Journal of Toxicology and Environmental Health, 1986
Reproductive problems, including congenital malformations, reduced hatching success, and decrease... more Reproductive problems, including congenital malformations, reduced hatching success, and decreased survival of hatchlings, have been observed in colonial-nesting water birds at the San Francisco Bay National Wildlife Refuge (SFBNWR). Twenty-four black-crowned night heron (Nycticorax nycticorax) eggs were collected from SFBNWR in 1983. Twelve of these were collected from separate nests when late-stage embryos were pipping, and an additional egg was randomly collected from each nest for organochlorine analysis. Overt anomalies and skeletal defects were not apparent. Embryonic weights (with partially absorbed yolk sacs removed) were 15% lower (p less than 0.05) in SFBNWR embryos compared to control embryos from the Patuxent Wildlife Research Center (PWRC). Crown-rump length and femur length were shorter for SFBNWR embryos. The geometric mean polychlorinated biphenyl (PCB) concentration in SFBNWR eggs was 4.1 ppm wet weight, with a range of 0.8-52.0 ppm. A negative correlation (r = -0.61; p less than 0.05) existed between embryonic weight and log-transformed PCB residues in whole eggs collected from the same nest at SFBNWR, suggesting a possible impact of PCBs on embryonic growth. A correlation with embryonic weight did not occur for DDE [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene] residues. Liver microsomal aryl hydrocarbon hydroxylase activity was neither significantly elevated nor correlated with PCB, DDE, or PCB plus DDE log-transformed residues. It is unknown whether the apparent association between PCBs and lower weight is persistent through hatching.
Unmarked Double-crested Cormorants (Phalacrocorax auritus) (n = 523) were followed by airplane fr... more Unmarked Double-crested Cormorants (Phalacrocorax auritus) (n = 523) were followed by airplane from Cat Island and Spider Island, two nesting colonies in Wisconsin, to their first landing site. Cormorants flew an average of 2.0 km from Cat Island (maximum 40 km) and 2.4 km from Spider Island (maximum 12 km). The mean direction of landing sites differed seasonally for flights
Journal of toxicology and environmental health, 1991
Two-year-old male mallards (Anas platyrhynchos) received a control diet (0.2 ppm Se) or diets con... more Two-year-old male mallards (Anas platyrhynchos) received a control diet (0.2 ppm Se) or diets containing 1, 2, 4, 8, 16, or 32 ppm Se as selenomethionine for 14 wk. Se accumulated readily in the liver in a dose-dependent manner, reaching a mean concentration of 29 ppm (wet weight) in the 32 ppm group. Dietary Se of 2 ppm or greater increased plasma glutathione peroxidase activity. Mortality (10%) and histopathological effects, including bile duct hyperplasia and hemosiderin pigmentation of the liver and spleen, occurred in the 32 ppm group. These histopathological effects were accompanied by lower hemoglobin concentrations (16 and 32 ppm groups) and hematocrit (32 ppm group), and elevated plasma alkaline phosphatase activity (32 ppm group) indicative of cholestatic liver injury. Other manifestations of hepatotoxicity included significant linear dose responses for hepatic oxidized glutathione (GSSG) concentrations and ratio of GSSG to reduced glutathione (GSH). Means for both of thes...
Journal of toxicology and environmental health, 1991
Beginning the day after hatching, American kestrel (Falco sparverius) nestlings were orally dosed... more Beginning the day after hatching, American kestrel (Falco sparverius) nestlings were orally dosed for 10 consecutive days with 5 microliters/g of corn oil (controls) or one of the diphenyl ether herbicides (nitrofen, bifenox, or oxyfluorfen) at concentrations of 10, 50, 250, or 500 mg/kg in corn oil. At 500 mg/kg, nitrofen resulted in complete nestling mortality, bifenox in high (66%) mortality, and oxyfluorfen in no mortality. Nitrofen at 250 mg/kg reduced nestling growth as reflected by decreased body weight, crown-rump length, and bone lengths including humerus, radius-ulna, femur, and tibiotarsus. Bifenox at 250 mg/kg had less effect on growth than nitrofen, but crown-rump, humerus, radius-ulna, and femur were significantly shorter than controls. Liver weight as a percent of body weight increased with 50 and 250 mg/kg nitrofen. Other manifestations of impending hepatotoxicity following nitrofen ingestion included increased hepatic GSH peroxidase activity in all nitrofen-treated ...
