Research Interests:
Research Interests:
Research Interests:
Research Interests:
Purpose: To evaluate the influence of dentin moisture on bond strengths of an etch-and-rinse bonding agent to primary dentin clinically and in the laboratory. Methods: The sample consisted of two groups of 20 caries-free primary second... more
Purpose: To evaluate the influence of dentin moisture on bond strengths of an etch-and-rinse bonding agent to primary dentin clinically and in the laboratory. Methods: The sample consisted of two groups of 20 caries-free primary second molars: molars in exfoliation period (clinical group) and extracted molars (laboratory group). Class I cavities were prepared in all specimens leaving a flat dentin surface on the pulpal floor. A two-step etch-and-rinse adhesive was vigorously rubbed on either dry (n = 5) or wet demineralized dentin (n = 5) under clinical or laboratory conditions. After restorative procedures, the teeth from the clinical group were extracted after 20 minutes. All samples were processed and underwent microtensile bond strength test and silver nitrate uptake evaluation under scanning electron microscopy. Results: Statistically higher bond strength values were observed when the bonding was performed under laboratory conditions and on a wet demineralized dentin. Most of the failures were adhesive and mixed irrespective of the experimental condition. Silver nitrate uptake occurred in all groups irrespective of the experimental condition. Resin-dentin bond strengths produced in the laboratory in primary teeth may overestimate those produced under clinical circumstances.
Research Interests: Materials Science, Dentistry, Scanning Electron Microscopy, Dental Materials, Medicine, and 15 moreAdhesive, Humans, Child, Pubmed, Temperature, Desiccation, Bond Strength, Mechanical Stress, Composite resins, Dentin, Dental Bonding, Materials Testing, Molar, Dentin bonding agents, and Dental leakage
Purpose: This study's purpose was to investigate the effect of a glass ionomer cement as a liner over infected unexcavated dentin after 60 days and at 10 to 15 months. Methods: Forty-five permanent molars with deep carious lesions were... more
Purpose: This study's purpose was to investigate the effect of a glass ionomer cement as a liner over infected unexcavated dentin after 60 days and at 10 to 15 months. Methods: Forty-five permanent molars with deep carious lesions were selected. Fragments of carious dentin were removed prior to lining the cavity (baseline sample) with glass ionomer cement (G1) or an inert wax material (G2). Cavities were restored with composite resin and reopened 60 days later, when other fragments were removed (60-day sample). The dentin morphology (scanning electron microscopy) and mineral content of calcium, phosphorus, and fluorine were assessed. During the follow-up periods (60 days and 10 to 15 months), restorations were evaluated and standardized radiographs were taken. A postprocessing routine was used to identify changes in the radiographic density between periods. Results: After 60 days, the dentin exhibited a better organization, fewer bacteria, and signs of remineralization. The weight percents of calcium and phosphorus were higher 60 days after the cavity sealing, regardless of the group. Higher gray levels of carious and sound dentin were seen on the 10- to 15-month radiographs, irrespective of the group. The success rates of G1 and G2 were 89 percent and 88 percent, respectively. Conclusions: The lining material isn't fundamental for caries arrestment. Early (60-day) and late (10 to 15 months) dentin changes occurred, indicating the remineralization of dentin carious tissue.
Research Interests:
Purpose: To evaluate the efficacy of a new light-cured anesthetic gel for pain control associated with the clamp placement for rubber dam isolation in children. Methods: 82 children in need of sealant placement on the first permanent... more
Purpose: To evaluate the efficacy of a new light-cured anesthetic gel for pain control associated with the clamp placement for rubber dam isolation in children. Methods: 82 children in need of sealant placement on the first permanent mandibular molars were selected to take part in this randomized, split-mouth, triple-blinded study. Before rubber dam placement, the light-cured anesthetic gel (test) or a placebo gel (control) was applied on the gingival margins of the molars. The hemi-arches were isolated with cotton rolls and the gels were applied; the anesthetic gel was light-cured. After 30 seconds, clamp #26 was positioned on tooth 36 or 46. If there was pain, the clamp was removed and rescue anesthesia was applied. The absolute risk and intensity of pain were registered using three scales: facial expression (Wong-Baker), observational (FLACC) and numerical (NRS). Data were analyzed by McNemar's test and Wilcoxon Signed Rank (α= 5%). Results: Differences were detected for the risk of pain between groups (P= 0.0002) and for the different intensity of pain scales used (P< 0.001) with positive results for the anesthetic gel. Clinical significance: The new developed light-cured topical anesthetic is an alternative to infiltrative anesthesia for pain control during clamp adaptation for rubber dam isolation. Its use helps to overcome the fear of needles, which can trigger pain, anxiety and discomfort for the majority of the patients, hampering the behavior management in children.
