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Mapping of Enzyme Kinetics on a Microfluidic Device
Fig 5
Michaelis-Menten plots of the enzymatic reaction of HRP with AR and H2O2.
The initial rates of the HRP-catalyzed reactions with various concentrations of two substrates are plotted in 3D (A). Based on xz projection (B) and yz projection (C) of the 3D plot, Km and Vmax values are calculated and plotted in (D) and (E). The Km and Vmax values from off-chip reactions are shown in (E).