Journal of ethnobiology and ethnomedicine, Jan 7, 2006
An ethnobotanical survey was undertaken to collect information from traditional healers on the us... more An ethnobotanical survey was undertaken to collect information from traditional healers on the use of medicinal plants in Kancheepuram district of Tamil Nadu during October 2003 to April 2004. The indigenous knowledge of local traditional healers and the native plants used for medicinal purposes were collected through questionnaire and personal interviews during field trips.The investigation revealed that, the traditional healers used 85 species of plants distributed in 76 genera belonging to 41 families to treat various diseases. The documented medicinal plants were mostly used to cure skin diseases, poison bites, stomachache and nervous disorders. In this study the most dominant family was Euphorbiaceae and leaves were most frequently used for the treatment of diseases.This study showed that many people in the studied parts of Kancheepuram district still continue to depend on medicinal plants at least for the treatment of primary healthcare. The traditional healers are dwindling i...
An ethnobotanical survey was undertaken to collect information from traditional healers on the us... more An ethnobotanical survey was undertaken to collect information from traditional healers on the use of medicinal plants in Kancheepuram district of Tamil Nadu during October 2003 to April 2004. The indigenous knowledge of local traditional healers and the native plants used for medicinal purposes were collected through questionnaire and personal interviews during field trips. The investigation revealed that, the traditional healers used 85 species of plants distributed in 76 genera belonging to 41 families to treat various diseases. The documented medicinal plants were mostly used to cure skin diseases, poison bites, stomachache and nervous disorders. In this study the most dominant family was Euphorbiaceae and leaves were most frequently used for the treatment of diseases. This study showed that many people in the studied parts of Kancheepuram district still continue to depend on medicinal plants at least for the treatment of primary healthcare. The traditional healers are dwindling...
The antibacterial activity of an extract from Pergularia daemia leaves was tested against pathoge... more The antibacterial activity of an extract from Pergularia daemia leaves was tested against pathogenic bacteria. The test organisms were Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Proteus vulgaris. The antibacterial activity of the crude extract was determined using the disc diffusion method and the MIC of the isolated compound was tested by the broth microdilution method. The crude extract showed activity against Bacillus subtilis (15 mm) Staphylococcus aureus (17 mm) and Proteus vulgaris (20 mm). Minimum inhibitory concentrations for the ethyl acetate crude extract were determined against Staphylococcus aureus (300 µg/ml), Bacillus subtilis (200 µg/ml) and Proteus vulgaris (500 µg/ml). The active extract was separated by column chromatography and six fractions were collected. Fraction V yielded a new compound, 6-(4, 7 dihydroxy-heptyl) quinone, which showed antibacterial activity. The minimum inhibitory concentrations were recorded against Staphylococcus aureus (...
Hexane and methanol extract of Acorus calamus root and ethyl acetate extract of Wedelia calendula... more Hexane and methanol extract of Acorus calamus root and ethyl acetate extract of Wedelia calendulacea leaves showed significant ovicidal activity while ethyl acetate extract of Artemisia nilagrica and hexane extract of A. calamus showed significant ovipositional deterrent activity against Spodoptera litura. Hexane, ethyl acetate and methanol extract of A. nilagrica and hexane extract of A. calamus root showed significant insecticidal activity against S. litura.
A plant oil formulation (POF) consisting of calamus, cinnamon, citronella, clove, eucalyptus, lem... more A plant oil formulation (POF) consisting of calamus, cinnamon, citronella, clove, eucalyptus, lemon, menthe, orange oils and natural Camphor 10% was evaluated for its repellent potential against Aedes aegypti (L.), Anopheles stephensi Liston and Culex quinquefasciatus Say at 250 and 15.25 ppm. At 250 ppm concentrations the POF provided 100% protection from bites of Anopheles stephensi up to a mean time of 6 hours and 30 minutes while for C. quinquefasciatus and A. aegypti, it was 5 h 30 min. At 15.62 ppm it also exhibited more than 74% mean protection against C. quinquefasciatus and A. aegypti while it was 62.88% effective against A. stephensi. Ethanol which was used for suspension was kept as negative control and DEET (25 mg) served as positive control. The study concludes that this formulation could be used to repel these insects.
Bioefficacy of leaf and root extracts of Aristolochia tagala Cham. at different concentrations wa... more Bioefficacy of leaf and root extracts of Aristolochia tagala Cham. at different concentrations was evaluated at room temperature against Spodoptera litura Fab. Effects on feeding, larvicidal and pupicidal activities and larval-pupal duration were studied. Higher antifeedant activity (56.06%), lethal concentration for feeding inhibition (3.69%), larvicidal (40.66%), pupicidal (28%), total mortality (68.66%) and prolonged larval-pupal duration (12.04-13.08 days) were observed in ethyl acetate leaf extract at 5.0% concentration. Dose dependant effect of test extracts was observed. This plant could be used to isolate active principles and to develop a new botanical formulation in pest management programmes.
