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Buffelgrass (Cenchrus ciliaris) is widely grown in tropical and sub-tropical arid rangelands around the globe because of its high tolerance to drought and high level of nutrients. In the present study, the seed germination response of 12... more
Buffelgrass (Cenchrus ciliaris) is widely grown in tropical and sub-tropical arid rangelands around the globe because of its high tolerance to drought and high level of nutrients. In the present study, the seed germination response of 12 buffelgrass genotypes (nine collected accessions and three introduced varieties) was tested to record the various effects of storage duration and temperature treatments on germination. Five constant temperature treatments (15, 20, 25, 30 and 35°C) and two storage durations (12 and 24 months) were used. The studied buffelgrass genotypes responded differently to the different storage durations and temperature regimes, suggesting the importance of genotype assessment under different environments. The highest germination percentages were recorded for genotype BUR-1 at 20°C (45%) after 12 months. No germination occurred after 12 months at low constant temperatures (15°C) for all genotypes except BUR-1, BUR-2 and UIA. Therefore, there is a large genetic v...
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Rusts are a group of major diseases that have an adverse effect on crop production. Those targeting wheat are found in three principal forms: leaf, stripe, and stem rust. Leaf rust causes foliar disease in wheat; in Egypt, this causes a... more
Rusts are a group of major diseases that have an adverse effect on crop production. Those targeting wheat are found in three principal forms: leaf, stripe, and stem rust. Leaf rust causes foliar disease in wheat; in Egypt, this causes a significant annual yield loss. The deployment of resistant genotypes has proved to be a relatively economical and environmentally sustainable method of controlling the disease. Gene pyramiding can be performed using traditional breeding techniques. Additionally, pathotypes can be introduced to examine specific leaf rust genes, or the breeder may conduct more complex breeding methods. Indirect selection via DNA markers linked to resistance genes may facilitate the transfer of targeted genes, either individually or in combination, even in a disease-free environment. The use of selective crosses to counter virulent races of leaf, stripe, and stem rust has resulted in the transfer of several resistance genes into new wheat germplasm from cultivated or wild species. Quantitative trait locus (QTL) technology has been adopted in a wide variety of novel approaches and is becoming increasingly recognized in wheat breeding. Moreover, several researchers have reported the transference of leaf and stripe rust resistance genes into susceptible wheat cultivars.
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Wheat cultivar ‘Yeocra Rojo’ and ten selected genotypes originating from immature embryo culture of this cultivar were evaluated for their productivity in two field experiments during 2005/2006 and 2006/2007 winter seasons. Wheat... more
Wheat cultivar ‘Yeocra Rojo’ and ten selected genotypes originating from immature embryo culture of this cultivar were evaluated for their productivity in two field experiments during 2005/2006 and 2006/2007 winter seasons. Wheat genotypes YR-9 and YR-10 exhibited the tallest plants whereas the shortest plants were those of cv. ‘Yeocra Rojo’. In general, genotypes YR-2, YR-7, and YR-10 produced the highest grain yield while YR-8 produced the lowest. YR-8 also gave the lowest harvest index. YR-7 had the most grains per spike, spike length and number of spikelets per spike compared to its parent cultivar and all other genotypes. A set of PCR-based markers for specific HMW glutenin genes encoding By-subunits were used to identify wheat genotypes carrying By genes at the Glu-B1 locus for its breadmaking quality. The presence of the gene encoding By8, which exists in the allele combination Glu-B1b (Bx7 + By8), was detected only in one genotype: YR-7. Primer pair ZSBy9aF1/R3 gave characte...
Stress induced by high temperature represents a major constraint over wheat production in many production areas. Here, the comprehensive coverage of the wheat genome achievable using single nucleotide polymorphism markers was exploited to... more
Stress induced by high temperature represents a major constraint over wheat production in many production areas. Here, the comprehensive coverage of the wheat genome achievable using single nucleotide polymorphism markers was exploited to carry out a genetic analysis targeting yield components in plants exposed to high temperature stress. The mapping population was a set of doubled haploid lines derived from a cross between the cultivars Yecora Rojo and Ksu106. Both of the parental cultivars and their derived population were tested in the field in two locations over two consecutive seasons; at each site, two sowing dates were included, with the later sowing intended to ensure that the plants were exposed to high temperature stress during the grain filling period. Composite interval mapping detected 93 quantitative trait loci influencing grain yield and some related traits, along with 20 loci associated with a ?heat susceptibility index? (HSI). The loci were distributed over all 21 o...
