Objectives: To estimate the prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency amo... more Objectives: To estimate the prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency among Saudi men, and to establish the frequency of the two mutations/polymorphisms associated with the G6PD A- mutation - G to A at nucleotide 202 (G202A) and A to G at nucleotide 376 (A376G) - in those found to have G6PD deficiency. Methods: Blood samples were obtained from healthy male Saudi donors and screened for G6PD deficiency using a fluorescent spot test. Samples from subjects shown to be G6PD deficient and controls were then analysed for the presence of the G202A and A376G mutations on exons 4 and 5, respectively, of the G6PD gene using polymerase chain reaction followed by restriction fragment length polymorphism. Result: A total of 2100 male subjects were screened; of these, 100 (4.76%) were shown to be G6PD deficient. The G6PD A- mutation (presence of both G202A and A376G) was observed in two (2%) of the 100 subjects with G6PD deficiency. There was no significant difference in t...
The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-si... more The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-sis and secretion under conditions in which stress to the endoplasmic reticu-lum is increased in β-cells. In this case-control study, we have selected rs7612462 polymorphism within UBE2E2 gene to identify in a Saudi population the type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p < 0.05). All of the subjects used in this study were non-obese (25 < BMI < 30). None of the alleles or genotypes of rs7612462 were associated with T2DM (OR=1.251, 95% CI=0.7703-2.034; p=0.3641). Our data suggest that rs7612462 polym...
Journal of environmental biology / Academy of Environmental Biology, India, 2015
Obesity is a complex metabolic disorder linked to an increased risk of the most common and severe... more Obesity is a complex metabolic disorder linked to an increased risk of the most common and severe human diseases. Several flavonoids are known to have lipolytic activity influencing lipolysis and adipogenesis in adipose cells. In the current study, the inhibitory effect of various flavonoid compounds on fat mass and obesity associated protein (FTO) was assessed. The protein structure of FTO (3LFM) was downloaded from Protein Data Bank. The inhibitory effect of flavonoids was compared with a known clinical anti obesity drug. Autodock tools were used for docking flavonoids and antiobesity drug orlistat with FTO. It was examined that flavonoid quercetin proved maximum affinity (most negative AG), while daidzein showed no affinity towards FTO. The empathy of other flavonoids was in the order of Exemestane > Kaempherol > Letrozole > Rutin. It was concluded that flavonoids (particularly quercetin) may act as an effective drug against fat mass and obesity associated protein. Anti ...
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 2010
To investigate the type 2 diabetes mellitus (T2DM) prevalence and related risk factors in adult p... more To investigate the type 2 diabetes mellitus (T2DM) prevalence and related risk factors in adult population with obesity in Tianjin. With stratified cluster randomized sampling, 2888 obese people with BMI ≥ 28 kg/m(2), aged 18 years old and over were selected from three urban and three rural regions of Tianjin, in 2006. Information on risk factors was collected with questionnaire through face-to-face interview by trained workers and data on fasting blood glucose (FBG) was collected at the same time. 2hrPPG was tested among the people who's FBG ≥ 6.1 mmol/L at the hospital. Prevalence of T2DM was calculated and the distribution of T2DM in the described subgroups and the risk factors analyzed with SPSS software. The prevalence of T2DM in adult population with obesity was 11.74%, with females (13.90%) higher than males (8.75%). The prevalence rates of T2DM were statistically different among different groups, classified by age, education, occupation, district and BMI. Results from th...
The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-si... more The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-sis and secretion under conditions in which stress to the endoplasmic reticu-lum is increased in β-cells. In this case-control study, we have selected rs7612462 polymorphism within UBE2E2 gene to identify in a Saudi population the type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p<0.05). All of the subjects used in this study were non-obese (25<BMI<30). None of the alleles or genotypes of rs7612462 were associated with T2DM (OR=1.251, 95% CI=0.7703-2.034; p=0.3641). Our data suggest that rs7612462 polymorphis...
The evolutionary conservation of a housekeeping gene such as G6PD is greater than that of tissue-... more The evolutionary conservation of a housekeeping gene such as G6PD is greater than that of tissue-specific genes, presumably because the latter may require more specific adaptation to the physiology of individual organisms. The abundance of distinct mutation sites and their clinical manifestations make G6PD ideal for structure-function analysis. Therefore, it is of interest to screen of G6PD deficiency in the blood donors in Kingdom of Saudi Arabia. We report the mean and variation of enzyme activity in a huge set of Suadi to non-Saudi population with reference to the entire population. The sequence level conservation of G6PD among distant species is demonstrated using phylogenetic trees. These observations have implications in the sequence-structure-function understanding of G6PD with reference to its association to several human diseases.
Recently, wireless sensor network (WSN) applications have seen an increase in interest. In search... more Recently, wireless sensor network (WSN) applications have seen an increase in interest. In search and rescue, battlefield reconnaissance, and some other such applications, so that a survey of the area of interest can be made collectively, a set of mobile nodes is deployed. Keeping the network nodes connected is vital for WSNs to be effective. The provision of connectivity can be made at the time of startup and can be maintained by carefully coordinating the nodes when they move. However, if a node suddenly fails, the network could be partitioned to cause communication problems. Recently, several methods that use the relocation of nodes for connectivity restoration have been proposed. However, these methods have the tendency to not consider the potential coverage loss in some locations. This paper addresses the concerns of both connectivity and coverage in an integrated way so that this gap can be filled. A novel algorithm for simultaneous-node repositioning is introduced. In this approach, each neighbour of the failed node, one by one, moves in for a certain amount of time to take the place of the failed node, after which it returns to its original location in the network. The effectiveness of this algorithm has been verified by the simulation results.
