- Department of Medical Neurobiology, Institute of Medical Research Israel – Canada
The Faculty of Medicine, The Hebrew University of Jerusalem
POB 12272, Ein Kerem Campus
9112102 Jerusalem, Israel - +972-2-675-7385
Joshua A Goldberg
The Hebrew University of Jerusalem, Medical Neurobiology, Faculty Member
- noneedit
- I received my BSc in Physics and PhD in computational and systems neuroscience from the Hebrew University of Jerusale... moreI received my BSc in Physics and PhD in computational and systems neuroscience from the Hebrew University of Jerusalem; and conducted postdoctoral training at the University of Texas at San Antonio. I was a research assistant professor at Northwestern University before establishing an independent laboratory at the Hebrew University in 2013. I have worked on a variety of animal models of Parkinson’s disease and other movement disorders in primates and transgenic mice. My work has focused on the physiological mechanisms underlying neurodegeneration; and the pathophysiological adaptations that occur both in global brain dynamics and in the autonomous activity of neurons affected by these disorders.
I also held a 3-year position in the medical device industry heading the prototyping and ex vivo testing of a vagal nerve stimulation electrode for r treatment of congestive heart failure.edit
Dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) are richly innervated by GABAergic neurons. The postsynaptic effects of GABA on SNc DA neurons are mediated by a mixture of GABAA and GABAB receptors. Although... more
Dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) are richly innervated by GABAergic neurons. The postsynaptic effects of GABA on SNc DA neurons are mediated by a mixture of GABAA and GABAB receptors. Although activation of GABAA receptors inhibits spike generation, the consequences of GABAB receptor activation are less well characterized. To help fill this gap, perforated patch recordings were made from young adult mouse SNc DA neurons. Sustained stimulation of GABAB receptors hyperpolarized SNc DA neurons, as previously described. However, transient stimulation of GABAB receptors by optical uncaging of GABA did not; rather, it reduced the opening of small-conductance, calcium-activated K+ (SK) channels and increased the irregularity of spiking. This modulation was attributable to inhibition of adenylyl cyclase and protein kinase A. Thus, because suppression of SK channel activity increases the probability of burst spiking, transient co-activation of GABAA and G...
Research Interests:
α-Synuclein overexpression (ASOX) drives the formation of toxic aggregates in neurons vulnerable in Parkinson's disease (PD), including dopaminergic neurons of the substantia nigra (SN) and cholinergic neurons of... more
α-Synuclein overexpression (ASOX) drives the formation of toxic aggregates in neurons vulnerable in Parkinson's disease (PD), including dopaminergic neurons of the substantia nigra (SN) and cholinergic neurons of the dorsal motor nucleus of the vagus (DMV). Just as these populations differ in when they exhibit α-synucleinopathies during PD pathogenesis, they could also differ in their physiological responses to ASOX. An ASOX-mediated hyperactivity of SN dopamine neurons, which was caused by oxidative dysfunction of Kv4.3 potassium channels, was recently identified in transgenic (A53T-SNCA) mice overexpressing mutated human α-synuclein. Noting that DMV neurons display extensive α-synucleinopathies earlier than SN dopamine neurons while exhibiting milder cell loss in PD, we aimed to define the electrophysiological properties of DMV neurons in A53T-SNCA mice. We found that DMV neurons maintain normal firing rates in response to ASOX. Moreover, Kv4.3 channels in DMV neurons exhibit no oxidative dysfunction in the A53T-SNCA mice, which could only be recapitulated in wild-type mice by glutathione dialysis. Two-photon imaging of redox-sensitive GFP corroborated the finding that mitochondrial oxidative stress was diminished in DMV neurons in the A53T-SNCA mice. This reduction in oxidative stress resulted from a transcriptional downregulation of voltage-activated (Cav) calcium channels in DMV neurons, which led to a reduction in activity-dependent calcium influx via Cav channels. Thus, ASOX induces a homeostatic remodeling with improved redox signaling in DMV neurons, which could explain the differential vulnerability of SN dopamine and DMV neurons in PD and could promote neuroprotective strategies that emulate endogenous homeostatic responses to ASOX (e.g., stressless pacemaking) in DMV neurons. Overexpression of mutant α-synuclein causes Parkinson's disease, presumably by driving neurodegeneration in vulnerable neuronal target populations. However, the extent of α-synuclein pathology (e.g., Lewy bodies) is not directly related to the degree of neurodegeneration across various vulnerable neuronal populations. Here, we show that, in contrast to dopamine neurons in the substantia nigra, vagal motoneurons do not enhance their excitability and oxidative load in response to chronic mutant α-synuclein overexpression. Rather, by downregulating their voltage-activated calcium channels, vagal motoneurons acquire a stressless form of pacemaking that diminishes mitochondrial and cytosolic oxidative stress. Emulating this endogenous adaptive response to α-synuclein overexpression could lead to novel strategies to protect dopamine neurons and perhaps delay the onset of Parkinson's disease.
