O DN e utilizado no intuito de aumento da massa muscular, ganho de forca, e consequentemente melhorar o desempenho fisico e atletico. Segundo Frankenfeld et al. (2014) as doses utilizadas sao de 10 a 100 vezes maiores que a dose... more
O DN e utilizado no intuito de aumento da massa muscular, ganho de forca, e consequentemente melhorar o desempenho fisico e atletico. Segundo Frankenfeld et al. (2014) as doses utilizadas sao de 10 a 100 vezes maiores que a dose terapeutica, e este abuso pode causar varios danos ao organismo. De acordo com BOFF (2002), os principais efeitos colaterais dos EAA’s em geral sobre o sistema reprodutor sao nos homens a reducao da producao de espermatozoides, atrofia dos testiculos, impotencia, dificuldade ou dor para urinar, ginecomastia, priapismo, hipertrofia prostatica e carcinoma prostatico. Em mulheres observa-se a virilizacao, manifestando-se com diminuicao da gordura corporal e tamanho dos seios, voz mais grave, irregularidades menstruais, aumento do clitoris e alteracao na libido. No entanto, o uso de DN apresenta efeitos sobre todo o organismo, acarretando serios danos aos diferentes sistemas fisiologicos.
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... e Fisiologia da Efoa/Ceufe, Fernando Ponciano, ao Técnico do Laboratório de Plantas Medicinais e Fitoterápicos da Efoa/Ceufe, Luiz Marcelo da Silva e à Profª Titular aposentada de Fisiologia, Drª Ruth Gazola de Freitas ... Brandão... more
... e Fisiologia da Efoa/Ceufe, Fernando Ponciano, ao Técnico do Laboratório de Plantas Medicinais e Fitoterápicos da Efoa/Ceufe, Luiz Marcelo da Silva e à Profª Titular aposentada de Fisiologia, Drª Ruth Gazola de Freitas ... Brandão MGL, Freire N, Vianna-Soares CD 1998. ...
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A infecção do trato urinário (ITU) é a principal causa de infecções em instituições de saúde, tornando-se resistentes a vários antimicrobianos, devido principalmente ao uso irracional e inadequado destes. Para um melhor tratamento tem... more
A infecção do trato urinário (ITU) é a principal causa de infecções em instituições de saúde, tornando-se resistentes a vários antimicrobianos, devido principalmente ao uso irracional e inadequado destes. Para um melhor tratamento tem sido proposto a implantação de programas de vigilância de resistência antimicrobiana, analisando os padrões locais de susceptibilidade o que auxilia na eleição inicial do antimicrobiano, tornando o tratamento menos oneroso devido a não utilização de fármacos sabidamente ineficazes. O presente estudo objetiva traçar um perfil bacteriológico da incidência de ITUs e da resistência e sensibilidade aos antimicrobianos. A presente pesquisa trata-se de uma estudo descritivo, retrospectivo e transversal, por meio de análise documental de 340 urinoculturas e de seus antibiogramas realizados no período de um ano em uma Instituição Hospitalar. Utilizou-se como tratamento estatístico o teste de Qui-Quadrado de Pearson e “t” de Student-Newman-Keuls (p
Research Interests: Engineering and Holos
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... Segundo Gálvez ... Auxiliar Técnico do Laboratório de Farmacologia e Fisiologia da UNI-FAL-MG, Fernando Ponciano, e ao Técnico do Labo-ratório de Plantas Medicinais e Fitoterápicos da UNI-FAL-MG, Luiz Marcelo da Silva. ... Gonzalez,... more
... Segundo Gálvez ... Auxiliar Técnico do Laboratório de Farmacologia e Fisiologia da UNI-FAL-MG, Fernando Ponciano, e ao Técnico do Labo-ratório de Plantas Medicinais e Fitoterápicos da UNI-FAL-MG, Luiz Marcelo da Silva. ... Gonzalez, & T. Tolon (1989) J. Ethnopharma-col. ...
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Overwhelming evidence supports the importance of the sympathetic nervous system in heart failure. In contrast, much less is known about the role of failing cholinergic neurotransmission in cardiac disease. By using a unique genetically... more
Overwhelming evidence supports the importance of the sympathetic nervous system in heart failure. In contrast, much less is known about the role of failing cholinergic neurotransmission in cardiac disease. By using a unique genetically modified mouse line with reduced expression of the vesicular acetylcholine transporter (VAChT) and consequently decreased release of acetylcholine, we investigated the consequences of altered cholinergic tone for cardiac function. M-mode echocardiography, hemodynamic experiments, analysis of isolated perfused hearts, and measurements of cardiomyocyte contraction indicated that VAChT mutant mice have decreased left ventricle function associated with altered calcium handling. Gene expression was analyzed by quantitative reverse transcriptase PCR and Western blotting, and the results indicated that VAChT mutant mice have profound cardiac remodeling and reactivation of the fetal gene program. This phenotype was attributable to reduced cholinergic tone, si...
