Байкальская нерпа – эндемик и единственное водное млекопитающее озера Байкал. Поскольку озеро включено в Список участков мирового природного наследия ЮНЕСКО, то представляется актуальным изучение его обитателей, в частности такого... more
Байкальская нерпа – эндемик и единственное водное млекопитающее озера Байкал. Поскольку озеро включено в Список участков мирового природного наследия ЮНЕСКО, то представляется актуальным изучение его обитателей, в частности такого уникального животного, как байкальская нерпа, в целях сохранения биологического разнообразия озера. В постнатальном онтогенезе брыжеечные лимфатические узлы байкальской нерпы претерпевают следующие возрастные изменения структурной организации: увеличение относительной площади соединительнотканного компонента; уменьшение доли, занимаемой корковым веществом; уменьшение относительной площади лимфоидных узелков, в том числе вторичных лимфоидных узелков. Проявляется тенденция к возрастному снижению корково-мозгового индекса. В брыжеечных лимфоузлах байкальской нерпы в процессе постнатального онтогенеза происходит снижение числа средних лимфоцитов и ретикулярных клеток, увеличение числа плазматических клеток; уменьшение числа больших лимфоцитов, увеличение содержания малых лимфоцитов в корковом веществе; снижение числа деструктивно измененных и разрушенных клеток в центрах размножения лимфоидных узелков; уменьшение числа малых лимфоцитов в мозговом веществе.
Parasitic worms can survive in the host body for a long time due to their ability to control the immune response. Prostaglandins synthesized by parasites form an important class of immunomodulators. The mechanisms underlying... more
Parasitic worms can survive in the host body for a long time due to their ability to control the immune response. Prostaglandins synthesized by parasites form an important class of immunomodulators. The mechanisms underlying immunoregulation of the host body with prostaglandins are poorly studied, and there is no evidence of prostaglandin functioning in cestodes. Prostaglandin E2 (PG E2) is the most important immunomodulator among prostaglandins. The present study investigated distribution of PG E2 in the organism of Diphyllobothrium dendriticum and influence of PG E2 on the cell culture of sticklebacks head kidney leukocytes. D. dendriticum plerocercoids have been withdrawn from Baikal omul. The sections of plerocercoids were incubated with monoclonal antibodies against PG E2 and α-tubulin, than with secondary antibodies. This was followed by examination with the aid of laser scanning confocal microscope. We have analyzed the frequency of leukocyte subsets (granulocyte to lymphocyt...
Parasitic worms can survive in the host body for a long time due to their ability to control the immune response. Prostaglandins synthesized by parasites form an important class of immunomodulators. The mechanisms underlying... more
Parasitic worms can survive in the host body for a long time due to their ability to control the immune response. Prostaglandins synthesized by parasites form an important class of immunomodulators. The mechanisms underlying immunoregulation of the host body with prostaglandins are poorly studied, and there is no evidence of prostaglandin functioning in cestodes. Prostaglandin E2 (PG E2) is the most important immunomodulator among prostaglandins. The present study investigated distribution of PG E2 in the organism of Diphyllobothrium dendriticum and influence of PG E2 on the cell culture of sticklebacks head kidney leukocytes. D. dendriticum plerocercoids have been withdrawn from Baikal omul. The sections of plerocercoids were incubated with monoclonal antibodies against PG E2 and α-tubulin, than with secondary antibodies. This was followed by examination with the aid of laser scanning confocal microscope. We have analyzed the frequency of leukocyte subsets (granulocyte to lymphocyt...
