Mehmet Idil
İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Gynecology and Obstetrics, Department Member
To compare granulosa cell apoptosis in patients with unexplained infertility and tubal factor. Accelerated granulosa cell apoptosis may be the cause of unexplained infertility. Setting was IVF-ET Unit of Cerrahpasa Medical Faculty,... more
To compare granulosa cell apoptosis in patients with unexplained infertility and tubal factor. Accelerated granulosa cell apoptosis may be the cause of unexplained infertility. Setting was IVF-ET Unit of Cerrahpasa Medical Faculty, Istanbul University. GnRH analogs and gonadotropins were used for ovulation induction in patients with unexplained infertility (n=15) and tubal factor (n=15) undergoing in vitro fertilization and embryo transfer (IVF-ET) procedures. Following HCG injection and follicular aspiration, apoptosis of granulosa cells was assessed using the in situ DNA nick end labelling method and apoptosis rate was further determined by flow cytometry. Apoptosis rates were compared between two groups. Mann-Whitney's U-test and Student's t-test were used for statistics. Apoptosis rate was significantly higher in the unexplained infertility group (33.20 +/- 35.62% versus 10.10 +/- 17.23%). Granulosa cell apoptosis seems to have a role in the etiology of unexplained infer...
Research Interests: Nonparametric Statistics, Flow Cytometry, Apoptosis, Probability, Prospective studies, and 16 moreHumans, Female, Ovulation Induction, In Vitro Fertilization, Risk factors, Embryo Transfer, European, Adult, Gonadotropin Releasing Hormone, Study design, Fertilization in Vitro, Risk Factors, Sensitivity and Specificity, Female Infertility, Granulosa Cells, and Follicle stimulating hormone
Kaleli S., Yanıkkaya Demirel G.,Irez T.,Erel C.T.,Senturk L.,Cepni I.,Ocal P.,Oral E.,Idil M.,and Kosebay D. Cell cycle and ploidy changes in granulosa cells obtained from follicular fluid in controlled ovulation induction cycles.19.th Annual meeting of the ESHRE,Madrid,Spain,2003, Human Reproduc...more
ABSTRACT embryo transfer technique, IVF
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We aimed to compare the effects of two different gonadotropins on steroid production in patients with polycystic ovary syndrome (PCOS). The study group comprised 20 infertile patients diagnosed with PCOS who were accepted into in vitro... more
We aimed to compare the effects of two different gonadotropins on steroid production in patients with polycystic ovary syndrome (PCOS). The study group comprised 20 infertile patients diagnosed with PCOS who were accepted into in vitro fertilization-embryo transfer and gamete intra-Fallopian transfer programs. Ten patients were consecutively allocated to a purified urinary follicle stimulating hormone (FSH) administration group while the other ten received human menopausal gonadotropin (hMG). All patients were pretreated with a gonadotropin releasing hormone-agonist. The patients were followed by daily vaginal ultrasonography until at least two follicles reached a diameter of 17 mm or an estradiol value of at least 100 pg/ml per follicle. To induce ovulation, human chorionic gonadotropin was given. On the 3rd day of menstruation, serum estradiol, luteinizing hormone (LH), FSH, total testosterone, androstenedione, dehydroepiandrosterone sulfate (DHEAS), insulin-like growth factor-I and insulin were measured. These same parameters were measured again on the day of follicle aspiration in both serum and follicular fluid. In both groups, the serum levels of estradiol and androstenedione were raised significantly, and on aspiration day the serum level of DHEAS was significantly raised in the FSH group but not in the hMG group. Our findings suggest that in PCOS patients exogenous hMG induces a different steroid synthesis pattern compared to pure FSH, hypothetically by reduction of the delta-5 steroid synthesis pathway in the adrenals and/or in the ovary.