Gabriela Hernandez

Additional file 2: Supplemental Table 1. Top 25 genes classifying the eight distinct astrocyte clusters identified in scRNAseq analysis of E3 and E4 mouse brains (clusters visualized in Fig. 1e). Supplemental Table 2. Age, sex, and APOE genotype of cognitively normal individuals according to Ε4 carriage and age cohort (young = 18–39, middle-aged = 40–65). Values represent means +/− (SD). Supplemental Table 3. Clinical characteristics of cognitively unimpaired individuals according to Ε4 carriage and age cohort (young = 18–39, middle-aged = 40–65). Values represent means +/− (SD). Ca, Caucasian; AA, African American; His, Hispanic; A, Asian; BMI, body mass index. Supplemental Table 4. Plasma metabolites of study participants analyzed by gas chromatography – before and after a dietary glucose challenge. Supplemental Table 5. Pre-screening checklist. A response of "yes" to any of the following resulted in exclusion from the study. Supplemental Fig. 1. Cluster cell counts. Num...

Background: The National Institute on Aging (NIA)/Alzheimer’s Association (AA) 2018 framework conceptualizes Alzheimer’s disease (AD) biologically. Evidence of brain amyloid by biomarkers defines AD pathologic change and the Alzheimer’s continuum. The presence of tau or neurodegeneration in the absence of amyloid defines non-AD pathologic change. Objective: To examine the relation of in vivo amyloid and neurodegeneration with verbal learning, one of the cognitive abilities affected early in AD, in late middle age. Methods: This was a cross-sectional study of amyloid and neurodegeneration biomarkers in a community-based cohort of 350 late-middle aged Hispanics without dementia (mean age: 64.15±3.34; 72.0%women). Amyloid (A) was measured as global standardized uptake value ratio (SUVR) with 18F-Florbetaben positron emission tomography (PET). Neurodegeneration (N) was ascertained as cortical thickness (CT) in AD signature areas using brain magnetic resonance imaging. We examined A/N co...

Predicting protein-ligand binding affinities constitutes a key computational method in the early stages of the drug discovery process. Molecular docking programs attempt to predict them by using mathematical approximations, namely, scoring functions. In the last years, several scoring functions have been developed, encompassing different terms, from electrostatic forces to protein-ligand interaction fingerprints and beyond. However, it has been noticed that usually each individual scoring function cannot be generalized and its predictive power is arguable. The aim of this study is to improve the binding affinity prediction by finding potential models to combine ten different scoring functions, exploiting machine learning techniques.

By Gabriela Hernandez University of the Pacific 2020 The purpose of this study was to investigate the relationship between early eye tracking and later non-suicidal self-injury, and whether this relationship was negatively impacted by distress and impulsivity, and conversely alleviated by emotional regulation and problem solving abilities. Briggs-Gowan et al. (2001) found that young children with recurrent and comorbid externalizing and internalizing problems have the most impairment; they exhibit greater difficulty with emotional stability, and require greater utilization of mental health services. Little research has focused on the relationship between eye tracking in early infancy and suicidal selfinjurious (NSSI) behavior in early childhood. Multiple regression analysis was used to determine if eye-tracking in early infancy and later self-injurious behavior is mediated by impulsivity, distress, problem solving, and emotion regulation; for which child’s gender and income-toneeds ...

Background Cerebral glucose hypometabolism is consistently observed in individuals with Alzheimer’s disease (AD), as well as in young cognitively normal carriers of the Ε4 allele of Apolipoprotein E (APOE), the strongest genetic predictor of late-onset AD. While this clinical feature has been described for over two decades, the mechanism underlying these changes in cerebral glucose metabolism remains a critical knowledge gap in the field. Methods Here, we undertook a multi-omic approach by combining single-cell RNA sequencing (scRNAseq) and stable isotope resolved metabolomics (SIRM) to define a metabolic rewiring across astrocytes, brain tissue, mice, and human subjects expressing APOE4. Results Single-cell analysis of brain tissue from mice expressing human APOE revealed E4-associated decreases in genes related to oxidative phosphorylation, particularly in astrocytes. This shift was confirmed on a metabolic level with isotopic tracing of 13C-glucose in E4 mice and astrocytes, whic...

Cerebral glucose hypometabolism is consistently observed in individuals with Alzheimer’s disease (AD), as well as in young cognitively normal carriers of the E4 allele of Apolipoprotein E (APOE), the strongest genetic predictor of late-onset AD. While this clinical feature has been described for over two decades, the mechanism underlying these changes in cerebral glucose metabolism remains a critical knowledge gap in the field. Here, we undertook a multi-omic approach by combining single-cell RNA sequencing (scRNAseq) and stable isotope resolved metabolomics (SIRM) to define a metabolic rewiring across astrocytes, brain tissue, mice, and human subjects expressing APOE4. Single-cell analysis of brain tissue from mice expressing human APOE revealed E4-associated decreases in genes related to oxidative phosphorylation, particularly in astrocytes. This shift was confirmed on a metabolic level with isotopic tracing of 13C-glucose in E4 mice and astrocytes, which showed decreased pyruvate...

The Rhizobium genome can be considered as a complex structure, formed by the chromosome and high molecular weight plasmids, that contains a large amount of reiterated DNA sequences and presents frequent rearrangements. A general approach to detect reiterated DNA sequences estimated in Rhizobium etli the presence of 700 reiterated elements belonging to 200 different families; high levels of DNA reiteration were also observed in R. meliloti and in Agrobacterium tumefaciens (Flores et al, 1987). This degree of DNA reiteration is unusual among bacteria; only in Archaebacteria and in Streptomyces such large amounts of reiterated DNA sequences have been shown (Hopwood, Kieser, 1990; Krawiec, Riley, 1990). Independent of its specific biological significance, reiterated DNA sequences function as sites for homologous recombination resulting in genomic rearrangements.

Resumen Introducción: Trichinella spiralis es el parásito causante de la Trichinellosis una zoonosis endémica en nuestro estado la cual afecta a mamíferos entre ellos el humano. La principal causa de infección para este es el consumo de carne mal cocida de cerdo ...