Objectives: To estimate the prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency amo... more Objectives: To estimate the prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency among Saudi men, and to establish the frequency of the two mutations/polymorphisms associated with the G6PD A- mutation - G to A at nucleotide 202 (G202A) and A to G at nucleotide 376 (A376G) - in those found to have G6PD deficiency. Methods: Blood samples were obtained from healthy male Saudi donors and screened for G6PD deficiency using a fluorescent spot test. Samples from subjects shown to be G6PD deficient and controls were then analysed for the presence of the G202A and A376G mutations on exons 4 and 5, respectively, of the G6PD gene using polymerase chain reaction followed by restriction fragment length polymorphism. Result: A total of 2100 male subjects were screened; of these, 100 (4.76%) were shown to be G6PD deficient. The G6PD A- mutation (presence of both G202A and A376G) was observed in two (2%) of the 100 subjects with G6PD deficiency. There was no significant difference in t...
The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-si... more The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-sis and secretion under conditions in which stress to the endoplasmic reticu-lum is increased in β-cells. In this case-control study, we have selected rs7612462 polymorphism within UBE2E2 gene to identify in a Saudi population the type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p < 0.05). All of the subjects used in this study were non-obese (25 < BMI < 30). None of the alleles or genotypes of rs7612462 were associated with T2DM (OR=1.251, 95% CI=0.7703-2.034; p=0.3641). Our data suggest that rs7612462 polym...
Journal of environmental biology / Academy of Environmental Biology, India, 2015
Obesity is a complex metabolic disorder linked to an increased risk of the most common and severe... more Obesity is a complex metabolic disorder linked to an increased risk of the most common and severe human diseases. Several flavonoids are known to have lipolytic activity influencing lipolysis and adipogenesis in adipose cells. In the current study, the inhibitory effect of various flavonoid compounds on fat mass and obesity associated protein (FTO) was assessed. The protein structure of FTO (3LFM) was downloaded from Protein Data Bank. The inhibitory effect of flavonoids was compared with a known clinical anti obesity drug. Autodock tools were used for docking flavonoids and antiobesity drug orlistat with FTO. It was examined that flavonoid quercetin proved maximum affinity (most negative AG), while daidzein showed no affinity towards FTO. The empathy of other flavonoids was in the order of Exemestane > Kaempherol > Letrozole > Rutin. It was concluded that flavonoids (particularly quercetin) may act as an effective drug against fat mass and obesity associated protein. Anti ...
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 2010
To investigate the type 2 diabetes mellitus (T2DM) prevalence and related risk factors in adult p... more To investigate the type 2 diabetes mellitus (T2DM) prevalence and related risk factors in adult population with obesity in Tianjin. With stratified cluster randomized sampling, 2888 obese people with BMI ≥ 28 kg/m(2), aged 18 years old and over were selected from three urban and three rural regions of Tianjin, in 2006. Information on risk factors was collected with questionnaire through face-to-face interview by trained workers and data on fasting blood glucose (FBG) was collected at the same time. 2hrPPG was tested among the people who's FBG ≥ 6.1 mmol/L at the hospital. Prevalence of T2DM was calculated and the distribution of T2DM in the described subgroups and the risk factors analyzed with SPSS software. The prevalence of T2DM in adult population with obesity was 11.74%, with females (13.90%) higher than males (8.75%). The prevalence rates of T2DM were statistically different among different groups, classified by age, education, occupation, district and BMI. Results from th...
The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-si... more The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-sis and secretion under conditions in which stress to the endoplasmic reticu-lum is increased in β-cells. In this case-control study, we have selected rs7612462 polymorphism within UBE2E2 gene to identify in a Saudi population the type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p<0.05). All of the subjects used in this study were non-obese (25<BMI<30). None of the alleles or genotypes of rs7612462 were associated with T2DM (OR=1.251, 95% CI=0.7703-2.034; p=0.3641). Our data suggest that rs7612462 polymorphis...
The evolutionary conservation of a housekeeping gene such as G6PD is greater than that of tissue-... more The evolutionary conservation of a housekeeping gene such as G6PD is greater than that of tissue-specific genes, presumably because the latter may require more specific adaptation to the physiology of individual organisms. The abundance of distinct mutation sites and their clinical manifestations make G6PD ideal for structure-function analysis. Therefore, it is of interest to screen of G6PD deficiency in the blood donors in Kingdom of Saudi Arabia. We report the mean and variation of enzyme activity in a huge set of Suadi to non-Saudi population with reference to the entire population. The sequence level conservation of G6PD among distant species is demonstrated using phylogenetic trees. These observations have implications in the sequence-structure-function understanding of G6PD with reference to its association to several human diseases.
Recently, wireless sensor network (WSN) applications have seen an increase in interest. In search... more Recently, wireless sensor network (WSN) applications have seen an increase in interest. In search and rescue, battlefield reconnaissance, and some other such applications, so that a survey of the area of interest can be made collectively, a set of mobile nodes is deployed. Keeping the network nodes connected is vital for WSNs to be effective. The provision of connectivity can be made at the time of startup and can be maintained by carefully coordinating the nodes when they move. However, if a node suddenly fails, the network could be partitioned to cause communication problems. Recently, several methods that use the relocation of nodes for connectivity restoration have been proposed. However, these methods have the tendency to not consider the potential coverage loss in some locations. This paper addresses the concerns of both connectivity and coverage in an integrated way so that this gap can be filled. A novel algorithm for simultaneous-node repositioning is introduced. In this approach, each neighbour of the failed node, one by one, moves in for a certain amount of time to take the place of the failed node, after which it returns to its original location in the network. The effectiveness of this algorithm has been verified by the simulation results.
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