Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis o... more Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis of atherosclerosis by preventing lipid peroxide generation. The population variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Recent studies have suggested that PON-1 Q192R polymorphism is associated with coronary artery disease (CAD) in different ethnic populations. However to this date, there is no such study in Malaysia. The present study looks at PON-1 Q192R gene polymorphism among CAD patients and healthy controls in Kuantan, Pahang. This study consisted of 187 CAD patients and 188 healthy subjects. PON-1 Q192R genotypes were determined by polymerase chain reaction (PCR) and AlwI restriction enzyme digestion. The Q192R polymorphism was significantly associated with CAD (p = 0.004), where the 192R allele was found to be more frequent in CAD patients than controls (0.618 vs 0.513) with QR genotype being the commonest (45.5%), followed by RR (39.0%) and QQ (15.5%) genotypes. In conclusion, the PON-1 Q192R polymorphism is significantly associated with CAD. This finding indicates that this gene variation is a useful genetic marker for CAD in our population. However, a multicentre study may be required to confirm our findings in Malaysian population.
Introduction: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is ... more Introduction: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is known to inhibit oxidative modification of low density lipoprotein (LDL), thus implicated in the pathogenesis of atherosclerosis and coronary artery disease (CAD). It has been suggested that the variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Nevertheless, even within the same genotype, PON-1 activity and concentration has been shown to vary widely between the different individuals. Therefore, recent studies in various populations have emphasized on the importance of measuring the PON1 activity and concentration in assessing the risk of CAD. The data of such study is however scarce in Malaysia. Objective: The aim of this study was to compare the PON-1 activities and concentration between the healthy controls and CAD patients. Methods: A comparative cross sectional study was carried out on 187 CAD patients in Tengku Ampuan Afzan Hospital, Kuantan and 188 healthy controls. Serum samples were analyzed for PON-1 activities towards paraoxon and phenylacetate as well as for HDLcholesterol. PON1 concentration was expressed as PON1 activity per mmol of HDL. Results: Serum PON-1 activities as well as concentration were found to be lower in CAD patients than in the healthy controls but the results were not significant (p > 0.05). Conclusion: Our finding suggested that PON1 activities and concentration were similar between healthy control and CAD patients in Kuantan, Pahang. A multicentre study may be required to confirm our findings in Malaysian population.
Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis o... more Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis of atherosclerosis by preventing lipid peroxide generation. The population variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Recent studies have suggested that PON-1 Q192R polymorphism is associated with coronary artery disease (CAD) in different ethnic populations. However to this date, there is no such study in Malaysia. The present study looks at PON-1 Q192R gene polymorphism among CAD patients and healthy controls in Kuantan, Pahang. This study consisted of 187 CAD patients and 188 healthy subjects. PON-1 Q192R genotypes were determined by polymerase chain reaction (PCR) and AlwI restriction enzyme digestion. The Q192R polymorphism was significantly associated with CAD (p = 0.004), where the 192R allele was found to be more frequent in CAD patients than controls (0.618 vs 0.513) with QR genotype being the commonest (45.5%), followed by RR (39.0%) and QQ (15.5%) genotypes. In conclusion, the PON-1 Q192R polymorphism is significantly associated with CAD. This finding indicates that this gene variation is a useful genetic marker for CAD in our population. However, a multicentre study may be required to confirm our findings in Malaysian population.
Introduction: Acute myocardial infarction (AMI) is a severe coronary heart disease. Targeted mi... more Introduction: Acute myocardial infarction (AMI) is a severe coronary heart disease. Targeted miRNAs studies implicated two main pathways in the regulation of AMI namely pro-apoptosis (miR-29b and miR-194-5p on PTEN) and pro-necroptosis (miR-325 & miR-105 on RIPK3 ) pathways. This study aims to profile the miRNAs in Healthy Controls, Young AMI, and Mature AMI patients with matching criteria. MATERIALS AND Methods: Total RNA was extracted from plasma and the miRNA expression profiling using small RNA was done on the BGISEQ500 SE5 sequencing platform with BGI sequencing libraries. The sequence data were analysed using Gene Ontology (GO) to determine the function of the differently expressed genes, while Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses were applied to identify the biological pathways in Young AMI against Mature AMI. Results: Of 1497 differentially expressed miRNAs, 1090 miRNAs were upregulated, and 407 miRNAs were downregulated in Young AMI against...
Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis o... more Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis of atherosclerosis by preventing lipid peroxide generation. The population variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Recent studies have suggested that PON-1 Q192R polymorphism is associated with coronary artery disease (CAD) in different ethnic populations. However to this date, there is no such study in Malaysia. The present study looks at PON-1 Q192R gene polymorphism among CAD patients and healthy controls in Kuantan, Pahang. This study consisted of 187 CAD patients and 188 healthy subjects. PON-1 Q192R genotypes were determined by polymerase chain reaction (PCR) and AlwI restriction enzyme digestion. The Q192R polymorphism was significantly associated with CAD (p = 0.004), where the 192R allele was found to be more frequent in CAD patients than controls (0.618 vs 0.513) with QR genotype being the commonest (45.5%), followed by RR (39.0%) and QQ (15.5%) genotypes. In conclusion, the PON-1 Q192R polymorphism is significantly associated with CAD. This finding indicates that this gene variation is a useful genetic marker for CAD in our population. However, a multicentre study may be required to confirm our findings in Malaysian population.
Introduction: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is ... more Introduction: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is known to inhibit oxidative modification of low density lipoprotein (LDL), thus implicated in the pathogenesis of atherosclerosis and coronary artery disease (CAD). It has been suggested that the variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Nevertheless, even within the same genotype, PON-1 activity and concentration has been shown to vary widely between the different individuals. Therefore, recent studies in various populations have emphasized on the importance of measuring the PON1 activity and concentration in assessing the risk of CAD. The data of such study is however scarce in Malaysia. Objective: The aim of this study was to compare the PON-1 activities and concentration between the healthy controls and CAD patients. Methods: A comparative cross sectional study was carried out on 187 CAD patients in Tengku Ampuan Afzan Hospital, Kuantan and 188 healthy controls. Serum samples were analyzed for PON-1 activities towards paraoxon and phenylacetate as well as for HDLcholesterol. PON1 concentration was expressed as PON1 activity per mmol of HDL. Results: Serum PON-1 activities as well as concentration were found to be lower in CAD patients than in the healthy controls but the results were not significant (p > 0.05). Conclusion: Our finding suggested that PON1 activities and concentration were similar between healthy control and CAD patients in Kuantan, Pahang. A multicentre study may be required to confirm our findings in Malaysian population.
Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis o... more Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis of atherosclerosis by preventing lipid peroxide generation. The population variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Recent studies have suggested that PON-1 Q192R polymorphism is associated with coronary artery disease (CAD) in different ethnic populations. However to this date, there is no such study in Malaysia. The present study looks at PON-1 Q192R gene polymorphism among CAD patients and healthy controls in Kuantan, Pahang. This study consisted of 187 CAD patients and 188 healthy subjects. PON-1 Q192R genotypes were determined by polymerase chain reaction (PCR) and AlwI restriction enzyme digestion. The Q192R polymorphism was significantly associated with CAD (p = 0.004), where the 192R allele was found to be more frequent in CAD patients than controls (0.618 vs 0.513) with QR genotype being the commonest (45.5%), followed by RR (39.0%) and QQ (15.5%) genotypes. In conclusion, the PON-1 Q192R polymorphism is significantly associated with CAD. This finding indicates that this gene variation is a useful genetic marker for CAD in our population. However, a multicentre study may be required to confirm our findings in Malaysian population.
Introduction: Acute myocardial infarction (AMI) is a severe coronary heart disease. Targeted mi... more Introduction: Acute myocardial infarction (AMI) is a severe coronary heart disease. Targeted miRNAs studies implicated two main pathways in the regulation of AMI namely pro-apoptosis (miR-29b and miR-194-5p on PTEN) and pro-necroptosis (miR-325 & miR-105 on RIPK3 ) pathways. This study aims to profile the miRNAs in Healthy Controls, Young AMI, and Mature AMI patients with matching criteria. MATERIALS AND Methods: Total RNA was extracted from plasma and the miRNA expression profiling using small RNA was done on the BGISEQ500 SE5 sequencing platform with BGI sequencing libraries. The sequence data were analysed using Gene Ontology (GO) to determine the function of the differently expressed genes, while Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses were applied to identify the biological pathways in Young AMI against Mature AMI. Results: Of 1497 differentially expressed miRNAs, 1090 miRNAs were upregulated, and 407 miRNAs were downregulated in Young AMI against...
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