The persistent nature of heavy metals makes them hazardous in long term. The use of natural chelation by tolerant plants remains a more rational and promising way for the environment compared to the use of chemical chela-tors. Barley has... more
The persistent nature of heavy metals makes them hazardous in long term. The use of natural chelation by tolerant plants remains a more rational and promising way for the environment compared to the use of chemical chela-tors. Barley has already been used as a model plant in the case of heavy met-als pollution.The present study focuses on the effect of lead at different con-centrations (100mg/l, 500mg/l and 900mg/l) on barley Hordem vulgare L. After 30 days of growth, the plants were harvested and physio-biochemical parameters (fresh weight, length, relative water content, photosynthetic pigments, hydrogen peroxide, lipid peroxidation, total protein and activity of catalase and peroxidase) were studied in both leaves and roots. Results re-vealed that lead induced a decrease in fresh weight, length, relative water content and photosynthetic pigments in lead treated plants compared to the control, while an increase was recorded in lipid peroxidation, hydrogen per-oxide production and ...
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Previous human and experimental studies have demonstrated that lead exposure may modify the metabolism of lipid. Oxidative stress with subsequent lipid peroxidation has been postulated as one mechanism for lead toxicity. The protective... more
Previous human and experimental studies have demonstrated that lead exposure may modify the metabolism of lipid. Oxidative stress with subsequent lipid peroxidation has been postulated as one mechanism for lead toxicity. The protective action of vitamins C against lead affects lipid hydroperoxide level and liver functions in male rats has been studied. Experiments were performed on male waster rats with body weights of 120-160 g. Male wistar rats were exposed to 3 g/l lead acetate in drinking water for 5 weeks and treated thereafter with vitamin C (500 mg/kg, orally) for 28 days. One day after the feeding was over, venous blood samples, under chloroform anesthesia, were collected. The animals were killed by exsanguinations and the liver was excise for determination the metal content and histopathological changes. Similarly, the tissue lipid (lipid peroxidation) and the enzyme fraction (superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (ALP), acid phosphatase (ACP) and...
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Oxidative stress was induced by lead acetate (Pb) in Raphanus sativus seedlings grown in a hydroponic system using sand as substrate. Thirty day old acclimated seeds were treated for 7 days with five Pb levels (0 as control, 100, 200, 500... more
Oxidative stress was induced by lead acetate (Pb) in Raphanus sativus seedlings grown in a hydroponic system using sand as substrate. Thirty day old acclimated seeds were treated for 7 days with five Pb levels (0 as control, 100, 200, 500 and 1000 mg l-1). Parameters such as growth, oxidative damage markers (lipid peroxidation, protein oxidation and hydrogen peroxide contents) and enzymatic activities of catalase (CAT) and peroxidase (POD) were investigated. Lead concentration in plant tissues increased with increasing of Pb levels. Shoot fresh weight, chlorophyll and carotenoid concentration were significantly decreased at 100 mg l-1 Pb. Lipid peroxidation, protein oxidation and H2O2 levels were increased at 500 and 1000 mg l-1 Pb compared to control treatment, in shoots. Peroxidase activity showed a straight correlation with H2O2 concentration, whereas CAT activity decreased only in shoots. These changes in enzymatic and non-enzymatic antioxidants showed that the Pb exposition had...