Research Interests:
... Dostrovsky, JO. and Murphy, JT., 1988, Single unit analysis of the human ventral thalamic nuclear group: correlation of thalamic " tremor cells" with the 3-6 Hz component of parkinsonian tremor, J Neurosci, 8: 754-764. Levy,... more
... Dostrovsky, JO. and Murphy, JT., 1988, Single unit analysis of the human ventral thalamic nuclear group: correlation of thalamic " tremor cells" with the 3-6 Hz component of parkinsonian tremor, J Neurosci, 8: 754-764. Levy, R ...
Research Interests:
Research Interests: Neuroscience, Physiology, Cognitive Science, Psychophysics, Molecular Biology, and 26 moreBehavior, Systems Neuroscience, Nature, Signal Transduction, Learning, Memory, Dopamine, Computation, Model, Mice, Animals, Calcium channels, Functional Imaging, Cortex, Nature Neuroscience, dopamine receptor D4, Acetylcholine, Synaptic Transmission, Rats, Cell communication, Action Potentials, Neurosciences, Interneurons, Parkinson Disease, Down-Regulation, and Corpus striatum
Salient stimuli redirect attention and suppress ongoing motor activity. This attentional shift is thought to rely upon thalamic signals to the striatum to shift cortically driven action selection, but the network mechanisms underlying... more
Salient stimuli redirect attention and suppress ongoing motor activity. This attentional shift is thought to rely upon thalamic signals to the striatum to shift cortically driven action selection, but the network mechanisms underlying this interaction are unclear. Using a brain slice preparation that preserved cortico- and thalamostriatal connectivity, it was found that activation of thalamostriatal axons in a way that mimicked the response to salient stimuli induced a burst of spikes in striatal cholinergic interneurons that was followed by a pause lasting more than half a second. This patterned interneuron activity triggered a transient, presynaptic suppression of cortical input to both major classes of principal medium spiny neuron (MSN) that gave way to a prolonged enhancement of postsynaptic responsiveness in striatopallidal MSNs controlling motor suppression. This differential regulation of the corticostriatal circuitry provides a neural substrate for attentional shifts and cessation of ongoing motor activity with the appearance of salient environmental stimuli.
Research Interests: Cognitive Science, Patch-clamp and imaging techniques, Thalamus, Cerebral Cortex, Mice, and 17 moreAnimals, Cocaine, Acetylcholine, Synaptic Transmission, Amino Acids, Time Factors, Neuron, Signaling, Neural pathways, Mecamylamine, Action Potentials, Neurosciences, Interneurons, Excitatory Postsynaptic Potentials, Sulpiride, Dopamine antagonists, and Corpus striatum
Research Interests:
Research Interests:
The factors governing neuronal loss in... more
The factors governing neuronal loss in Parkinson's disease (PD) are the subject of continuing speculation and experimental study. In recent years, factors that act on most or all cell types (pan-cellular factors), particularly genetic mutations and environmental toxins, have dominated public discussions of disease aetiology. Although there is compelling evidence supporting an association between disease risk and these factors, the pattern of neuronal pathology and cell loss is difficult to explain without cell-specific factors. This chapter focuses on recent studies showing that the neurons at greatest risk in PD--substantia nigra pars compacta (SNc) dopamine (DA) neurons--have a distinctive physiological phenotype that could contribute to their vulnerability. The opening of L-type calcium channels during autonomous pacemaking results in sustained calcium entry into the cytoplasm of SNc DA neurons, resulting in elevated mitochondrial oxidant stress and susceptibility to toxins used to create animal models of PD. This cell-specific stress could increase the negative consequences of pan-cellular factors that broadly challenge either mitochondrial or proteostatic competence. The availability of well-tolerated, orally deliverable antagonists for L-type calcium channels points to a novel neuroprotective strategy that could complement current attempts to boost mitochondrial function in the early stages of the disease.
