I am an old man in search of a mechanism for perception and how it creates bliss. I may not see answers to these questions in my brief future but I know that I will be looking for them until ...
Growth of the yeast Brettanomyces/Dekkera in wine can drastically alter the aroma characteristics... more Growth of the yeast Brettanomyces/Dekkera in wine can drastically alter the aroma characteristics to the point where all varietal and regional flavor characteristics are overwhelmed by the flavors produced by these yeasts. To avoid spoilage it is important to know more about the aromas formed by these yeasts and be able to detect the changes in aroma early so that further growth of the yeast and further aroma modification can be avoided. Two groups of commercial wines with suspected "Brett" character were evaluated by two trained panels of judges. The first group included four Cabernet Sauvignon and two Pinot Noir wines; the second included four Cabernet Sauvignon wines. All were evaluated by sensory descriptive analysis and GC/MS 4-ethyl phenol analysis. Characteristic "Brett" aromas such as plastic, burnt plastic, Band-aid (TM), cow manure, barnyard, and horse sweat were summarized by the first group of tasters as 'plastic.' For the second group 'plastic' included only pastic, burnt plastic, and Band-aid (TM) odors. Dry manure and sweaty/animal were separate descriptors. In both groups, the wines were differentiated by univariate analysis of variance (ANOVA) and multivariate discriminant analysis (DA) by two descriptors: plastic and fruity. The greatest fruit character and lowest plastic scores defined the younger Cabernets, and the opposite was true for the older wines. There was little difference between the two Pinot Noirs for either descriptor. The a priori "Brett" observations from the winemakers proved to be a consistent predictor of Brett character for all wines. The observations also agreed with the 4-ethyl phenol concentrations and the post-hoc plastic mean scores from the ANOVA analyses. The "strong Brett" wines were the older vintage wines with higher 4-ethyl phenol concentrations, higher plastic and lower fruity mean scores. Wines with "maybe some" and "no Brett" had the lower 4-ethyl phenol concentrations and more fruit with less plastic scores. This investigation shows that aroma modifications by Brettanomyces yeasts can be reliably detected and quantified with trained tasters. Further investigations into the chemical basis of the 'Brett' aromas will allow us to use chemical indicators to detect activity of these yeasts early.
The Brettanomyces / Dekkera yeasts can be found in fermenting must and in wine. Typically they gr... more The Brettanomyces / Dekkera yeasts can be found in fermenting must and in wine. Typically they grow after alcoholic and malolactic fermentation during storage of wine in tank, barrel, or bottle. They contribute characteristic bretty flavors which are described as smoky, barnyard, plastic, burnt plastic, vinyl, Bandaid, and creosote. They can also contribute a metallic bitterness. Compounds which are responsible for brett flavor in wine include 4-ethyl phenol, 4-ethyl guaiacol, isovaleric acid, and unidentified burnt plastic compound. Descriptive sensory evaluations show an inverse relationship between fruity and bretty flavor perception. The brett aromas in some wines are considered a positive attribute, especially when present at low concentration. Often these flavors are considered a defect. The wine's varietal and regional flavor characteristics might be completely masked by these flavors and the wine can be unpleasantly bitter. 4-ethyl phenol is used by some wineries as an indicator compound for the activity of Brettanomyces. Yet some wines having a strong brett flavors do contain none or very little 4-ethyl phenol. Preliminary studies show that 4-ethyl phenol is formed all through growth of Brettanomyces. Thus this compound can be used to confirm the presence of bretty flavors. The search for indicator compounds formed during early stages of growth of B. continues. The Brettanomyces yeasts can produce the characteristic brett flavors when growing at low cell density of several hundred to several thousand cells per mL. The fact that these yeasts are often present at low cell numbers and that they are slow growing makes detection difficult. Genetic analyses of wine isolates have shown that only strains of B. bruxellensis grow in wine. Semi-selective plating is performed prior to genetic analysis on media including cycloheximide. The development of DNA based probes has made it possible to reliably identify the yeast once it is plated on a nutrient agar plate. Further developments of specific probes and of probe application techniques will improve the specificity and speed of detection.
In presenting the material in this chapter, four fundamental propositions are made. These are: (1... more In presenting the material in this chapter, four fundamental propositions are made. These are: (1) Sucrose (sugar) tastes sweet. (2) Quinine tastes bitter. (3) Sodium chloride tastes salty 1. (4) Hydrochloric acid tastes sour.