Research Interests:
Purpose: Discomfort during rubber dam clamp often occurs in pediatric dentistry. The purpose of this randomized, triple-blind, clinical trial was to evaluate the effect of pain reduction using a new topical liposomal thermo-sensitive gel... more
Purpose: Discomfort during rubber dam clamp often occurs in pediatric dentistry. The purpose of this randomized, triple-blind, clinical trial was to evaluate the effect of pain reduction using a new topical liposomal thermo-sensitive gel (TLTG) or a placebo gel during rubber dam clamp isolation for sealants in children. Methods: Eighty-one children (eight to 12 years old) had LTSG or the placebo gel with random assignment placed around the gingival tissue of their permanent mandibular first molars. A clamp was placed after two minutes on the teeth, and intensity of pain was registered using a Wong-Baker FACES scale and an 11-point numerical scale. The data were evaluated using McNemar's test and Wilcoxon signed rank test. Results: The odds ratio for pain (OR equals 0.7; 95 percent confidence interval equals 0.3 to 1.8) was not statistically significant (P=0.52). However, there was a small difference in the pain intensity between liposomal and placebo groups for both scales (P<0.05). Conclusion: The liposomal thermo-sensitive anesthetic gel may reduce, to a small extent, the pain intensity in children submitted to rubber dam isolation before resin sealant placement.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Objective: The aim of this study was to investigate the role of the lining material on dentin changes following restoration without complete removal of infected dentin. Method: Forty-five permanent molars with deep caries lesions were... more
Objective: The aim of this study was to investigate the role of the lining material on dentin changes following restoration without complete removal of infected dentin. Method: Forty-five permanent molars with deep caries lesions were selected from 35 children. Fragments of carious dentin were removed prior to lining the cavity (baseline sample) with high viscosity glass ionomer cement (G1) or an inert material (wax - G2). Cavities were restored with composite resin, re-opened 60 days later and other fragments removed (60-day sample). The dentin morphology and mineral content (calcium, phosphorus, fluorine) of both samples were assessed under SEM. At the follow-up periods (60 days; 10-15 months), restorations were evaluated and standardized radiographs taken. A post-processing routine was used to identify changes in radiographic density between periods. Result: After 60 days, the dentin exhibited a better tissue organization, fewer bacteria and signs of remineralization. The wt% of ...
Objective: This study evaluated the effect of a thermosensitive liposomal anesthetic gel on pain and anxiety during nonsurgical periodontal treatment in patients with special needs. Method: This study was designed as a split mouth... more
Objective: This study evaluated the effect of a thermosensitive liposomal anesthetic gel on pain and anxiety during nonsurgical periodontal treatment in patients with special needs. Method: This study was designed as a split mouth randomized double-bind clinical trial. Thirty-two young subjects (17.4 ± 5.6) of both genders were selected according to the inclusion criteria: moderate intellectual disability, minimum of 16 teeth, at least one tooth in each sextant with probing depth ≥ 4 mm. Prior periodontal treatment, the index of plaque, marginal bleeding, probing pocket depth and bleeding were obtained. The experimental gel tested was composed of 5% prilocaine/lidocaine, microemulsion thermosensitive liposomal phospholipid gel. It was compared to a commercially available gel Oraqix® (5% prilocaine/lidocaine-thermosensitive gel).The negative controls for both gels were periodontal treatment without anesthesia and the rescue drug used was EMLA® cream (5% prilocaine/lidocaine), applied...