Asian Pacific Journal of Tropical Biomedicine, 2012
To evaluate the efficacy of Atalantia monophylla (A. monophylla) leaf in different solvent crude ... more To evaluate the efficacy of Atalantia monophylla (A. monophylla) leaf in different solvent crude extracts and fractions against eggs of Spodoptera litura (S. litura). Hexane, ethyl acetate and chloroform solvent extracts of A. monophylla leaf and 12 fractions from hexane extract were screened at 5.0%, 2.5%, 1.0% and 0.5% for crude extracts and 1 000, 500, 250 and 125 mg/kg for fractions against the eggs of S. litura for the ovicidal activity. LC50 and LC90 were calculated using probit analysis. Hexane crude extract showed maximum ovicidal activity of 61.94% at 5.0% concentration with a correlation value of r (2)=0.81, and least LC50 value of 3.06%. Hexane extract was fractionated using silica gel column chromatography and 12 fractions were obtained. Fraction 9 was active which showed maximum ovicidal activity of 75.61% at 1 000 mg/kg with the LC50 value of 318.65 mg/kg and LC90 value of 1 473.31 mg/kg. In linear regression analysis, significant and high correlation (r (2)=0.81%) was seen between concentration and ovicidal activity of hexane crude extracts and its active fraction. As per our knowledge, this is the first report for ovicidal activity of A. monophylla against S. litura, A. monophylla could be used for the management of S. litura and other insect pests.
ABSTRACT Fractions derived from Clerodendrum phlomidis Linn. F. and isolated compounds from chlor... more ABSTRACT Fractions derived from Clerodendrum phlomidis Linn. F. and isolated compounds from chloroform extract were evaluated against Earias vittella Fab. for their ovicidal and oviposition deterrent activities. There were 12 fractions obtained with silica gel column chromatographic techniques using different solvent systems. Among the fractions, fraction 5 recorded maximum ovicidal activity of 68.49% and oviposition deterrent activity of 100% at 1000 ppm concentration followed by 500 ppm concentration. The active fraction was further purified using HPLC. The purified fraction was subjected to UV, 1HNMR, 13CNMR, IR and EI-Mass spectroscopy and their spectral data confirmed the compound pectolinaringenin which belongs to the flavonoids. The compound pectolinaringenin was screened for its ovicidal and oviposition deterrent activities. It showed maximum ovicidal activity of 64.29% and oviposition deterrent activity of 100% at 100 ppm concentration against E. vittella. To the best of our knowledge, this is the first report of fractions and isolated compounds from C. phlomidis evaluated against E. vittella for their ovicidal and oviposition deterrent activities. The results from the present investigation clearly suggest that the active fraction or the isolated compound could be used to develop a novel pesticidal formulation to control economically important agricultural pests.
Journal of ethnobiology and ethnomedicine, Jan 7, 2006
An ethnobotanical survey was undertaken to collect information from traditional healers on the us... more An ethnobotanical survey was undertaken to collect information from traditional healers on the use of medicinal plants in Kancheepuram district of Tamil Nadu during October 2003 to April 2004. The indigenous knowledge of local traditional healers and the native plants used for medicinal purposes were collected through questionnaire and personal interviews during field trips.The investigation revealed that, the traditional healers used 85 species of plants distributed in 76 genera belonging to 41 families to treat various diseases. The documented medicinal plants were mostly used to cure skin diseases, poison bites, stomachache and nervous disorders. In this study the most dominant family was Euphorbiaceae and leaves were most frequently used for the treatment of diseases.This study showed that many people in the studied parts of Kancheepuram district still continue to depend on medicinal plants at least for the treatment of primary healthcare. The traditional healers are dwindling i...
An ethnobotanical survey was undertaken to collect information from traditional healers on the us... more An ethnobotanical survey was undertaken to collect information from traditional healers on the use of medicinal plants in Kancheepuram district of Tamil Nadu during October 2003 to April 2004. The indigenous knowledge of local traditional healers and the native plants used for medicinal purposes were collected through questionnaire and personal interviews during field trips. The investigation revealed that, the traditional healers used 85 species of plants distributed in 76 genera belonging to 41 families to treat various diseases. The documented medicinal plants were mostly used to cure skin diseases, poison bites, stomachache and nervous disorders. In this study the most dominant family was Euphorbiaceae and leaves were most frequently used for the treatment of diseases. This study showed that many people in the studied parts of Kancheepuram district still continue to depend on medicinal plants at least for the treatment of primary healthcare. The traditional healers are dwindling...
The antibacterial activity of an extract from Pergularia daemia leaves was tested against pathoge... more The antibacterial activity of an extract from Pergularia daemia leaves was tested against pathogenic bacteria. The test organisms were Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Proteus vulgaris. The antibacterial activity of the crude extract was determined using the disc diffusion method and the MIC of the isolated compound was tested by the broth microdilution method. The crude extract showed activity against Bacillus subtilis (15 mm) Staphylococcus aureus (17 mm) and Proteus vulgaris (20 mm). Minimum inhibitory concentrations for the ethyl acetate crude extract were determined against Staphylococcus aureus (300 µg/ml), Bacillus subtilis (200 µg/ml) and Proteus vulgaris (500 µg/ml). The active extract was separated by column chromatography and six fractions were collected. Fraction V yielded a new compound, 6-(4, 7 dihydroxy-heptyl) quinone, which showed antibacterial activity. The minimum inhibitory concentrations were recorded against Staphylococcus aureus (...
