Environmental toxicology and chemistry, Jan 11, 2017
Glyphosate is currently the most widely used herbicide in agricultural production. It generally e... more Glyphosate is currently the most widely used herbicide in agricultural production. It generally enters aquatic ecosystems through surface water runoff and aerial drift. We evaluated the effect of glyphosate acid on biochemical parameters of periphyton exposed to concentrations of 1, 3 and 6 mg/L in outdoor mesocosms, in the presence and absence of the mussel Limnoperna fortunei. Periphyton ash-free dry weight (AFDW), chlorophyll a content, carotene/chlorophyll a ratio, lipid peroxidation levels and superoxide dismutase and catalase activities were determined at day 0, 1, 7, 14 and 26 of the experimental period. AFDW was similar between control and glyphosate-treated periphyton in the absence of L. fortunei. The latter had significantly lower carotene/chlorophyll a ratio and enzyme activities and higher lipid peroxidation levels and chlorophyll a content than the former. These results show an adverse effect of glyphosate on the metabolism of periphyton community organisms, possibly i...
The effects of a commercial glyphosate formulation on the oxidative stress parameters and morphol... more The effects of a commercial glyphosate formulation on the oxidative stress parameters and morphology (in-cluding the ultrastructure) of the phytoplanktonic green microalga Scenedesmus vacuolatus were evaluated. After 96 h of exposure to increasing herbicide concentrations (0, 4, 6, 8 mg L −1 active ingredient) with the addition of alkyl aryl polyglycol ether surfactant, the growth of the cultures decreased (96 h-IC50-4.90 mg L −1) and metabolic and morphology alterations were observed. Significant increases in cellular volume (103-353%) and dry weight (105%) and a significant decrease in pigment content (41-48%) were detected. Oxidative stress parameters were significantly affected, showing an increase in the reactive oxygen species (ROS) and reduced glu-tathione (GSH) contents, oxidative damage to lipids and proteins and a decrease in the activities of the anti-oxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and the detoxifying enzyme glutathione-S-transferase (GST). Cells exposed to glyphosate formulation were larger and showed an increase in vacuole size, bleaching, cell wall thickening and alteration of the stacking pattern of thylakoids. The results of this study showed the participation of oxidative stress in the mechanism of toxic action of the commercial glyphosate formulation on S. vacuolatus and the relation between the biochemical, morphological and ultrastructure alterations .
Ecotoxicology and environmental safety, Jan 21, 2018
The aim of this study was to analyze the biochemical alterations in the golden mussel Limnoperna ... more The aim of this study was to analyze the biochemical alterations in the golden mussel Limnoperna fortunei under dietary glyphosate exposure. Mussels were fed during 4 weeks with the green algae Scenedesmus vacuolatus previously exposed to a commercial formulation of glyphosate (6 mg L active principle) with the addition of alkyl aryl polyglycol ether surfactant. After 1, 7, 14, 21 and 28 days of dietary exposure, glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), acetylcholinesterase (AChE), carboxylesterases (CES) and alkaline phosphatase (ALP) activities, glutathione (GSH) content and damage to lipids and proteins levels were analyzed. A significant increase (72%) in the GST activity and a significant decrease (26%) in the CES activity in the mussels fed on glyphosate exposed algae for 28 days were observed. The ALP activity was significantly increased at 21 and 28 days of dietary exposure (48% and 72%, respectively). GSH content and CAT, SOD and AchE act...
Environmental toxicology and chemistry, Jan 11, 2017
Glyphosate is currently the most widely used herbicide in agricultural production. It generally e... more Glyphosate is currently the most widely used herbicide in agricultural production. It generally enters aquatic ecosystems through surface water runoff and aerial drift. We evaluated the effect of glyphosate acid on biochemical parameters of periphyton exposed to concentrations of 1, 3 and 6 mg/L in outdoor mesocosms, in the presence and absence of the mussel Limnoperna fortunei. Periphyton ash-free dry weight (AFDW), chlorophyll a content, carotene/chlorophyll a ratio, lipid peroxidation levels and superoxide dismutase and catalase activities were determined at day 0, 1, 7, 14 and 26 of the experimental period. AFDW was similar between control and glyphosate-treated periphyton in the absence of L. fortunei. The latter had significantly lower carotene/chlorophyll a ratio and enzyme activities and higher lipid peroxidation levels and chlorophyll a content than the former. These results show an adverse effect of glyphosate on the metabolism of periphyton community organisms, possibly i...
The effects of a commercial glyphosate formulation on the oxidative stress parameters and morphol... more The effects of a commercial glyphosate formulation on the oxidative stress parameters and morphology (in-cluding the ultrastructure) of the phytoplanktonic green microalga Scenedesmus vacuolatus were evaluated. After 96 h of exposure to increasing herbicide concentrations (0, 4, 6, 8 mg L −1 active ingredient) with the addition of alkyl aryl polyglycol ether surfactant, the growth of the cultures decreased (96 h-IC50-4.90 mg L −1) and metabolic and morphology alterations were observed. Significant increases in cellular volume (103-353%) and dry weight (105%) and a significant decrease in pigment content (41-48%) were detected. Oxidative stress parameters were significantly affected, showing an increase in the reactive oxygen species (ROS) and reduced glu-tathione (GSH) contents, oxidative damage to lipids and proteins and a decrease in the activities of the anti-oxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and the detoxifying enzyme glutathione-S-transferase (GST). Cells exposed to glyphosate formulation were larger and showed an increase in vacuole size, bleaching, cell wall thickening and alteration of the stacking pattern of thylakoids. The results of this study showed the participation of oxidative stress in the mechanism of toxic action of the commercial glyphosate formulation on S. vacuolatus and the relation between the biochemical, morphological and ultrastructure alterations .
Ecotoxicology and environmental safety, Jan 21, 2018
The aim of this study was to analyze the biochemical alterations in the golden mussel Limnoperna ... more The aim of this study was to analyze the biochemical alterations in the golden mussel Limnoperna fortunei under dietary glyphosate exposure. Mussels were fed during 4 weeks with the green algae Scenedesmus vacuolatus previously exposed to a commercial formulation of glyphosate (6 mg L active principle) with the addition of alkyl aryl polyglycol ether surfactant. After 1, 7, 14, 21 and 28 days of dietary exposure, glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), acetylcholinesterase (AChE), carboxylesterases (CES) and alkaline phosphatase (ALP) activities, glutathione (GSH) content and damage to lipids and proteins levels were analyzed. A significant increase (72%) in the GST activity and a significant decrease (26%) in the CES activity in the mussels fed on glyphosate exposed algae for 28 days were observed. The ALP activity was significantly increased at 21 and 28 days of dietary exposure (48% and 72%, respectively). GSH content and CAT, SOD and AchE act...
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Papers by María Iummato