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... Record Details. Record ID, 902100. Record Type, conference. Author, Katelijn Schautteet [802000077535] - Ghent University Katelijn.Schautteet@UGent.be; Delphine Sylvie Anne Beeckman [801002018970] - Ghent University... more
... Record Details. Record ID, 902100. Record Type, conference. Author, Katelijn Schautteet [802000077535] - Ghent University Katelijn.Schautteet@UGent.be; Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@UGent.be; ...
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... Record ID, 1036053. Record Type, conference. Author, Caroline Van Droogenbroeck [801002092530] - Ghent University Caroline.VanDroogenbroeck@UGent.be; Delphine Sylvie Anne Beeckman [801002018970] - Ghent University... more
... Record ID, 1036053. Record Type, conference. Author, Caroline Van Droogenbroeck [801002092530] - Ghent University Caroline.VanDroogenbroeck@UGent.be; Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@UGent.be; ...
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... Record Details. Record ID, 376805. Record Type, conference. Author, Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@UGent.be; Patric Van Oostveldt [801000231039] - Ghent University... more
... Record Details. Record ID, 376805. Record Type, conference. Author, Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@UGent.be; Patric Van Oostveldt [801000231039] - Ghent University Patrick.VanOostveldt@UGent.be; ...
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Additional file 8: Fig. S6. Summary of Gubbins recombination analysis including avian C. abortus strains 15-59d/3 and 15-70d/24. The red blocks represent recombination events occurring on an internal branch of the phylogenetic tree, which... more
Additional file 8: Fig. S6. Summary of Gubbins recombination analysis including avian C. abortus strains 15-59d/3 and 15-70d/24. The red blocks represent recombination events occurring on an internal branch of the phylogenetic tree, which are shared by several strains by common descent. The blue blocks indicate recombination events occurring on terminal branches of the phylogenetic tree, which are unique to a specific strain. The parameters used for the run are those described in Fig. 4.
Additional file 6: Fig. S4. Whole genome phylogenetic analysis. Phylogenetic tree of a whole genome sequence MAFFT alignment of the C. abortus (Cab) and C. psittaci (Cps) strains shown in Table 2, and C. buteonis strain RSHA. The... more
Additional file 6: Fig. S4. Whole genome phylogenetic analysis. Phylogenetic tree of a whole genome sequence MAFFT alignment of the C. abortus (Cab) and C. psittaci (Cps) strains shown in Table 2, and C. buteonis strain RSHA. The consensus tree was estimated in IQ-Tree by Maximum Likelihood using a TVM + F + R2 substitution and rate heterogeneity model, according to BIC model selection, and 100 non-parametric bootstrap replicates. The tree is midpoint rooted and bootstrap support is indicated by the number at the node. The scale bar indicates the expected substitutions per site. Genotypes are given in square brackets. The tree was prepared in Dendroscope. Strain 84/2334 is in bold and red font. Classical and avian C. abortus strains are in blue and green fonts, respectively.
Additional file 7: Fig. S5. Whole genome NeighborNet network analysis. Phylogenetic network of a whole genome sequence alignment of the C. abortus (Cab) and C. psittaci (Cps) strains shown in Table 2, using the NeighborNet distances... more
Additional file 7: Fig. S5. Whole genome NeighborNet network analysis. Phylogenetic network of a whole genome sequence alignment of the C. abortus (Cab) and C. psittaci (Cps) strains shown in Table 2, using the NeighborNet distances transformation (Ordinary Least Squares variance and Lambda Frac of 1.0) and EqualAngle splits transformation. The scale bar indicates the expected substitutions per site. Genotypes of C. psittaci and avian C. abortus strains are indicated in brackets. The figure was generated using SplitsTree4. Strain 84/2334 is in bold and red font.
Additional file 3: Fig. S2. Phylogenetic tree of a 16S rRNA gene alignment of strain 84/2334 and other Chlamydiaceae species. The consensus tree for the 1470 bp alignment was estimated in TOPALi by Neighbour Joining using a F84 + G... more
Additional file 3: Fig. S2. Phylogenetic tree of a 16S rRNA gene alignment of strain 84/2334 and other Chlamydiaceae species. The consensus tree for the 1470 bp alignment was estimated in TOPALi by Neighbour Joining using a F84 + G substitution and rate heterogeneity model and 100 non-parametric bootstrap replicates. The tree is midpoint rooted and bootstrap support is indicated by the number at the node. The scale bar indicates the expected substitutions per site. Genotypes are given in square brackets. The tree was prepared in Dendroscope. Strain 84/2334 is in bold and red font.