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Buffelgrass (Cenchrus ciliaris L. Link) is an African agamospermous grass that has been widely introduced into arid regions of the world to improve rangelands for cattle production and as a result, it has invaded adjacent habitats.... more
Buffelgrass (Cenchrus ciliaris L. Link) is an African agamospermous grass that has been widely introduced into arid regions of the world to improve rangelands for cattle production and as a result, it has invaded adjacent habitats. Cenchrus ciliaris is apomictic, a condition that is normally associated with low genetic variation within populations, but even moderate levels of genetic variation among populations could account for differences in invasiveness. We used Inter-Simple Sequence Repeat markers (ISSRs) to examine genetic variation among buffelgrass genotypes collected from nine different locations in the central region of Saudi Arabia, and three introduced cultivars. The genetic similarity coefficients among the twelve buffelgrass genotypes ranged from 0.66 to 0.94. Maximum similarity was observed between local populations. Introduced cultivars 'Biloela', 'Gayndah' and 'USA' were quite distinct from local populations. The dendogram constructed with UPG...
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Wheat is an important cereal crop, and its significance is more due to compete for dietary products in the world. Many constraints facing by the wheat crop due to environmental hazardous, biotic, abiotic stress and heavy matters factors,... more
Wheat is an important cereal crop, and its significance is more due to compete for dietary products in the world. Many constraints facing by the wheat crop due to environmental hazardous, biotic, abiotic stress and heavy matters factors, as a result, decrease the yield. Understanding the molecular mechanism related to these factors is significant to figure out genes regulate under specific conditions. Classical breeding using hybridization has been used to increase the yield but not prospered at the desired level. With the development of newly emerging technologies in biological sciences i.e., marker assisted breeding (MAB), QTLs mapping, mutation breeding, proteomics, metabolomics, next-generation sequencing (NGS), RNA_sequencing, transcriptomics, differential expression genes (DEGs), computational resources and genome editing techniques i.e. (CRISPR cas9; Cas13) advances in the field of omics. Application of new breeding technologies develops huge data; considerable development is...
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Six bread wheat genotypes were evaluated in three separate irrigation regime experiments to compare the response of agronomic performance and to identify genotypes with high yield potential under drought stress. The first irrigation... more
Six bread wheat genotypes were evaluated in three separate irrigation regime experiments to compare the response of agronomic performance and to identify genotypes with high yield potential under drought stress. The first irrigation treatment (I3) was given normal water irrigation (about 7000 m3 ha-1, according to recommendation for Qassim Region). The second (I2) and third (I1) treatments were given 2/3 and 1/3 of water amount of the first treatment, respectively. Factorial experiments in randomized complete block design with three replications were conducted during 2009/2010 and 2010/2011 seasons in the arid environment of central region of Saudi Arabia. Measurements were taken on days to heading, plant height, number of spikes m-2, number of kernels spike-1, 1000-kernel weight and grain yield. The drought susceptibility index (DSI) and water utilization efficiency (WUE) were calculated. The results revealed that effect of irrigation regime was highly significant for all traits, e...
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Background Cucurbita spp. is a main source of crypto-xanthine, zeaxanthin lutein folates, and natural poly-phenolic flavonoid compounds. Collection and conservation of genetic variability are helpful in genetic advancement programs.... more
Background Cucurbita spp. is a main source of crypto-xanthine, zeaxanthin lutein folates, and natural poly-phenolic flavonoid compounds. Collection and conservation of genetic variability are helpful in genetic advancement programs. Twenty-two pumpkin genotypes (21genotypes of Cucurbita pepo L. and one genotype of C. maxima L.) were collected from different regions of Saudi Arabia. Fifteen HFO-TAGhigh frequency oligonucleotide–targeting active gene markers were used to analyze genetic variability among 22 pumpkin genotypes. Results A total of 107 alleles were detected by the 15 HFO-TAG markers, an average of 7.133 alleles per primer. Polymorphisms were found in 102 alleles, an average of 6.866 alleles per primer. The PIC values measured from all of the HFO-TAG markers were high, and ranged from 0.8940 to 0.7225, with an average 0.8212 per marker. Conclusions The results of the cluster analysis of pumpkin genotypes were separated into seven groups according to the collection region.
