing the UIPATH-ADPV1 exam if you master all of these UiPath UIPATH-ADPV1 dumps (V11.02) of DumpsBase. #UIPATH-ADPV1 Dumps #UIPATH-ADPV1 #UIPATH-ADPV1 Exam Dumps
Storytelling for Technical Talks: Building Influence with StakeholdersMattVassar1
Why is that when we present facts alone, we can be met with resistance? Is there another way to influence important stakeholders when it matters most? We discuss how storytelling in technical talks, when done right, can make your ideas more memorable and influential.
Brand Guideline of Bashundhara A4 Paper - 2024khabri85
It outlines the basic identity elements such as symbol, logotype, colors, and typefaces. It provides examples of applying the identity to materials like letterhead, business cards, reports, folders, and websites.
How to stay relevant as a cyber professional: Skills, trends and career paths...Infosec
View the webinar here: https://www.infosecinstitute.com/webinar/stay-relevant-cyber-professional/
As a cybersecurity professional, you need to constantly learn, but what new skills are employers asking for — both now and in the coming years? Join this webinar to learn how to position your career to stay ahead of the latest technology trends, from AI to cloud security to the latest security controls. Then, start future-proofing your career for long-term success.
Join this webinar to learn:
- How the market for cybersecurity professionals is evolving
- Strategies to pivot your skillset and get ahead of the curve
- Top skills to stay relevant in the coming years
- Plus, career questions from live attendees
Artificial Intelligence (AI) has revolutionized the creation of images and videos, enabling the generation of highly realistic and imaginative visual content. Utilizing advanced techniques like Generative Adversarial Networks (GANs) and neural style transfer, AI can transform simple sketches into detailed artwork or blend various styles into unique visual masterpieces. GANs, in particular, function by pitting two neural networks against each other, resulting in the production of remarkably lifelike images. AI's ability to analyze and learn from vast datasets allows it to create visuals that not only mimic human creativity but also push the boundaries of artistic expression, making it a powerful tool in digital media and entertainment industries.
2. Sensors -convert one form energy into electrical energy
Optical sensors-convert light energy into electrical energy
Surface plasmon resonance (SPR) sensor - an optical sensor fabricated based on photonic excitation
Introduction to surface plasmon resonance sensor
Classification
▪ Surface Plasmon Polariton (SPP) based sensor
▪ Localized surface plasmon resonance (LSPR) based
sensors
Plasmonic sensors are fabricated using
▪ nanoparticles
▪ nanopatterned gratings
▪ Prism couplers
▪ Metal/Dielectric waveguide
Characteristics of sensors
▪ Sensitivity
▪ Detection limit
▪ Dynamic range performance
SPR sensor applications
▪ Biomedical
▪ Food science
▪ Environmental monitoring
▪ Toxic or chemical compound
detection
▪ Pharmacy and industry
▪ Medical diagnostics
SPR sensor is vey sensitive to variation in the refractive index of the medium located next to the metallic film
3. ▪ The incident light is directly coupled with SPs (tightly
confined optical field)
▪ Change in the refractive index of the analyte produces a
variation in the propagation constant of the surface plasmon
▪ It means a modification in one of the characteristics of the
optical wave interacting with the surface plasmon
▪ Binding between the analyte and the recognition molecule
caused changes in the refractive index of the dielectric and is
monitored as a shift in the resonance wavelength of the light
A strong EM field oscillation at the interface of metal/dielectric media with
p-polarized incident light resulting in a dark band profile in the light
reflectivity at a specific wavelength(res) and incident angle(I).
