Introduction: Breast cancer is a leading cause of cancer-related deaths in females[1], and circul... more Introduction: Breast cancer is a leading cause of cancer-related deaths in females[1], and circular RNAs (circRNAs) have emerged as a novel class of noncoding RNAs that play regulatory roles in angiogenesis and cancer progression. The study of the expression patterns and functional roles of circRNAs in breast cancer has become an area of growing interest. The aim of this study was to investigate the relationship between hsa-circ-00001724 gene expression and breast cancer. method: Circular RNAs were extracted from tissue samples and cDNAs were synthesized, followed by RT-PCR of the glyceraldehyde-3-phosphate dehydrogenase gene as an internal control to ensure the quality of the synthesized cDNA samples. Negative controls were used in RT-PCR and qRT-PCR to exclude contamination with genomic DNA and PCR materials, and a positive control test was also performed. The most appropriate primer sequences were selected using OligoAnalyzer software and the NCBI website, and real-time PCR using...
A key aspect of genomic research in the “post‐genome era” is to associate sequence variations wit... more A key aspect of genomic research in the “post‐genome era” is to associate sequence variations with heritable phenotypes. The most common variations in the human genome are single nucleotide polymorphisms (SNPs) that occur approximately once in every 500 to 1,000 bases. Although analyzing the phenotypic outcome of these SNPs is crucial to facilitate large‐scale association studies of genetic diseases, detection
Mitochondrial DNA is suitable for phylogenetic studies. Hence in this study, mitochondrial DNA ge... more Mitochondrial DNA is suitable for phylogenetic studies. Hence in this study, mitochondrial DNA genes in Salmo trutta caspius were sequenced and deposited in Genbank. Six sequences of S.t. caspius mitochondrial ATPase 6 gene with accession nos. LC011387.1, LC154842-LC154847 and the full length of the mitochondrial genome in S.t. caspius (accession no. LC011387.1) were deposited in Genbank. Sequences were aligned between S.t. caspius and Salmo trutta by BLAST program. Although results showed a high homology between both sequences, but single nucleotide variations between ND genes in S.t. caspius and Salmo trutta were observed. The Salmo mitochondrial genome contains one non-coding control region (D-Loop) and two genes for ribosomal RNA, 22 genes encoding tRNAs and thirteen protein-coding genes. S.t. caspius, S. trutta and Salmo salar sequences were converted into a circular map in an online system named Organellar Genome DRAW (http://ogdraw.mpimp-golm.mpg.de). Maps created by OGDRAW were different for S.t. caspius, S. trutta and S. salar. Moreover, Maximum Parsimony method was conducted for evolutionary analysis using MEGA 6.0 program tree between S.t. caspius and other salmonids. Results showed that S.t. caspius and others are covered in a group. Results of evolutionary analysis using Tajima's test were conducted in MEGA 6.0 program for three sequencing including S.t. caspius, S.t. specimen voucher NO. 00 and S.t. fario used as an outgroup for equality of evolutionary rate. Comparison of partial mitochondrial sequence in Iranian S.t. caspius population was performed between three regions [Tonekabon (Cheshmekileh Roud), Ramsar (Safa Roud) and Talesh (Nav roud)]. The results showed that except for one single nucleotide mutation in the partial sequence of mitochondria of S.t. caspius [Ramsar (Safa Roud)], there has been 100% homology between them. In conclusion, the mitochondrial genome homology of S.t. caspius and other salmonids was high, though some SNPs were observed between S.t. caspius and other salmonid species.
In this research, I studied on the single nucleotide polymorphism (SNPs) of Growth hormone gene (... more In this research, I studied on the single nucleotide polymorphism (SNPs) of Growth hormone gene (GH) in Salmo trutta caspius. GH gene in Salmo trutta caspius have been six exons and five introns the full length. Single nucleotide polymorphism (SNPs) of Growth hormone gene (GH) were compared between Salmo trutta caspius and Salmo salar by BLAST-NCBI-Network system. Following to BLAST program, were found three gaps in full length of GH gene, first gap originated to 960 bp (Salmo trutta caspius) and 1610 bp (Salmo salar), second gap originated to 2033 bp (Salmo trutta caspius) and 4206 bp (Salmo salar), third gap 1318 bp (Salmo trutta caspius) and 2637 bp (Salmo salar). The all of gaps were not math with together, but the similarity of nucleotides between gaps were high. In this study we found SNPs in full length of GH gene when compared between Salmo trutta caspius and Salmo salar by BLAST-NCBI-Network system. According my research were found 22 single nucleotide mutations in first gap and 12 single nucleotide mutations in second gap, however there were not any single nucleotide mutation in third gap. For generally similarity of GH gene between Salmo trutta caspius and Salmo salar is high (98%).
