There is no clear single factor to date that explains colony loss in bees, but one factor propose... more There is no clear single factor to date that explains colony loss in bees, but one factor proposed is the wide-spread application of agrochemicals. Concentrations of 14 organophosphorous insecticides (OPs) in bees and hive matrices (honey and pollen) were measured to assess their hazard to honey bees. Samples were collected during spring and summer of 2013, from 5 provinces in the middle delta of the Nile River, Egypt. LC/MS-MS was used to identify and quantify individual OPs by the use of a modified Quick Easy Cheap Effective Rugged Safe (QuEChERS) method. Pesticides were detected more frequently in samples collected during summer. Pollen contained the greatest concentrations of OPs. Profenofos, chlorpyrifos, malation and diazinon were the most frequently detected OPs. In contrast, ethoprop, phorate, coumaphos and chlorpyrifos-oxon were not detected. Hazard quotients (HQs) were determined by using either zero or the limit of detection as surrogate values for samples in which individual OPs were not detected for best and worst case respectively. A toxic units approach, with lethality as the endpoint was used in an additive model to assess the cumulative potential for adverse effects posed by OPs. Hazard quotients (HQs) in honey and pollen ranged from 0.1 - 0.2 during spring and from 0.2 - 0.3 during summer, respectively. HQs based on lethality due to direct exposure of adult worker bees to OPs during spring and summer ranged from 0.04 to 0.1 for best and worst case respectively. It is concluded that direct exposure and or dietary exposure to OPs in honey and pollen pose little threat due to lethality of dietary exposure of bees in Egypt.
The aim of this study is to evaluate the effectiveness of honeybees and their associated products... more The aim of this study is to evaluate the effectiveness of honeybees and their associated products as biological indicators of the presence of lead, cadmium, copper, iron and zinc in the environment. Samples were collected from four different Egyptian regions with different anthropogenic activities namely, Kafr El-Sheikh, El-Mehala El-kobra , Kafr El-Zayat and Al-Fayoum during spring and summer honey harvest 2010. Differences in the concentrations of heavy metals in fresh honey collected during spring and summer honey harvests were observed. Cadmium was found completely absent in fresh honey summer harvest collected from all apiaries. However, the values of Cd in honey samples harvested during spring from the four apiaries exceeded the maximum admitted level (0.05 ppm). In addition, the content of Pb in honey during summer exceeded the maximum admitted level (1.5 ppm) only at Kafr El-zayat. Bee gathered pollen heavy metal contents were higher during spring when compared with those du...
Concentrations of 14 organophosphorus insecticides (OPs) were measured in Apis mellifera L. (Euro... more Concentrations of 14 organophosphorus insecticides (OPs) were measured in Apis mellifera L. (European honeybee) and hive matrices (honey and bee bread). Samples were collected from seven randomly selected colonies in central Saskatchewan during the summer of 2013. LC-MS/MS was used to identify and quantify individual OP by use of a modified quick easy cheap effective rugged safe (QuEChERS) method. Diazinon, dimethoate, and chlorpyrifos-oxon were the only OPs detected in honey with mean concentrations of 0.3, 1.5, and 0.2 ng/g, wet mass (wm), respectively. Fenamiphos, chlorpyrifos, and chlorpyrifos methyl were the only OPs detected in bee bread, with mean concentrations of 0.4, 2.7, and 15.8 ng/g, wm, respectively, while ethoprop, malathion, and dichlorvos were the only OPs detected in bees with mean concentrations of 1.4, 3.7, and 889.2 ng/g, wm, respectively. Total hazard quotients (HQs), based on lethality of bees exposed to OPs in honey and pollen consumed by bees ranged from 0.01 to 0.06 and based on lethality of bees from direct exposure to OPs ranged from 0.3 to 0.4 which suggests little hazard of OPs to Saskatchewan beehives.
Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carc... more Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carcinogenic due its potential in inducing the oxidative stress and distortion of the most antioxidant enzymes. Since black tea possesses strong antioxidant activity, it protects cells and tissues against oxidative stress. Curcumin (CMN), a naturally occurring agent, has a combination of biological and pharmacological properties that include antioxidant activity. Therefore, the present study was carried out to investigate the possible role of separate and mixed supplementation of black tea extract and CMN in the hepatotoxicity induced by AFB1 in rats. A total of 48 adult male Sprague Dawley rats were randomly divided into eight groups with six rats in each group. Group 1 (normal control) includes rats that received no treatment. Groups 2, 3, and 4 (positive control) include rats that received olive oil, black tea extract, and CMN, respectively. Group 5 includes rats that received AFB1 at a d...
Ecotoxicology and environmental safety, Jan 6, 2015
There is no clear single factor to date that explains colony loss in bees, but one factor propose... more There is no clear single factor to date that explains colony loss in bees, but one factor proposed is the wide-spread application of agrochemicals. Concentrations of 14 organophosphorous insecticides (OPs) in honey bees (Apis mellifera) and hive matrices (honey and pollen) were measured to assess their hazard to honey bees. Samples were collected during spring and summer of 2013, from 5 provinces in the middle delta of Egypt. LC/MS-MS was used to identify and quantify individual OPs by use of a modified Quick Easy Cheap Effective Rugged Safe (QuEChERS) method. Pesticides were detected more frequently in samples collected during summer. Pollen contained the greatest concentrations of OPs. Profenofos, chlorpyrifos, malation and diazinon were the most frequently detected OPs. In contrast, ethoprop, phorate, coumaphos and chlorpyrifos-oxon were not detected. A toxic units approach, with lethality as the endpoint was used in an additive model to assess the cumulative potential for advers...