SPR Sensor Configuration Surface plasmon Polariton
SPR condition is sensitive to the environment variations and that can be utilized as sensors
Principle
Prism coupler-based SPR sensor
Prism coupler employing the attenuated total reflection method in
Kretschmann geometry is the widely used method in SPR biosensors
applications
4. At Resonance z SPPk k=
2
0 0 2
sin mr a
p
mr a
n
k n k
n
=
+
The expression for the sensitivity is obtained by
differentiating resonant condition equation with respect to
, , I, and na
SPR sensor with
▪ Angular Modulation
▪ Wave length Modulation
▪ Intensity Modulation
▪ Phase or polarization modulation
0 sinz pk k n =Incident light
m mr =
2
d an =where
2
0 0 2
sin mr a
z p
mr a
n
k k n k
n
= =
+
𝑘0 − 𝐹𝑟𝑒𝑒 𝑠𝑝𝑎𝑐𝑒 𝑤𝑎𝑣𝑒 𝑛𝑢𝑚𝑏𝑒𝑟
𝜀 𝑚𝑟 − 𝑅𝑒𝑎𝑙 𝑝𝑎𝑟𝑡 𝑜𝑓 𝑑𝑖𝑒𝑙𝑒𝑐𝑡𝑟𝑖𝑐 𝑐𝑜𝑛𝑠𝑡𝑎𝑛𝑡 𝑜𝑓 𝑚𝑒𝑡𝑎𝑙𝑠
𝑛 𝑝 − 𝑟𝑒𝑓𝑟𝑎𝑐𝑡𝑖𝑣𝑒 index of prism
𝑛 𝑎 − 𝑅𝑒𝑓𝑟𝑎𝑐𝑡𝑖𝑣𝑒 𝑖𝑛𝑑𝑒𝑥 𝑜𝑓 𝑎𝑛𝑎𝑙𝑦𝑡𝑒
Propagation
constant
The excitation of surface plasmons in the SPR sensor results in a change in one
of the characteristics of the light wave. Based on which characteristics of the
light wave is interacting with surface plasmon is measured and used as a sensor
output.
P
a
S
n
=I
a
I
S
n
=𝑆 𝜃 =
𝛿𝜃
𝛿𝑛 𝑎
𝑆𝜆 =
𝛿𝜆 𝑟𝑒𝑠
𝛿𝑛 𝑎
2 m d
SPP
m d
k
=
+
SPP
2
2
sin mr a
p
mr a
n
n
n
=
+
Resonance condition
Classification
Angular, Wavelength, Intensity and phase sensitivity
5. ▪ A monochromatic light wave is employed
to excite the surface plasmon
▪ The excited surface plasmon is observed at
multiple angles of incident light
▪ The strength of coupling between the
incident wave and the surface plasmon
depends upon the angles of incident light
▪ Angle of incidence yielding the strongest
coupling is measured and used as a sensor
output
▪ The sensor output is calibrated to refractive
index
deg
a
S
n RIU
= =
Angular sensitivity
2 deg
10S
RIU
=
- represents the change of resonance angle
-change in the refractive index
𝛿𝜃
𝛿𝑛 𝑎
At constant wavelength
The angle yielding the minimum light intensity on the SPR curve is
denoted as the resonance angle
Addition of diffractive grating and
temperature and noise stabilization are
the ways to increase angular
sensitivity
2 2 2 2 2
( ) ( )
mr mr
a mr a mr a p a p
S
n n n n n n
−
= =
+ − −
Angular modulation
6. ▪ Surface plasmon is excited by a collimated light wave containing multiple wavelengths.
▪ Angle at which the light wave is incident onto the metal film is kept constant.
