ABSTRACT The genus Passiflora comprises hundred species, mainly native of the South American trop... more ABSTRACT The genus Passiflora comprises hundred species, mainly native of the South American tropics and rainforests, which are grouped into 21 subgenera. Some species are widely studied for their economic importance and are chiefly cultivated for production of fruit juice. To obtain a continuous source of material for a screening of secondary metabolites, zygotic embryo culture was attempted for 62 Passiflora species, starting from seeds mainly collected in the wild. Twenty nine of these species produced calli, which had very different growth rates. Plants were successfully regenerated from calli of 13 different species. For 25 of the responsive species this is the first report of in vitro culture.
A metabolomic approach followed by principal components and partial least square analysis was use... more A metabolomic approach followed by principal components and partial least square analysis was used for investigating the effect of environmental factors on two Daucus carota L. cv. Flakkese cell lines (R3M and R4G), selected for their ability to produce anthocyanins in the light and the dark, respectively. A positive correlation between total anthocyanin, hydroxycinnamic and hydroxybenzoic acid accumulation was found in both lines. Furthermore, the experimental design and the combination of biochemical and statistical analyses allowed us to unravel complex relationships between environmental factors and phenylpropanoid composition. Among these, the induction by mechanical stress of overproduction of all anthocyanins, hydroxycinnamic and hydroxybenzoic acids except sinapic acid derivatives, whose accumulation was inhibited.
Echinacea angustifolia cell suspension cultures are usually grown and maintained in the dark, but... more Echinacea angustifolia cell suspension cultures are usually grown and maintained in the dark, but we also exposed cells to light for one culture cycle (14 days) and then compared the metabolomes of dark-grown and illuminated cells by liquid chromatography-mass spectrometry. Among 256 signals, we putatively identified 159 molecules corresponding to 56 different metabolites plus their fragments, adducts and isotopologs. The E. angustifolia metabolome consisted mainly of caffeic acid derivatives, comprising (a) caffeic acid conjugated with tartaric, quinic and hexaric acids; and (b) caffeic acid conjugated with hydroxytyrosol glycosides (e.g., echinacoside, verbascoside and related molecules). Many of these metabolites have not been previously described in E. angustifolia, which currently lacks detailed metabolic profiles. Exposure to light significantly increased the levels of certain caffeic acid derivatives (particularly caffeoylquinic acids and hydroxytyrosol derivatives lacking rhamnose residues) and reduced the level of hydroxytyrosol derivatives with rhamnose residues, revealing that light specifically inhibits the rhamnosylation of caffeoyl phenylethanoid glycosides. These results are significant because they suggest that the metabolic profile of cell cultures can be manipulated by controlling simple environmental variables such as illumination to modulate the levels of potentially therapeutic compounds.
Microscopic and flow cytometric analysis of protoplasts derived from carrot (Daucus carota L.) ce... more Microscopic and flow cytometric analysis of protoplasts derived from carrot (Daucus carota L.) cells in culture revealed two different cellular subpopulations that differ in their light-scattering properties and in the emission of red autofluorescence. The two subpopulations were sorted on the basis of scattering properties and cultured independently. Subpopulation 1, characterized by higher side scattering (SS) values, had a higher
... of meristematic pea root tissue for biochemical and flow cytometry applications Donato Chiata... more ... of meristematic pea root tissue for biochemical and flow cytometry applications Donato Chiatante, Paola Brusa, Marisa Levi, Sergio Sgorbati and Elio Sparvoli ... This hole had a rim to hold a rubberring. Two Miracloth layers, held in place by the rubber ring, closed the hole. ...
Page 1. PHYSIOL. PLANT. 51: 321-325. Copenhagen 1981 Lack of correlation between overall protein ... more Page 1. PHYSIOL. PLANT. 51: 321-325. Copenhagen 1981 Lack of correlation between overall protein synthesis and the onset of cell elongation in germinating embryos of Haplopappus gracilis Maria Grazia Galli, Marisa Levi and Elio Sparvoli ...
Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparati... more Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cells. Compared with previous results obtained by using a Sephadex LH-20 column, SPE coupled with NMR spectroscopy improves the analysis of aromatic compounds e.g.: trans-feruloyl derivatives and trans-coumaroyl derivatives. Moreover, it decreases the concentration of sugars that usually overlap with many plant metabolite signals.
