Asian Pacific journal of cancer prevention : APJCP, 2016
Cancer represent one of the most serious health problems and major causes of death around the wor... more Cancer represent one of the most serious health problems and major causes of death around the world. Many anticancer drugs in clinical use today are natural products or derived from natural sources. Withania somnifera (L.) Dunal is a small shrub widely distributed in many parts of the world including Saudi Arabia. The antiproliferative activities of the methanolic extract of W. somnifera leaves collected from Faifa mountains, southwest Saudi Arabia against MCF-7, HCT116 and HepH2 cell lines were investigated. The extract showed a strong antiproliferative activity against all cell lines with IC50 values of 3.35, 2.19 and 1.89 μg/ml, respectively. Flow cytometry results showed that the extract arrested the cell cycle at S phase, and the increase in the caspase 3 activity suggested that the extract could induce cell apoptosis by a caspase mediated pathway. These results demonstrated that the methanolic extract of W. somnifera leaves collected from Faifa mountains has comparable strong ...
ology & molecular biology journal is one of the series issued twice by the Egyptian Physi Academi... more ology & molecular biology journal is one of the series issued twice by the Egyptian Physi Academic Journal of Biological Sciences, and is devoted to publication of original papers that siological elucidate important biological, chemical, or physical mechanisms of broad phy significance. www.eajbs.eg.net Provided for non-commercial research and education use. Not for reproduction, distribution or commercial use.
Dimpylate is one of the most organophosphorus widely used insecticides in agriculture. This study... more Dimpylate is one of the most organophosphorus widely used insecticides in agriculture. This study aims to investigate the ameliorative effect of German chamomile (Matricaria recutita) on the hepato-nephrotoxicity induced by Dimpylate in male Wistar rats. Rats were divided into 4 groups: Control group, received corn oil alone; Chamomile group, orally given water extract of Chamomile (300 mg/kg b.wt./day for 30 days); Dimpylate group, orally given 15 mg/kg b.wt./day for 30 days of Dimpylate; and Dimpylate and chamomile group, orally given Dimpylate (15 mg/kg b.wt./day) with Chamomile extract (300 mg/kg b.wt./day) for 30 days. Oxidative stress and antioxidant status were estimated in the liver and kidney of all groups. In the liver and kidney of the Dimpylate-intoxicated rats, there was an increase in malondialdehyde (MDA) concentration and a significant decrease in the activities of superoxide dismutase (SOD), total antioxidant capacity (TCA), glutathione-peroxidase (GPx), glutathione...
This study was conducted to assess the effect of ginger (Zingiber officinale) aqueous extract, on... more This study was conducted to assess the effect of ginger (Zingiber officinale) aqueous extract, on the oxidative status, antioxidant defense system and liver pathology of Schistosoma mansoni -infected C57BL/6 mice. Ginger at dose level of 500 mg/kg body weight was orally administered, daily for five weeks from the 5 th week post-infection. Result showed that S. mansoni-infected mice exhibited a suppression of liver antioxidant capacity, and depleted reduced glutathione content (GSH), superoxide dismutase (SOD), and catalase (CAT) activities. In addition, the hepatic lipid peroxidation was deleteriously elevated in S. mansoni-infected mice. The hepatic total protein (TP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were profoundly decreased due to their release from necrotic liver cells into blood of S. mansoni-infected mice. Concomitantly, histopathologiacl and histochemical data indicated severe hepatic cell necrosis and multigranulomas with differ...
To study the biochemical and molecular hepatotoxicity induced by aluminium chloride (AlCl3) and t... more To study the biochemical and molecular hepatotoxicity induced by aluminium chloride (AlCl3) and the protective role of saffron and honey against such toxicity. This study was performed in the Department of Biology, College of Science, King Khalid University, Abha, Kingdom of Saudi Arabia between July and August 2009. Two mice strains, BALB/c and C57BL/6 (20 animals from each strain), were used and randomly divided into 4 groups: control group; AlCl3 group; AlCl3+saffron group; and AlCl3+honey group. Changes in liver biochemical markers such as gamma-glutamyl transpeptidase (GGT), alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin and lipid peroxidation levels were estimated. Induced and suppressed mRNA in the liver homogenate was scanned followed by up- and down- regulated genes were isolated, cloned, and sequenced. There was a significant increase in the cholesterol levels, triglycerides, GGT, ALT, AST, ALP, lipid peroxidat...
