Your search for: 'author:("Zhu, Jing")' has returned 11 results. (0.021s) Save search

Authors: Chen, Jing | Wang, Huan | Zhu, Jing

Article Type: Research Article

Abstract: BACKGROUND: Hypertensive disorders during pregnancy pose significant risks to both maternal and fetal health, necessitating safe and effective therapeutic interventions. OBJECTIVE: This study aimed to investigate the potential of an extract derived from Falcaria vulgaris (FV), loaded with exosomes to form the Exo/FV complex, as a novel therapeutic agent for the management of hypertension in pregnant mice: antioxidants, antimicrobials, and phenolic compounds present in FV lower blood pressure. METHODS: The isolation of exosomes was done by ultracentrifugation methods and the FV was loaded into the exosomes by electroporation method. RESULTS: The Exo/FV was found to be spherical with diameter ranges …from 20 to 30 nm and they were tested for biocompatibility in NHI 3T3 cell lines and found to be effective. This research investigated in vivo hypertension in mice induced by L-NAME and treated with FV and Exo/FV and found that AChE and MAO determine mice’s redox state tends to reduce blood pressure. Increased non-protein thiol (NP-SH) and decreased lipid peroxidation were also found, and PDE-5, ACE, Arginase, and MDA activity has also been tested. CONCLUSION: This analysis showed that Exo/FV effectively treated hypertension during pregnancy. Show more

Keywords: Hypertension, Falcaria vlugaris, exosomes, In vivo, Exo/FV

DOI: 10.3233/BME-240053

Citation: Bio-Medical Materials and Engineering, vol. Pre-press, no. Pre-press, pp. 1-18, 2024

Authors: ZHU, Jing-ping | HU, Yong-you | LIANG, Hui-qiang

Article Type: Research Article

Abstract: The feasibility of the nitrous organic wastewater treated was studied in seven anaerobic sequencing batch reactors (ASBRs) (0^# –6^# ) which had been run under stable anaerobic ammonium oxidation (Anammox). By means of monitoring and data analysis of COD, NH_4^+ -N, NO_2^- -N, NO_3^- -N and pH, and of microbial test, the results revealed that the optimal Anammox performance was achieved from 2^# reactor in which COD/NH_4^+ -N was 1.65, Anammox bacteria and denitrification bacteria could coexist, and Anammox reaction and denitrification reaction could occur simultaneously in the reactors. The ratio of NH_4^+ -N consumed: NO_2^- -N consumed: NO_3^- -N …produced was 1:1.38:0.19 in 0^# reactor which was not added glucose in the wastewater. When different ratio of COD and NH_4^+ -N was fed for the reactors, the ratio of NO_2^- -N consumed: NH_4^+ -N consumed was in the range of 1.51–2.29 and the ratio of NO_3^-<FORMULA>-N produced: NH<FORMULA>_4^+ -N consumed in the range of 0–0.05. Show more

Keywords: anaerobic ammonium oxidation (Anammox), anaerobic sequencing batch reactor (ASBR), biological denitrification, denitrification

Citation: Journal of Environmental Sciences, vol. 18, no. 2, pp. 227-231, 2006

Authors: Yao, Yi | Liu, Jialuo | Zhu, Jing | Gu, Xiaodong

Article Type: Research Article

Abstract: Integer vulnerability is a common and hidden problem of software security. Even mature software written by experienced programmers cannot avoid integer vulnerability. Therefore, it is very important for testers to detect the bugs. However, testing the bugs will face the test oracle problem. As far as we know, the metamorphic testing can effectively alleviate the test oracle problem. In this paper, a method of combinational metamorphic relations (CMR) is proposed, which solves the test oracle problem and is more effective than metamorphic relation for Integer vulnerability detection. A case study results shows that the combinational metamorphic relations are more effective …in bugs-detection than the single metamorphic relations. Show more

Keywords: Metamorphic testing, oracle problem, combinational metamorphic relation

DOI: 10.3233/JCM-194105

Citation: Journal of Computational Methods in Sciences and Engineering, vol. 20, no. 4, pp. 1053-1061, 2020