... IN RELATION TO PROCESSING AND VARIETY CY Lee, WB Robinson, JP Van Buren, TE Acree, and GS Sto... more ... IN RELATION TO PROCESSING AND VARIETY CY Lee, WB Robinson, JP Van Buren, TE Acree, and GS Stoewsand ... Wines prepared from the vinifera grapes 'Pinot noir' or 'Riesling' contained low amounts of methanol and were more uniform year to year. ... 'Vincent' 19 141 ...
Page 1. CONCORD WINE COMPOSITION AS AFFECTED AND PROCESSING TECHNIQUE BY MATURITY Richard R. Nels... more Page 1. CONCORD WINE COMPOSITION AS AFFECTED AND PROCESSING TECHNIQUE BY MATURITY Richard R. Nelson and Terry E. Acree Respectively Graduate Research Assistant and Associate Professor of Biochemistry ...
... IN RELATION TO PROCESSING AND VARIETY CY Lee, WB Robinson, JP Van Buren, TE Acree, and GS Sto... more ... IN RELATION TO PROCESSING AND VARIETY CY Lee, WB Robinson, JP Van Buren, TE Acree, and GS Stoewsand ... Wines prepared from the vinifera grapes 'Pinot noir' or 'Riesling' contained low amounts of methanol and were more uniform year to year. ... 'Vincent' 19 141 ...
Diacetyl, a flavor compound having a distinct buttery character, accumulates during alcoholic and... more Diacetyl, a flavor compound having a distinct buttery character, accumulates during alcoholic and malolactic fermentation (MLF) of wine. In an effort to investigate the occurrence of this compound in US Chardonnay wines, 41 wines from 36 wineries were analyzed for their diacetyl ...
Masking unpleasant odors with high levels of pleasant-smelling odorants is an ancient practice th... more Masking unpleasant odors with high levels of pleasant-smelling odorants is an ancient practice that has evolved into many enterprises, from perfumery to consumer products. However, effective odor masking turns out to be idiosyncratic and impermanent. Here, we used Sniff Olfactometry (SO)(Rochelle et al., 2017; Wyckoff & Acree, 2017) to investigate the psychophysics of masking during 70ms-stimulations with mixtures of the mal-odorantiso-valeric Acid (IVA) and different masking agents. IVA is a component of human sweat that can dominate its smell, and is often described in unpleasant terms, e.g., “gym locker”, “smelly feet”, “dirty clothes”, etc. Conventionally, high concentrations of positive smelling odorants are used to reduce the unpleasantness of IVA in clothing or environments contaminated with IVA. To investigate the masking effects of sub-threshold levels of masking agents (neohivernal, geraniol, florhydral, decanal,iso-longifolanone, methyliso-eugenol, ands-limonene) on IVA, ...
Research Note Incubation of plant material with potassium metabisulfite was found to inhibit fung... more Research Note Incubation of plant material with potassium metabisulfite was found to inhibit fungal infections of explants from grapevines. Grapevine tissue of Vitis labruscana cv. Concord was incubated with sulfite pads containing 0.4 g of potassium metabilsufite for one and two days prior to culturing and evaluated against a control that had been surface sterilized with 0.5 % NaOCI and 70 % ethanol after one week for losses due to microbial contamination. Sulfite fumigation of plant material reduced the incidence of mold infection, particularly in tissue cultures developed from fruit explants which had reductions in contamination as high as 10 fold. Continued attempts to isolate contaminants from cultures intitiated from these explants showed no signs of infection.
Growth of the yeast Brettanomyces/Dekkera in wine can drastically alter the aroma characteristics... more Growth of the yeast Brettanomyces/Dekkera in wine can drastically alter the aroma characteristics to the point where all varietal and regional flavor characteristics are overwhelmed by the flavors produced by these yeasts. To avoid spoilage it is important to know more about the aromas formed by these yeasts and be able to detect the changes in aroma early so that further growth of the yeast and further aroma modification can be avoided. Two groups of commercial wines with suspected "Brett" character were evaluated by two trained panels of judges. The first group included four Cabernet Sauvignon and two Pinot Noir wines; the second included four Cabernet Sauvignon wines. All were evaluated by sensory descriptive analysis and GC/MS 4-ethyl phenol analysis. Characteristic "Brett" aromas such as plastic, burnt plastic, Band-aid (TM), cow manure, barnyard, and horse sweat were summarized by the first group of tasters as 'plastic.' For the second group 'pl...
... Megazyme Total Starch Assay (Amyloglucosidase/a-Amylase Method) Alternate Protocol: Enzymatic... more ... Megazyme Total Starch Assay (Amyloglucosidase/a-Amylase Method) Alternate Protocol: Enzymatic Quantitation of Total Starch (Holm Method) Reagents ... Acree, Eric A. Decker, Michael H. Penner, David S. Reid, Steven J. Schwartz, Charles F. Shoemaker, Denise Smith, Peter ...