Objective: this study main objective was to evaluate a non invasive, clinic diagnostic tool (DIAGNOdent) to analyse carious deciduous dentin and relate the obtained data to in vitro microhardness values. Method: Ten human deciduous molar... more
Objective: this study main objective was to evaluate a non invasive, clinic diagnostic tool (DIAGNOdent) to analyse carious deciduous dentin and relate the obtained data to in vitro microhardness values. Method: Ten human deciduous molar teeth with acute caries lesions were selected by a trained examiner. Caries depth was confirmed by radiographs. All teeth were cleaned and longitudinally sectioned in order to obtain at least two slices of approximately 1mm thickness, containing health and carious dentin. After polishing, dentin fluorescence (DIAGNOdent - Kavo) and Knoop microhardness were measured. The values considered for statistical analysis were the arithmetical means obtained after three repeated measures on each slice for health and carious dentin. The testes used were Mann-Whitney and Pearson correlation (α=0.05). Result: It was observed that carious dentin showed lower values of hardness when compared to sound dentin (p<0,0001); but its fluorescence was higher (p<0,00...
Objective: to compare qualitative criteria with laser fluorescence in evaluating deciduous carious dentin after cavity sealing. Method: Forty three vital primary molars with acute caries lesions were selected from children aging 3 to 9... more
Objective: to compare qualitative criteria with laser fluorescence in evaluating deciduous carious dentin after cavity sealing. Method: Forty three vital primary molars with acute caries lesions were selected from children aging 3 to 9 years old. After removing all carious dentin from dentin-enamel junction and lateral cavity walls, the necrotic dentin from cavity floor was removed roughly. At this moment (baseline - day 0), the first fluorescence reading was made using DIAGNOdent device (Kavo, Biberach, Germany). Teeth were restored with a high viscosity glass ionomer cement (Ketac Molar Easy Mix - 3M ESPE) and reopened after 60 days to obtain a new fluorescence reading (experimental period - day 60). Qualitative criteria were also evaluated on days 0 and 60 by a calibrated examiner (k=0.89 and 0.93 for dentin color and consistence, respectively). After checking data normality (Kolmogorov-Smirnov test), statistical analysis was performed using Wilcoxon test for paired samples (α=0....
Dentifrices with different compositions are available on the market, but there is limited information about their properties. The aim of this in vitro study was to evaluate the physicochemical characteristics of 12 dentifrices divided... more
Dentifrices with different compositions are available on the market, but there is limited information about their properties. The aim of this in vitro study was to evaluate the physicochemical characteristics of 12 dentifrices divided into three categories, as containing desensitizing agents, Triclosan or whitening agents. Desiccation loss/residue analysis: 5 g of dentifrice was weighed five times for each group. pH analysis: 5 g of dentifrice were diluted in three parts of distilled water and analyzed using a digital potentiometer. Scanning electron microscopy (SEM): analysis of ashes, shape and size of the particles. Energy-dispersive x-ray spectroscopy (EDX): identification of the abrasive elements. Data were analyzed using ANOVA, Bonferroni post hoc test (α=0.05). Desiccation loss: 38.21% to 65.83%. Dentifrices containing Triclosan and desensitizing agents showed statistically significant differences among them (p<0.05). Whitening dentifrices showed statistically significant ...