Authors: Zhu, Jing-E | Chen, Yun-Chao | Yu, Song-Yuan | Xu, Hui-Xiong

Article Type: Case Report

Abstract: Schwannoma is a benign tumor that originates from Schwann cells in the nerve sheathing of cranial, other peripheral, or autonomic nerves. Patients often present with painless mass as the chief complaint. The main symptoms of this tumor are related to its size and specific nerve origin. At present, the pretreatment diagnosis is mainly made by ultrasound, CT, MR, or biopsy, and the main treatment is surgical resection. We reported a new treatment method for cervical schwannoma in a 65-year-old woman with a history of non-small cell lung cancer (NSCLC). When the patient’s neck mass was initially found with hoarseness and …severe cough, it was considered as cervical lymph node metastasis of lung cancer due to her medical history. And she was diagnosed with schwannoma by core-needle biopsy after chemotherapy failed and the tumor shrank after the radiotherapy with no improvement of the clinical symptoms. After considering the physical condition, the patients were treated in our department for minimal invasiveness treatment. The patient was definitively diagnosed with cervical vagus schwannoma and was treated with ultrasound-guided microwave ablation of schwannoma under general anesthesia with systematic evaluation and improved preoperative examination. Her condition was stable, and the symptoms of severe cough disappeared after anesthesia resuscitation and the ablation. The tumor continued to shrink after the operation with no recurrence of cough symptoms. Ultrasound-guided percutaneous microwave ablation (MWA) for cervical vagus schwannomas might be a minimally invasive, effective, and relatively safe alternative to conventional treatment for those patients with severe symptoms. Show more

Keywords: Extracranial schwannoma, cervical vagus nerve, microwave ablation

DOI: 10.3233/CH-211301

Citation: Clinical Hemorheology and Microcirculation, vol. 80, no. 4, pp. 437-446, 2022

Authors: Zhu, Jing | Kang, Jing | Li, Xiaochen | Wang, Mengmei | Shang, Min | Luo, Yuchuan | Xiong, Mengqing | Hu, Ke

Article Type: Research Article

Abstract: BACKGROUND AND AIM: Both chronic intermittent hypoxia (CIH) and chronic continuous hypoxia (CCH) are risk factors for cardiovascular disease, which are associated with cardiac systolic function and associated with dysfunction of endothelia and coagulation-fibrinolysis system in the vasculature. However, the different effects of these two hypoxic models are not fully understood. In our study, we systemically compared the effects of CIH and CCH on cardiac function and related factor levels in serum using rat model. METHODS: Forty-five male Sprague-Dawley rats were randomly divided into the normoxia control (NC), CIH and CCH groups. The rat CIH and CCH models were established, …then the blood and tissue samples were collected to analyze the function of endothelium and the coagulation-fibrinolysis system. Also, the ultrasound cardiogram was performed to directly assess myocardial contractility. RESULTS: Both CIH and CCH significantly decreased the NO, eNOS, P-eNOS and AT-III levels in the rat serum but significantly increased the levels of ET-1, vWF, COX-2, NF-κ B, FIB, FVIII and PAI-1 in the rat serum (P  < 0.05). The expression of ET-1, VWF and ICAM-1 in CIH group were higher than CCH group (P  < 0.05), however, the expression of CD62p was increased in CCH group but not in CIH group. The expression of t-PA in CIH group were lower than CCH group (P  < 0.05), but there were no significant differences in CCH group and NC group (P  > 0.05). Using transmission electron microscope, we found that the mitochondrial ultrastructure of thoracic aorta endothelial cells in CIH and CCH group were damaged. Moreover, the myocardial contractility in CIH and CCH group were significantly decreased compared with NC group. CONCLUSION: Our results suggested that CIH and CCH could cause endothelial dysfunction, dysfunction of the coagulation-fibrinolysis system and decreasing of myocardial contractility. Compared with CCH, CIH has greater effect on vasoconstriction and adhesion of vascular endothelial cells, and stronger procoagulant effect. Show more

Keywords: Chronic intermittent hypoxia, chronic continuous hypoxia, vascular endothelial function, myocardial contractility

DOI: 10.3233/CH-190706

Citation: Clinical Hemorheology and Microcirculation, vol. 74, no. 4, pp. 417-427, 2020

Authors: Zhu, Jing-E. | Zhang, Hui-Li | Yu, Song-Yuan | Xu, Hui-Xiong

Article Type: Research Article

Abstract: Hyperthyroidism is a common disease mainly manifested by hyperexcitability of multiple systems and hypermetabolism. Currently, antithyroid drugs (ATDs), radioiodine therapy (RIT), and surgery are mainly used in the clinical treatment for primary hyperthyroidism. We reported a case of a 28-year-old female who received a novel treatment for primary hyperthyroidism. This patient had poor control of thyroid function while taking ATD, and her oral Methimazole (MMI) dose varied repeatedly between 20 mg qd and 15 mg qd, failing to maintain a stable status. To minimize the possible complication and to achieve drug reduction or withdrawal, she refused RIT and surgery and showed up …in our department. The patient, diagnosed with Graves’ disease (GD) and met the surgical indication after systematic clinical evaluation, was subject to ultrasound-guided percutaneous microwave ablation (MWA) of the partial thyroid gland with continuous oral administration of 20 mg qd MMI. The post-ablation condition was stable and the patient was discharged 2 days after the operation. Thyroid ultrasound and serum thyroid function test were examined regularly after ablation and the MMI dosage was gradually reduced according to the results of the biochemical examination. Five weeks after the operation, the patient completely discontinued the medication. Ultrasound-guided percutaneous microwave ablation is minimally invasive, safe, and effective, and has potential to be an alternative treatment besides the 3 classical treatments of hyperthyroidism. Show more

