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Authors: Takeda, Kazuya | Tanabe, Shigeo | Koyama, Soichiro | Shomoto, Koji | Naoi, Yuki | Sakurai, Hiroaki | Kanada, Yoshikiyo

Article Type: Research Article

Abstract: BACKGROUND: The relationship between peak torque of the knee extensor muscles and gait speed was previously investigated in patients with chronic stroke, but whether the rate of force development (RFD), another indicator of muscle strength, affected gait speed remained unknown. OBJECTIVE: To clarify the relationships between the RFD of the knee extensor muscles over multiple time intervals and gait speed in patients with chronic stroke. METHODS: Twenty chronic stroke patients participated in this study. The RFD of affected and unaffected knee extensor muscles was measured. Time intervals of the RFD were set to 0–50 ms, 0–100 ms (100RFD), 0–200 ms (200RFD), and 0–300 ms …(300RFD). Gait speed was assessed at comfortable and maximum pace. Pearson correlation coefficient (r) was used to analyze the relationships between RFD and gait speeds. RESULTS: The RFD of affected knee extensor muscles over all intervals was moderately correlated with both comfortable and maximum gait speeds, whereas only limited combinations (100, 200 and 300RFD with maximum gait speed) were correlated on the unaffected side. CONCLUSIONS: The present results suggest that various factors related to the RFD of affected knee extensor muscles, such as neural drive and muscle size, affect gait speed. Show more

Keywords: Stroke, rate of force development, gait speed, correlation

DOI: 10.3233/NRE-182455

Citation: NeuroRehabilitation, vol. 43, no. 4, pp. 425-430, 2018

Authors: Hossain, Shahanara | Alim, Abdul | Takeda, Kazuya | Kaji, Hiroyuki | Shinoda, Tomotaka | Uéda, Kenji

Article Type: Research Article

Abstract: The NAC region of NACP/α-synuclein is a secondary component of Alzheimer's disease amyloid. α-Synuclein is a major component of Lewy bodies, a typical neuropathological feature of Parkinson's disease. However, the physiological role and deposition mechanisms of α-synuclein are unknown. Structural analyses of α-synuclein should provide a better understanding of its biochemical characteristics. We investigated the digestion of α-synuclein withα-chymotrypsin and cathepsin D, which are reported to be involved in amyloidogenesis, under various conditions in vitro. There are many putative cleavage sites for these enzymes in α-synuclein, including in the NAC region. However, most of the predicted sites remained undigested, and …the NAC region was found to be intact even after extensive digestion. This peculiar characteristic of α-synuclein may be relevant to the abnormal deposition of this molecule in α-synuclein-associated neurodegenerative diseases. Show more

DOI: 10.3233/JAD-2001-3608

Citation: Journal of Alzheimer's Disease, vol. 3, no. 6, pp. 577-584, 2001

Authors: Takeda, Kazuya | Tanabe, Shigeo | Koyama, Soichiro | Shomoto, Koji | Nagai, Tomoko | Naoi, Yuki | Ushiroyama, Kosuke | Saito, Yumi | Sakurai, Hiroaki | Kanada, Yoshikiyo

Article Type: Research Article

Abstract: INTRODUCTION: The rate of force development (RFD) is increased by high-load isometric resistance training (RT), whereas not by low-load isometric RT. However, it is unknown whether low-load isometric RT with neuromuscular electrical stimulation (NMES) can increase the RFD. PURPOSE: The aim of this study was to clarify the short-term effects of low-load isometric RT with NMES on the RFD. METHOD: Twenty-four healthy subjects randomly received both low-load RT of the hip abductor muscles with NMES and sham NMES condition. The RFD of the homonymous muscles was assessed at pre- and post-intervention. Time intervals of the RFD were set to 0–50 ms …(50RFD), 0–100 ms (100RFD), 0–200 ms (200RFD), and 0–300 ms (300RFD). Two-way repeated measures analysis of variance was used to analyze the effects of time and intervention. RESULT: Low-load RT with NMES significantly increased 50 and 100RFD compared to sham NMES condition. On the other hand, 200 and 300RFD were not changed by either condition. CONCLUSIONS: The significantly increased RFD might be related to the factors affecting each RFD interval. This finding might have an important clinical implication for developing a mechanism-based effective training. Show more

Keywords: Rate of force development, hip abductor muscles, neuromuscular electrical stimulation, low-load resistance training, healthy adults

DOI: 10.3233/PPR-190140

Citation: Physiotherapy Practice and Research, vol. 41, no. 1, pp. 3-9, 2020

Authors: Hirakawa, Yuichi | Koyama, Soichiro | Takeda, Kazuya | Iwai, Masanobu | Motoya, Ikuo | Sakurai, Hiroaki | Kanada, Yoshikiyo | Kawamura, Nobutoshi | Kawamura, Mami | Tanabe, Shigeo