ABSTRACT The genus Passiflora comprises hundred species, mainly native of the South American trop... more ABSTRACT The genus Passiflora comprises hundred species, mainly native of the South American tropics and rainforests, which are grouped into 21 subgenera. Some species are widely studied for their economic importance and are chiefly cultivated for production of fruit juice. To obtain a continuous source of material for a screening of secondary metabolites, zygotic embryo culture was attempted for 62 Passiflora species, starting from seeds mainly collected in the wild. Twenty nine of these species produced calli, which had very different growth rates. Plants were successfully regenerated from calli of 13 different species. For 25 of the responsive species this is the first report of in vitro culture.
A metabolomic approach followed by principal components and partial least square analysis was use... more A metabolomic approach followed by principal components and partial least square analysis was used for investigating the effect of environmental factors on two Daucus carota L. cv. Flakkese cell lines (R3M and R4G), selected for their ability to produce anthocyanins in the light and the dark, respectively. A positive correlation between total anthocyanin, hydroxycinnamic and hydroxybenzoic acid accumulation was found in both lines. Furthermore, the experimental design and the combination of biochemical and statistical analyses allowed us to unravel complex relationships between environmental factors and phenylpropanoid composition. Among these, the induction by mechanical stress of overproduction of all anthocyanins, hydroxycinnamic and hydroxybenzoic acids except sinapic acid derivatives, whose accumulation was inhibited.
Echinacea angustifolia cell suspension cultures are usually grown and maintained in the dark, but... more Echinacea angustifolia cell suspension cultures are usually grown and maintained in the dark, but we also exposed cells to light for one culture cycle (14 days) and then compared the metabolomes of dark-grown and illuminated cells by liquid chromatography-mass spectrometry. Among 256 signals, we putatively identified 159 molecules corresponding to 56 different metabolites plus their fragments, adducts and isotopologs. The E. angustifolia metabolome consisted mainly of caffeic acid derivatives, comprising (a) caffeic acid conjugated with tartaric, quinic and hexaric acids; and (b) caffeic acid conjugated with hydroxytyrosol glycosides (e.g., echinacoside, verbascoside and related molecules). Many of these metabolites have not been previously described in E. angustifolia, which currently lacks detailed metabolic profiles. Exposure to light significantly increased the levels of certain caffeic acid derivatives (particularly caffeoylquinic acids and hydroxytyrosol derivatives lacking rhamnose residues) and reduced the level of hydroxytyrosol derivatives with rhamnose residues, revealing that light specifically inhibits the rhamnosylation of caffeoyl phenylethanoid glycosides. These results are significant because they suggest that the metabolic profile of cell cultures can be manipulated by controlling simple environmental variables such as illumination to modulate the levels of potentially therapeutic compounds.
Microscopic and flow cytometric analysis of protoplasts derived from carrot (Daucus carota L.) ce... more Microscopic and flow cytometric analysis of protoplasts derived from carrot (Daucus carota L.) cells in culture revealed two different cellular subpopulations that differ in their light-scattering properties and in the emission of red autofluorescence. The two subpopulations were sorted on the basis of scattering properties and cultured independently. Subpopulation 1, characterized by higher side scattering (SS) values, had a higher
... of meristematic pea root tissue for biochemical and flow cytometry applications Donato Chiata... more ... of meristematic pea root tissue for biochemical and flow cytometry applications Donato Chiatante, Paola Brusa, Marisa Levi, Sergio Sgorbati and Elio Sparvoli ... This hole had a rim to hold a rubberring. Two Miracloth layers, held in place by the rubber ring, closed the hole. ...
Page 1. PHYSIOL. PLANT. 51: 321-325. Copenhagen 1981 Lack of correlation between overall protein ... more Page 1. PHYSIOL. PLANT. 51: 321-325. Copenhagen 1981 Lack of correlation between overall protein synthesis and the onset of cell elongation in germinating embryos of Haplopappus gracilis Maria Grazia Galli, Marisa Levi and Elio Sparvoli ...
Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparati... more Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cells. Compared with previous results obtained by using a Sephadex LH-20 column, SPE coupled with NMR spectroscopy improves the analysis of aromatic compounds e.g.: trans-feruloyl derivatives and trans-coumaroyl derivatives. Moreover, it decreases the concentration of sugars that usually overlap with many plant metabolite signals.
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