Research Interests: Cellular Biology, Calcium, Patch-clamp and imaging techniques, Echocardiography, Biological Sciences, and 14 moreHeart Failure, Molecular and cellular biology, Humans, Hemodynamics, Mutation, Mice, G protein-coupled receptors, Animals, Heart rate, Synaptic Transmission, Phenotype, Sympathetic Nervous System, Ventricular Remodeling, and Cholinergic agents
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Loxosceles spider bites cause many human injuries worldwide. Injections in mice of whole Loxosceles (L.) intermedia venom or a recombinant toxin (rLiD1) produce systemic symptoms similar to those detected in envenomed humans. This animal... more
Loxosceles spider bites cause many human injuries worldwide. Injections in mice of whole Loxosceles (L.) intermedia venom or a recombinant toxin (rLiD1) produce systemic symptoms similar to those detected in envenomed humans. This animal model was used to characterize the effects of Loxosceles intermedia venom in cardiac tissues. L. intermedia antigens were detected by ELISA in kidney, heart, lung and liver of experimentally envenomed mice. In addition, rLiD1 binding to cardiomyocytes was demonstrated by immunofluorescence and confocal microscopy. Furthermore, isolated perfused heart preparations and ventricular cardiomyocytes from envenomed mice showed heart function impairment, and a significant increase of I(Ca,L) density and intracellular Ca(2+) transients, respectively. Thus, L. intermedia spider venom, as shown through the use of the recombinant toxin rLiD1, causes cardiotoxic effects and a protein from the sphingomyelinase D family plays a key role in heart dysfunction. Thus, L. intermedia spider venom and the Loxtox rLiD1 play a key role in heart dysfunction.
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In this study, we evaluated the effects of PhKv, a 4584 Da peptide isolated from the spider Phoneutria nigriventer venom, in the isolated rat heart and in isolated ventricular myocytes. Ventricular arrhythmias were induced by occlusion of... more
In this study, we evaluated the effects of PhKv, a 4584 Da peptide isolated from the spider Phoneutria nigriventer venom, in the isolated rat heart and in isolated ventricular myocytes. Ventricular arrhythmias were induced by occlusion of the left anterior descending coronary artery for 15 min followed by 30 min of reperfusion. Administration of native PhKv (240 nM) 1 min before or after reperfusion markedly reduced the duration of arrhythmias. This effect was blocked by atropine, thereby indicating the participation of muscarinic receptors in the antiarrhythmogenic effect of PhKv. Notably, recombinant PhKv (240 nM) was also efficient to attenuate the arrhythmias (3.8 ± 0.9 vs. 8.0 ± 1.2 arbitrary units in control group). Furthermore, PhKv induced a significant reduction in heart rate. This bradycardia was partially blunted by atropine and potentiated by pyridostigmine. To further evaluate the participation of acetylcholine on the PhKv effects, we examined the release of this neurotransmitter from neuromuscular junctions. It was found that Phkv (200 nM) significantly increased the release of acetylcholine in this preparation. Moreover, PhKv (250 nM) did not cause any significant change in action potential or Ca(2+) transient parameters in isolated cardiomyocytes. Altogether, these findings show an important acetylcholine-mediated antiarrhythmogenic effect of the spider PhKv toxin in isolated hearts.
Research Interests: Electrophysiology, Spiders, Animals, Male, Heart rate, and 18 moreHeart, Calcium Signaling, Acetylcholine, Neurotoxins, Molecular cloning, Rats, Wistar Rats, Action potential, Arrhythmia, Coronary Artery Disease, Action Potentials, Neuromuscular Junction, Muscarinic Receptor, Atropine, Toxicon, Ventricular Arrhythmia, Control Group, and Coronary artery
Cardiac dysfunction is one of the possible causes of sudden unexpected death in epilepsy (SUDEP). Therefore, it was the objective of this study to evaluate the cardiac and electrocardiographic parameters after seizures induced by maximal... more
Cardiac dysfunction is one of the possible causes of sudden unexpected death in epilepsy (SUDEP). Therefore, it was the objective of this study to evaluate the cardiac and electrocardiographic parameters after seizures induced by maximal electroshock (MES) in Wistar rats. Electroshock seizures were induced in Wistar rats through a pair of ear-clip electrodes (10 mA at a frequency of 60 Hz applied for one second). In vivo electrocardiography (ECG) was performed in awake animals for analysis of heart rate variability (HRV) and cardiac rhythm. Ex vivo the Langendorff technique was used to analyze cardiac function and observe the incidence and severity of reperfusion arrhythmias. Convulsive seizures triggered by MES induced profound abnormalities in cardiac rhythm with serious electrocardiographic changes including ST-elevation, bundle branch block, atrioventricular nodal escape rhythm and premature ventricular contractions. ECG analysis demonstrated a consistent period of postictal bradyarrhythmia resulting in a transiently irregular cardiac rhythm with highly variable and prolonged QRS complexes and RR, PR, QT and QTc intervals. HRV evaluation revealed an increase in the high-frequency range of the power, suggesting an imbalance in the autonomic control of the heart with a postictal enhancement of parasympathetic tone. In addition, we observed in isolated heart a decrease in systolic tone and an increase in the coronary flow, heart rate and incidence/duration of ischemia-reperfusion arrhythmias. The present study supports a relationship betweem seizures, cardiac dysfunction and cardiac arrhythmias. This relationship may partially account for the occurrence of SUDEP.