Article Type: Case Report

Abstract: BACKGROUND: There are no reports regarding the long-term retention of effects of Lee Silverman Voice Treatment® BIG (LSVT® BIG) on improvements in quality of life (QOL) among patients with Parkinson’s disease (PD). OBJECTIVE: This study aimed to evaluate the short-term effect of LSVT® BIG on QOL improvement and its retention in a patient with PD. Motor symptoms, walking ability, and walking speed were evaluated as factors associated with QOL. METHODS: A 63-year-old woman who was diagnosed with PD received a 4-week LSVT® BIG program under the supervision of certified LSVT® BIG physical therapists. The participant’s disease severity was classified as …Hoehn and Yahr stage 2. The Parkinson’s Disease Questionnaire-39 (PDQ-39), Movement Disorder Society-sponsored Unified Parkinson’s Disease Rating Scale (MDS-UPDRS) part 3, timed up-and-go test (TUG), and 10 m walk test (10 MWT) were evaluated before, after, and 1-year after the intervention. RESULTS: The results indicated short-term improvements in the PDQ-39, MDS-UPDRS part 3, TUG, and 10 MWT which were retained for up to 1 year. CONCLUSIONS: This case report suggests the possibility of 1-year retention of improvements in QOL, motor symptoms, walking ability, and walking speed resulting from LSVT® BIG intervention in a patient with mild PD. Show more

Keywords: Parkinson’s disease, Lee Silverman Voice Treatment® BIG, long-term retention, case report

DOI: 10.3233/NRE-210129

Citation: NeuroRehabilitation, vol. 49, no. 3, pp. 501-509, 2021

Authors: Motoya, Ikuo | Tanabe, Shigeo | Hirakawa, Yuichi | Iwai, Masanobu | Takeda, Kazuya | Koyama, Soichiro | Kanada, Yoshikiyo | Kawamura, Nobutoshi | Kawamura, Mami | Sakurai, Hiroaki

Article Type: Research Article

Abstract: BACKGROUND: The COVID-19 pandemic has affected physical and occupational therapist education in various ways. OBJECTIVE: This study investigated whether the pandemic changed clinical placement, thus influencing the clinical competence of physical and occupational therapists at a hospital in Japan. METHODS: Eleven therapists (seven physical and four occupational) participated in this study in April 2021. Clinical placement teaching methods were surveyed using an online questionnaire. The Objective Structured Clinical Examination (OSCE), comprising attitudinal and technical items, was used to examine clinical competence. The results were calculated as the sum of the attitudinal and technical scores, and the ratios of these scores …to the full score were compared using a paired-sample Wilcoxon signed-rank test. RESULTS: During the pandemic, some schools implemented shortened practical clinical placements. The substituted classes included online-only classes and combined online and face-to-face classes. Regarding clinical competence, scores on the OSCE were mostly high. The median proportion of the total attitudinal score to the perfect score was 100% for all three items (range-of-motion test, muscle strength test, Stroke Impairment Assessment Set). The median proportion of the total technical score to the perfect score ranged from 56.7% to 76.7% for the three items. The ratio of the total attitudinal score to the full score was significantly higher than that of the total technical score to the full score (p = 0.001). CONCLUSIONS: Most clinical placements were canceled or partially administered through online learning during the pandemic. This decrease in clinical placements did not affect newly recruited physical and occupational therapists’ clinical competence. Show more

Keywords: Occupational therapy, physical therapy, continuing education, work, online learning, clinical practicum

DOI: 10.3233/WOR-230167

Citation: Work, vol. 78, no. 2, pp. 267-277, 2024

Authors: Alim, Muhammad Abdul | Ma, Qiu-Lan | Takeda, Kazuya | Aizawa, Takako | Matsubara, Mamoru | Nakamura, Minako | Asada, Akiko | Saito, Taro | xKaji, Mitsunobu | Yoshii, Mitsunobu | Hisanaga, Shinichi | Uéda, Kenji

Article Type: Research Article

Abstract: α-Synuclein is a major constituent of pathological intracellular inclusion bodies, a common feature of several neurodegenerative diseases. Two missense mutations in the α-synuclein gene have been identified in confirmed autosomal-dominant familial Parkinson's disease, which segregate with the illness. However, the physiological function of α-synuclein remains unknown. After biochemical investigations we have revealed tubulin to be an α-synuclein associated/binding protein. Here, we show that α-synuclein induces polymerization of purified tubulin into microtubules. Mutant forms of α-synuclein lose this potential. The binding site of α-synuclein to tubulin is identified, and co-localization of α-synuclein with microtubules is shown in cultured cells. To our …knowledge, this is the first demonstration of microtubule-polymerizing activity of α-synuclein. Now we can see a striking resemblance between α-synuclein and tau: both have the same physiological function and pathological features, making abnormal structures in diseased brains known as synucleinopathies and tauopathies. The discovery of a physiological role for α-synuclein may provide a new dimension in researches into the mechanisms of α-synuclein-associated neurodegenerative diseases. Show more

