Vegetos/Vegetos - International journal of plant research, Mar 18, 2024
Genetic diversity is crucial for the conservation and improvement of plant populations. In this s... more Genetic diversity is crucial for the conservation and improvement of plant populations. In this study, we used molecular and phenotypic profiling to determine the genetic diversity and relationships among Egyptian Vigna populations, including V. radiata, V. unguiculata, and V. luteola. The samples were profiled using amino acids and rbcL sequences, and the data were analyzed to determine the similarities among the taxa. The results showed a high degree of similarity between V. radiata and V. unguiculata, followed by V. luteola and V. unguiculata, and V. luteola and V. radiata. These taxa are closely related to V. unguiculata subsp. cylindrical and V. oblongifolia. Our findings suggest that molecular- and phenotypic-based profiling can help in prioritizing different disciplines based on assigning them to the proper taxonomic position through extra collections and management. These findings have practical implications for the management of Egyptian Vigna populations, as they can inform conservation and crop improvement efforts. Further research in this area could help preserve the wealth of germplasm for biodiversity and contribute to the sustainable use of plant resources.
Genetic diversity is crucial for the conservation and improvement of plant populations. In this s... more Genetic diversity is crucial for the conservation and improvement of plant populations. In this study, we used molecular and phenotypic profiling to determine the genetic diversity and relationships among Egyptian Vigna populations, including V. radiata, V. unguiculata, and V. luteola. The samples were profiled using amino acids and rbcL sequences, and the data were analyzed to determine the similarities among the taxa. The results showed a high degree of similarity between V. radiata and V. unguiculata, followed by V. luteola and V. unguiculata, and V. luteola and V. radiata. These taxa are closely related to V. unguiculata subsp. cylindrical and V. oblongifolia. Our findings suggest that molecular- and phenotypic-based profiling can help in prioritizing different disciplines based on assigning them to the proper taxonomic position through extra collections and management. These findings have practical implications for the management of Egyptian Vigna populations, as they can inform conservation and crop improvement efforts. Further research in this area could help preserve the wealth of germplasm for biodiversity and contribute to the sustainable use of plant resources.
Coriandrum sativum L. is a globally significant economic herb with medicinal and aromatic propert... more Coriandrum sativum L. is a globally significant economic herb with medicinal and aromatic properties. While coriander leaf blight disease was previously confined to India and the USA, this study presents new evidence of its outbreak in Africa and the Middle East caused by Alternaria dauci. Infected leaves display irregular chlorotic to dark brown necrotic lesions along their edges, resulting in leaf discoloration, collapse, and eventual death. The disease also impacts inflorescences and seeds, significantly reducing seed quality. Koch’s postulates confirmed the pathogenicity of the fungus through the re-isolation of A. dauci from artificially infected leaves, and its morphology aligns with typical A. dauci features. Notably, this study identified strong lytic activity (cellulase: 23.76 U, xylanase: 12.83 U, pectinase: 51.84 U, amylase: 9.12 U, and proteinase: 5.73 U), suggesting a correlation with pathogenicity. Molecular characterization using ITS (ON171224) and the specific Alt-a-...
The objectives of the present study were to measure the genetic diversity among eight inbred line... more The objectives of the present study were to measure the genetic diversity among eight inbred lines of maize using ISSR markers and the correlation coefficients between genetic diversity and each of heterosis and mean performance of hybrids for grain yield. Ten ISSR primers were used in the detection of polymorphism of the eight inbred lines in a laboratorial experiment. Heterosis and mean performance of grain yield/ha in their F1 diallel crosses were measured in a 2-year field experiment using a randomized complete blocks design with three replications. Based on ISSR markers, the genetic similarity coefficients among the eight maize inbred lines ranged from 0.798 (between L17 and IL53) to 0.943 (between IL80 and IL84) with an average of 0.869. Unique bands associated with maize inbred lines were identified. The results revealed that the genetic diversity among the inbred lines based on ISSR markers showed a significant, and negative relationship with mid-parent heterosis and mean pe...