Research Interests:
Research Interests:
ABSTRACT Objectives: To evaluate the influence of dentin moisture on the degradation of the resin-dentin bond strengths to deciduous dentin under clinical and laboratory conditions. Materials and methods: Class I cavities were prepared in... more
ABSTRACT Objectives: To evaluate the influence of dentin moisture on the degradation of the resin-dentin bond strengths to deciduous dentin under clinical and laboratory conditions. Materials and methods: Class I cavities were prepared in specimens leaving a flat dentine surface on the pulpal floor. A two-step etch-and-rinse adhesive was vigorously rubbed on either dry (n=5) or wet demineralised dentin (n=5) under in vitro or in vivo conditions. After restorative procedures, teeth from in vivo group were extracted after 20 min or exfoliated after 6 months. Teeth from in vitro groups were stored in water for 20 min or 6 months. In the respective testing periods, specimens were prepared for microtensile testing (mTBS). Representative specimens were also evaluated for silver nitrate uptake (SNU) under scanning electron microscopy. The mTBS (MPa) and SNU (%) data were submitted to a 3-way ANOVA and Tukey&#39;s test. Results: Statistically higher mTBS values were observed when the bonding was performed under laboratory conditions (p&lt;0.05). Degradation of the mTBS occurred only in the wet dentin groups in both experiments (p&lt;0.05). SNU occurred in all groups and was statistically higher after aging (p&lt;0.05). Microtensile bond strength values for the experimental groups (MPa). Testing period In vivo In vitro Dry Wet Dry Wet Immediate 24.13.5 28.72.1 29.23.1 33.43.0 6 months 25.81.6 21.11.8 28.93.0 21.62.3 Conclusions: Resin-dentin bond strengths produced in deciduous in laboratory conditions teeth may overestimate those obtained in clinical circumstances although both conditions (clinical and laboratory) seemed to yield similar conclusions. Bonding to dry demineralized deciduous dentin produced resin-dentin interfaces more resistant to degradation than those formed in wet demineralized dentin. .
Background: Early childhood caries (ECC) has a profound impact on a child's quality of life, and its management remains a challenge for the paediatric dentist, mainly because it depends on radical changes in the child/carers' daily... more
Background: Early childhood caries (ECC) has a profound impact on a child's quality of life, and its management remains a challenge for the paediatric dentist, mainly because it depends on radical changes in the child/carers' daily behaviour and any dental treatment must be provided to very young child. Case report: This case report describes the on-going care of a 2-year-old child presenting with ECC and management until permanent dentition is complete. All patient-focused, teamdelivered care was delivered using the minimum intervention oral care framework, implementing non-invasive and minimally invasive preventive procedures. Throughout the care provided, oral and dental health education was reinforced in all visits. The child's mother was trained to perform effective biofilm control and dietary habits were adjusted, especially baby-bottle removal during sleep. The child was initially anxious and resistant towards any dental examination and clinical procedures. However, with effort from the oral healthcare team members, the patient became compliant, allowing the mother to perform suitable oral hygiene measures, as well as accepting the clinical procedures carried out by the paediatric dentist. The clinical procedures consisted of atraumatic restorations and fluoride varnish applications. During the subsequent years after the baseline treatment, follow-up visits included continued dietary and oral hygiene instruction with positive behavior reinforcement, fluoride topical applications and tooth-restoration complex maintenance with glass-ionomer cement where needed. Currently, the patient is 19 years old and has a stable, healthy permanent dentition. Conclusion: Understanding of the causes of oral diseases by the patients' caregivers, alongside with pragmatic practical guidance to maintain good oral health, can reduce the risk for acquiring future disease, since caries activity control is the basis for successful caries management.
