Click through the PLOS taxonomy to find articles in your field.
For more information about PLOS Subject Areas, click here.
Novel Markers to Delineate Murine M1 and M2 Macrophages
Fig 7
Mouse endotoxemia promotes a CD38+ macrophage population.
A. Representative flow plots of CD38 expression in spleen CD11b+F480+ macrophages of mice treated for 12 hours with PBS (top) or LPS (bottom). Spleen macrophages were gradient-enriched (see Materials and Methods) and processed for flow cytometry. Flow plots show the CD11b+F480+ population after gating out dead cells (IndoA+) and neutrophils (Ly6G+). B. Quantification of the results from A (n = 3, representative of three independent experiments). C. Histogram plots comparing CD38 expression by Mean Fluorescence Intensity (MFI) in spleen macrophages (IndoA-Ly6G-CD11b+F480+ gate) from mice treated with PBS (light blue line) or LPS (shaded thick red line). D. Quantification of the results from C (n = 3, representative of three independent experiments). E. Comparison of CD38 expression by MFI within the spleen CD38+ macrophage population (labeled in C) of PBS or LPS-treated mice (n = 3, representative of three independent experiments). ****p<0.0001.