Faba bean is considered one of the most prominent grain legumes, with high protein content for hu... more Faba bean is considered one of the most prominent grain legumes, with high protein content for human food consumption and livestock feed. The present study evaluated the nature of gene action and determined the genetic diversity among different populations of three crosses for resistance to foliar diseases at the molecular level. Analysis of variance exposed significant differences among the generations for all measured traits. Both dominance and additive gene effects were essential, but dominance genes, for the most part, exhibited greater effects than additive ones. This indicates an essential role for dominant genes alongside the additives one in inheriting such traits. The third cross (Marina × Giza 40) gave desired significant and positive (additive × additive) values for the number of pods/plant, seeds/plant, and seed yield/plant, in addition to desirable negative values for chocolate spot and rust characteristics. Furthermore, assessing the lines under study using seven SCoT ...
Background Wheat is a major cereal that can narrow the gap between the increasing human populatio... more Background Wheat is a major cereal that can narrow the gap between the increasing human population and food production. In this connection, assessing genetic diversity and conserving wheat genetic resources for future exploitation is very important for breeding new cultivars that may withstand the expected climate change. The current study evaluates the genetic diversity in selected wheat cultivars using ISSR and SCoT markers, the rbcL and matK chloroplast DNA barcoding, and grain surface sculpture characteristics. We anticipate that these objectives may prioritize using the selected cultivars to improve wheat production. The selected collection of cultivars may lead to the identification of cultivars adapted to a broad spectrum of climatic environments. Results Multivariate clustering analyses of the ISSR and SCoT DNA fingerprinting polymorphism grouped three Egyptian cultivars with cultivar El-Nielain from Sudan, cultivar Aguilal from Morocco, and cultivar Attila from Mexico. In t...
Background Phytoremediation is determined as an emerging green technology suitable for the safe r... more Background Phytoremediation is determined as an emerging green technology suitable for the safe remediation and restoration of polluted terrestrial and aquatic environments. In this study, the assessment of an ornamental plant, Vinca rosea L., as a phytoremediator of crude oil in polluted soils was conducted. In an open greenhouse experiment, plants were raised in sandy-clayey soils treated with 1, 3, 5, and 7% oil by weight. The experiment was conducted over 5 months. Results Total petroleum hydrocarbon (TPH) degradation percentage by V. rosea after a 5-month growth period ranged from 86.83 ± 0.44% to 59.05% ± 0.45% in soil treated with 1 and 7%, respectively. Plants raised in polluted soils demonstrated a dramatic reduction in germination rates, in addition to growth inhibition outcomes shown from decreased plant height. An increase in branching was observed with an increase in oil pollution percentages. Moreover, the phytomass allocated to the leaves was higher, while the phytoma...
BACKGROUND Germplasm identification is an essential connection linking the conservation and explo... more BACKGROUND Germplasm identification is an essential connection linking the conservation and exploitation of crop genetic resources in several plant breeding programs. This study highlights the biochemical and molecular variations in a collection of pumpkin genotypes representing four climate zones. The information could help improve germplasm management and sustainable exploitation of the neglected genotypes. METHODS AND RESULTS Chemical characterization and genetic diversity among nine Egyptian landraces of pumpkin (Cucurbita moschata Duchesne) were estimated using Diode Array (DDA) Near Infra-Red (NIR) technology and the Inter simple Sequence Repeat markers (ISSR). Pumpkin seeds were collected from various geographical parts of Egypt. The spectroscopic properties of pumpkin seeds were used to quantify the fat, moisture, protein, ash, fiber, and total carbohydrate contents. The ten ISSR primers generated a total number of 46 genotype-specific bands, and the total polymorphism produced in the tested landraces was 63.58%. Based on the ISSR data, the polymorphism analysis divided the nine pumpkin landraces into two main groups, two subgroups, and four sub subgroups. The most diverse pumpkin landraces were Alexandria and Sohag, with a similarity percentage of 49.6%. However, the highest calculated similarity value was 88.3% between Matruh and Gharbia. The resultant genotype-specific bands can be used as markers for future genotypic characterization of pumpkins. CONCLUSIONS The study results could be helpful in the chemical phenotypic characterization and the parental selection and planning for future breeding programs for pumpkin improvement.