Research Interests:
Research Interests: Humanities and Medicine
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
A systematic review/meta-analysis was performed to evaluate pain during probing, scaling and root planing using intra-pocket anaesthesia versus placebo in adult patients. A search was performed in PubMed, Scopus, Web of Science, Latin... more
A systematic review/meta-analysis was performed to evaluate pain during probing, scaling and root planing using intra-pocket anaesthesia versus placebo in adult patients. A search was performed in PubMed, Scopus, Web of Science, Latin American and Caribbean Health Sciences Literature database, Brazilian Library in Dentistry, Cochrane Library and Grey literature. IADR abstracts, unpublished trials registries, dissertations and theses were also searched for randomized clinical trials comparing the clinical effectiveness of intra-pocket anaesthesia and placebo. Risk/intensity of pain was the primary outcome. The risk of bias tool from the Cochrane Collaboration was used for quality assessment. Meta-analysis was performed on studies considered at low risk of bias. A total of 1740 articles were identified. Eleven remained in the qualitative synthesis, and nine studies were considered at &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;low&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; risk of bias for meta-analysis. Standardized Hedge&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s g mean difference for pain intensity using visual analogue scale and Heft-Parker pain scales was -0.576 (95% confidence interval [CI] -0.94 to -0.22; p = 0.002) and for verbal rating scale pain scale it was -1.814 (95% CI -3.38 to -0.245; p = 0.023). The odds ratio for the risk of pain was 0.025 (95% CI 0.003 to 0.25; p = 0.002) and the odds ratio for the need for rescue anaesthesia it was 0.358 (95% CI 0.174 to 0.736; p = 0.005). The anaesthetic gel decreases the risk and intensity of pain during probing/SRP.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Introduction: Partial caries removal has been shown to be an effective method to treat deep carious lesions in deciduous teeth. Nevertheless, the possibility of keeping infected dentin in the cavity still requires additional... more
Introduction: Partial caries removal has been shown to be an effective method to treat deep carious lesions in deciduous teeth. Nevertheless, the possibility of keeping infected dentin in the cavity still requires additional investigation. The objective of this research was to describe changes in primary infected dentin after restoration with glass ionomer cement. Methods: Dentin from 45 primary molars with deep and active carious lesions was evaluated using clinical and laboratory criteria, before and 60 days after restoration. The clinical analysis evaluated dentin colour (CO), dentin consistency (COS) and laser fluorescence (LF). The laboratory procedures assessed bacterial contamination and mineral content (MC), and evaluated the dentin ultrastructure and collagen content. Data on CO, COS, LF and colony forming units were analysed using the Wilcoxon signed-rank test; MC, bacterial counts and collagen evaluations were evaluated using the Student’s t-test. Results: After 60 days, lower values of LF were observed, together with a lower bacterial count, and a higher COS was found, with an increase in calcium, phosphorus and collagen contents. Differences were not detected for CO or for fluorine content. Baseline samples showed enlarged tubules with bacterial invasion; 60-day samples showed better organised tissue, with a more compact intertubular dentin and narrower tubules. Conclusion: It is concluded that appropriate cavity sealing can promote beneficial changes in deep carious lesions of primary teeth, even in the presence of infected dentin.
Research Interests:
Research Interests:
The authors conducted a study to evaluate the influence of dentin moisture on the degradation of the resin-dentin interface in primary teeth under clinical and laboratory conditions. The authors prepared 40 Class I restorations (five... more
The authors conducted a study to evaluate the influence of dentin moisture on the degradation of the resin-dentin interface in primary teeth under clinical and laboratory conditions. The authors prepared 40 Class I restorations (five teeth per group) by using a cylindrical diamond bur, leaving a flat dentin surface on the pulpal floor. They vigorously rubbed two coats of a simplified etch-and-rinse adhesive on either dry or wet demineralized dentin under clinical or laboratory conditions. After performing restorative procedures, the authors extracted teeth prepared under clinical conditions after 20 minutes (immediately) or the teeth exfoliated after six months. The authors also tested the teeth prepared under laboratory conditions immediately or after six months of being stored in water. They sectioned the teeth to obtain resin-dentin bonded specimens for microtensile testing and for silver nitrate uptake (SNU) under scanning electron microscopy. They performed a three-way analysis of variance and Tukey test (α = .05) on the SNU bond strength data. Statistically higher bond strength values (megapascals [standard deviation]) were observed when bonding was performed under laboratory conditions (clinical = 25.2 [3.6] MPa versus laboratory = 28.5 [4.4] MPa; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; .05). Degradation occurred only in the wet dentin groups under both experimental conditions (immediately = 31.3 [4.5] MPa versus after six months = 21.3 [2.1] MPa; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; .05). SNU occurred in all groups and was statistically higher after six months of clinical function or water storage (immediately = 13.9 [4.9 SD] percent versus after six months = 34.1 [4.5 SD] percent; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; .05). The bonding of adhesives to dry demineralized dentin produces adhesive interfaces that are more resistant to degradation regardless of the bonding condition. Resin-dentin bond strengths produced under laboratory conditions in primary teeth may be higher than those obtained under clinical circumstances, although both conditions (clinical and laboratory) seemed to yield similar results. Bonding to dry demineralized primary tooth dentin produced resin-dentin interfaces that were more resistant to degradation.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
The purpose of this study was to compare the survival rate of ART (atraumatic restorative treatment) Class II restorations in primary teeth, performed with glass carbomer cement and a high-viscosity glass ionomer cement, after 12 months.... more
The purpose of this study was to compare the survival rate of ART (atraumatic restorative treatment) Class II restorations in primary teeth, performed with glass carbomer cement and a high-viscosity glass ionomer cement, after 12 months. One pediatric dentist placed 59 Class II ART restorations in 33 children (eight plus/minus two years old) of both genders. Two calibrated examiners, blinded to the type of material and not involved in the placement, evaluated the restorations at baseline, six, and 12 months. The overall success rate (95 percent confidence interval) at the six-month follow-up for the GP Glass Fill and Equia Fil was 69 percent (51 to 83) and 83 percent (66 to 93), respectively. No significant difference was detected between the study groups (Fisher's exact test, P=0.20). However, at 12 months, the overall success rates of both materials were 56 percent (37 to 73) and 86 percent (69 to 94), and this difference was statistically significant (Fisher's exact test,...
