The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, 2007
This preliminary work was designed to study, using routine procedures for light and transmission ... more This preliminary work was designed to study, using routine procedures for light and transmission electron microscopy, the presence of rodlet cells (RCs) in or near the sensory systems of 12 adult specimens (4.0 +/- 1.2 cm, LT +/- SD) of zebrafish, Danio rerio Hamilton, 1822. Rodlet cells, characterized by a distinctive cell cortex (range, 0.4-1.5 microm in thickness) and conspicuous inclusions named "rodlets," have a round to ovoid nucleus with irregular outline. Mature RCs are 11.5 +/- 1.2 microm (mean +/- SD) long and 7.8 +/- 1.1 microm (mean +/- SD) wide. These cells are more numerous near neuromasts enclosed by an epithelial roof and/or ossified canal wall. In contrast, very few RCs were noticed near superficial neuromasts. Based on the presence of RCs around the two cranial neuromasts of each fish, a variable number from 1 to 15 rodlet cells was found (10.4 +/- 3.6, mean +/- SD). The RCs were located 1.5 microm (minimal) to 73.3 microm (maximal) from the neuromast (27.9 +/- 17.2, mean +/- SD). Moreover, RCs were found in olfactory epithelium and in proximity to some taste buds. Interestingly, RCs were absent in the inner ear, eye, and brain.
Morphological identification of tapeworm species at larval stages (procercoids and cysticercoids)... more Morphological identification of tapeworm species at larval stages (procercoids and cysticercoids) is often difficult because few diagnostic characters are available. In the present study, a molecular approach (sequencing of partial 18S rDNA gene) was used to evaluate the genetic similarity between adult specimens of Cyathocephalus truncatus (Pallas, 1871) (Cestoda: Spathebothriidea) found in fish, its definitive host, and procercoids of the same species recovered from amphipod, Echinogammarus stammeri (Karaman, 1931). Furthermore, cestode cysticercoids of uncertain species were found in the amphipod's hemocoel. The sequences obtained from adults and procercoids were identical, and even very similar to those of C. truncatus available in GenBank, whereas the sequences obtained from cysticercoids differed significantly from those of adults and procercoids, indicating that these larvae belong to another species; later it was demonstrated that they were cysticercoids of Microsomacanthus pachycephala (Linstow, 1972), a cestode of the Hymenolepididae (Cyclophyllidea). The results of this investigation show that the comparison of nucleotide sequence data may avoid misclassification of developmental stages of parasites, which use the same intermediate host.
The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, 2007
This preliminary work was designed to study, using routine procedures for light and transmission ... more This preliminary work was designed to study, using routine procedures for light and transmission electron microscopy, the presence of rodlet cells (RCs) in or near the sensory systems of 12 adult specimens (4.0 +/- 1.2 cm, LT +/- SD) of zebrafish, Danio rerio Hamilton, 1822. Rodlet cells, characterized by a distinctive cell cortex (range, 0.4-1.5 microm in thickness) and conspicuous inclusions named "rodlets," have a round to ovoid nucleus with irregular outline. Mature RCs are 11.5 +/- 1.2 microm (mean +/- SD) long and 7.8 +/- 1.1 microm (mean +/- SD) wide. These cells are more numerous near neuromasts enclosed by an epithelial roof and/or ossified canal wall. In contrast, very few RCs were noticed near superficial neuromasts. Based on the presence of RCs around the two cranial neuromasts of each fish, a variable number from 1 to 15 rodlet cells was found (10.4 +/- 3.6, mean +/- SD). The RCs were located 1.5 microm (minimal) to 73.3 microm (maximal) from the neuromast (27.9 +/- 17.2, mean +/- SD). Moreover, RCs were found in olfactory epithelium and in proximity to some taste buds. Interestingly, RCs were absent in the inner ear, eye, and brain.
Morphological identification of tapeworm species at larval stages (procercoids and cysticercoids)... more Morphological identification of tapeworm species at larval stages (procercoids and cysticercoids) is often difficult because few diagnostic characters are available. In the present study, a molecular approach (sequencing of partial 18S rDNA gene) was used to evaluate the genetic similarity between adult specimens of Cyathocephalus truncatus (Pallas, 1871) (Cestoda: Spathebothriidea) found in fish, its definitive host, and procercoids of the same species recovered from amphipod, Echinogammarus stammeri (Karaman, 1931). Furthermore, cestode cysticercoids of uncertain species were found in the amphipod's hemocoel. The sequences obtained from adults and procercoids were identical, and even very similar to those of C. truncatus available in GenBank, whereas the sequences obtained from cysticercoids differed significantly from those of adults and procercoids, indicating that these larvae belong to another species; later it was demonstrated that they were cysticercoids of Microsomacanthus pachycephala (Linstow, 1972), a cestode of the Hymenolepididae (Cyclophyllidea). The results of this investigation show that the comparison of nucleotide sequence data may avoid misclassification of developmental stages of parasites, which use the same intermediate host.
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Papers by Silvia Capuano