Archives of Environmental Contamination and Toxicology, 1985
One-day old American Kestrel (Falco sparverius) nestlings were dosed orally daily with 5 µl/g of ... more One-day old American Kestrel (Falco sparverius) nestlings were dosed orally daily with 5 µl/g of corn oil (controls), 25 mg/kg, 125 mg/kg, or 625 mg/kg of metallic lead in corn oil through day 10. Forty percent of the nestlings given 625 mg/kg died after six days. Growth rates became significantly different from controls in the 625 mg/kg group by day
Journal of Toxicology and Environmental Health, 1986
Reproductive problems, including congenital malformations, reduced hatching success, and decrease... more Reproductive problems, including congenital malformations, reduced hatching success, and decreased survival of hatchlings, have been observed in colonial-nesting water birds at the San Francisco Bay National Wildlife Refuge (SFBNWR). Twenty-four black-crowned night heron (Nycticorax nycticorax) eggs were collected from SFBNWR in 1983. Twelve of these were collected from separate nests when late-stage embryos were pipping, and an additional egg was randomly collected from each nest for organochlorine analysis. Overt anomalies and skeletal defects were not apparent. Embryonic weights (with partially absorbed yolk sacs removed) were 15% lower (p less than 0.05) in SFBNWR embryos compared to control embryos from the Patuxent Wildlife Research Center (PWRC). Crown-rump length and femur length were shorter for SFBNWR embryos. The geometric mean polychlorinated biphenyl (PCB) concentration in SFBNWR eggs was 4.1 ppm wet weight, with a range of 0.8-52.0 ppm. A negative correlation (r = -0.61; p less than 0.05) existed between embryonic weight and log-transformed PCB residues in whole eggs collected from the same nest at SFBNWR, suggesting a possible impact of PCBs on embryonic growth. A correlation with embryonic weight did not occur for DDE [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene] residues. Liver microsomal aryl hydrocarbon hydroxylase activity was neither significantly elevated nor correlated with PCB, DDE, or PCB plus DDE log-transformed residues. It is unknown whether the apparent association between PCBs and lower weight is persistent through hatching.
Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphat... more Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine phosphokinase (CPK), and lactate dehydrogenase (LDH) were determined in plasma, kidney, liver, and muscle from five species of captive birds. Few differences occurred in plasma activities between sexes but considerable differences occurred between species. All five enzymes were detected in each of the tissues sampled. Relative enzyme activities in liver, kidney, and muscle were similar for each species. CPK activity was much higher in muscle than in liver or kidney and, of the five enzymes studied, may be the best indicator of muscle damage. Most of the other enzymes were more evenly distributed among the three tissues, and no organ-specific enzyme could be identified for liver or kidney. Because of interspecific variations in plasma enzyme activities, it is important to establish baseline values for each species to ensure accurate interpretation of results.
In winter, pen-reared and wild black ducks (Anas rubripes), and game farm and wild mallards (Anas... more In winter, pen-reared and wild black ducks (Anas rubripes), and game farm and wild mallards (Anas platyrhynchos), maintained on pelleted feed, were sham-dosed or given one number 4 lead shot. After 14 days, dosed birds were redosed with two or four additional lead shot. This dosing regimen also was repeated in summer using pen-reared black ducks and game farm mallards. Based upon mortality, overt intoxication, weight change, delta-aminolevulinic acid dehydratase activity and protoporphyrin concentration, black ducks and mallards were found to be equally tolerant to lead shot. However, captive wild ducks were more sensitive than their domesticated counterparts, as evidenced by greater mortality and weight loss following lead shot administration. This difference may be related to stress associated with captivity and unnatural diet.
