Niels Nørkjær Johannsen

Significance We sequenced the genomes of 15 skeletons from a 5,000-y-old mass grave in Poland associated with the Globular Amphora culture. All individuals had been brutally killed by blows to the head, but buried with great care. Genome-wide analyses demonstrate that this was a large extended family and that the people who buried them knew them well: mothers are buried with their children, and siblings next to each other. From a population genetic viewpoint, the individuals are clearly distinct from neighboring Corded Ware groups because of their lack of steppe-related ancestry. Although the reason for the massacre is unknown, it is possible that it was connected with the expansion of Corded Ware groups, which may have resulted in violent conflict.

The transitions from foraging to farming and later to pastoralism in Stone Age Eurasia (c. 113 thousand years before present, BP) represent some of the most dramatic lifestyle changes in human evolution. We sequenced 317 genomes of primarily Mesolithic and Neolithic individuals from across Eurasia combined with radiocarbon dates, stable isotope data, and pollen records. Genome imputation and co-analysis with previously published shotgun sequencing data resulted in >1600 complete ancient genome sequences offering fine-grained resolution into the Stone Age populations. We observe that: 1) Hunter-gatherer groups were more genetically diverse than previously known, and deeply divergent between western and eastern Eurasia. 2) We identify hitherto genetically undescribed hunter-gatherers from the Middle Don region that contributed ancestry to the later Yamnaya steppe pastoralists; 3) The genetic impact of the Neolithic transition was highly distinct, east and west of a boundary zone extending from the Black Sea to the Baltic. Large-scale shifts in genetic ancestry occurred to the west of this “Great Divide”, including an almost complete replacement of hunter-gatherers in Denmark, while no substantial ancestry shifts took place during the same period to the east. This difference is also reflected in genetic relatedness within the populations, decreasing substantially in the west but not in the east where it remained high until c. 4,000 BP; 4) The second major genetic transformation around 5,000 BP happened at a much faster pace with Steppe-related ancestry reaching most parts of Europe within 1,000years. Local Neolithic farmers admixed with incoming pastoralists in eastern, western, and southern Europe whereas Scandinavia experienced another near-complete population replacement. Similar dramatic turnover-patterns are evident in western Siberia; 5) Extensive regional differences in the ancestry components involved in these early events remain visible to this day, even within countries. Neolithic farmer ancestry is highest in southern and eastern England while Steppe-related ancestry is highest in the Celtic populations of Scotland, Wales, and Cornwall (this research has been conducted using the UK Biobank resource); 6) Shifts in diet, lifestyle and environment introduced new selection pressures involving at least 21 genomic regions. Most such variants were not universally selected across populations but were only advantageous in particular ancestral backgrounds. Contrary to previous claims, we find that selection on the FADS regions, associated with fatty acid metabolism, began before the Neolithisation of Europe. Similarly, the lactase persistence allele started increasing in frequency before the expansion of Steppe-related groups into Europe and has continued to increase up to the present. Along the genetic cline separating Mesolithic hunter-gatherers from Neolithic farmers, we find significant correlations with trait associations related to skin disorders, diet and lifestyle and mental health status, suggesting marked phenotypic differences between these groups with very different lifestyles. This work provides new insights into major transformations in recent human evolution, elucidating the complex interplay between selection and admixture that shaped patterns of genetic variation in modern populations.

Cultural evolutionary theory conceptualises culture as an information-transmission system whose dynamics take on evolutionary properties. Within this framework, however, innovation has been likened to random
mutations, reducing its occurrence to chance or fortuitous transmission error. In introducing the special collection on children and innovation, we here place object play and play objects – especially functional miniatures – from carefully chosen archaeological contexts in a niche construction perspective. Given that play, including object play, is ubiquitous in human societies, we suggest that plaything construction, provisioning and use have, over evolutionary timescales, paid substantial selective dividends
via ontogenetic niche modification. Combining findings from cognitive science, ethology and ethnography with insights into hominin early developmental life-history, we show how play objects and object
play probably had decisive roles in the emergence of innovative capabilities. Importantly, we argue that closer attention to play objects can go some way towards addressing changes in innovation rates that occurred throughout human biocultural evolution and why innovations are observable within certain technological domains but not others.

The third millennium BCE was a period of major cultural and demographic changesin Europethat signaled the beginningof the Bronze Age. People from the Pontic steppe expanded westward, leading to the formation of the Corded Ware complex and transforming the genetic landscape of Europe. At the time, the Globular Amphora culture (3300–2700 BCE) existed over large parts of Central and Eastern Europe, but little is known about their interaction with neighboring Corded Ware groups and steppe societies. Here we present a detailed study of a Late Neolithic mass grave from southern Poland belonging to the Globular Amphora culture and containing the remains of 15 men, women, and children, all killed by blows to the head. We sequenced their genomes tobetween1.1- and3.9-foldcoverageandperformed kinshipanalyses that demonstrate that the individuals belonged to a large extended family. The bodies had been carefully laid out according to kin relationships by someone who evidently knew the deceased. From a population genetic viewpoint, the people from Koszyce are clearly distinct from neighboring Corded Ware groups because of their lack of steppe-related ancestry. Although the reason for the massacre is unknown, it is possible that it was connected with the expansion of Corded Ware groups, which may have resulted in competition for resources and violent conflict. Together with the archaeological evidence, these analyses provide an unprecedented level of insight into the kinship structure and social behavior of a Late Neolithic community.

