Abstract
It is known that glucose-induced depolarization1â4 of pancreatic B-cells is due to reduced membrane K+-permeability5â8 and is coupled to an increase in the rate of glycolysis9, but there has been no direct evidence linking specific metabolic processes or products to the closing of membrane K+ channels. During patchâclamp studies of proton inhibition of Ca2+-activated K+ channels [GK(Ca)] in B-cells10, we identified a second K+-selective channel which is rapidly and reversibly inhibited by ATP applied to the cytoplasmic surface of the membrane. This channel is spontaneously active in excised patches and frequently coexists with GK(Ca) channels yet is insensitive to membrane potential and to intracellular free Ca2+ and pH. Blocking of the channel is ATP-specific and appears not to require metabolism of the ATP. This ATP-sensitive K+ channel [GK(ATP)] may be a link between metabolism and membrane K+-permeability in pancreatic B-cells.
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Cook, D., Hales, N. Intracellular ATP directly blocks K+ channels in pancreatic B-cells. Nature 311, 271â273 (1984). https://doi.org/10.1038/311271a0
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DOI: https://doi.org/10.1038/311271a0
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