Preparative Biochemistry & Biotechnology, Mar 24, 2009
There are relatively few studies on the properties of proteins that comprise a major part of the ... more There are relatively few studies on the properties of proteins that comprise a major part of the safflower seed. The biochemical and functional properties of these proteins have not been fully discovered. In this study, safflower seed proteins were obtained by isoelectric precipitation in two fractions. One of the fractions (Fraction-1) was obtained at pH 10 and the other fraction was obtained, as the protein supernatant separate from the pH precipitate, by ultra filtration (Fraction-2). Functional and biochemical properties of both of fractions were investigated. Gel permeation chromatography (GPC) was applied to both fractions. GPC shows that high-molecular weight constituents are present only in fraction 1, whereas fraction 2 consists of proteins with lower molecular weights in comparison with protein standards. Sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis in the presence of mercaptoethanol and SDS with protein weight markers was applied to both of the fractions. The proteins in both of the fractions were separated and stained with Commassie Brillant blue R-250 dye on the PAGE gel. The molecular weight (M(w)) of each protein band was determined graphically by plotting Log M(w) and relative mobilities (R(f)) using GS-300 scanning densitometry and a suitable computer program.
Bu araştırma, 2018 ve 2019 yılında Kayseri ekolojik koşullarında, farklı su oranları (S) vebetain... more Bu araştırma, 2018 ve 2019 yılında Kayseri ekolojik koşullarında, farklı su oranları (S) vebetain (B) uygulamalarının şeker pancarında (Beta vulgaris L.) gelişme dönemlerine veagronomik özelliklere etkisini belirlemek amacıyla yürütülmüştür. Araştırma, 3 tekerrürlüolarak kurulmuştur. Denemede şeker pancarında 3 farklı su oranı (S; %30, %70 ve %100), 4farklı betain dozu (B; 0, 125, 250, 375 ml/da) ve bu dozların kombinasyonları uygulanmıştır.Çalışmada elde edilen sonuçlara göre; en yüksek pancar verimi 2018 yılında S100×B375 ml/dainteraksiyonunda (15.92 kg/da) ve en yüksek şeker varlığı ise 2018 yılında S30×B375interaksiyonunda (%20.84) elde edilmiştir. En düşük α-amino-N oranı 2019 yılında S100×B250interaksiyonunda (%1.71), sodyum (Na) oranı 2019 yılında S100×B250 interaksiyonunda(%0.88) ve en düşük potasyum (K) oranı 2019 yılında S100×B250 (%3.47) saptanmıştır. Farklısu ve betain oranlarının şeker pancarında verim ve kalite özelliklerine etkisi önemlibulunmuştur. Su oranının artmas...
OZET Tarim sektoru ve insan beslenmesi acisindan bitkisel yag uretimi vazgecilmez bir oneme sahip... more OZET Tarim sektoru ve insan beslenmesi acisindan bitkisel yag uretimi vazgecilmez bir oneme sahiptir. Turkiye'de toplam yag uretiminin %87'si bitkisel kaynakli yaglardan olusmaktadir. Bitkisel yag uretimi, 12 farkli bitkinin tarimiyla elde edilmektedir. 2001 yili degerlerine gore, Turkiye'de uretilen yagli tohumlarin %62,3'u cigitten, %30'u ise ayciceginden elde edilmistir. Ulkemizde ham yag uretimi 2000 yilinda 836.000 ton olarak gerceklesmistir. Buna karsin Turkiye ham yag bakimindan her yil 600-700 bin ton, yagli tohum olarak da 1,1-1,2 milyon ton acik vermektedir. Bu nedenle ulkemiz bitkisel yag acisindan ithalata bagimli durumdadir. Turkiye'nin bitkisel yag uretimi cercevesinde Trakya, hem yagli tohum uretimi hem de bitkisel yag sanayisi bakimindan cok onemli bir bolgedir. Yagli tohumlarin onemli bitkisi olan ayciceginin Turkiye uretiminin yaklasik %60'i bu bolgede gerceklesmektedir. Trakya Bolgesi'nde 3000 kisiyi istihdam eden 61 adet bitkisel y...
World Journal of Biology Pharmacy and Health Sciences, 2021
The use of certain types of microorganisms or their metabolic products is the basis for the devel... more The use of certain types of microorganisms or their metabolic products is the basis for the development of new technologies, which on the one hand can contribute to the standardization of the production process and achieve uniform quality, and on the other hand to obtain quality and safe products with extended shelf life. On the world market for food and beverages, today can be find a wide range of commercial, fermented products in which production and sustainability certain microorganisms are participate. The interest in the new methods of biological protection of food, as well as the scientific principles for achieving health of the products has increased significantly in recent years. The increase in the need for quality and health-safe food has led to an increased interest in the use of starter cultures in the food industry, and especially in the meat industry. With the use of the starter cultures, good quality of the products is achieved. At the same time a safe product is obta...