Fusarium wilt caused by Fusarium oxysporum (Fo) is the maximum critical soil borne diseases of ch... more Fusarium wilt caused by Fusarium oxysporum (Fo) is the maximum critical soil borne diseases of chickpea in Egypt and many other countries in the world. Sixteen F. oxysporum(Fo) isolates were collected from 9 Governorates in Egypt isolated from wilted chickpea plants. Identification of these isolates was achieved by variation in morphological characters i.e., growth rate, growth habits, pigments, sporulation, and the morphological identifications were further confirmed by molecular method using specific ITS primers. Virulence of these isolates was classified as high virulence (Fo7, Fo8 and Fo10), moderate (Fo2, Fo3, Fo6, Fo9, Fo11, Fo13, Fo14, Fo15 and Fo16) and low (Fo1, Fo4, Fo5, Fo12). Host response of chickpea cultivars infected with Fo7 showed a moderate resistance (Giza-2 and Giza-531) and susceptible ones (Giza-195, Giza-1 and Giza-88). Mechanism underline induce disease resistance revealed that resistant chickpea cultivars contained high activities peroxidase, polyphenol oxid...
A cDNA- start codon-targeted polymorphism (cDNA-SCoT) marker was employed to isolate and characte... more A cDNA- start codon-targeted polymorphism (cDNA-SCoT) marker was employed to isolate and characterize differentially expressed sequence tags (ESTs) in response to drought stress in wheat ( Triticum aestivum L.) at seedling stage, cultivar Giza 186. Three weeks old wheat seedlings were subjected to drought stress, the seedlings sampled at different time points (1 h and 6 h) after exposed to drought stress with three biological replicas for each treatment, and untreated seedlings were used as control. An array of 17 differentially expressed EST fragments in drought-stressed plants comparing to control was obtained, and characterized using the bioinformatics analysis. A cDNA-SCoT marker showed a variation in different gene expressions profiling between treated and untreated seedlings under drought stress.
Journal of Genetic Engineering and Biotechnology, 2020
Background Anthropogenic activity, climate change, pollution, and exploitation of natural resourc... more Background Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity. Silene schimperiana is an endangered plant species in Egypt and is endemic to the high mountain of Saint Katherine Protected Area in southern Sinai. The purpose of the study was the ex situ conservation of Silene schimperiana through in vitro propagation and DNA barcode analysis. Results To develop an efficient ex situ conservation program of the plant, in vitro propagation protocol has been achieved from shoot tip and stem nodal segment explants of in vitro germinated seedlings. Explants were established in vitro on Murashige and Skoog (MS) medium supplemented with 2.89 μM gibberellic acid (GA3), 1.08 μM α-naphthaleneacetic acid (NAA), and 1.16 μM kinetin (Kin). The highest number of axillary shoots (9.27) was obtained when they were transferred to MS medium supplemented with 4.48 μM 6-benzyl adenine (BA). Hundred percent of...
Chickpea is an important crop that delivers nutritious food to the increasing global community an... more Chickpea is an important crop that delivers nutritious food to the increasing global community and it will become increasingly popular as a result of climate change. Our objective was to use comprehensive data analysis to locate and identify candidate genes for fungal disease resistance. We used a comprehensive bioinformatics pipeline of sequence alignment, phylogenetic analysis, protein chemical and physical properties assessment and domain structure classification. In order to study gene evolution and genetic diversity, we compared these genes with known anti-fungal genes in different species of plants. A total of 19721 protein sequences belonging to 187 plant species have been downloaded from public databases, including the entire chickpea genome. We have successfully identified 23 potential anti-fungal genes in 10 different chromosomes and genomic scaffolds using sequence alignment and gene annotation. Ca2 and Ca6 have the highest number of genes followed by Ca3 and Ca4. Anti-fu...
Saint Katherine is considered a “biodiversity hotspot” because of the high level of endemism of r... more Saint Katherine is considered a “biodiversity hotspot” because of the high level of endemism of reported plant species. In this study, conservation of the endangered endemic plant; Phlomis aurea of Saint Katherine, Southern Sinai, Egypt, was carried out through micropropagation and DNA barcoding. The first efficient micropropagation protocol for Phlomis aurea was established as a mean of ex situ conservation of the plant. Shoot tips and nodal segments of in vitro germinated seedlings were established on Murashige and Skoog medium supplemented with 0.54 µM β-naphthalene acetic acid (NAA) and 2.46 µM N6-(2-isopentenyl) adenine (2iP) in combination with 6-benzylaminopurine (BA) or kinetin (Kin). The medium supplemented with 3.48 µM Kin considered optimum for both explants. For multiplication, BA was the most efficient cytokinin. The percentage of rooted explants reached 100% at the concentration of 14.7 µM indolebutyric acid (IBA), whereas the highest number of roots was recorded for 4...