Research Interests:
Flavobacterium psychrophilum (Fp) is the causative agent of bacterial cold water disease (BCWD) which causes appreciable economic losses in rainbow trout aquaculture. We previously reported development of a genetic line, designated... more
Flavobacterium psychrophilum (Fp) is the causative agent of bacterial cold water disease (BCWD) which causes appreciable economic losses in rainbow trout aquaculture. We previously reported development of a genetic line, designated ARS-Fp-R that exhibits higher survival relative to a susceptible line, designated ARS-Fp-S, following either laboratory or natural on-farm challenge. The objectives of this study were to determine the temporal kinetics of gene expression between experimentally-challenged ARS-Fp-R and ARS-Fp-S fish and the correlation between gene expression and pathogen load. We developed a GeXP multiplex RT-PCR assay to simultaneously examine expression of immune-relevant genes, concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and
Research Interests:
Many helminth parasites have evolved strategies to evade the immune response of their hosts, which includes immunomodulation. Prostaglandin E2 (PGE2) is one of the best-described immunomodulators in mammalian helminth parasite infections.... more
Many helminth parasites have evolved strategies to evade the immune response of their hosts, which
includes immunomodulation. Prostaglandin E2 (PGE2) is one of the best-described immunomodulators in
mammalian helminth parasite infections. We hypothesized that also in teleost fish anti-helminthic
immune responses are regulated via PGE2. We used a model system consisting of the tapeworm Schistocephalus
solidus and its host, the three-spined stickleback (Gasterosteus aculeatus), to investigate in vitro
effects of PGE2 on head kidney leucocytes (HKL) derived from sticklebacks that were experimentally
infected with S. solidus. PGE2 was tested alone or in combination with either S. solidus antigens or
bacterial lipopolysaccharides (LPS). After in vitro culture, cell viability and changes in leucocyte subpopulations
(granulocytes to lymphocytes ratios) were monitored by flow cytometry and HKL were
tested for their capacity to produce reactive oxygen species (ROS) with a chemiluminescence assay. In
short term (2 h) HKL cultures PGE2 did not change the total numbers of live HKL, but the production of
ROS decreased significantly with high (0.1 mmol L1) PGE2 concentrations. In long-term (96 h) cultures
high PGE2 concentrations induced a sharp decrease of leucocytes viability, while low (0.1 pmol L1) and
intermediate (0.1 nmol L1) concentrations of PGE2 caused elevated leucocyte viability compared to
controls. This coincided with reduced ROS production in cultures with high PGE2 and elevated ROS
production in cultures with low PGE2. Granulocyte to lymphocyte ratios increased with high PGE2
concentrations alone and in combination with S. solidus antigens and LPS, most prominently with HKL
from S. solidus infected sticklebacks. The present study supports the hypothesis that PGE2 might be an
immunomodulator in tapewormefish parasiteehost interactions.
includes immunomodulation. Prostaglandin E2 (PGE2) is one of the best-described immunomodulators in
mammalian helminth parasite infections. We hypothesized that also in teleost fish anti-helminthic
immune responses are regulated via PGE2. We used a model system consisting of the tapeworm Schistocephalus
solidus and its host, the three-spined stickleback (Gasterosteus aculeatus), to investigate in vitro
effects of PGE2 on head kidney leucocytes (HKL) derived from sticklebacks that were experimentally
infected with S. solidus. PGE2 was tested alone or in combination with either S. solidus antigens or
bacterial lipopolysaccharides (LPS). After in vitro culture, cell viability and changes in leucocyte subpopulations
(granulocytes to lymphocytes ratios) were monitored by flow cytometry and HKL were
tested for their capacity to produce reactive oxygen species (ROS) with a chemiluminescence assay. In
short term (2 h) HKL cultures PGE2 did not change the total numbers of live HKL, but the production of
ROS decreased significantly with high (0.1 mmol L1) PGE2 concentrations. In long-term (96 h) cultures
high PGE2 concentrations induced a sharp decrease of leucocytes viability, while low (0.1 pmol L1) and
intermediate (0.1 nmol L1) concentrations of PGE2 caused elevated leucocyte viability compared to
controls. This coincided with reduced ROS production in cultures with high PGE2 and elevated ROS
production in cultures with low PGE2. Granulocyte to lymphocyte ratios increased with high PGE2
concentrations alone and in combination with S. solidus antigens and LPS, most prominently with HKL
from S. solidus infected sticklebacks. The present study supports the hypothesis that PGE2 might be an
immunomodulator in tapewormefish parasiteehost interactions.
Research Interests:
"N. M. Biserova and I. A. Kutyrev Biology Bulletin, 2014, Vol. 41, No. 3, pp. 242–250 For the first time, the potential immunomodulators prostaglandin E2 and γaminobutyric acid (GABA) have been revealed in the plerocercoid... more
"N. M. Biserova and I. A. Kutyrev
Biology Bulletin, 2014, Vol. 41, No. 3, pp. 242–250
For the first time, the potential immunomodulators prostaglandin E2 and γaminobutyric acid
(GABA) have been revealed in the plerocercoid Diphyllobothrium dendriticum, which is a parasite in the tis
sues and abdominal cavity of the Baikal omul Coregonus migratorius. The localization of immunomodulators
in parasite tissues was compared with the location of typical markers of the nervous system (serotonin (5HT)
and FMRFamide) and a marker of microtubules (αtubulin). Prostaglandin E2 was revealed in the cells that
are immunoreactive to αtubulin and are situated in the cortical parenchyma outside the central nervous sys
tem (CNS). It is supposed that prostaglandin E2 is produced by the frontal glands and is carried out onto the
tegument surface through specialized ducts. Immunoreaction to GABA was revealed in the central and
peripheral nervous systems. GABAergic neurosomes, the neurites of which form a net on the surface of mus
cle layers and in the subtegument, were revealed in the cerebral ganglion and main nerve cords. The morpho
logical characteristics for the identification of serotoninergic neurons in the CNS were described."