Keywords: Contrasted-enhanced ultrasound, hyperthyroidism, microwave ablation

DOI: 10.3233/CH-211180

Citation: Clinical Hemorheology and Microcirculation, vol. 79, no. 3, pp. 435-444, 2021

Authors: Li, Jia-Xin | Li, Xiao-Long | Zhu, Jing-E | Zhang, Hui-Li | Yu, Song-Yuan | Xu, Hui-Xiong

Article Type: Research Article

Abstract: INTRODUCTION: Abdominal wall endometriosis (AWE) is a relatively uncommon condition associated with diagnostic and therapeutic difficulties among all the extra pelvic endometriosis. The main therapies include surgery and oral contraceptive administration. Percutaneous cryoablation and high intensity focused ultrasound (HIFU) are also proven to be valid alternatives. Microwave ablation (MWA) as one of the thermal ablation methods has not been applied in the treatment of AWE yet. Herein the feasibility of ultrasound (US) –guided MWA for AWE was explored and treatment response evaluation was carried out using contrast-enhanced imaging. METHODS: Three consecutive patients who underwent US-guided MWA for AWE with typical …symptoms were included in this retrospective study. US, magnetic resonance imaging (MRI), laboratory tests, and US-guided core-needle biopsy were conducted for pre-treatment assessment and ruling out malignancy. The interventional procedure was carried out under local anesthesia with MWA and the output power was 60w. Post-treatment contrast-enhanced ultrasound (CEUS) was performed to evaluate the instant treatment response. The follow-up intervals were 1 month, 6 months, and 12 months after treatment. The clinical symptoms and condition of AWE lesions were recorded in each follow-up. RESULTS: The MWA procedure was completed in all the patients with no blood perfusion inside each lesion by instant CEUS after treatment. The mean ablation time was 687 seconds (s) for a single patient (ranged from 660s to 742s). Clinical symptoms were relieved evidently at the end of the follow-up. The pain according to the visual analogue scale (VAS) decreased from 4–6 before treatment to 0–2 after treatment. Mild to moderate complications included slightly abdominal pain and fat liquefaction occurred. In terms of technical outcomes, the volume of all six lesions reduced in different degrees at the end of follow-up (ranged: 16.6% to 100%). CONCLUSION: US-guided MWA may be a feasible and promising approach for symptomatic AWE. Show more

Keywords: Contrast-enhanced imaging, abdominal wall endometriosis, microwave ablation

DOI: 10.3233/CH-211287

Citation: Clinical Hemorheology and Microcirculation, vol. 82, no. 1, pp. 63-73, 2022

Authors: Mao, Zhijun | Wang, Zhen | Zhang, Shiping | Pu, Yansong | Wang, Jianhua | Zhang, Tao | Long, Yanbin | Liu, Yi | Ma, Yu | Zhu, Jing

Article Type: Research Article

Abstract: BACKGROUND: Low-density lipoprotein receptor-related protein 4 (LRP4) has been reported to be implicated in multiple types of cancers. However, the significance of LRP4 in gastric cancer (GC) remains poorly elucidated. Therefore, it’s urgent to investigate the importance and underlying mechanisms of LRP4 in GC. OBJECTIVE: To investigate the clinical roles of LRP4 in GC. METHODS: The LRP4 mRNA and miR-140-5p was measured by qRT-PCR. The protein expression was determined Western blot. Kaplan-Meier survival curves and Cox proportional hazard regression models were performed to evaluate prognosis. RESULTS: We demonstrated that LRP4 mRNA and protein was up-regulated in GC tissues for the …first time. Its high expression was significantly correlated with malignant clinical features including TNM stage and lymph-node metastasis and poor prognosis for GC patients. LRP4 promotes migration, invasion and epithelial-mesenchymal transition (EMT) progress of GC cells. Mechanically, LRP4 regulated PI3K/AKT in GC cells. AKT inhibitors reversed the effects of LRP4. Finally, LRP4 was regulated by miR-140-5p in GC. CONCLUSIONS: Our findings showed that LRP4 has an important function in GC progression and promotes GC migration, invasion and EMT by regulating PI3K/AKT under regulation of miR-140-5p, providing a potential therapeutic target for GC. Show more

Keywords: Gastric cancer, LRP4, miR-140-5p, EMT, PI3K/AKT

DOI: 10.3233/CBM-190571

Citation: Cancer Biomarkers, vol. 29, no. 2, pp. 245-253, 2020

Authors: Zhu, Jing | Ji, Xin | Shi, Ruirui | He, Tianqi | Chen, Su-ying | Cong, Ruochen | He, Bosheng | Liu, Su | Xu, Hui | Gu, Jin-hua