Accelerating breeding programs require the use of efficient molecular tools and enhance the diver... more Accelerating breeding programs require the use of efficient molecular tools and enhance the diversity of local faba bean cultivars. The aim of this study is to develop a new genetic database of available SSR primers, to classify these PCR primers according to the target genes and their respective cell processes, to assess the diversity and efficacy of the microsatellite markers applied to faba bean and to study the genetic structure of some selected faba bean cultivars. Approximately 75,605 and 148,196 previously published genomic and transcriptomic sequences were used to detect possible simple sequence repetitions in faba genomic content. The number of identified SSRs was 25502 and 12319, where the distribution to different repeat type classes shows that, the trinucleotide was the highest number of repeat counts followed by dinucleotide repeats. These genic SSR sequences were used to design 1091 PCR primers, out of which only 238 (21.8%) primers target genomic sequences and the oth...
THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY, 2015
Species characterization in the family Characeae is generally based on morphological characterist... more Species characterization in the family Characeae is generally based on morphological characteristics. The dioecious charophyte green algal genus Chara L. comprises of several species, of which high levels of morphological resemblance were recorded in this study. Four Chara samples were collected from different habitats in Egypt and morphologically studied. SSR-PCR and ISSR-PCR based techniques were performed to differentiate and characterize genetic diversity in the collected species. Two SSR and eight ISSR primers were used. Genetic differentiation of two Chara species (Sp.1 and Sp.2) was assessed by analyzing two polymorphic microsatellite (SSR) loci previously identified and developed from C. canescens. Additionally, similarity matrix and cluster analysis revealed by ISSR markers suggested separation of three Chara species (Sp.1, Sp.2, and Sp.3) away from the fourth one (Sp.4). The results indicated that applied SSR and ISSR markers most likely suitable for characterization at ge...
Vegetos/Vegetos - International journal of plant research, Mar 18, 2024
Genetic diversity is crucial for the conservation and improvement of plant populations. In this s... more Genetic diversity is crucial for the conservation and improvement of plant populations. In this study, we used molecular and phenotypic profiling to determine the genetic diversity and relationships among Egyptian Vigna populations, including V. radiata, V. unguiculata, and V. luteola. The samples were profiled using amino acids and rbcL sequences, and the data were analyzed to determine the similarities among the taxa. The results showed a high degree of similarity between V. radiata and V. unguiculata, followed by V. luteola and V. unguiculata, and V. luteola and V. radiata. These taxa are closely related to V. unguiculata subsp. cylindrical and V. oblongifolia. Our findings suggest that molecular- and phenotypic-based profiling can help in prioritizing different disciplines based on assigning them to the proper taxonomic position through extra collections and management. These findings have practical implications for the management of Egyptian Vigna populations, as they can inform conservation and crop improvement efforts. Further research in this area could help preserve the wealth of germplasm for biodiversity and contribute to the sustainable use of plant resources.
Genetic diversity is crucial for the conservation and improvement of plant populations. In this s... more Genetic diversity is crucial for the conservation and improvement of plant populations. In this study, we used molecular and phenotypic profiling to determine the genetic diversity and relationships among Egyptian Vigna populations, including V. radiata, V. unguiculata, and V. luteola. The samples were profiled using amino acids and rbcL sequences, and the data were analyzed to determine the similarities among the taxa. The results showed a high degree of similarity between V. radiata and V. unguiculata, followed by V. luteola and V. unguiculata, and V. luteola and V. radiata. These taxa are closely related to V. unguiculata subsp. cylindrical and V. oblongifolia. Our findings suggest that molecular- and phenotypic-based profiling can help in prioritizing different disciplines based on assigning them to the proper taxonomic position through extra collections and management. These findings have practical implications for the management of Egyptian Vigna populations, as they can inform conservation and crop improvement efforts. Further research in this area could help preserve the wealth of germplasm for biodiversity and contribute to the sustainable use of plant resources.