Starlings were collected for the National Pesticides Monitoring Program from 112 sites throughout... more Starlings were collected for the National Pesticides Monitoring Program from 112 sites throughout the contiguous United States and analyzed for organochlorine compounds. Starling samples from 14 sites had greater than 1.0 ppm DDE residues with the highest DDE level being 15.8 ppm in a sample taken near Roswell, New Mexico. The occurrence of PCBs and chlordane isomers increased since the 1976 collection, but DDT, DDE, and dieldrin occurrence decreased. DDE and dieldrin levels decreased since the 1976 collection, but the level of PCBs has increased.
Bulletin of Environmental Contamination and Toxicology, 1985
... We thank Paula Decker and Jeanne Grillo for preparation of the figures; Kathy Klimkiewicz and... more ... We thank Paula Decker and Jeanne Grillo for preparation of the figures; Kathy Klimkiewicz and Stan Wiemeyer for reviewing the manuscript. ... 5:95-103 Mendenhall VM, Klaas EE, McLane MAR (1983) Breeding success of barn owls (Tyto alba) fed low levels of DDE and dieldrin. ...
A survey conducted in 1995 investigated long term declines reported in a population of box turtle... more A survey conducted in 1995 investigated long term declines reported in a population of box turtles Terrapene carolina monitored each decade since 1945 in bottomland hardwood forest at the Patuxent Wildlife Research Center, Maryland. Methods duplicated past surveys in most respects, but were supplemented by radiotelemetry and a survey of dominant vegetation. Seventy different turtles were found on the 11.8
Archives of Environmental Contamination and Toxicology, 1993
Bald eagle (Haliaeetus leucocephalus) eggs were collected in 15 States in the United States in 19... more Bald eagle (Haliaeetus leucocephalus) eggs were collected in 15 States in the United States in 19801984 and analyzed for organochlorine pesticides, polychlorinated biphenyls (PCBs), and mercury. Data were compared and combined with data from earlier studies to ...
Journal of Toxicology and Environmental Health, 1986
Reproductive problems, including congenital malformations, reduced hatching success, and decrease... more Reproductive problems, including congenital malformations, reduced hatching success, and decreased survival of hatchlings, have been observed in colonial-nesting water birds at the San Francisco Bay National Wildlife Refuge (SFBNWR). Twenty-four black-crowned night heron (Nycticorax nycticorax) eggs were collected from SFBNWR in 1983. Twelve of these were collected from separate nests when late-stage embryos were pipping, and an additional egg was randomly collected from each nest for organochlorine analysis. Overt anomalies and skeletal defects were not apparent. Embryonic weights (with partially absorbed yolk sacs removed) were 15% lower (p less than 0.05) in SFBNWR embryos compared to control embryos from the Patuxent Wildlife Research Center (PWRC). Crown-rump length and femur length were shorter for SFBNWR embryos. The geometric mean polychlorinated biphenyl (PCB) concentration in SFBNWR eggs was 4.1 ppm wet weight, with a range of 0.8-52.0 ppm. A negative correlation (r = -0.61; p less than 0.05) existed between embryonic weight and log-transformed PCB residues in whole eggs collected from the same nest at SFBNWR, suggesting a possible impact of PCBs on embryonic growth. A correlation with embryonic weight did not occur for DDE [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene] residues. Liver microsomal aryl hydrocarbon hydroxylase activity was neither significantly elevated nor correlated with PCB, DDE, or PCB plus DDE log-transformed residues. It is unknown whether the apparent association between PCBs and lower weight is persistent through hatching.