Biology Bulletin, 2014, Vol. 41, No. 3, pp. 242–250
For the first time, the potential immunomodulators prostaglandin E2 and γaminobutyric acid
(GABA) have been revealed in the plerocercoid Diphyllobothrium dendriticum, which is a parasite in the tis
sues and abdominal cavity of the Baikal omul Coregonus migratorius. The localization of immunomodulators
in parasite tissues was compared with the location of typical markers of the nervous system (serotonin (5HT)
and FMRFamide) and a marker of microtubules (αtubulin). Prostaglandin E2 was revealed in the cells that
are immunoreactive to αtubulin and are situated in the cortical parenchyma outside the central nervous sys
tem (CNS). It is supposed that prostaglandin E2 is produced by the frontal glands and is carried out onto the
tegument surface through specialized ducts. Immunoreaction to GABA was revealed in the central and
peripheral nervous systems. GABAergic neurosomes, the neurites of which form a net on the surface of mus
cle layers and in the subtegument, were revealed in the cerebral ganglion and main nerve cords. The morpho
logical characteristics for the identification of serotoninergic neurons in the CNS were described."
Research Interests:
Natalia M Biserova, Ivan A Kutyrev, Kirsten Jensen Journal of Parasitology (Impact Factor: 1.32). 03/2014; DOI:10.1645/13-366.1 The nervous system (NS) of the cestodes Diphyllobothrium dendriticum (Diphyllobothriidea) and... more
Natalia M Biserova, Ivan A Kutyrev, Kirsten Jensen
Journal of Parasitology (Impact Factor: 1.32). 03/2014; DOI:10.1645/13-366.1
The nervous system (NS) of the cestodes Diphyllobothrium dendriticum (Diphyllobothriidea) and Caryophyllaeus laticeps (Caryophyllidea) was investigated using immunocytochemistry. The GABA neurotransmitter was identified in the NS of both species; GABAergic neurons were detected in the main nerve cords (MC). GABA-like immunoreactive neurons were predominantly unipolar and exhibited more intensive immunoreactivity in the neurite than in the perikaryon. In C. laticeps, GABA-like immunoreactive somas are located in both the MCs and peripheral NS near the longitudinal muscles. The innervation of the body musculature was studied using a combination of antibodies against GABA, serotonin (5-HT), and FMRFamide and with complementary staining of F-actin. In both species, the location of GABAergic neurites is associated with all muscle layers, including the subtegumental, longitudinal, transverse and dorsoventral muscles. The cytomorphology of 5-HTergic motoneurons in the MCs of both species is described and differences in muscle innervation between D. dendriticum and C. laticeps are demonstrated. No evidence for co-localization of GABA with 5-HT or FMRFamide neurotransmitters at any particular neuron was found. Neuropiles in MCs and peripheral NS had separate sets of immunoreactive neurites. A functional role for GABA in muscle innervation is discussed.
Journal of Parasitology (Impact Factor: 1.32). 03/2014; DOI:10.1645/13-366.1
The nervous system (NS) of the cestodes Diphyllobothrium dendriticum (Diphyllobothriidea) and Caryophyllaeus laticeps (Caryophyllidea) was investigated using immunocytochemistry. The GABA neurotransmitter was identified in the NS of both species; GABAergic neurons were detected in the main nerve cords (MC). GABA-like immunoreactive neurons were predominantly unipolar and exhibited more intensive immunoreactivity in the neurite than in the perikaryon. In C. laticeps, GABA-like immunoreactive somas are located in both the MCs and peripheral NS near the longitudinal muscles. The innervation of the body musculature was studied using a combination of antibodies against GABA, serotonin (5-HT), and FMRFamide and with complementary staining of F-actin. In both species, the location of GABAergic neurites is associated with all muscle layers, including the subtegumental, longitudinal, transverse and dorsoventral muscles. The cytomorphology of 5-HTergic motoneurons in the MCs of both species is described and differences in muscle innervation between D. dendriticum and C. laticeps are demonstrated. No evidence for co-localization of GABA with 5-HT or FMRFamide neurotransmitters at any particular neuron was found. Neuropiles in MCs and peripheral NS had separate sets of immunoreactive neurites. A functional role for GABA in muscle innervation is discussed.