Coriandrum sativum L. is a globally significant economic herb with medicinal and aromatic propert... more Coriandrum sativum L. is a globally significant economic herb with medicinal and aromatic properties. While coriander leaf blight disease was previously confined to India and the USA, this study presents new evidence of its outbreak in Africa and the Middle East caused by Alternaria dauci. Infected leaves display irregular chlorotic to dark brown necrotic lesions along their edges, resulting in leaf discoloration, collapse, and eventual death. The disease also impacts inflorescences and seeds, significantly reducing seed quality. Koch’s postulates confirmed the pathogenicity of the fungus through the re-isolation of A. dauci from artificially infected leaves, and its morphology aligns with typical A. dauci features. Notably, this study identified strong lytic activity (cellulase: 23.76 U, xylanase: 12.83 U, pectinase: 51.84 U, amylase: 9.12 U, and proteinase: 5.73 U), suggesting a correlation with pathogenicity. Molecular characterization using ITS (ON171224) and the specific Alt-a-...
The objectives of the present study were to measure the genetic diversity among eight inbred line... more The objectives of the present study were to measure the genetic diversity among eight inbred lines of maize using ISSR markers and the correlation coefficients between genetic diversity and each of heterosis and mean performance of hybrids for grain yield. Ten ISSR primers were used in the detection of polymorphism of the eight inbred lines in a laboratorial experiment. Heterosis and mean performance of grain yield/ha in their F1 diallel crosses were measured in a 2-year field experiment using a randomized complete blocks design with three replications. Based on ISSR markers, the genetic similarity coefficients among the eight maize inbred lines ranged from 0.798 (between L17 and IL53) to 0.943 (between IL80 and IL84) with an average of 0.869. Unique bands associated with maize inbred lines were identified. The results revealed that the genetic diversity among the inbred lines based on ISSR markers showed a significant, and negative relationship with mid-parent heterosis and mean pe...
Faba bean is considered one of the most prominent grain legumes, with high protein content for hu... more Faba bean is considered one of the most prominent grain legumes, with high protein content for human food consumption and livestock feed. The present study evaluated the nature of gene action and determined the genetic diversity among different populations of three crosses for resistance to foliar diseases at the molecular level. Analysis of variance exposed significant differences among the generations for all measured traits. Both dominance and additive gene effects were essential, but dominance genes, for the most part, exhibited greater effects than additive ones. This indicates an essential role for dominant genes alongside the additives one in inheriting such traits. The third cross (Marina × Giza 40) gave desired significant and positive (additive × additive) values for the number of pods/plant, seeds/plant, and seed yield/plant, in addition to desirable negative values for chocolate spot and rust characteristics. Furthermore, assessing the lines under study using seven SCoT ...
Background Wheat is a major cereal that can narrow the gap between the increasing human populatio... more Background Wheat is a major cereal that can narrow the gap between the increasing human population and food production. In this connection, assessing genetic diversity and conserving wheat genetic resources for future exploitation is very important for breeding new cultivars that may withstand the expected climate change. The current study evaluates the genetic diversity in selected wheat cultivars using ISSR and SCoT markers, the rbcL and matK chloroplast DNA barcoding, and grain surface sculpture characteristics. We anticipate that these objectives may prioritize using the selected cultivars to improve wheat production. The selected collection of cultivars may lead to the identification of cultivars adapted to a broad spectrum of climatic environments. Results Multivariate clustering analyses of the ISSR and SCoT DNA fingerprinting polymorphism grouped three Egyptian cultivars with cultivar El-Nielain from Sudan, cultivar Aguilal from Morocco, and cultivar Attila from Mexico. In t...
Background Phytoremediation is determined as an emerging green technology suitable for the safe r... more Background Phytoremediation is determined as an emerging green technology suitable for the safe remediation and restoration of polluted terrestrial and aquatic environments. In this study, the assessment of an ornamental plant, Vinca rosea L., as a phytoremediator of crude oil in polluted soils was conducted. In an open greenhouse experiment, plants were raised in sandy-clayey soils treated with 1, 3, 5, and 7% oil by weight. The experiment was conducted over 5 months. Results Total petroleum hydrocarbon (TPH) degradation percentage by V. rosea after a 5-month growth period ranged from 86.83 ± 0.44% to 59.05% ± 0.45% in soil treated with 1 and 7%, respectively. Plants raised in polluted soils demonstrated a dramatic reduction in germination rates, in addition to growth inhibition outcomes shown from decreased plant height. An increase in branching was observed with an increase in oil pollution percentages. Moreover, the phytomass allocated to the leaves was higher, while the phytoma...