Additional file 11: Fig. S9. Polymorphic membrane protein family phylogenies. Consensus trees for singleton PmpA, PmpB, PmpD and PmpH family members (A), for the PmpE family members (B) and PmpG family members (C) for strain 84/2334 and... more
Additional file 11: Fig. S9. Polymorphic membrane protein family phylogenies. Consensus trees for singleton PmpA, PmpB, PmpD and PmpH family members (A), for the PmpE family members (B) and PmpG family members (C) for strain 84/2334 and the C. abortus and C. psittaci strains/genotypes shown in Table 2 were estimated in IQ-Tree by Maximum Likelihood using substitution and rate heterogeneity models JTTDCMut + F + I + G4, JTT + F + R3 and JTT + F + R5, respectively, according to BIC model selection, and 100 non-parametric bootstrap replicates. The trees were midpoint rooted and bootstrap support is indicated by the number at the node (only values greater than 70 are shown). The scale bar indicates the expected substitutions per site. The trees were prepared in Dendroscope. Strain 84/2334 is in bold and red font. Classical and avian C. abortus strains are in blue and green fonts, respectively.
Research Interests: Epidemiology and Biology
Traditionally, the amount of infective chlamydiae in a given sample is determined by inoculating dilution series into cell cultures and physically counting chlamydial inclusions. This approach is time consuming, tedious, and error prone,... more
Traditionally, the amount of infective chlamydiae in a given sample is determined by inoculating dilution series into cell cultures and physically counting chlamydial inclusions. This approach is time consuming, tedious, and error prone, mainly when dealing with high titers. Therefore, this paper describes a largely automated technique that was developed to standardize the determination of chlamydial load in vitro. Cells are fixed at 36 h post-inoculation and bacteria visualized using standard immunological detection methods. Consequently, for 81 microscopic fields, an image is recorded at the interpolated focal plane. These images are then automatically processed using an ImageJ plugin and the obtained results are imported into Excel to determine the number of inclusion forming units per mL in the sample. The main advantage of this technique is that no or minimal sample dilution is required, thus minimizing dilution errors. In addition, this technique was employed during the early, middle and late growth stages of the chlamydial developmental cycle and results correlated well (P < 0.01) with 16S rRNA values from previous experiments, thereby proving its suitability to follow chlamydial growth in vitro. The method described is highly suitable for high throughput titration of cell culture inoculated samples and assessment of possible antichlamydial effects of novel compounds throughout the chlamydial growth cycle.
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Copyright It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open... more
Copyright It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content licence (like
Research Interests: Biology, Medicine, Multidisciplinary, Chlamydia trachomatis, Chlamydia pneumoniae, and 14 moreDNA, Phylogeny, Population structure, Pregnancy, Humans, Female, Animals, Polymerase Chain Reaction, PLoS one, Genotype, Species Specificity, Respiratory Tract Infection, Fetal death, and Likelihood Functions
... UGent.be; Delphine Sylvie Anne Beeckman [801002018970] - Ghent UniversityDelphine.Beeckman@UGent.be; Marian Carlon; S Van Acker; Koen Chiers [801001013810] - Ghent University Koen.Chiers@UGent.be; Eric Cox ...
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Research Interests:
... UGent.be; Delphine Sylvie Anne Beeckman [801002018970] - Ghent UniversityDelphine.Beeckman@UGent.be; Marianne Carlon; Sofie Van Acker; Koen Chiers [801001013810] - Ghent University Koen.Chiers@UGent.be; Eric ...
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Chlamydia trachomatis is a bacterial pathogen that is the leading cause of bacterial “Sexual Transmitted Disease” (STD) in developing countries. Most often the infection is asymptomatic. However, if the infection remains untreated, it... more
Chlamydia trachomatis is a bacterial pathogen that is the leading cause of bacterial “Sexual Transmitted Disease” (STD) in developing countries. Most often the infection is asymptomatic. However, if the infection remains untreated, it often results in pelvic inflammatory disease (PID), ectopic pregnancy, chronic pelvic pain in women, urethritis and epididymitis in men or infant pneumonia. The infection can easily be treated with antibiotics, but in most cases damage is already done before the bacterium is noticed. Immunization is considered to be the best approach to reduce C. trachomatis infections. However, so far no vaccine is available. In this study, plasmid DNA (pWRG7079::MOMP) expressing the major outer membrane protein of a human Chlamydia trachomatis serovar E strain was tested for the ability to induce an immune response and protect against experimental genital infection with the same serovar. The vaccine was tested in pigs, as they are genetically, physiologically and imm...