Unmarked Double-crested Cormorants (Phalacrocorax auritus) (n = 523) were followed by airplane fr... more Unmarked Double-crested Cormorants (Phalacrocorax auritus) (n = 523) were followed by airplane from Cat Island and Spider Island, two nesting colonies in Wisconsin, to their first landing site. Cormorants flew an average of 2.0 km from Cat Island (maximum 40 km) and 2.4 km from Spider Island (maximum 12 km). The mean direction of landing sites differed seasonally for flights
Journal of toxicology and environmental health, 1991
Two-year-old male mallards (Anas platyrhynchos) received a control diet (0.2 ppm Se) or diets con... more Two-year-old male mallards (Anas platyrhynchos) received a control diet (0.2 ppm Se) or diets containing 1, 2, 4, 8, 16, or 32 ppm Se as selenomethionine for 14 wk. Se accumulated readily in the liver in a dose-dependent manner, reaching a mean concentration of 29 ppm (wet weight) in the 32 ppm group. Dietary Se of 2 ppm or greater increased plasma glutathione peroxidase activity. Mortality (10%) and histopathological effects, including bile duct hyperplasia and hemosiderin pigmentation of the liver and spleen, occurred in the 32 ppm group. These histopathological effects were accompanied by lower hemoglobin concentrations (16 and 32 ppm groups) and hematocrit (32 ppm group), and elevated plasma alkaline phosphatase activity (32 ppm group) indicative of cholestatic liver injury. Other manifestations of hepatotoxicity included significant linear dose responses for hepatic oxidized glutathione (GSSG) concentrations and ratio of GSSG to reduced glutathione (GSH). Means for both of thes...
Journal of toxicology and environmental health, 1991
Beginning the day after hatching, American kestrel (Falco sparverius) nestlings were orally dosed... more Beginning the day after hatching, American kestrel (Falco sparverius) nestlings were orally dosed for 10 consecutive days with 5 microliters/g of corn oil (controls) or one of the diphenyl ether herbicides (nitrofen, bifenox, or oxyfluorfen) at concentrations of 10, 50, 250, or 500 mg/kg in corn oil. At 500 mg/kg, nitrofen resulted in complete nestling mortality, bifenox in high (66%) mortality, and oxyfluorfen in no mortality. Nitrofen at 250 mg/kg reduced nestling growth as reflected by decreased body weight, crown-rump length, and bone lengths including humerus, radius-ulna, femur, and tibiotarsus. Bifenox at 250 mg/kg had less effect on growth than nitrofen, but crown-rump, humerus, radius-ulna, and femur were significantly shorter than controls. Liver weight as a percent of body weight increased with 50 and 250 mg/kg nitrofen. Other manifestations of impending hepatotoxicity following nitrofen ingestion included increased hepatic GSH peroxidase activity in all nitrofen-treated ...
Archives of Environmental Contamination and Toxicology, 1985
One-day old American Kestrel (Falco sparverius) nestlings were dosed orally daily with 5 µl/g of ... more One-day old American Kestrel (Falco sparverius) nestlings were dosed orally daily with 5 µl/g of corn oil (controls), 25 mg/kg, 125 mg/kg, or 625 mg/kg of metallic lead in corn oil through day 10. Forty percent of the nestlings given 625 mg/kg died after six days. Growth rates became significantly different from controls in the 625 mg/kg group by day
Journal of Toxicology and Environmental Health, 1986
Reproductive problems, including congenital malformations, reduced hatching success, and decrease... more Reproductive problems, including congenital malformations, reduced hatching success, and decreased survival of hatchlings, have been observed in colonial-nesting water birds at the San Francisco Bay National Wildlife Refuge (SFBNWR). Twenty-four black-crowned night heron (Nycticorax nycticorax) eggs were collected from SFBNWR in 1983. Twelve of these were collected from separate nests when late-stage embryos were pipping, and an additional egg was randomly collected from each nest for organochlorine analysis. Overt anomalies and skeletal defects were not apparent. Embryonic weights (with partially absorbed yolk sacs removed) were 15% lower (p less than 0.05) in SFBNWR embryos compared to control embryos from the Patuxent Wildlife Research Center (PWRC). Crown-rump length and femur length were shorter for SFBNWR embryos. The geometric mean polychlorinated biphenyl (PCB) concentration in SFBNWR eggs was 4.1 ppm wet weight, with a range of 0.8-52.0 ppm. A negative correlation (r = -0.61; p less than 0.05) existed between embryonic weight and log-transformed PCB residues in whole eggs collected from the same nest at SFBNWR, suggesting a possible impact of PCBs on embryonic growth. A correlation with embryonic weight did not occur for DDE [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene] residues. Liver microsomal aryl hydrocarbon hydroxylase activity was neither significantly elevated nor correlated with PCB, DDE, or PCB plus DDE log-transformed residues. It is unknown whether the apparent association between PCBs and lower weight is persistent through hatching.
Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphat... more Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine phosphokinase (CPK), and lactate dehydrogenase (LDH) were determined in plasma, kidney, liver, and muscle from five species of captive birds. Few differences occurred in plasma activities between sexes but considerable differences occurred between species. All five enzymes were detected in each of the tissues sampled. Relative enzyme activities in liver, kidney, and muscle were similar for each species. CPK activity was much higher in muscle than in liver or kidney and, of the five enzymes studied, may be the best indicator of muscle damage. Most of the other enzymes were more evenly distributed among the three tissues, and no organ-specific enzyme could be identified for liver or kidney. Because of interspecific variations in plasma enzyme activities, it is important to establish baseline values for each species to ensure accurate interpretation of results.
In winter, pen-reared and wild black ducks (Anas rubripes), and game farm and wild mallards (Anas... more In winter, pen-reared and wild black ducks (Anas rubripes), and game farm and wild mallards (Anas platyrhynchos), maintained on pelleted feed, were sham-dosed or given one number 4 lead shot. After 14 days, dosed birds were redosed with two or four additional lead shot. This dosing regimen also was repeated in summer using pen-reared black ducks and game farm mallards. Based upon mortality, overt intoxication, weight change, delta-aminolevulinic acid dehydratase activity and protoporphyrin concentration, black ducks and mallards were found to be equally tolerant to lead shot. However, captive wild ducks were more sensitive than their domesticated counterparts, as evidenced by greater mortality and weight loss following lead shot administration. This difference may be related to stress associated with captivity and unnatural diet.
Starlings were collected for the National Pesticides Monitoring Program from 112 sites throughout... more Starlings were collected for the National Pesticides Monitoring Program from 112 sites throughout the contiguous United States and analyzed for organochlorine compounds. Starling samples from 14 sites had greater than 1.0 ppm DDE residues with the highest DDE level being 15.8 ppm in a sample taken near Roswell, New Mexico. The occurrence of PCBs and chlordane isomers increased since the 1976 collection, but DDT, DDE, and dieldrin occurrence decreased. DDE and dieldrin levels decreased since the 1976 collection, but the level of PCBs has increased.
Bulletin of Environmental Contamination and Toxicology, 1985
... We thank Paula Decker and Jeanne Grillo for preparation of the figures; Kathy Klimkiewicz and... more ... We thank Paula Decker and Jeanne Grillo for preparation of the figures; Kathy Klimkiewicz and Stan Wiemeyer for reviewing the manuscript. ... 5:95-103 Mendenhall VM, Klaas EE, McLane MAR (1983) Breeding success of barn owls (Tyto alba) fed low levels of DDE and dieldrin. ...
A survey conducted in 1995 investigated long term declines reported in a population of box turtle... more A survey conducted in 1995 investigated long term declines reported in a population of box turtles Terrapene carolina monitored each decade since 1945 in bottomland hardwood forest at the Patuxent Wildlife Research Center, Maryland. Methods duplicated past surveys in most respects, but were supplemented by radiotelemetry and a survey of dominant vegetation. Seventy different turtles were found on the 11.8
Archives of Environmental Contamination and Toxicology, 1993
Bald eagle (Haliaeetus leucocephalus) eggs were collected in 15 States in the United States in 19... more Bald eagle (Haliaeetus leucocephalus) eggs were collected in 15 States in the United States in 19801984 and analyzed for organochlorine pesticides, polychlorinated biphenyls (PCBs), and mercury. Data were compared and combined with data from earlier studies to ...
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