BACKGROUND Germplasm identification is an essential connection linking the conservation and explo... more BACKGROUND Germplasm identification is an essential connection linking the conservation and exploitation of crop genetic resources in several plant breeding programs. This study highlights the biochemical and molecular variations in a collection of pumpkin genotypes representing four climate zones. The information could help improve germplasm management and sustainable exploitation of the neglected genotypes. METHODS AND RESULTS Chemical characterization and genetic diversity among nine Egyptian landraces of pumpkin (Cucurbita moschata Duchesne) were estimated using Diode Array (DDA) Near Infra-Red (NIR) technology and the Inter simple Sequence Repeat markers (ISSR). Pumpkin seeds were collected from various geographical parts of Egypt. The spectroscopic properties of pumpkin seeds were used to quantify the fat, moisture, protein, ash, fiber, and total carbohydrate contents. The ten ISSR primers generated a total number of 46 genotype-specific bands, and the total polymorphism produced in the tested landraces was 63.58%. Based on the ISSR data, the polymorphism analysis divided the nine pumpkin landraces into two main groups, two subgroups, and four sub subgroups. The most diverse pumpkin landraces were Alexandria and Sohag, with a similarity percentage of 49.6%. However, the highest calculated similarity value was 88.3% between Matruh and Gharbia. The resultant genotype-specific bands can be used as markers for future genotypic characterization of pumpkins. CONCLUSIONS The study results could be helpful in the chemical phenotypic characterization and the parental selection and planning for future breeding programs for pumpkin improvement.
Fusarium wilt caused by Fusarium oxysporum (Fo) is the maximum critical soil borne diseases of ch... more Fusarium wilt caused by Fusarium oxysporum (Fo) is the maximum critical soil borne diseases of chickpea in Egypt and many other countries in the world. Sixteen F. oxysporum(Fo) isolates were collected from 9 Governorates in Egypt isolated from wilted chickpea plants. Identification of these isolates was achieved by variation in morphological characters i.e., growth rate, growth habits, pigments, sporulation, and the morphological identifications were further confirmed by molecular method using specific ITS primers. Virulence of these isolates was classified as high virulence (Fo7, Fo8 and Fo10), moderate (Fo2, Fo3, Fo6, Fo9, Fo11, Fo13, Fo14, Fo15 and Fo16) and low (Fo1, Fo4, Fo5, Fo12). Host response of chickpea cultivars infected with Fo7 showed a moderate resistance (Giza-2 and Giza-531) and susceptible ones (Giza-195, Giza-1 and Giza-88). Mechanism underline induce disease resistance revealed that resistant chickpea cultivars contained high activities peroxidase, polyphenol oxid...
A cDNA- start codon-targeted polymorphism (cDNA-SCoT) marker was employed to isolate and characte... more A cDNA- start codon-targeted polymorphism (cDNA-SCoT) marker was employed to isolate and characterize differentially expressed sequence tags (ESTs) in response to drought stress in wheat ( Triticum aestivum L.) at seedling stage, cultivar Giza 186. Three weeks old wheat seedlings were subjected to drought stress, the seedlings sampled at different time points (1 h and 6 h) after exposed to drought stress with three biological replicas for each treatment, and untreated seedlings were used as control. An array of 17 differentially expressed EST fragments in drought-stressed plants comparing to control was obtained, and characterized using the bioinformatics analysis. A cDNA-SCoT marker showed a variation in different gene expressions profiling between treated and untreated seedlings under drought stress.
