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Development of a Doxycycline Hydrochloride-Loaded Electrospun Nanofibrous Membrane for GTR/GBR Applications

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Jun JiaAuthors Info & Claims

Journal of Nanomaterials, Volume 2016

Page 49

https://doi.org/10.1155/2016/6507459

Published: 01 March 2016 Publication History

Abstract

A drug-loaded membrane was prepared by electrospinning Poly ε-caprolactone PCL with doxycycline hydrochloride DOX 15–25% w/w. Scanning electron microscopy SEM images revealed that fibrous average diameter decreased from 247.16±57.61 nm to 194.43±43.33 nm with the drug proportion increasing from 15% to 20% w/w, while there was no significant difference between 20% and 25% groups. The polymer matrix showed good encapsulation value 58–75% for DOX, and the drug showed an amorphous manner in the polymer matrix. The agar diffusion test revealed that DOX-loaded membranes had an obvious inhibited effect on Aggregatibacter actinomycetemcomitans Aa and Porphyromonas gingivalis Pg, respectively. In vitro release test showed that DOX could persistently be released for a prolonged time more than 28 days, and the DOX level in the eluent steadied at 3–5 μg/mL which was all above the minimum inhibitory concentration MIC of DOX against Aa 0.125 μg/mL and Pg 0.0625 μg/mL. Cytocompatibility, assessed in human periodontal ligament cells hPLCs by MTT-test and the morphology of cells on the surface of DOX-loaded membranes by SEM, indicated that all of the investigated nanofibrous membranes can be used to treat periodontal disease by integrating the GTR/GBR operation and antibiotic therapy. Above all, DOX-loaded nanofibrous membranes could have a persistent inhibited effect on periodontal pathogens to provide a relatively sterile environment for tissue repair and regeneration.

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Cited By

Jia LZhang XXu HHua FHu XXie QWang WJia J(2016)Corrigendum to “Development of a Doxycycline Hydrochloride-Loaded Electrospun Nanofibrous Membrane for GTR/GBR Applications”Journal of Nanomaterials10.1155/2016/67218062016(21)Online publication date: 1-Dec-2016
https://dl.acm.org/doi/10.1155/2016/6721806

Development of a Doxycycline Hydrochloride-Loaded Electrospun Nanofibrous Membrane for GTR/GBR Applications
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  1. Life and medical sciences
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    1. Ad hoc networks

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Published In

cover image Journal of Nanomaterials

Journal of Nanomaterials Volume 2016, Issue

March 2016

807 pages

ISSN:1687-4110

EISSN:1687-4129

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Copyright © Copyright © 2016 Lie-ni Jia et al.

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Hindawi Limited

London, United Kingdom

Publication History

Published: 01 March 2016

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Cited By

Jia LZhang XXu HHua FHu XXie QWang WJia J(2016)Corrigendum to “Development of a Doxycycline Hydrochloride-Loaded Electrospun Nanofibrous Membrane for GTR/GBR Applications”Journal of Nanomaterials10.1155/2016/67218062016(21)Online publication date: 1-Dec-2016
https://dl.acm.org/doi/10.1155/2016/6721806

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