Research Interests: Dentistry, Survival Analysis, Scanning Electron Microscopy, Medicine, Humans, and 13 moreChild, Female, Pubmed, Dental Caries, Male, Follow-up studies, Pediatric dentistry, Pediatric Dentistry, Apatites, Confidence Interval, Glass Ionomer Cement, Paediatrics and reproductive medicine, and Glass ionomer cements
A systematic review/meta-analysis was performed to evaluate pain during probing, scaling and root planing using intra-pocket anaesthesia versus placebo in adult patients. A search was performed in PubMed, Scopus, Web of Science, Latin... more
A systematic review/meta-analysis was performed to evaluate pain during probing, scaling and root planing using intra-pocket anaesthesia versus placebo in adult patients. A search was performed in PubMed, Scopus, Web of Science, Latin American and Caribbean Health Sciences Literature database, Brazilian Library in Dentistry, Cochrane Library and Grey literature. IADR abstracts, unpublished trials registries, dissertations and theses were also searched for randomized clinical trials comparing the clinical effectiveness of intra-pocket anaesthesia and placebo. Risk/intensity of pain was the primary outcome. The risk of bias tool from the Cochrane Collaboration was used for quality assessment. Meta-analysis was performed on studies considered at low risk of bias. A total of 1740 articles were identified. Eleven remained in the qualitative synthesis, and nine studies were considered at "low" risk of bias for meta-analysis. Standardized Hedge's g mean difference for pain in...
Research Interests:
Research Interests:
Research Interests:
This study's purpose was to describe the primary dentin reactions following restoration without complete removal of infected dentin. Fragments of carious dentin from 43 teeth with acute deep lesions were removed at baseline and... more
This study's purpose was to describe the primary dentin reactions following restoration without complete removal of infected dentin. Fragments of carious dentin from 43 teeth with acute deep lesions were removed at baseline and compared to samples of carious dentin removed 60 days later, using scanning electron microscopy (SEM) photographs. At the follow-up periods (60 days; 10-13 months), restorations were evaluated using US Public Health Service criteria and standardized radiographs. A postprocessing routine of radiographs was used to identify changes in radiographic density between periods. At baseline, SEM photographs showed disorganized tissue and bacterial invasion. After 60 days, the dentin exhibited a better organization and signs of remineralization. Differences in restoration characteristics were detected for cavosurface discoloration (P=.008), wear (P<.001), and surface texture (P<.001) after 10 to 13 months. Digital subtraction radiographs showed that mean gray...
Research Interests:
To cite this article:
Int J Dent Hygiene 9, 2011; 149–154
DOI: 10.1111/j.1601‐5037.2010.00476.x.
Stroski ML, de Souza Dal Maso AM, Wambier LM, Chibinski ACR, Pochapski MT, Santos FA, Wambier DS. Clinical evaluation of three toothbrush... more
To cite this article:
Int J Dent Hygiene 9, 2011; 149–154
DOI: 10.1111/j.1601‐5037.2010.00476.x.
