Nascer e Crescer, Birth and Growth Medical Journal. Vol. XXVI, Suplement II. S30. , 2017
Infertility affects around 15% of couples worldwide, and from these, males are found to be solely... more Infertility affects around 15% of couples worldwide, and from these, males are found to be solely responsible for 23 to 30% of the cases of infertility. Cryopreservation is a common technique used to preserve male sperm and it has been widely used in assisted reproduction technology, representing the possibility of overcoming traditional obstacles to reproduction, such as age and the availability of female and male reproductive cells, and cases of pre-radiation, chemotherapy and surgical treatments. Although the very positive clinical reports and results regarding the survival of frozen and thawed cells, the freezing and inherent thawing of cells represent a dynamic process that may have a negative impact on the integrity of the sperm cells' genome. Some evidence suggests that after cryopreservation there is an increase in spermatozoa DNA breaks. The aim of the present study was to compare DNA damage of fresh and cryopreserved sperm cells in 30 men followed in the CHTMAD fertility consultation, with normal seminal parameters (according to WHO, 2010). DNA breaks were evaluated by alkaline Comet assay on samples, before and after cryopreservation in liquid nitrogen (LN). Damage were evaluated by visual score, that classify damage between 0 and 400 arbitrary units (AU). The DNA damage observed in fresh samples was very variable (from 11 AU to 372 AU) and the damage in cryopreserved samples was always greater than the damage presents in the fresh samples (266 AU to 390 AU). The mean difference between them was 185.8 AU. Despite the reduced number of samples, these results suggest that cryopreservation increase DNA damage. Since DNA damage is associated with reduced fertilization rates more studies are needed in order to fully comprehend the role of cryopreservation in sperm cells.
Nascer e Crescer, Birth and Growth Medical Journal. Vol. XXVI, Suplement II. S30. , 2017
Infertility affects around 15% of couples worldwide, and from these, males are found to be solely... more Infertility affects around 15% of couples worldwide, and from these, males are found to be solely responsible for 23 to 30% of the cases of infertility. Cryopreservation is a common technique used to preserve male sperm and it has been widely used in assisted reproduction technology, representing the possibility of overcoming traditional obstacles to reproduction, such as age and the availability of female and male reproductive cells, and cases of pre-radiation, chemotherapy and surgical treatments. Although the very positive clinical reports and results regarding the survival of frozen and thawed cells, the freezing and inherent thawing of cells represent a dynamic process that may have a negative impact on the integrity of the sperm cells' genome. Some evidence suggests that after cryopreservation there is an increase in spermatozoa DNA breaks. The aim of the present study was to compare DNA damage of fresh and cryopreserved sperm cells in 30 men followed in the CHTMAD fertility consultation, with normal seminal parameters (according to WHO, 2010). DNA breaks were evaluated by alkaline Comet assay on samples, before and after cryopreservation in liquid nitrogen (LN). Damage were evaluated by visual score, that classify damage between 0 and 400 arbitrary units (AU). The DNA damage observed in fresh samples was very variable (from 11 AU to 372 AU) and the damage in cryopreserved samples was always greater than the damage presents in the fresh samples (266 AU to 390 AU). The mean difference between them was 185.8 AU. Despite the reduced number of samples, these results suggest that cryopreservation increase DNA damage. Since DNA damage is associated with reduced fertilization rates more studies are needed in order to fully comprehend the role of cryopreservation in sperm cells.
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Conference Presentations by Joana Matos
The aim of the present study was to compare DNA damage of fresh and cryopreserved sperm cells in 30 men followed in the CHTMAD fertility consultation, with normal seminal parameters (according to WHO, 2010).
DNA breaks were evaluated by alkaline Comet assay on samples, before and after cryopreservation in liquid nitrogen (LN). Damage were evaluated by visual score, that classify damage between 0 and 400 arbitrary units (AU).
The DNA damage observed in fresh samples was very variable (from 11 AU to 372 AU) and the damage in cryopreserved samples was always greater than the damage presents in the fresh samples (266 AU to 390 AU). The mean difference between them was 185.8 AU.
Despite the reduced number of samples, these results suggest that cryopreservation increase DNA damage. Since DNA damage is associated with reduced fertilization rates more studies are needed in order to fully comprehend the role of cryopreservation in sperm cells.
The aim of the present study was to compare DNA damage of fresh and cryopreserved sperm cells in 30 men followed in the CHTMAD fertility consultation, with normal seminal parameters (according to WHO, 2010).
DNA breaks were evaluated by alkaline Comet assay on samples, before and after cryopreservation in liquid nitrogen (LN). Damage were evaluated by visual score, that classify damage between 0 and 400 arbitrary units (AU).
The DNA damage observed in fresh samples was very variable (from 11 AU to 372 AU) and the damage in cryopreserved samples was always greater than the damage presents in the fresh samples (266 AU to 390 AU). The mean difference between them was 185.8 AU.
Despite the reduced number of samples, these results suggest that cryopreservation increase DNA damage. Since DNA damage is associated with reduced fertilization rates more studies are needed in order to fully comprehend the role of cryopreservation in sperm cells.