We sequenced the genomes of 15 skeletons from a 5,000-y-old mass grave in Poland associated with the Globular Amphora culture. All individuals had been brutally killed by blows to the head, but buried with great care. Genome-wide analyses demonstrate that this was a large extended family and that the people who buried them knew them well: mothers are buried with their children, and siblings next to each other. From a population genetic viewpoint, the individuals are clearly distinct from neighboring Corded Ware groups because of their lack of steppe-related ancestry. Although the reason for the massacre is unknown, it is possible that it was connected with the expansion of Corded Ware groups, which may have resulted in violent conflict.

In this contribution, we address a major puzzle in the evolution of human material culture: If maturing individuals just learn their parental generation's material culture, then what is the origin of key innovations as documented in the archeological record? We approach this question by coupling a life-history model of the costs and benefits of experimentation with a niche-construction perspective. Niche-construction theory suggests that the behavior of organisms and their modification of the world around them have important evolutionary ramifications by altering developmental settings and selection pressures. Part of Homo sapiens' niche is the active provisioning of children with play objects — sometimes functional miniatures of adult tools — and the encouragement of object play, such as playful knapping with stones. Our model suggests that salient material culture innovation may occur or be primed in a late childhood or adolescence sweet spot when cognitive and physical abilities are sufficiently mature but before the full onset of the concerns and costs associated with reproduction. We evaluate the model against a series of archeological cases and make suggestions for future research.

Over the past decade, the ability to recover whole genomes from ancient remains has emerged as a powerful tool for understanding the human past. From a strictly biological perspective, the sequencing of ancient genomes has resolved the dispute over our evolutionary relationship with Neandertals, revealed the extent of gene flow within and between modern and archaic humans, shed light on genetic and health consequences of this admixture, and uncovered genomic changes in recent human evolution. More generally, the results have made clear that over the course of human history, moving and mating have been more the rule than the exception. The possible benefits of ancient DNA (aDNA) research for archaeology are enormous. Why, then, have aDNA approaches to archaeological questions occasionally raised eyebrows among archaeologists?

Recent genetic, isotopic and linguistic research has dramatically changed our understanding of how the Corded Ware Culture in Europe was formed. Here the authors explain it in terms of local adaptations and interactions between migrant Yamnaya people from the Pontic-Caspian steppe and indigenous North European Neolithic cultures. The original herding economy of the Yamnaya migrants gradually gave way to new practices of crop cultivation, which led to the adoption of new words for those crops. The result of this hybridisation process was the formation of a new material culture, the Corded Ware Culture, and of a new dialect, Proto-Germanic. Despite a degree of hostility between expanding Corded Ware groups and indigenous Neolithic groups, stable isotope data suggest that exogamy provided a mechanism facilitating their integration.

A unique artefact assemblage recovered at the causewayed enclosure of Vasagård on the Baltic island of Bornholm may provide insights into ritual life and underlying patterns of cognition and transmission among the Neolithic population that used this site. Here, more than 400 so-called ‘sun stones’ have emerged – small tablets of shale, sandstone and water-rolled pebbles that have been engraved with a range of motifs. One prevalent motif consists of a circle with radiating lines very similar to what present-day humans would produce if asked to draw a sun, hence the archaeological name; however, a range of other motifs also appear on the stones. All of the engravings are based on a relatively small repertoire and yet, no two stones are identical as the repeated elements are combined in different constellations, creating substantial variability within the material. An explanation for the seeming dichotomy between the normativity that directs the choice of motif, on the one hand, and the relative freedom in its execution, on the other, may potentially be found in the ways that cultural transmission and cognitive processes structured the manufacturing and use of the stones. Drawing on a range of interdisciplinary approaches, we explore the roles of imitation, emulation and active teaching, and how these connect to reproducibility and memorability, in an attempt to understand the apparent combination of structuring and idiosyncrasy. This leads to a number of observations as well as suggestions for further research on these enigmatic stones.

Objective: This study provides a baseline of pathological and sub-pathological changes in the lower-limb bones of a semi-feral herd of domestic cattle. The purpose is to refine an existing method for identifying the use of cattle for traction using zooarchaeological evidence.
Methods: A published recording system for identifying draught cattle was applied to a sample of 15 individuals from Chillingham Park, Northumberland. Correlations were explored between individual pathological index values, the scores obtained for individual pathological/sub-pathological changes, and three biological variables:
age, sex and body size.
Results: Pathological index values in the Chillingham cattle were low. Positive correlations between individual pathological index values and age, sex and body size were identified. Broadening of the distal metacarpal, proximal and distal exostoses in the metatarsal, distal exostoses of the proximal phalanx, and proximal lipping
and exostoses of the distal phalanx, were strongly correlated with age.
Conclusions: Pathological index scores demonstrate that adaptive remodeling of the autopodia is low in a free-ranging population of cattle, supporting the view that more pronounced changes provide useful identifiers of traction use. Application of modified pathological index formulae to nine archaeological sites from England indicated that cattle were only intensively used for traction in the Roman and later medieval periods.
Significance: This study refines the methods used to identify traction in the archaeological record through the consideration of cows and a wider range of ages than has been considered previously.
Limitations: Only 15 individuals from the Chillingham herd were available for analysis.
Suggestions for further research: The refined formulae should be applied to additional archaeological datasets from different regions and time periods to explore the changing exploitation of cattle for traction.