Stroski ML, de Souza Dal Maso AM, Wambier LM, Chibinski ACR, Pochapski MT, Santos FA, Wambier DS. Clinical evaluation of three toothbrush models tested by schoolchildren.: Aim: The aim of this single‐blind cross‐over study was to compare the performance of three different toothbrush models in the control of dental biofilm and maintaining a healthy gingival condition.Methods: Twenty‐seven schoolchildren (aged 9–10 years) participated in the study. Three toothbrushes with different bristle arrangements were used: T1 – bristles on the same plane, straight arrangement; T2 – bristles on different planes, straight arrangement; T3 – bristles on different planes, straight and circular arrangement. The participants were then randomly divided into three groups for brushing with one of the three toothbrushes. Each experimental period lasted 15 days each, with three daily brushings and a 7‐day ...
Research Interests:
The authors conducted a study to evaluate the influence of dentin moisture on the degradation of the resin-dentin interface in primary teeth under clinical and laboratory conditions. The authors prepared 40 Class I restorations (five... more
The authors conducted a study to evaluate the influence of dentin moisture on the degradation of the resin-dentin interface in primary teeth under clinical and laboratory conditions. The authors prepared 40 Class I restorations (five teeth per group) by using a cylindrical diamond bur, leaving a flat dentin surface on the pulpal floor. They vigorously rubbed two coats of a simplified etch-and-rinse adhesive on either dry or wet demineralized dentin under clinical or laboratory conditions. After performing restorative procedures, the authors extracted teeth prepared under clinical conditions after 20 minutes (immediately) or the teeth exfoliated after six months. The authors also tested the teeth prepared under laboratory conditions immediately or after six months of being stored in water. They sectioned the teeth to obtain resin-dentin bonded specimens for microtensile testing and for silver nitrate uptake (SNU) under scanning electron microscopy. They performed a three-way analysis of variance and Tukey test (α = .05) on the SNU bond strength data. Statistically higher bond strength values (megapascals [standard deviation]) were observed when bonding was performed under laboratory conditions (clinical = 25.2 [3.6] MPa versus laboratory = 28.5 [4.4] MPa; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; .05). Degradation occurred only in the wet dentin groups under both experimental conditions (immediately = 31.3 [4.5] MPa versus after six months = 21.3 [2.1] MPa; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; .05). SNU occurred in all groups and was statistically higher after six months of clinical function or water storage (immediately = 13.9 [4.9 SD] percent versus after six months = 34.1 [4.5 SD] percent; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; .05). The bonding of adhesives to dry demineralized dentin produces adhesive interfaces that are more resistant to degradation regardless of the bonding condition. Resin-dentin bond strengths produced under laboratory conditions in primary teeth may be higher than those obtained under clinical circumstances, although both conditions (clinical and laboratory) seemed to yield similar results. Bonding to dry demineralized primary tooth dentin produced resin-dentin interfaces that were more resistant to degradation.
Research Interests:
The objective of this in vivo study was to compare the expression of matrix metalloproteinases (MMP-2, MMP-8, MMP-9), type I collagen and bone sialoprotein (BSP) in infected dentin of primary teeth at baseline and after cavity sealing... more
The objective of this in vivo study was to compare the expression of matrix metalloproteinases (MMP-2, MMP-8, MMP-9), type I collagen and bone sialoprotein (BSP) in infected dentin of primary teeth at baseline and after cavity sealing with glass ionomer cement. Dentin samples from 45 primary molars with deep and active carious lesions were collected before (baseline sample) and after cavity sealing (60-day sample). The samples were fixed, demineralized and processed for immunohistochemistry assays. Monoclonal antibodies were used for the localization of the cited antigens with an avidin-biotin method. Digital images of the sections were captured and analyzed with ImageJ software. The mean intensity of RGB channels in the images was obtained and compared using Student's t test (α = 0.05). The expression of the MMPs, type I collagen and BSP increased after sealing, but statistical differences were observed only for MMP-8, type I collagen and BSP. MMP-2 and MMP-9 were more concentr...