Keywords: lewy body, MAP, neurodegeneration, Parkinson, synuclein, tubulin

DOI: 10.3233/JAD-2004-6412

Citation: Journal of Alzheimer's Disease, vol. 6, no. 4, pp. 435-442, 2004

Authors: Shiota, Satomi | Takekawa, Hidenori | Matsumoto, Shin-ei | Takeda, Kazuya | Nurwidya, Fariz | Yoshioka, Yasuko | Takahashi, Fumiyuki | Hattori, Nobutaka | Tabira, Takeshi | Mochizuki, Hideki | Takahashi, Kazuhisa

Article Type: Research Article

Abstract: Previous studies have shown a high prevalence of obstructive sleep apnea (OSA) among patients with Alzheimer's disease (AD). However, it is poorly assessed whether chronic intermittent hypoxia (CIH), which is a characteristic of OSA, affects the pathophysiology of AD. We aimed to investigate the direct effect of intermittent hypoxia (IH) in pathophysiology of AD in vivo and in vitro. In vivo, 15 male triple transgenic AD mice were exposed to either CIH or normoxia (5% O2 and 21% O2 every 10 min, 8 h/day for 4 weeks). Amyloid-β (Aβ) profile, cognitive brain function, and brain pathology were evaluated. In vitro, …human neuroblastoma SH-SY5Y cells stably expressing wild-type amyloid-β protein precursor were exposed to either IH (8 cycles of 1% O2 for 10 min followed by 21% O2 for 20 min) or normoxia. The Aβ profile in the conditioned medium was analyzed. CIH significantly increased levels of Aβ42 but not Aβ40 in the brains of mice without the increase in hypoxia-inducible factor 1, alpha subunit (HIF-1α) expression. Furthermore, CIH significantly increased intracellular Aβ in the brain cortex. There were no significant changes in cognitive function. IH significantly increased levels of Aβ42 in the medium of SH-SY5Y cells without the increase in the HIF-1α expression. CIH directly and selectively increased levels of Aβ42 in the AD model. Our results suggest that OSA would aggravate AD. Early detection and intervention of OSA in AD may help to alleviate the progression of the disease. Show more

Keywords: Alzheimer's disease, amyloid-β peptide, hypoxia, obstructive sleep apnea

DOI: 10.3233/JAD-130419

Citation: Journal of Alzheimer's Disease, vol. 37, no. 2, pp. 325-333, 2013

Authors: Ma, Linqing | Ohyagi, Yasumasa | Miyoshi, Katsue | Sakae, Nobutaka | Motomura, Kyoko | Taniwaki, Takayuki | Furuya, Hirokazu | Takeda, Kazuya | Tabira, Takeshi | Kira, Jun-ichi

Article Type: Research Article

Abstract: Presenilin 1 (PS1) gene mutations are the major causes of early-onset familial Alzheimer's disease and are known to increase amyloid-β42 (Aβ42 ) production as well as to promote apoptosis. We have recently reported that intracellular Aβ42 activates p53 mRNA expression and promotes p53-dependent apoptosis. Here, we examined the p53 mRNA and protein levels in cells transfected with wild-type and I143T/G384A mutant PS1 genes. Although the baseline p53 mRNA levels remained unaltered, the p53 protein levels were significantly elevated in mutant PS1-transfected cells. Treatments with apoptosis-inducing agents induced significant elevation of the p53 protein but not p53 mRNA levels in mutant …PS1-transfected cells. Treatment with a β-secretase inhibitor and γ-secretase inhibitor decreased the intracellular Aβ levels in amyloid-β protein precursor (AβPP) and PS1-double transfected cells, and restrained upregulation of the p53 protein levels in the mutant PS1-transfected cells. Also, we found that proteasome activity was decreased in mutant PS1-transfected cells compared to wild-type PS1-transfected cells. Proteasome activity was further decreased in AβPP/PS1-double transfected cells. Taken together, p53-dependent apoptosis upregulated by the I143T/G384A mutant PS1 gene may be associated, at least in part, with intracellular Aβ and proteasome impairment. Show more

Keywords: Alzheimer's disease, p53, presenilin, proteasome, β-secretase, γ-secretase

DOI: 10.3233/JAD-2009-0990

Citation: Journal of Alzheimer's Disease, vol. 16, no. 3, pp. 565-575, 2009