Journal of Genetic Engineering and Biotechnology, 2020
Background Anthropogenic activity, climate change, pollution, and exploitation of natural resourc... more Background Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity. Silene schimperiana is an endangered plant species in Egypt and is endemic to the high mountain of Saint Katherine Protected Area in southern Sinai. The purpose of the study was the ex situ conservation of Silene schimperiana through in vitro propagation and DNA barcode analysis. Results To develop an efficient ex situ conservation program of the plant, in vitro propagation protocol has been achieved from shoot tip and stem nodal segment explants of in vitro germinated seedlings. Explants were established in vitro on Murashige and Skoog (MS) medium supplemented with 2.89 μM gibberellic acid (GA3), 1.08 μM α-naphthaleneacetic acid (NAA), and 1.16 μM kinetin (Kin). The highest number of axillary shoots (9.27) was obtained when they were transferred to MS medium supplemented with 4.48 μM 6-benzyl adenine (BA). Hundred percent of...
Chickpea is an important crop that delivers nutritious food to the increasing global community an... more Chickpea is an important crop that delivers nutritious food to the increasing global community and it will become increasingly popular as a result of climate change. Our objective was to use comprehensive data analysis to locate and identify candidate genes for fungal disease resistance. We used a comprehensive bioinformatics pipeline of sequence alignment, phylogenetic analysis, protein chemical and physical properties assessment and domain structure classification. In order to study gene evolution and genetic diversity, we compared these genes with known anti-fungal genes in different species of plants. A total of 19721 protein sequences belonging to 187 plant species have been downloaded from public databases, including the entire chickpea genome. We have successfully identified 23 potential anti-fungal genes in 10 different chromosomes and genomic scaffolds using sequence alignment and gene annotation. Ca2 and Ca6 have the highest number of genes followed by Ca3 and Ca4. Anti-fu...
Saint Katherine is considered a “biodiversity hotspot” because of the high level of endemism of r... more Saint Katherine is considered a “biodiversity hotspot” because of the high level of endemism of reported plant species. In this study, conservation of the endangered endemic plant; Phlomis aurea of Saint Katherine, Southern Sinai, Egypt, was carried out through micropropagation and DNA barcoding. The first efficient micropropagation protocol for Phlomis aurea was established as a mean of ex situ conservation of the plant. Shoot tips and nodal segments of in vitro germinated seedlings were established on Murashige and Skoog medium supplemented with 0.54 µM β-naphthalene acetic acid (NAA) and 2.46 µM N6-(2-isopentenyl) adenine (2iP) in combination with 6-benzylaminopurine (BA) or kinetin (Kin). The medium supplemented with 3.48 µM Kin considered optimum for both explants. For multiplication, BA was the most efficient cytokinin. The percentage of rooted explants reached 100% at the concentration of 14.7 µM indolebutyric acid (IBA), whereas the highest number of roots was recorded for 4...
Accelerating breeding programs require the use of efficient molecular tools and enhance the diver... more Accelerating breeding programs require the use of efficient molecular tools and enhance the diversity of local faba bean cultivars. The aim of this study is to develop a new genetic database of available SSR primers, to classify these PCR primers according to the target genes and their respective cell processes, to assess the diversity and efficacy of the microsatellite markers applied to faba bean and to study the genetic structure of some selected faba bean cultivars. Approximately 75,605 and 148,196 previously published genomic and transcriptomic sequences were used to detect possible simple sequence repetitions in faba genomic content. The number of identified SSRs was 25502 and 12319, where the distribution to different repeat type classes shows that, the trinucleotide was the highest number of repeat counts followed by dinucleotide repeats. These genic SSR sequences were used to design 1091 PCR primers, out of which only 238 (21.8%) primers target genomic sequences and the oth...
THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY, 2015
Species characterization in the family Characeae is generally based on morphological characterist... more Species characterization in the family Characeae is generally based on morphological characteristics. The dioecious charophyte green algal genus Chara L. comprises of several species, of which high levels of morphological resemblance were recorded in this study. Four Chara samples were collected from different habitats in Egypt and morphologically studied. SSR-PCR and ISSR-PCR based techniques were performed to differentiate and characterize genetic diversity in the collected species. Two SSR and eight ISSR primers were used. Genetic differentiation of two Chara species (Sp.1 and Sp.2) was assessed by analyzing two polymorphic microsatellite (SSR) loci previously identified and developed from C. canescens. Additionally, similarity matrix and cluster analysis revealed by ISSR markers suggested separation of three Chara species (Sp.1, Sp.2, and Sp.3) away from the fourth one (Sp.4). The results indicated that applied SSR and ISSR markers most likely suitable for characterization at ge...
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Papers by Shafik D. Ibrahim