▪ Coupling strength between the incident wave and SP is observed at multiple wavelengths and the wavelength yielding the strongest
coupling is measured and used as a sensor output
▪ Resonance wavelength is known to shift to the longer wavelength (red shift) as the refractive index at the sensor/dielectric medium is
increased
▪ wavelength Modulation based SPR sensors using prism couplers provide much better sensitivity than their grating-based counterparts
▪ Usage of Furie spectrometers, and multi-channel sensing help to improve sensitivity
3 4
10 10
nm
S
RIU
= −
The wavelength sensitivity of the SPR sensor is defined as the ratio between the
resonance wavelength shift to the variation of the refractive index of the surrounding
medium
Wavelength modulation
where Sλ is the SPR sensor sensitivity
is the shift in the SPR resonance wavelength
is the change in the refractive index
𝛿𝜆 𝑟𝑒𝑠
𝛿𝑛 𝑎
Wavelength sensitivity
2
3
2
( )
2
res mr
pa mr aa
mr a mr
p
S
nn d nn
n
n
= =
+ +
7. 𝛿𝑛 𝑎 = 𝑛2 − 𝑛1
▪ Excitation by single incidence angle and wavelength by changing the intensity of light
▪ P-polarized wave incident light is used and they are very sensitive to any intensity fluctuations of the light source
▪ Light source must be of high quality and stability
▪ Intensity is spatially modulated due to the excitation of surface plasmons and the changes are simultaneously measured in sensing
channel by means of a spatially sensitive detector such as two-dimensional charge coupled device
▪ Sensor output is defined as the difference of these two reflected intensities which is proportional to the reflectance
3 4
1
%
10 10S
RIU
= −
I
a
I
S
n
=
Intensity modulation
The detection of small refractive index changes over a
relatively large volume is successful on sensors based on an
intensity modulation scheme down to a sensitivity of 10-6 RIU
Two light sources with different wavelength help to improve
the sensitivity with intensity modulation
Typical sensitivity- 15000%
𝑅𝐼𝑈
8. ▪ Surface plasmon excitation by shift in phase of the light wave at a incidence angle and wavelength
▪ Explicitly used for the coherent monochromatic light source in SPR instrumentation
▪ It needs phase shift equipment such as a lock in amplifier
where ∆ϕ is the differential phase changes corresponding to ∆n
The phase sensitivity which is defined as
𝛿𝑛 𝑎 = 𝑛2 − 𝑛1
Phase or polarization Modulation
P
a
S
n
=
Other than sensitivity the figure of merit (FOM) is another important parameter to characterize sensor performance
FWHM contains information on light absorption by the binding molecules
𝐹𝑂𝑀 =
𝑆
𝐹𝑊𝐻𝑀
Where S denotes Sensitivity
9. LSPR sensor SPR sensor
Resonance conditions are simpler The energy and momentum matching
conditions should be satisfied
Small size of plasma field (20-40nm)
Marginal bulk effect
Larger plasma field (200-1000nm)
Large Bulk effect
complexity resides in the surface of the
chip
complexity resides in the
instrumentation set up to excite SPR
and read it accurately.
Temperature independent More sensitive to thermal variation
Instrumentally simple Instrumentally complex
Localized surface plasmon resonance (LSPR) sensors
▪ A label-free and powerful surface sensing platform
with higher sensitivity, simple fabrication and
measurement equipment
▪ The extreme chemical sensitivity of metal
nanoparticles to minute changes in the local dielectric
environment, is revealed as a discrete change to their
optical response due to surface adsorption
▪ In LSPR sensor, light passes through the sample
solution are affected by absorption or scattering of the
sample
▪ Requires a simple optical configuration without a
prism
▪ Cost-effective and suitable for miniaturization
10. Analyte
Metal grating
Reflected light
P-polarized
Incident light
Grating period
SPR sensors using diffraction gratings
Incident light 2
sinz ak n
=
Diffracted wave vector
2 2
sinzm ak n m
= +
At resonance
SPP zmk k=
2 2 2
sin m d
a
m d
n m
+ =
+
After Simplification
sin m d
a
m d
n m
+ =
+
At resonance condition
2
2
sin mr a
a
mr a
n
n m
n
+ =
+
2
22
3
3
2 22
a mr
mr amr a
a mra
mr mr a
nm
na nn
nmn
n
+
++
=
+
+
3
2
2
1
sin( )
cos( )
mr
a a mr an n n
= −
+
Angular Modulation
Wave length Modulation
▪ The momentum mismatch is compensated by diffraction using a metallic diffraction grating
▪ The resonant transfer of optical energy into an SPP is observed as a dip in the angular or wavelength spectrum of reflected light
▪ Light propagates into the core through total internal
reflection and generates an evanescent field in the
vicinity of the waveguide boundary, which induces SPR
at the interface between the metal film and the sensing
medium
▪ Provides highly integrated, multichannel, and robust
sensing devices
The expression for the sensitivity is obtained by differentiating
resonant condition with respect to , and na
-grating period
Wave guide-based sensor
▪ Planar waveguide configuration - unable to
interrogate the incident angle scanning
▪ Wavelength interrogation is the only option for the
signal acquisition technique
11. 1. B. Liedberg, C. Nylander, I. Lunstrom, “Surface Plasmon resonance for gas detection and biosensing”, Sens. Actuat. 4.p.299(1983).
