Kochegarov A., Moses A., Lemanski L.F. Identification of Human Myofibril-inducing RNA. Mol. Biol.... more Kochegarov A., Moses A., Lemanski L.F. Identification of Human Myofibril-inducing RNA. Mol. Biol. Cell 23, (suppl), Denver, CO, 2011, Abstract 719 Department of Biological and Environmental Sciences, Texas A&M University, Commerce, TX 75429-3011 The axolotl, Ambystoma mexicanum, carries a c recessive lethal mutation that prevents normal embryonic heart development. In the heart of mutant embryos, expression of tropomyosin is reduced, the development of cardiac sarcomeres fails and the heart does not contract. Myofibril-Inducing RNA (MIR) from anterior endoderm of normal axolotl embryos restores expression of tropomysin, promotes the formation of sarcomeric myofibrils and induces heart contractions. RNA from fetal and adult human hearts also rescues the axolotl embryonic mutant hearts. To identify the active RNA(s) in humans, we cloned the human heart RNAs and created a RNA library. Two human RNA clones were found to promote mutant axolotl heart development. The hearts treated with t...
Background: A recessive mutation "c" in the Mexican axolotl, Ambystoma mexicanum, results in the ... more Background: A recessive mutation "c" in the Mexican axolotl, Ambystoma mexicanum, results in the failure of normal heart development. In homozygous recessive embryos, the hearts do not have organized myofibrils and fail to beat. In our previous studies, we identified a noncoding Myofibril-Inducing RNA (MIR) from axolotls which promotes myofibril formation and rescues heart development. Results: We randomly cloned RNAs from fetal human heart. RNA from clone #291 promoted myofibril formation and induced heart development of mutant axolotls in organ culture. This RNA induced expression of cardiac markers in mutant hearts: tropomyosin, troponin and α-syntrophin. This cloned RNA matches in partial sequence alignment to human microRNA-499a and b, although it differs in length. We have concluded that this cloned RNA is unique in its length, but is still related to the microRNA-499 family. We have named this unique RNA, microRNA-499c. Thus, we will refer to this RNA derived from clone #291 as microRNA-499c throughout the rest of the paper. Conclusions: This new form, microRNA-499c, plays an important role in cardiac development.
Kochegarov A., Moses A., Lemanski L.F. Identification of Human Myofibril-inducing RNA. Mol. Biol.... more Kochegarov A., Moses A., Lemanski L.F. Identification of Human Myofibril-inducing RNA. Mol. Biol. Cell 23, (suppl), Denver, CO, 2011, Abstract 719 Department of Biological and Environmental Sciences, Texas A&M University, Commerce, TX 75429-3011 The axolotl, Ambystoma mexicanum, carries a c recessive lethal mutation that prevents normal embryonic heart development. In the heart of mutant embryos, expression of tropomyosin is reduced, the development of cardiac sarcomeres fails and the heart does not contract. Myofibril-Inducing RNA (MIR) from anterior endoderm of normal axolotl embryos restores expression of tropomysin, promotes the formation of sarcomeric myofibrils and induces heart contractions. RNA from fetal and adult human hearts also rescues the axolotl embryonic mutant hearts. To identify the active RNA(s) in humans, we cloned the human heart RNAs and created a RNA library. Two human RNA clones were found to promote mutant axolotl heart development. The hearts treated with t...
Background: A recessive mutation "c" in the Mexican axolotl, Ambystoma mexicanum, results in the ... more Background: A recessive mutation "c" in the Mexican axolotl, Ambystoma mexicanum, results in the failure of normal heart development. In homozygous recessive embryos, the hearts do not have organized myofibrils and fail to beat. In our previous studies, we identified a noncoding Myofibril-Inducing RNA (MIR) from axolotls which promotes myofibril formation and rescues heart development. Results: We randomly cloned RNAs from fetal human heart. RNA from clone #291 promoted myofibril formation and induced heart development of mutant axolotls in organ culture. This RNA induced expression of cardiac markers in mutant hearts: tropomyosin, troponin and α-syntrophin. This cloned RNA matches in partial sequence alignment to human microRNA-499a and b, although it differs in length. We have concluded that this cloned RNA is unique in its length, but is still related to the microRNA-499 family. We have named this unique RNA, microRNA-499c. Thus, we will refer to this RNA derived from clone #291 as microRNA-499c throughout the rest of the paper. Conclusions: This new form, microRNA-499c, plays an important role in cardiac development.
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