There is no clear single factor to date that explains colony loss in bees, but one factor propose... more There is no clear single factor to date that explains colony loss in bees, but one factor proposed is the wide-spread application of agrochemicals. Concentrations of 14 organophosphorous insecticides (OPs) in bees and hive matrices (honey and pollen) were measured to assess their hazard to honey bees. Samples were collected during spring and summer of 2013, from 5 provinces in the middle delta of the Nile River, Egypt. LC/MS-MS was used to identify and quantify individual OPs by the use of a modified Quick Easy Cheap Effective Rugged Safe (QuEChERS) method. Pesticides were detected more frequently in samples collected during summer. Pollen contained the greatest concentrations of OPs. Profenofos, chlorpyrifos, malation and diazinon were the most frequently detected OPs. In contrast, ethoprop, phorate, coumaphos and chlorpyrifos-oxon were not detected. Hazard quotients (HQs) were determined by using either zero or the limit of detection as surrogate values for samples in which individual OPs were not detected for best and worst case respectively. A toxic units approach, with lethality as the endpoint was used in an additive model to assess the cumulative potential for adverse effects posed by OPs. Hazard quotients (HQs) in honey and pollen ranged from 0.1 - 0.2 during spring and from 0.2 - 0.3 during summer, respectively. HQs based on lethality due to direct exposure of adult worker bees to OPs during spring and summer ranged from 0.04 to 0.1 for best and worst case respectively. It is concluded that direct exposure and or dietary exposure to OPs in honey and pollen pose little threat due to lethality of dietary exposure of bees in Egypt.
The aim of this study is to evaluate the effectiveness of honeybees and their associated products... more The aim of this study is to evaluate the effectiveness of honeybees and their associated products as biological indicators of the presence of lead, cadmium, copper, iron and zinc in the environment. Samples were collected from four different Egyptian regions with different anthropogenic activities namely, Kafr El-Sheikh, El-Mehala El-kobra , Kafr El-Zayat and Al-Fayoum during spring and summer honey harvest 2010. Differences in the concentrations of heavy metals in fresh honey collected during spring and summer honey harvests were observed. Cadmium was found completely absent in fresh honey summer harvest collected from all apiaries. However, the values of Cd in honey samples harvested during spring from the four apiaries exceeded the maximum admitted level (0.05 ppm). In addition, the content of Pb in honey during summer exceeded the maximum admitted level (1.5 ppm) only at Kafr El-zayat. Bee gathered pollen heavy metal contents were higher during spring when compared with those du...
Concentrations of 14 organophosphorus insecticides (OPs) were measured in Apis mellifera L. (Euro... more Concentrations of 14 organophosphorus insecticides (OPs) were measured in Apis mellifera L. (European honeybee) and hive matrices (honey and bee bread). Samples were collected from seven randomly selected colonies in central Saskatchewan during the summer of 2013. LC-MS/MS was used to identify and quantify individual OP by use of a modified quick easy cheap effective rugged safe (QuEChERS) method. Diazinon, dimethoate, and chlorpyrifos-oxon were the only OPs detected in honey with mean concentrations of 0.3, 1.5, and 0.2 ng/g, wet mass (wm), respectively. Fenamiphos, chlorpyrifos, and chlorpyrifos methyl were the only OPs detected in bee bread, with mean concentrations of 0.4, 2.7, and 15.8 ng/g, wm, respectively, while ethoprop, malathion, and dichlorvos were the only OPs detected in bees with mean concentrations of 1.4, 3.7, and 889.2 ng/g, wm, respectively. Total hazard quotients (HQs), based on lethality of bees exposed to OPs in honey and pollen consumed by bees ranged from 0.01 to 0.06 and based on lethality of bees from direct exposure to OPs ranged from 0.3 to 0.4 which suggests little hazard of OPs to Saskatchewan beehives.
Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carc... more Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carcinogenic due its potential in inducing the oxidative stress and distortion of the most antioxidant enzymes. Since black tea possesses strong antioxidant activity, it protects cells and tissues against oxidative stress. Curcumin (CMN), a naturally occurring agent, has a combination of biological and pharmacological properties that include antioxidant activity. Therefore, the present study was carried out to investigate the possible role of separate and mixed supplementation of black tea extract and CMN in the hepatotoxicity induced by AFB1 in rats. A total of 48 adult male Sprague Dawley rats were randomly divided into eight groups with six rats in each group. Group 1 (normal control) includes rats that received no treatment. Groups 2, 3, and 4 (positive control) include rats that received olive oil, black tea extract, and CMN, respectively. Group 5 includes rats that received AFB1 at a d...
Ecotoxicology and environmental safety, Jan 6, 2015
There is no clear single factor to date that explains colony loss in bees, but one factor propose... more There is no clear single factor to date that explains colony loss in bees, but one factor proposed is the wide-spread application of agrochemicals. Concentrations of 14 organophosphorous insecticides (OPs) in honey bees (Apis mellifera) and hive matrices (honey and pollen) were measured to assess their hazard to honey bees. Samples were collected during spring and summer of 2013, from 5 provinces in the middle delta of Egypt. LC/MS-MS was used to identify and quantify individual OPs by use of a modified Quick Easy Cheap Effective Rugged Safe (QuEChERS) method. Pesticides were detected more frequently in samples collected during summer. Pollen contained the greatest concentrations of OPs. Profenofos, chlorpyrifos, malation and diazinon were the most frequently detected OPs. In contrast, ethoprop, phorate, coumaphos and chlorpyrifos-oxon were not detected. A toxic units approach, with lethality as the endpoint was used in an additive model to assess the cumulative potential for advers...
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Papers by Mohamed Mona