Introduction: Breast cancer is a leading cause of cancer-related deaths in females[1], and circul... more Introduction: Breast cancer is a leading cause of cancer-related deaths in females[1], and circular RNAs (circRNAs) have emerged as a novel class of noncoding RNAs that play regulatory roles in angiogenesis and cancer progression. The study of the expression patterns and functional roles of circRNAs in breast cancer has become an area of growing interest. The aim of this study was to investigate the relationship between hsa-circ-00001724 gene expression and breast cancer. method: Circular RNAs were extracted from tissue samples and cDNAs were synthesized, followed by RT-PCR of the glyceraldehyde-3-phosphate dehydrogenase gene as an internal control to ensure the quality of the synthesized cDNA samples. Negative controls were used in RT-PCR and qRT-PCR to exclude contamination with genomic DNA and PCR materials, and a positive control test was also performed. The most appropriate primer sequences were selected using OligoAnalyzer software and the NCBI website, and real-time PCR using...
A key aspect of genomic research in the “post‐genome era” is to associate sequence variations wit... more A key aspect of genomic research in the “post‐genome era” is to associate sequence variations with heritable phenotypes. The most common variations in the human genome are single nucleotide polymorphisms (SNPs) that occur approximately once in every 500 to 1,000 bases. Although analyzing the phenotypic outcome of these SNPs is crucial to facilitate large‐scale association studies of genetic diseases, detection
Mitochondrial DNA is suitable for phylogenetic studies. Hence in this study, mitochondrial DNA ge... more Mitochondrial DNA is suitable for phylogenetic studies. Hence in this study, mitochondrial DNA genes in Salmo trutta caspius were sequenced and deposited in Genbank. Six sequences of S.t. caspius mitochondrial ATPase 6 gene with accession nos. LC011387.1, LC154842-LC154847 and the full length of the mitochondrial genome in S.t. caspius (accession no. LC011387.1) were deposited in Genbank. Sequences were aligned between S.t. caspius and Salmo trutta by BLAST program. Although results showed a high homology between both sequences, but single nucleotide variations between ND genes in S.t. caspius and Salmo trutta were observed. The Salmo mitochondrial genome contains one non-coding control region (D-Loop) and two genes for ribosomal RNA, 22 genes encoding tRNAs and thirteen protein-coding genes. S.t. caspius, S. trutta and Salmo salar sequences were converted into a circular map in an online system named Organellar Genome DRAW (http://ogdraw.mpimp-golm.mpg.de). Maps created by OGDRAW were different for S.t. caspius, S. trutta and S. salar. Moreover, Maximum Parsimony method was conducted for evolutionary analysis using MEGA 6.0 program tree between S.t. caspius and other salmonids. Results showed that S.t. caspius and others are covered in a group. Results of evolutionary analysis using Tajima's test were conducted in MEGA 6.0 program for three sequencing including S.t. caspius, S.t. specimen voucher NO. 00 and S.t. fario used as an outgroup for equality of evolutionary rate. Comparison of partial mitochondrial sequence in Iranian S.t. caspius population was performed between three regions [Tonekabon (Cheshmekileh Roud), Ramsar (Safa Roud) and Talesh (Nav roud)]. The results showed that except for one single nucleotide mutation in the partial sequence of mitochondria of S.t. caspius [Ramsar (Safa Roud)], there has been 100% homology between them. In conclusion, the mitochondrial genome homology of S.t. caspius and other salmonids was high, though some SNPs were observed between S.t. caspius and other salmonid species.
In this research, I studied on the single nucleotide polymorphism (SNPs) of Growth hormone gene (... more In this research, I studied on the single nucleotide polymorphism (SNPs) of Growth hormone gene (GH) in Salmo trutta caspius. GH gene in Salmo trutta caspius have been six exons and five introns the full length. Single nucleotide polymorphism (SNPs) of Growth hormone gene (GH) were compared between Salmo trutta caspius and Salmo salar by BLAST-NCBI-Network system. Following to BLAST program, were found three gaps in full length of GH gene, first gap originated to 960 bp (Salmo trutta caspius) and 1610 bp (Salmo salar), second gap originated to 2033 bp (Salmo trutta caspius) and 4206 bp (Salmo salar), third gap 1318 bp (Salmo trutta caspius) and 2637 bp (Salmo salar). The all of gaps were not math with together, but the similarity of nucleotides between gaps were high. In this study we found SNPs in full length of GH gene when compared between Salmo trutta caspius and Salmo salar by BLAST-NCBI-Network system. According my research were found 22 single nucleotide mutations in first gap and 12 single nucleotide mutations in second gap, however there were not any single nucleotide mutation in third gap. For generally similarity of GH gene between Salmo trutta caspius and Salmo salar is high (98%).
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