Article Type: Research Article

Abstract: Background: At least one-third of Alzheimer’s disease (AD) patients have cerebrovascular abnormalities, micro- and macro-infarctions, and ischemic white matter alterations. Stroke prognosis impacts AD development due to vascular disease. Hyperglycemia can readily produce vascular lesions and atherosclerosis, increasing the risk of cerebral ischemia. Our previous research has demonstrated that protein O-GlcNAcylation, a dynamic and reversible post-translational modification, provides protection against ischemic stroke. However, the role of O-GlcNAcylation in the exacerbation of cerebral ischemia injury due to hyperglycemia remains to be elucidated. Objective: In this study, we explored the role and underlying mechanism of protein O-GlcNAcylation in the exacerbation of cerebral …ischemia injury caused by hyperglycemia. Methods: High glucose-cultured brain microvascular endothelial (bEnd3) cells were injured by oxygen-glucose deprivation. Cell viability was used as the assay result. Stroke outcomes and hemorrhagic transformation incidence were assessed in mice after middle cerebral artery occlusion under high glucose and streptozotocin-induced hyperglycemic conditions. Western blot estimated that O-GlcNAcylation influenced apoptosis levels in vitro and in vivo . Results: In in vitro analyses showed that Thiamet-G induces upregulation of protein O-GlcNAcylation, which attenuates oxygen-glucose deprivation/R-induce injury in bEnd3 cells cultured under normal glucose conditions, while aggravated it under high glucose conditions. In in vivo analyses, Thiamet-G exacerbated cerebral ischemic injury and induced hemorrhagic transformation, accompanied by increased apoptosis. While blocking protein O-GlcNAcylation with 6-diazo-5-oxo-L-norleucine alleviated cerebral injury of ischemic stroke in different hyperglycemic mice. Conclusion: Overall, our study highlights the crucial role of O-GlcNAcylation in exacerbating cerebral ischemia injury under conditions of hyperglycemia. O-GlcNAcylation could potentially serve as a therapeutic target for ischemic stroke associated with AD. Show more

Keywords: Alzheimer’s disease, apoptosis, cerebral ischemia-reperfusion, hemorrhagic transformation, hyperglycemia, O-GlcNAcylation

DOI: 10.3233/JAD-230264

Citation: Journal of Alzheimer's Disease, vol. 94, no. 2, pp. 651-668, 2023

Authors: Zhu, Jing-E. | Li, Jia-Xin | Zhang, Hui-Li | Li, Xiao-Long | Sun, Li-Ping | Yu, Song-Yuan | Xu, Hui-Xiong

Article Type: Research Article

Abstract: Biliary cystadenocarcinoma (BCAC) is an extremely rare intrahepatic cystic tumor. Patients usually present with nonspecific symptoms such as abdominal pain, abdominal distention, and abdominal mass. This tumor occurs most commonly in the left hemiliver and is thought to mainly develop from a benign biliary cystadenoma (BCA). At present, the disease is mainly diagnosed by ultrasound, CT, MR, and other imaging methods, and the main treatment is radical surgical resection. We reported a 75-year-old female with an unresectable huge BCAC (i.e., 161×145×122 mm in three orthogonal directions) and poor general condition (40 in Karnofsky Performance Status, KPS) who received sequential thermal ablation …(i.e., cryoablation and microwave ablation) in combination with sclerotherapy using lauromacrogol. The diagnosis of intrahepatic BCAC was confirmed pathologically. Preablation grayscale US showed the BCAC with a clear boundary, regular shape, and cystic-solid mixed echogenicity, which appeared as a huge multilocular cystic lesions with thick internal sepatations. Preablation contrast-enhanced ultrasound (CEUS) showed honeycomb-like hyper-enhancement of the thick internal sepatations and cystic wall in the arterial and portal phase, and sustained enhancement of the thick internal sepatations and cystic wall in the late phase. 6-month postablation CEUS showed non-enhancement in most parts of the lesion in the arterial phase and 6-month postablation MRI showed the volume reduction ratio (VRR) was about 70%. The abdominal pain and abdominal distension were relieved remarkably, and her quality of life was greatly improved (70 in KPS). In conclusion, sequential thermal ablation in combination with sclerotherapy provides a successful translative therapy for this unresectable huge BCAC with a poor general condition, which makes subsequent curative surgery or ablation possible. Show more

Keywords: Biliary cystadenocarcinoma, microwave ablation, cryoablation, sclerotherapy

DOI: 10.3233/CH-221466

Citation: Clinical Hemorheology and Microcirculation, vol. 82, no. 1, pp. 95-105, 2022