Archives des sciences / éditées par la Société de physique et d'histoire naturelle de Genève
Forced swimming test (FST) is considered an animal model of depression. Sprague dawely rats were ... more Forced swimming test (FST) is considered an animal model of depression. Sprague dawely rats were divided into 4 groups (five/group); Control group; Depression group: rats were daily exposed to FST for 10 min for 15 days; Depression& rosemary group: rats were daily exposed to FST for 10 min and orally administered with rosemary extract at 200 mg/kg b. wt. for 15 days; Depression& green coffee group: rats were daily exposed to FST for 10 min and orally administered with green coffee extract at 600 mg/kg for 15 days. There was a decrease in the acetylcholinesterase (AchE) activity in rats exposed to FST. Additionally, there was an imbalance of oxidants/antioxidants level in brain of depressed rats. Molecular study showed the expression of AchE gene was low but the expression of P53, Bcl-2 (B cell lymphoma 2) and interleukins (IL-4& IL-12) genes was high in the brain of depression group. Both aqueous rosemary and green coffee extracts have antidepressant effect delivered not only from t...
Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carc... more Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carcinogenic due its potential in inducing the oxidative stress and distortion of the most antioxidant enzymes. Since black tea possesses strong antioxidant activity, it protects cells and tissues against oxidative stress. Curcumin (CMN), a naturally occurring agent, has a combination of biological and pharmacological properties that include antioxidant activity. Therefore, the present study was carried out to investigate the possible role of separate and mixed supplementation of black tea extract and CMN in the hepatotoxicity induced by AFB1 in rats. A total of 48 adult male Sprague Dawley rats were randomly divided into eight groups with six rats in each group. Group 1 (normal control) includes rats that received no treatment. Groups 2, 3, and 4 (positive control) include rats that received olive oil, black tea extract, and CMN, respectively. Group 5 includes rats that received AFB1 at a d...
Asian Pacific journal of cancer prevention : APJCP, 2016
Cancer represent one of the most serious health problems and major causes of death around the wor... more Cancer represent one of the most serious health problems and major causes of death around the world. Many anticancer drugs in clinical use today are natural products or derived from natural sources. Withania somnifera (L.) Dunal is a small shrub widely distributed in many parts of the world including Saudi Arabia. The antiproliferative activities of the methanolic extract of W. somnifera leaves collected from Faifa mountains, southwest Saudi Arabia against MCF-7, HCT116 and HepH2 cell lines were investigated. The extract showed a strong antiproliferative activity against all cell lines with IC50 values of 3.35, 2.19 and 1.89 μg/ml, respectively. Flow cytometry results showed that the extract arrested the cell cycle at S phase, and the increase in the caspase 3 activity suggested that the extract could induce cell apoptosis by a caspase mediated pathway. These results demonstrated that the methanolic extract of W. somnifera leaves collected from Faifa mountains has comparable strong ...
ology & molecular biology journal is one of the series issued twice by the Egyptian Physi Academi... more ology & molecular biology journal is one of the series issued twice by the Egyptian Physi Academic Journal of Biological Sciences, and is devoted to publication of original papers that siological elucidate important biological, chemical, or physical mechanisms of broad phy significance. www.eajbs.eg.net Provided for non-commercial research and education use. Not for reproduction, distribution or commercial use.
Dimpylate is one of the most organophosphorus widely used insecticides in agriculture. This study... more Dimpylate is one of the most organophosphorus widely used insecticides in agriculture. This study aims to investigate the ameliorative effect of German chamomile (Matricaria recutita) on the hepato-nephrotoxicity induced by Dimpylate in male Wistar rats. Rats were divided into 4 groups: Control group, received corn oil alone; Chamomile group, orally given water extract of Chamomile (300 mg/kg b.wt./day for 30 days); Dimpylate group, orally given 15 mg/kg b.wt./day for 30 days of Dimpylate; and Dimpylate and chamomile group, orally given Dimpylate (15 mg/kg b.wt./day) with Chamomile extract (300 mg/kg b.wt./day) for 30 days. Oxidative stress and antioxidant status were estimated in the liver and kidney of all groups. In the liver and kidney of the Dimpylate-intoxicated rats, there was an increase in malondialdehyde (MDA) concentration and a significant decrease in the activities of superoxide dismutase (SOD), total antioxidant capacity (TCA), glutathione-peroxidase (GPx), glutathione...