Research Interests:
The composition of leucocytes of the head kidney is studied in the crucian carps (Carassius auratus) either contaminated or uncontaminated with Digramma interrupta. The composition of leucocytes in the pronephros of the crucian carp from... more
The composition of leucocytes of the head kidney is studied in the crucian carps (Carassius auratus) either contaminated or uncontaminated with Digramma interrupta. The composition of leucocytes in the pronephros of the crucian carp from Lake Baikal basin has a lymphoid character. Compared to the crucian carp from the European part of Russia, in the fish from Baikal the granulocytopoetic processes are more pronounced. This is proved by the high content of young forms of granulocytes. In the fish infected with digramma, the immune suppression of proliferation of blasts and young forms of eosinophils was revealed. On the other hand, the inflammatory and humoral specific immune reactions are enhanced. Partial suppression of the immune response of C. auratus to invasion by D. interrupta facilitates development of the parasite.
Using immunofluorescence methods, for the first time distribution of the host immunity regulator – prostaglandin E2 in the organism of the tapeworm Diphyllobothrium dendriticum was revealed. PG E2-like immunoreactivity (IR) was revealed... more
Using immunofluorescence methods, for the first time distribution of the host immunity regulator – prostaglandin E2 in the organism of the tapeworm Diphyllobothrium dendriticum was revealed. PG E2-like immunoreactivity (IR) was revealed in large cells is situated in the zone of the cortical parenchyma and also in drop-shaped dilatations on the surface of tegument’s ectal cytoplasm. Overlapping areas of IR to PG E2 and to acetylated tubuline was revealed. PG E2-like-IR areas is situated within α-tubuline-IR areas and occupy the lesser space when compared with them. With respect to distribution and a number of morphological features, PG E2-like- and α-tubuline-IR elements conform to frontal glands, excreting secreta on the surface of the tegument through own specialized ducts. Therefore, the glands have immunomodulatory function, synthesizing and excreting prostaglandin Е2 into host tissues.
Marine foods represent a unique source of poly-unsaturated fatty acids (PUFA) of the omega-3 (n-3) family.Today it is generally accepted that fish oil is important in a healthy and balanced omnivorous human diet. This favorable health... more
Marine foods represent a unique source of poly-unsaturated fatty acids (PUFA) of the omega-3 (n-3) family.Today it is generally accepted that fish oil is important in a healthy and balanced omnivorous human diet. This favorable health perception of fish oil is however troubled by the high level of PUFA oxidation and low absorption in the gastro-intestinal tract. In this work we present and described various types of delivery systems which are used to improve PUFA and fish oil availability and oxidative stability.
Age dependent changes of the mesenteric lymphatic node of Baikal seal were studied. Microanatomical (relative area of structural components) and cytological characteristics of the mesenteric lymphatic node in adolescent (2 and 3 years)... more
Age dependent changes of the mesenteric lymphatic node of Baikal seal were studied. Microanatomical (relative area of structural components) and cytological characteristics of the mesenteric lymphatic node in adolescent (2 and 3 years) and adult (older than 4 years) seals were assessed. It was shown that the area of a sheath and trabeculas is considerably increased with age. Also the area occupied by nodules with light centre considerably decreased and the area taken by nodules without light centre increased with age. Decrease of the area of cerebral matter with age is significant. In the light centre of the lymphatic node the amount of blast forms of cells, the number of plasma cells and destructively changed cells increased with age. Density of cells in the light centre is higher in the adult animals than adolescent ones. Density of cells in medullar zone is higher in adults. The increase of reticular and destructively changed cells in paracortical zone with age were observed. The number of medium and small lymphocytes decreased. In the plasma cells’ group an increase in the number of unripe cells and a decrease in the number mature cells with age took place.
Research Interests:
It has investigated structural organization of mesenteric lymphatic nodes of baical nerpa and it has determined features of it’s age changes. It has discovered that relative square of connective tissue component increases, squares of... more
It has investigated structural organization of mesenteric lymphatic nodes of baical nerpa and it has determined features of it’s age changes. It has discovered that relative square of connective tissue component increases, squares of cortex and me-dullar decrease, parts of secondary lymph nodules and paracortical zone decrease in cortex in postnatal ontogeny. Cortex-medullar index has tendency to decreasing be-cause of age increasing. Discovered regularity point to that mesenteric lymphatic nodes of baical nerpa suffer involution and decreasing of it’s lymphopoiesis function take place in postnatal ontogeny. It is possible to explain decreasing of cortex-medullar index in age of from 1 month to 4 years by increasing of part of medullar, which is connected with motor function of nodes.