2. Briliant Adhi Prabowo, Agnes Purwidyantri and Kou-Chen Liu, Surface Plasmon Resonance Optical Sensor: A Review on Light Source Technology, Biosensors 2018,
8, 80
3. Shaoqing cao, Yu shao, Ying wang, Tiesheng wu, Longfei zhang, Yijian huang, Feng zhang, Changrui liao, Jun he, and Yiping wang, highly sensitive surface plasmon
resonance biosensor based on a low-index polymer optical fiber Vol. 26, No. 4 2018 OPTICS EXPRESS 3988,
4. Qian, Yifeng; Zeng, Xie; Gao, Yongkang; Li, Hang; Kumar, Sushil; Gan, Qiaoqiang; Cheng, Xuanhong; Bartoli, Filbert J., Intensity-modulated nanoplasmonic
interferometric sensor for MMP-9 detection, Lab Chip ; 19(7): 1267-1276, 2019 03 27.
5. Ahmmed A.RifataRajibAhmedbAli K.YetisencdHaiderButtbAydinSabouribG. AmouzadMahdirajieSeok HyunYuncdF.R. MahamdAdikana, Photonic crystal fiber based
plasmonic sensors, Sensors and Actuators B: Chemical, Volume 243, May 2017, 311-325
6. Xiang Zhao 1 , Tianye Huang 1,* ID , Perry Shum Ping 2 , Xu Wu 1 , Pan Huang 1 , Jianxing Pan 1 , Yiheng Wu 1 and Zhuo Cheng 1 Sensitivity Enhancement in
Surface Plasmon Resonance Biochemical Sensor Based on Transition Metal Dichalcogenides/Graphene Heterostructure, Sensors 2018, 18, 2056;
doi:10.3390/s18072056
7. DONGPING WANG, 1 FONG-CHUEN LOO, 2,3 HENGJI CONG, 2 WEI LIN, 1 SIU KAI KONG, 3 YEUNG YAM, 1 SHIH-CHI CHEN, 1,* AND HO PUI HO2,
Real-time multi-channel SPR sensing based on DMD-enabled angular interrogation Vol. 26, No. 19 | 17 Sep 2018 | OPTICS EXPRESS 24627
8. Jir'ı´ Homola, Ivo Koudela, Sinclair S. Yee, “Surface plasmon resonance sensors based on diffraction gratings and prism couplers: sensitivity comparison”, Sensors and
Actuators B 54 (1999) 16–24
9. Jianjun Cao, Yuan Sun, Yan Kong and Weiying Qian, “The Sensitivity of Grating-Based SPR Sensors with Wavelength Interrogation” Sensors 2019, 19, 405;
10.F. Wu, P. A. Thomas, V. G. Kravets, H. O. Arola, M. Soikkeli, K. Iljin, G. Kim, M. Kim,H. S. Shin D. V. Andreeva, C. Neumann, M. Küllmer, A. Turchanin, D. De
Fazio ,O. Balci , V. Babenko, B. Luo, I. Goykhman, S. Hofmann, A. C. Ferrari K. S. Novoselov & A. N. Grigorenko” Layered material latform for surface plasmon
resonance biosensing”Scientific Reports | (2019) 9:20286
11.G. Ruffato, G. Zacco and F. Romanato, Innovative Exploitation of Grating-Coupled Surface Plasmon Resonance for Sensing, http://dx.doi.org/10.5772/51044
12.Radan Slavik, Jiri Homola, Jiri Ctyroky, Eduard Brynda, Novel Spectral Fiber Optic Sensor based on Surface Plasmon Resonance, Sensors and Actuators
B, 74, 106-111
References