This study was conducted to assess the effect of ginger (Zingiber officinale) aqueous extract, on... more This study was conducted to assess the effect of ginger (Zingiber officinale) aqueous extract, on the oxidative status, antioxidant defense system and liver pathology of Schistosoma mansoni -infected C57BL/6 mice. Ginger at dose level of 500 mg/kg body weight was orally administered, daily for five weeks from the 5 th week post-infection. Result showed that S. mansoni-infected mice exhibited a suppression of liver antioxidant capacity, and depleted reduced glutathione content (GSH), superoxide dismutase (SOD), and catalase (CAT) activities. In addition, the hepatic lipid peroxidation was deleteriously elevated in S. mansoni-infected mice. The hepatic total protein (TP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were profoundly decreased due to their release from necrotic liver cells into blood of S. mansoni-infected mice. Concomitantly, histopathologiacl and histochemical data indicated severe hepatic cell necrosis and multigranulomas with differ...
To study the biochemical and molecular hepatotoxicity induced by aluminium chloride (AlCl3) and t... more To study the biochemical and molecular hepatotoxicity induced by aluminium chloride (AlCl3) and the protective role of saffron and honey against such toxicity. This study was performed in the Department of Biology, College of Science, King Khalid University, Abha, Kingdom of Saudi Arabia between July and August 2009. Two mice strains, BALB/c and C57BL/6 (20 animals from each strain), were used and randomly divided into 4 groups: control group; AlCl3 group; AlCl3+saffron group; and AlCl3+honey group. Changes in liver biochemical markers such as gamma-glutamyl transpeptidase (GGT), alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin and lipid peroxidation levels were estimated. Induced and suppressed mRNA in the liver homogenate was scanned followed by up- and down- regulated genes were isolated, cloned, and sequenced. There was a significant increase in the cholesterol levels, triglycerides, GGT, ALT, AST, ALP, lipid peroxidat...
Archives des sciences / éditées par la Société de physique et d'histoire naturelle de Genève
Forced swimming test (FST) is considered an animal model of depression. Sprague dawely rats were ... more Forced swimming test (FST) is considered an animal model of depression. Sprague dawely rats were divided into 4 groups (five/group); Control group; Depression group: rats were daily exposed to FST for 10 min for 15 days; Depression& rosemary group: rats were daily exposed to FST for 10 min and orally administered with rosemary extract at 200 mg/kg b. wt. for 15 days; Depression& green coffee group: rats were daily exposed to FST for 10 min and orally administered with green coffee extract at 600 mg/kg for 15 days. There was a decrease in the acetylcholinesterase (AchE) activity in rats exposed to FST. Additionally, there was an imbalance of oxidants/antioxidants level in brain of depressed rats. Molecular study showed the expression of AchE gene was low but the expression of P53, Bcl-2 (B cell lymphoma 2) and interleukins (IL-4& IL-12) genes was high in the brain of depression group. Both aqueous rosemary and green coffee extracts have antidepressant effect delivered not only from t...
Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carc... more Aflatoxin B1 (AFB1) is a toxic compound commonly found as a contaminant in human food. It is carcinogenic due its potential in inducing the oxidative stress and distortion of the most antioxidant enzymes. Since black tea possesses strong antioxidant activity, it protects cells and tissues against oxidative stress. Curcumin (CMN), a naturally occurring agent, has a combination of biological and pharmacological properties that include antioxidant activity. Therefore, the present study was carried out to investigate the possible role of separate and mixed supplementation of black tea extract and CMN in the hepatotoxicity induced by AFB1 in rats. A total of 48 adult male Sprague Dawley rats were randomly divided into eight groups with six rats in each group. Group 1 (normal control) includes rats that received no treatment. Groups 2, 3, and 4 (positive control) include rats that received olive oil, black tea extract, and CMN, respectively. Group 5 includes rats that received AFB1 at a d...
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