Introduction: Chlamydophila psittaci is an important poultry pathogen, especially in turkeys and ducks, causing infections of mucosal epithelial cells and macrophages of the respiratory tract, followed by septicaemia and localisation in... more
Introduction: Chlamydophila psittaci is an important poultry pathogen, especially in turkeys and ducks, causing infections of mucosal epithelial cells and macrophages of the respiratory tract, followed by septicaemia and localisation in epithelial cells and macrophages in various organs. Chlamydial infections in poultry not only present significant economical losses, public health is also under consideration since veterinary surgeons and poultry workers are at high risk of becoming infected by this zoonotic agent. Objectives: the effect of ovotransferrin (ovoTF), human lactoferrin (hLF) and bovine lactoferrin (bLF) on the obligate intracellular Gram-negative bacterium Chlamydophila (Cp.) psittaci was evaluated using a model of African Green Monkey kidney (BGM) cells and chicken macrophages (HD11 cells) as artificial hosts. Subsequently, the potential use of ovotransferrin (ovoTF) to prevent chlamydiosis in vivo was evaluated using experimentally infected SPF turkeys. Results: firstl...
Chlamydiaceae are Gram-negative obligate intracellular bacteria and are therefore completely dependent on a living host cell to execute their unique biphasic developmental cycle. This is accompanied by a reprogramming of the host cell... more
Chlamydiaceae are Gram-negative obligate intracellular bacteria and are therefore completely dependent on a living host cell to execute their unique biphasic developmental cycle. This is accompanied by a reprogramming of the host cell into a minimized factory efficiently supporting chlamydial replication. Type III secretion could be essential in this process as other Gram-negative bacteria deploy similar secretion mechanisms to subvert the cellular functions of their host to their own benefit. Chlamydophila psittaci primarily infects birds and is horizontally transmitted through aerosols of nasal secretions and feces. Initially, the respiratory tract is infected, from where the disease further spreads leading to a systemic infection. The commercial poultry industry is affected by considerable economic losses, especially when the animals are simultaneously infected with avian pathogenic E. coli, Ornithobacterium rhinotracheale or avian metapneumovirus. Zoonotic transmission occurs in...
... Ghent University Caroline.VanDroogenbroeck@UGent.be; Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@UGent.be; Eric Cox [801000669458] - Ghent University Eric.Cox@UGent.be; Daisy Vanrompay... more
... Ghent University Caroline.VanDroogenbroeck@UGent.be; Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@UGent.be; Eric Cox [801000669458] - Ghent University Eric.Cox@UGent.be; Daisy Vanrompay [801000900743] - Ghent ...
... Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@ UGent.be; Daisy Vanrompay [801000900743] - Ghent University Daisy.Vanrompay@UGent. be. Title, Chlamydophila psittaci in Belgian wild birds and feral... more
... Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@ UGent.be; Daisy Vanrompay [801000900743] - Ghent University Daisy.Vanrompay@UGent. be. Title, Chlamydophila psittaci in Belgian wild birds and feral pigeons. ...
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Research Interests:
... psittaci. Please use this identifier to cite or link to this item: Record Details. Record ID, 632491. Record Type, dissertation. Author, Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@UGent.be. ...
Numerous bacterial proteins exert their function outside the prokaryotic cell. To this end, both Gram-negative and Gram-positive bacteria have evolved specialized mechanisms to transport their proteins to the bacterial supernatant or host... more
Numerous bacterial proteins exert their function outside the prokaryotic cell. To this end, both Gram-negative and Gram-positive bacteria have evolved specialized mechanisms to transport their proteins to the bacterial supernatant or host cell cytoplasm, so called secretion systems. These different strategies will be briefly discussed, followed by an in depth description of the Type III secretion system, an efficient molecular syringe assisting Gram-negative bacteria in entrance, growth and survival in eukaryotic host cells. Topics addressed include classification and role of multiple Type III secretion systems, the mechanism of protein translocation into the host cell as well as substrate recognition and chaperoning. Chlamydiales have also been found to encode a Type III secretion system and associated effector proteins. In contrast to the genetic organization in other bacteria, the encoding genes are scattered throughout the genome. To date, no structural information is available ...
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Two groups of five 1-day-old conventional turkeys were housed in negative pressure stables to become experimentally infected with Avian Metapneumovirus (aMPV) and Ornithobacterium rhinotracheale (ORT) at the age of 3 weeks. However,... more
Two groups of five 1-day-old conventional turkeys were housed in negative pressure stables to become experimentally infected with Avian Metapneumovirus (aMPV) and Ornithobacterium rhinotracheale (ORT) at the age of 3 weeks. However, during the first 2 weeks, turkeys started to show respiratory disease characterized by rhinitis and dyspnoea. Routine bacterial and viral diagnoses remained negative. Therefore, pharyngeal swabs from the turkeys and from the veterinary scientist handling the animals were examined for the presence of Chlamydophila (C.) psittaci by using a combination of cell culture, nested PCR and ompA genotype-specific quantitative real-time PCR, as well as by serology. Results revealed simultaneous transmission of C. psittaci outer membrane protein A (ompA) genotypes D, F and E/B from infected turkeys to the veterinary scientist.