Growth of the yeast Brettanomyces/Dekkera in wine can drastically alter the aroma characteristics... more Growth of the yeast Brettanomyces/Dekkera in wine can drastically alter the aroma characteristics to the point where all varietal and regional flavor characteristics are overwhelmed by the flavors produced by these yeasts. To avoid spoilage it is important to know more about the aromas formed by these yeasts and be able to detect the changes in aroma early so that further growth of the yeast and further aroma modification can be avoided. Two groups of commercial wines with suspected "Brett" character were evaluated by two trained panels of judges. The first group included four Cabernet Sauvignon and two Pinot Noir wines; the second included four Cabernet Sauvignon wines. All were evaluated by sensory descriptive analysis and GC/MS 4-ethyl phenol analysis. Characteristic "Brett" aromas such as plastic, burnt plastic, Band-aid (TM), cow manure, barnyard, and horse sweat were summarized by the first group of tasters as 'plastic.' For the second group 'plastic' included only pastic, burnt plastic, and Band-aid (TM) odors. Dry manure and sweaty/animal were separate descriptors. In both groups, the wines were differentiated by univariate analysis of variance (ANOVA) and multivariate discriminant analysis (DA) by two descriptors: plastic and fruity. The greatest fruit character and lowest plastic scores defined the younger Cabernets, and the opposite was true for the older wines. There was little difference between the two Pinot Noirs for either descriptor. The a priori "Brett" observations from the winemakers proved to be a consistent predictor of Brett character for all wines. The observations also agreed with the 4-ethyl phenol concentrations and the post-hoc plastic mean scores from the ANOVA analyses. The "strong Brett" wines were the older vintage wines with higher 4-ethyl phenol concentrations, higher plastic and lower fruity mean scores. Wines with "maybe some" and "no Brett" had the lower 4-ethyl phenol concentrations and more fruit with less plastic scores. This investigation shows that aroma modifications by Brettanomyces yeasts can be reliably detected and quantified with trained tasters. Further investigations into the chemical basis of the 'Brett' aromas will allow us to use chemical indicators to detect activity of these yeasts early.
The Brettanomyces / Dekkera yeasts can be found in fermenting must and in wine. Typically they gr... more The Brettanomyces / Dekkera yeasts can be found in fermenting must and in wine. Typically they grow after alcoholic and malolactic fermentation during storage of wine in tank, barrel, or bottle. They contribute characteristic bretty flavors which are described as smoky, barnyard, plastic, burnt plastic, vinyl, Bandaid, and creosote. They can also contribute a metallic bitterness. Compounds which are responsible for brett flavor in wine include 4-ethyl phenol, 4-ethyl guaiacol, isovaleric acid, and unidentified burnt plastic compound. Descriptive sensory evaluations show an inverse relationship between fruity and bretty flavor perception. The brett aromas in some wines are considered a positive attribute, especially when present at low concentration. Often these flavors are considered a defect. The wine's varietal and regional flavor characteristics might be completely masked by these flavors and the wine can be unpleasantly bitter. 4-ethyl phenol is used by some wineries as an indicator compound for the activity of Brettanomyces. Yet some wines having a strong brett flavors do contain none or very little 4-ethyl phenol. Preliminary studies show that 4-ethyl phenol is formed all through growth of Brettanomyces. Thus this compound can be used to confirm the presence of bretty flavors. The search for indicator compounds formed during early stages of growth of B. continues. The Brettanomyces yeasts can produce the characteristic brett flavors when growing at low cell density of several hundred to several thousand cells per mL. The fact that these yeasts are often present at low cell numbers and that they are slow growing makes detection difficult. Genetic analyses of wine isolates have shown that only strains of B. bruxellensis grow in wine. Semi-selective plating is performed prior to genetic analysis on media including cycloheximide. The development of DNA based probes has made it possible to reliably identify the yeast once it is plated on a nutrient agar plate. Further developments of specific probes and of probe application techniques will improve the specificity and speed of detection.
In presenting the material in this chapter, four fundamental propositions are made. These are: (1... more In presenting the material in this chapter, four fundamental propositions are made. These are: (1) Sucrose (sugar) tastes sweet. (2) Quinine tastes bitter. (3) Sodium chloride tastes salty 1. (4) Hydrochloric acid tastes sour.
... IN RELATION TO PROCESSING AND VARIETY CY Lee, WB Robinson, JP Van Buren, TE Acree, and GS Sto... more ... IN RELATION TO PROCESSING AND VARIETY CY Lee, WB Robinson, JP Van Buren, TE Acree, and GS Stoewsand ... Wines prepared from the vinifera grapes 'Pinot noir' or 'Riesling' contained low amounts of methanol and were more uniform year to year. ... 'Vincent' 19 141 ...