Research Interests: Genetics, Cognitive Science, Aging, Calcium, Mitochondria, and 18 moreDopamine, Humans, Mutation, Animals, Cell Death, Animal Model, Substantia nigra, Neurons, Experimental Study, NMDA, Cyclic nucleotides, Bf, Lb, Neurosciences, Parkinson Disease, Substantia nigra pars compacta (SNpc), Neuroprotective Agents, and Calcium Channel Blockers
Research Interests:
Research Interests:
Research Interests:
Research Interests: Neuroscience, Algorithms, Electrophysiology, Animal Behavior, High Frequency, and 16 moreCercopithecus aethiops, Correlation, Female, Animals, Male, Reaction Time, The, Neurons, Arousal, Globus Pallidus, Spikes, Time Factors, Statistical Properties, Macaca fascicularis, Action Potentials, and Poisson Distribution
Research Interests: Neuroscience, Psychology, Electroencephalography, Information Flow, Cerebellum, and 18 moreCerebral Cortex, Animals, Si, Male, LFP, Sleep, Oscillations, Neurons, Transfer Function, Somatosensory Cortex, Rats, Rat, Local Field Potential, Sma, Neural pathways, Membrane Potential, Action Potentials, and Neurosciences
The motor symptoms of Parkinson's disease (PD) are widely thought to arise from an imbalance in the activity of the two major striatal efferent pathways following the loss of... more
The motor symptoms of Parkinson's disease (PD) are widely thought to arise from an imbalance in the activity of the two major striatal efferent pathways following the loss of dopamine (DA) signaling. In striatopallidal, indirect pathway spiny projection neurons (iSPNs), intrinsic excitability rises following the loss of inhibitory D2 receptor signaling. Because these receptors are normally counterbalanced by adenosine A2a adenosine receptors, antagonists of these receptors are being examined as an adjunct to conventional pharmacological therapies. However, little is known about the effects of sustained A2a receptor antagonism on striatal adaptations in PD models. To address this issue, the A2a receptor antagonist SCH58261 was systemically administered to DA-depleted mice. After 5 days of treatment, the effects of SCH58261 on iSPNs were examined in brain slices using electrophysiological and optical approaches. SCH58261 treatment did not prevent spine loss in iSPNs following depletion, but did significantly attenuate alterations in synaptic currents, spine morphology and dendritic excitability. In part, these effects were attributable to the ability of SCH58261 to blunt the effects of DA depletion on cholinergic interneurons, another striatal cell type that co-expresses A2a and D(2) receptors. Collectively, these results suggest that A2a receptor antagonism improves striatal function in PD models by attenuating iSPN adaptations to DA depletion.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
The CardioFit vagal stimulation system has been developed as a proposed therapy for congestive heart failure (CHF). CardioFit is to be implanted in several hundred CHF patients enrolled in the INOVATE-HF clinical trial, an FDA approved... more
The CardioFit vagal stimulation system has been developed as a proposed therapy for congestive heart failure (CHF). CardioFit is to be implanted in several hundred CHF patients enrolled in the INOVATE-HF clinical trial, an FDA approved study. The CardioFit stimulation lead (CSL), which is a cuff electrode that delivers stimulation pulses to the right cervical vagus, was designed to recruit efferent cardiac vagal fibers while minimizing unwanted recruitment of other fibers. This paper presents the CSL and measurements of its recruiting and blocking properties when placed on isolated porcine vagus nerves maintained at an elevated temperature in oxygenated artificial cerebrospinal fluid. Using charge balanced quasi-trapezoidal pulses driven through the CSL, we show in eight out of nine nerves a 63% ± 13% (mean ± SD) unidirectional attenuation of the A-fiber compound action potential attained at a current of 3.0 ± 0.8 mA. The threshold for the activation of A- and B-fibers was found to be 0.3 ± 0.17 mA and 2.5 ± 1.1 mA, respectively. The results presented here should help to guide the optimal parameters used in the upcoming deployment of the CardioFit system.