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Respiratory pathogens are difficult to control in large-scale turkey production. This report describes a clinical trial of antimicrobial ovoTF aerosol on a large Belgian turkey farm. ovoTF was administered to reduce Chlamydia psittaci (C.... more
Respiratory pathogens are difficult to control in large-scale turkey production. This report describes a clinical trial of antimicrobial ovoTF aerosol on a large Belgian turkey farm. ovoTF was administered to reduce Chlamydia psittaci (C. psittaci) infections and to study the impact of this action on the occurrence of Ornithobacterium rhinotracheale (O. rhinotracheale) and avian metapneumovirus (aMPV) infections. Two subsequent broods were included; (i) a control brood receiving no ovoTF and (ii) an ovoTF brood receiving ovoTF aerosol (5mg/animal) at the age of 2 weeks, continuing daily for 12 days. Twenty-four one-day-old toms of the control and ovoTF brood were tagged and monitored for 15 weeks. The control brood experienced two periods of respiratory disease, the first (2-3 weeks of age) due to C. psittaci and the second (8-17 weeks of age) in the presence of C. psittaci, O. rhinotracheale and maybe aMPV. Extensive antibiotic treatment was needed in 2, 8 and 9 week-old toms. In the ovoTF brood, toms stayed healthy until the age of 9 weeks, whereafter respiratory disease occurred in the presence of C. psittaci, O rhinotracheale and aMPV. OvoTF administration: (i) reduced the amount of C. psittaci in the air as demonstrated by bioaerosol monitoring, (ii) prevented respiratory disease during the first half of the brood period, (iii) was associated with 46% reduction of mortality, and (iv) reduced the antibiotic cost. Our results justify additional clinical trials to explore the use of this innovative antimicrobial strategy for poultry.
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Research Interests: Genetics, Microbiology, Electron Microscopy, Veterinary Microbiology, Mitochondria, and 15 moreMacrophages, Veterinary, Cell Division, Cell line, Chicken, Animals, Bacteria, Actin, POULTRY DISEASES, Chickens, Degeneration, Veterinary Sciences, Intracellular Space, Gene Expression Regulation, and Intracellular
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Research Interests: Immune response, Survival Analysis, Pharmaceutical Chemistry, Veterinary, Middle East, and 17 moreBiological Sciences, DNA, Animals, Bacteria, Genetic transformation, Vaccine, DNA vaccines, Vaccination, Codon Usage, DNA vaccination, Experimental Infection, POULTRY DISEASES, DNA vaccine, Codon, P/BV Ratio, Plasmid DNA, and Gene Transfer
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A concurrent outbreak of chlamydial disease in boars, sows and gilts and postweaning multisystemic wasting syndrome (PMWS) in weaned piglets was investigated on a large pig production farm in Estonia. Chlamydia suis DNA was detected in... more
A concurrent outbreak of chlamydial disease in boars, sows and gilts and postweaning multisystemic wasting syndrome (PMWS) in weaned piglets was investigated on a large pig production farm in Estonia. Chlamydia suis DNA was detected in conjunctival swabs from boars, sows and gilts, but also in the faeces of boars and sows. Chlamydophila abortus DNA was found in semen, and in conjunctival swabs from sows; DNA was demonstrated by microarrays. Serum samples from boars were examined using a Chlamydiaceae-specific recombinant ELISA. All 10 serum samples examined were positive (1:960 to 1:3840). Chlamydiosis was characterised by reproductive failure and conjunctivitis. Piglets were not examined for Chlamydiaceae, as eye problems were not observed. Piglets showed wasting, respiratory signs, diarrhoea, enlargement of lymph nodes and increased mortality (10 per cent). Porcine circovirus type 2 (PCV-2) was detected in the lymph nodes of piglets by immunohistochemistry, and PCV-2 antibodies were demonstrated in all 10 serum samples from sows examined using an immunoperoxidase monolayer assay.
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Research Interests: Immunology, Cytokines, Macrophages, Innate immunity, Virulence, and 17 moreCell line, Respiratory Disease, Escherichia coli, Animals, Developmental, Lipopolysaccharides, Genotype, Chickens, Cell Proliferation, Species Specificity, qRT PCR, APV, E, C, Gene expression profiling, Hybridomas, and Inflammatory response
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... psittaci. Please use this identifier to cite or link to this item: Record Details. Record ID, 632491. Record Type, dissertation. Author, Delphine Sylvie Anne Beeckman [801002018970] - Ghent University Delphine.Beeckman@UGent.be. ...