Hexane and methanol extract of Acorus calamus root and ethyl acetate extract of Wedelia calendula... more Hexane and methanol extract of Acorus calamus root and ethyl acetate extract of Wedelia calendulacea leaves showed significant ovicidal activity while ethyl acetate extract of Artemisia nilagrica and hexane extract of A. calamus showed significant ovipositional deterrent activity against Spodoptera litura. Hexane, ethyl acetate and methanol extract of A. nilagrica and hexane extract of A. calamus root showed significant insecticidal activity against S. litura.
A plant oil formulation (POF) consisting of calamus, cinnamon, citronella, clove, eucalyptus, lem... more A plant oil formulation (POF) consisting of calamus, cinnamon, citronella, clove, eucalyptus, lemon, menthe, orange oils and natural Camphor 10% was evaluated for its repellent potential against Aedes aegypti (L.), Anopheles stephensi Liston and Culex quinquefasciatus Say at 250 and 15.25 ppm. At 250 ppm concentrations the POF provided 100% protection from bites of Anopheles stephensi up to a mean time of 6 hours and 30 minutes while for C. quinquefasciatus and A. aegypti, it was 5 h 30 min. At 15.62 ppm it also exhibited more than 74% mean protection against C. quinquefasciatus and A. aegypti while it was 62.88% effective against A. stephensi. Ethanol which was used for suspension was kept as negative control and DEET (25 mg) served as positive control. The study concludes that this formulation could be used to repel these insects.
Bioefficacy of leaf and root extracts of Aristolochia tagala Cham. at different concentrations wa... more Bioefficacy of leaf and root extracts of Aristolochia tagala Cham. at different concentrations was evaluated at room temperature against Spodoptera litura Fab. Effects on feeding, larvicidal and pupicidal activities and larval-pupal duration were studied. Higher antifeedant activity (56.06%), lethal concentration for feeding inhibition (3.69%), larvicidal (40.66%), pupicidal (28%), total mortality (68.66%) and prolonged larval-pupal duration (12.04-13.08 days) were observed in ethyl acetate leaf extract at 5.0% concentration. Dose dependant effect of test extracts was observed. This plant could be used to isolate active principles and to develop a new botanical formulation in pest management programmes.
Asian Pacific Journal of Tropical Biomedicine, 2012
To evaluate the efficacy of Atalantia monophylla (A. monophylla) leaf in different solvent crude ... more To evaluate the efficacy of Atalantia monophylla (A. monophylla) leaf in different solvent crude extracts and fractions against eggs of Spodoptera litura (S. litura). Hexane, ethyl acetate and chloroform solvent extracts of A. monophylla leaf and 12 fractions from hexane extract were screened at 5.0%, 2.5%, 1.0% and 0.5% for crude extracts and 1 000, 500, 250 and 125 mg/kg for fractions against the eggs of S. litura for the ovicidal activity. LC50 and LC90 were calculated using probit analysis. Hexane crude extract showed maximum ovicidal activity of 61.94% at 5.0% concentration with a correlation value of r (2)=0.81, and least LC50 value of 3.06%. Hexane extract was fractionated using silica gel column chromatography and 12 fractions were obtained. Fraction 9 was active which showed maximum ovicidal activity of 75.61% at 1 000 mg/kg with the LC50 value of 318.65 mg/kg and LC90 value of 1 473.31 mg/kg. In linear regression analysis, significant and high correlation (r (2)=0.81%) was seen between concentration and ovicidal activity of hexane crude extracts and its active fraction. As per our knowledge, this is the first report for ovicidal activity of A. monophylla against S. litura, A. monophylla could be used for the management of S. litura and other insect pests.
ABSTRACT Fractions derived from Clerodendrum phlomidis Linn. F. and isolated compounds from chlor... more ABSTRACT Fractions derived from Clerodendrum phlomidis Linn. F. and isolated compounds from chloroform extract were evaluated against Earias vittella Fab. for their ovicidal and oviposition deterrent activities. There were 12 fractions obtained with silica gel column chromatographic techniques using different solvent systems. Among the fractions, fraction 5 recorded maximum ovicidal activity of 68.49% and oviposition deterrent activity of 100% at 1000 ppm concentration followed by 500 ppm concentration. The active fraction was further purified using HPLC. The purified fraction was subjected to UV, 1HNMR, 13CNMR, IR and EI-Mass spectroscopy and their spectral data confirmed the compound pectolinaringenin which belongs to the flavonoids. The compound pectolinaringenin was screened for its ovicidal and oviposition deterrent activities. It showed maximum ovicidal activity of 64.29% and oviposition deterrent activity of 100% at 100 ppm concentration against E. vittella. To the best of our knowledge, this is the first report of fractions and isolated compounds from C. phlomidis evaluated against E. vittella for their ovicidal and oviposition deterrent activities. The results from the present investigation clearly suggest that the active fraction or the isolated compound could be used to develop a novel pesticidal formulation to control economically important agricultural pests.
Uploads