Page 1. CONCORD WINE COMPOSITION AS AFFECTED AND PROCESSING TECHNIQUE BY MATURITY Richard R. Nels... more Page 1. CONCORD WINE COMPOSITION AS AFFECTED AND PROCESSING TECHNIQUE BY MATURITY Richard R. Nelson and Terry E. Acree Respectively Graduate Research Assistant and Associate Professor of Biochemistry ...
... IN RELATION TO PROCESSING AND VARIETY CY Lee, WB Robinson, JP Van Buren, TE Acree, and GS Sto... more ... IN RELATION TO PROCESSING AND VARIETY CY Lee, WB Robinson, JP Van Buren, TE Acree, and GS Stoewsand ... Wines prepared from the vinifera grapes 'Pinot noir' or 'Riesling' contained low amounts of methanol and were more uniform year to year. ... 'Vincent' 19 141 ...
Diacetyl, a flavor compound having a distinct buttery character, accumulates during alcoholic and... more Diacetyl, a flavor compound having a distinct buttery character, accumulates during alcoholic and malolactic fermentation (MLF) of wine. In an effort to investigate the occurrence of this compound in US Chardonnay wines, 41 wines from 36 wineries were analyzed for their diacetyl ...
Masking unpleasant odors with high levels of pleasant-smelling odorants is an ancient practice th... more Masking unpleasant odors with high levels of pleasant-smelling odorants is an ancient practice that has evolved into many enterprises, from perfumery to consumer products. However, effective odor masking turns out to be idiosyncratic and impermanent. Here, we used Sniff Olfactometry (SO)(Rochelle et al., 2017; Wyckoff & Acree, 2017) to investigate the psychophysics of masking during 70ms-stimulations with mixtures of the mal-odorantiso-valeric Acid (IVA) and different masking agents. IVA is a component of human sweat that can dominate its smell, and is often described in unpleasant terms, e.g., “gym locker”, “smelly feet”, “dirty clothes”, etc. Conventionally, high concentrations of positive smelling odorants are used to reduce the unpleasantness of IVA in clothing or environments contaminated with IVA. To investigate the masking effects of sub-threshold levels of masking agents (neohivernal, geraniol, florhydral, decanal,iso-longifolanone, methyliso-eugenol, ands-limonene) on IVA, ...
Research Note Incubation of plant material with potassium metabisulfite was found to inhibit fung... more Research Note Incubation of plant material with potassium metabisulfite was found to inhibit fungal infections of explants from grapevines. Grapevine tissue of Vitis labruscana cv. Concord was incubated with sulfite pads containing 0.4 g of potassium metabilsufite for one and two days prior to culturing and evaluated against a control that had been surface sterilized with 0.5 % NaOCI and 70 % ethanol after one week for losses due to microbial contamination. Sulfite fumigation of plant material reduced the incidence of mold infection, particularly in tissue cultures developed from fruit explants which had reductions in contamination as high as 10 fold. Continued attempts to isolate contaminants from cultures intitiated from these explants showed no signs of infection.
Growth of the yeast Brettanomyces/Dekkera in wine can drastically alter the aroma characteristics... more Growth of the yeast Brettanomyces/Dekkera in wine can drastically alter the aroma characteristics to the point where all varietal and regional flavor characteristics are overwhelmed by the flavors produced by these yeasts. To avoid spoilage it is important to know more about the aromas formed by these yeasts and be able to detect the changes in aroma early so that further growth of the yeast and further aroma modification can be avoided. Two groups of commercial wines with suspected "Brett" character were evaluated by two trained panels of judges. The first group included four Cabernet Sauvignon and two Pinot Noir wines; the second included four Cabernet Sauvignon wines. All were evaluated by sensory descriptive analysis and GC/MS 4-ethyl phenol analysis. Characteristic "Brett" aromas such as plastic, burnt plastic, Band-aid (TM), cow manure, barnyard, and horse sweat were summarized by the first group of tasters as 'plastic.' For the second group 'pl...
... Megazyme Total Starch Assay (Amyloglucosidase/a-Amylase Method) Alternate Protocol: Enzymatic... more ... Megazyme Total Starch Assay (Amyloglucosidase/a-Amylase Method) Alternate Protocol: Enzymatic Quantitation of Total Starch (Holm Method) Reagents ... Acree, Eric A. Decker, Michael H. Penner, David S. Reid, Steven J. Schwartz, Charles F. Shoemaker, Denise Smith, Peter ...
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