Current Opinion in Investigational Drugs, Feb 1, 2002
Recent studies have shown that long-lived memory CD8 T-cell populations can be subdivided into tw... more Recent studies have shown that long-lived memory CD8 T-cell populations can be subdivided into two distinct groups. The central pool of resting memory cells in lymph organs has been extensively analyzed. However, a second subset of partially activated CD8 memory T-cells has recently been identified in non-lymphoid tissues. In respiratory virus models, a strong correlation between the numbers of antigen-specific T-cells in lung tissues and effective protective cellular immunity suggests that boosting the numbers of memory T-cells in non-lymphoid tissues may be a fruitful approach in vaccine development for viral infections.
Proceedings of the American Thoracic Society, 2005
The respiratory tract poses a substantial challenge to the immune system due to its large surface... more The respiratory tract poses a substantial challenge to the immune system due to its large surface area, an extensive vasculature that is in very close proximity to the external environment, and repeated exposure to potentially pathogenic organisms in the air. Yet many lung pathogens are controlled by appropriate immune responses. The underlying mechanisms of the adaptive cellular immune response in protecting the respiratory tract are poorly understood. Recently, it has emerged that memory CD4(+) and CD8(+) T cells are present in the lung airways, and evidence is mounting that these cells play a key role in pulmonary immunity to pathogen challenge by immediately engaging the pathogen at the site of infection when pathogen loads are low. For example, in the case of respiratory virus infections, there is evidence that both CD4(+) and CD8(+) memory cells in the lung airways mediate substantial control of a secondary respiratory virus infection in the lungs. Here we address recent devel...
We have used T-cell receptor beta-chain transgenic mice to determine the effects of a limited T-c... more We have used T-cell receptor beta-chain transgenic mice to determine the effects of a limited T-cell receptor repertoire on major histocompatibility complex class I-restricted epitope selection during the course of an influenza virus infection. Analysis of T-cell hybridomas generated from wild-type and transgenic mice demonstrated that the viral epitope recognized depended on the available T-cell receptor repertoire. Wild-type T-cell hybridomas recognized epitopes derived from the nucleoprotein and basic polymerase molecules, whereas hybridomas generated from transgenic mice recognized epitopes derived from the nonstructural protein and the matrix protein. There was no overlap in specificity between the two panels of hybridomas. This reciprocal pattern of specificity was also apparent in cytoxicity assays with brochoalveolar lavage cells isolated from the lungs of influenza virus-infected mice. T-cell receptor usage in the transgenic hybridomas was very restricted, with only one V a...
Vaccination with live attenuated influenza A virus (LAIV) has the potential to provide protection... more Vaccination with live attenuated influenza A virus (LAIV) has the potential to provide protection against the more severe consequences of pandemic flu regardless of subtype. This possibility should be explored.
New approaches to visualizing antigen-specific primary responses to influenza and the development... more New approaches to visualizing antigen-specific primary responses to influenza and the development of memory subsets in distinct sites suggest that both CD4 and CD8 T cells play complex roles in primary viral clearance and have the potential to contribute to protection from secondary infection.
Current Opinion in Investigational Drugs, Feb 1, 2002
Recent studies have shown that long-lived memory CD8 T-cell populations can be subdivided into tw... more Recent studies have shown that long-lived memory CD8 T-cell populations can be subdivided into two distinct groups. The central pool of resting memory cells in lymph organs has been extensively analyzed. However, a second subset of partially activated CD8 memory T-cells has recently been identified in non-lymphoid tissues. In respiratory virus models, a strong correlation between the numbers of antigen-specific T-cells in lung tissues and effective protective cellular immunity suggests that boosting the numbers of memory T-cells in non-lymphoid tissues may be a fruitful approach in vaccine development for viral infections.
Proceedings of the American Thoracic Society, 2005
The respiratory tract poses a substantial challenge to the immune system due to its large surface... more The respiratory tract poses a substantial challenge to the immune system due to its large surface area, an extensive vasculature that is in very close proximity to the external environment, and repeated exposure to potentially pathogenic organisms in the air. Yet many lung pathogens are controlled by appropriate immune responses. The underlying mechanisms of the adaptive cellular immune response in protecting the respiratory tract are poorly understood. Recently, it has emerged that memory CD4(+) and CD8(+) T cells are present in the lung airways, and evidence is mounting that these cells play a key role in pulmonary immunity to pathogen challenge by immediately engaging the pathogen at the site of infection when pathogen loads are low. For example, in the case of respiratory virus infections, there is evidence that both CD4(+) and CD8(+) memory cells in the lung airways mediate substantial control of a secondary respiratory virus infection in the lungs. Here we address recent devel...
We have used T-cell receptor beta-chain transgenic mice to determine the effects of a limited T-c... more We have used T-cell receptor beta-chain transgenic mice to determine the effects of a limited T-cell receptor repertoire on major histocompatibility complex class I-restricted epitope selection during the course of an influenza virus infection. Analysis of T-cell hybridomas generated from wild-type and transgenic mice demonstrated that the viral epitope recognized depended on the available T-cell receptor repertoire. Wild-type T-cell hybridomas recognized epitopes derived from the nucleoprotein and basic polymerase molecules, whereas hybridomas generated from transgenic mice recognized epitopes derived from the nonstructural protein and the matrix protein. There was no overlap in specificity between the two panels of hybridomas. This reciprocal pattern of specificity was also apparent in cytoxicity assays with brochoalveolar lavage cells isolated from the lungs of influenza virus-infected mice. T-cell receptor usage in the transgenic hybridomas was very restricted, with only one V a...
Vaccination with live attenuated influenza A virus (LAIV) has the potential to provide protection... more Vaccination with live attenuated influenza A virus (LAIV) has the potential to provide protection against the more severe consequences of pandemic flu regardless of subtype. This possibility should be explored.
New approaches to visualizing antigen-specific primary responses to influenza and the development... more New approaches to visualizing antigen-specific primary responses to influenza and the development of memory subsets in distinct sites suggest that both CD4 and CD8 T cells play complex roles in primary viral clearance and have the potential to contribute to protection from secondary infection.
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Papers by David Woodland