Preparative Biochemistry & Biotechnology, Mar 24, 2009
There are relatively few studies on the properties of proteins that comprise a major part of the ... more There are relatively few studies on the properties of proteins that comprise a major part of the safflower seed. The biochemical and functional properties of these proteins have not been fully discovered. In this study, safflower seed proteins were obtained by isoelectric precipitation in two fractions. One of the fractions (Fraction-1) was obtained at pH 10 and the other fraction was obtained, as the protein supernatant separate from the pH precipitate, by ultra filtration (Fraction-2). Functional and biochemical properties of both of fractions were investigated. Gel permeation chromatography (GPC) was applied to both fractions. GPC shows that high-molecular weight constituents are present only in fraction 1, whereas fraction 2 consists of proteins with lower molecular weights in comparison with protein standards. Sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) electrophoresis in the presence of mercaptoethanol and SDS with protein weight markers was applied to both of the fractions. The proteins in both of the fractions were separated and stained with Commassie Brillant blue R-250 dye on the PAGE gel. The molecular weight (M(w)) of each protein band was determined graphically by plotting Log M(w) and relative mobilities (R(f)) using GS-300 scanning densitometry and a suitable computer program.
Bu araştırma, 2018 ve 2019 yılında Kayseri ekolojik koşullarında, farklı su oranları (S) vebetain... more Bu araştırma, 2018 ve 2019 yılında Kayseri ekolojik koşullarında, farklı su oranları (S) vebetain (B) uygulamalarının şeker pancarında (Beta vulgaris L.) gelişme dönemlerine veagronomik özelliklere etkisini belirlemek amacıyla yürütülmüştür. Araştırma, 3 tekerrürlüolarak kurulmuştur. Denemede şeker pancarında 3 farklı su oranı (S; %30, %70 ve %100), 4farklı betain dozu (B; 0, 125, 250, 375 ml/da) ve bu dozların kombinasyonları uygulanmıştır.Çalışmada elde edilen sonuçlara göre; en yüksek pancar verimi 2018 yılında S100×B375 ml/dainteraksiyonunda (15.92 kg/da) ve en yüksek şeker varlığı ise 2018 yılında S30×B375interaksiyonunda (%20.84) elde edilmiştir. En düşük α-amino-N oranı 2019 yılında S100×B250interaksiyonunda (%1.71), sodyum (Na) oranı 2019 yılında S100×B250 interaksiyonunda(%0.88) ve en düşük potasyum (K) oranı 2019 yılında S100×B250 (%3.47) saptanmıştır. Farklısu ve betain oranlarının şeker pancarında verim ve kalite özelliklerine etkisi önemlibulunmuştur. Su oranının artmas...
OZET Tarim sektoru ve insan beslenmesi acisindan bitkisel yag uretimi vazgecilmez bir oneme sahip... more OZET Tarim sektoru ve insan beslenmesi acisindan bitkisel yag uretimi vazgecilmez bir oneme sahiptir. Turkiye'de toplam yag uretiminin %87'si bitkisel kaynakli yaglardan olusmaktadir. Bitkisel yag uretimi, 12 farkli bitkinin tarimiyla elde edilmektedir. 2001 yili degerlerine gore, Turkiye'de uretilen yagli tohumlarin %62,3'u cigitten, %30'u ise ayciceginden elde edilmistir. Ulkemizde ham yag uretimi 2000 yilinda 836.000 ton olarak gerceklesmistir. Buna karsin Turkiye ham yag bakimindan her yil 600-700 bin ton, yagli tohum olarak da 1,1-1,2 milyon ton acik vermektedir. Bu nedenle ulkemiz bitkisel yag acisindan ithalata bagimli durumdadir. Turkiye'nin bitkisel yag uretimi cercevesinde Trakya, hem yagli tohum uretimi hem de bitkisel yag sanayisi bakimindan cok onemli bir bolgedir. Yagli tohumlarin onemli bitkisi olan ayciceginin Turkiye uretiminin yaklasik %60'i bu bolgede gerceklesmektedir. Trakya Bolgesi'nde 3000 kisiyi istihdam eden 61 adet bitkisel y...
World Journal of Biology Pharmacy and Health Sciences, 2021
The use of certain types of microorganisms or their metabolic products is the basis for the devel... more The use of certain types of microorganisms or their metabolic products is the basis for the development of new technologies, which on the one hand can contribute to the standardization of the production process and achieve uniform quality, and on the other hand to obtain quality and safe products with extended shelf life. On the world market for food and beverages, today can be find a wide range of commercial, fermented products in which production and sustainability certain microorganisms are participate. The interest in the new methods of biological protection of food, as well as the scientific principles for achieving health of the products has increased significantly in recent years. The increase in the need for quality and health-safe food has led to an increased interest in the use of starter cultures in the food industry, and especially in the meat industry. With the use of the starter cultures, good quality of the products is achieved. At the same time a safe product is obta...
Uploads
Papers by Aziz ŞATANA