Low-fertility soil is considered a major challenge for growing wheat (Triticum aestivum) in organic systems. The objective of this study was to identify spring wheat genotype(s) adapted to growing in organic systems with low-fertility... more
Low-fertility soil is considered a major challenge for growing wheat (Triticum aestivum) in organic systems. The objective of this study was to identify spring wheat genotype(s) adapted to growing in organic systems with low-fertility soil compared with conventional systems in the central region of Saudi Arabia. Organic and conventional trials were established during the 2010, 2011 and 2012 growing seasons in the arid environment of central region of Saudi Arabia. Both trails were seeded in a sandy loam soil. Eight bread wheat genotypes were evaluated for the following parameters: grain and straw yields, grain-filling rate (GFR), days to heading (DTH), days to maturity (DTM), number of kernels per spike and 1000-kernel weight. The experiment was a randomized complete block design (RCBD) with four replications. The findings showed that there were significant differences in grain and straw yields between the two systems and among genotypes. The Egyptian genotypes 'Sids 12' and...
Research Interests: Plant Biology and April
Genetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the phenotypic level using morphological characters. The results indicated that there were... more
Genetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the phenotypic level using morphological characters. The results indicated that there were considerable variations among the different studied cultivars. Cultivars Victoria, Frizia and Safaren had the highest chlorophyll content compared to the other cultivars in both seasons. The largest leaf area and leaf dry weight were measured in cultivar Safaren and Mondial in both seasons. Cultivars Aboulx and Mondia produced the highest tuber yield in both seasons. On the other hand, cultivars Victoria and Edward were characterized by their considerably lower yield than other studied cultivars. Specific gravity varied considerably among the different potato cultivars. Thirteen random decamer primers were used to amplify DNA via the polymerase chain reaction and 75 RAPDs were generated. The RAPD profiles obtained were successfully used to differentiate p...
The suitability of randomly amplified polymorphic DNA (RAPD) fingerprints as genetic markers in date palms was tested. Five date palm cultivars (Barbi, Nabtet Ali. Rothanah, Ajwa, and Sokkari) from Saudi well- known dates were subject to... more
The suitability of randomly amplified polymorphic DNA (RAPD) fingerprints as genetic markers in date palms was tested. Five date palm cultivars (Barbi, Nabtet Ali. Rothanah, Ajwa, and Sokkari) from Saudi well- known dates were subject to DNA fingerprint analysis. From 20 primers tested, only 12 were selected as reproducible, giving 64 bands. The RAPD profiles obtained were successfully used to differentiate the genotypes. Based on the pair-wise comparison of amplification products, the genetic similarity was estimated. The five date palm cultivars showed variation at the DNA level. The genetic similarity among all date palm cultivars ranged from 70 to 85%. Sokkary was quite distant from Haiti and Ajwa cultivats. A dendrogram was constructed using UPGMA analysis. On the basis of this analysis, the populations were clustered into two clusters: cluster l contained Barhi and Ajwa cultivars, and cluster II contained Nabtet Ali, Rothanah and Sokkari cultivars. Therefore, the polymorphism ...
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Pasture grass cultivars grown for forage production in the central region of Saudi Arabia, which is dominated with desert environment, suffer a great deal of heat stress. These growing conditions greatly affect species performance and... more
Pasture grass cultivars grown for forage production in the central region of Saudi Arabia, which is dominated with desert environment, suffer a great deal of heat stress. These growing conditions greatly affect species performance and forage quality. This study was focused on evaluating productivity and forage quality of selected new pasture grass cultivars under heat stress in the central region of Saudi Arabia over a two-year period and investigating the presence of the dehydrin genes as a marker of heat tolerance in grass cultivars. Six grass cultivars were used in this study: two perennial ryegrass (Lolium perenne L.) cultivars (Aries and Quartet), two endophyte-free tall fescue (Festuca arundinacea Schreb.) cultivars (Fawn and K5666v), and two orchardgrass (Dactylis glomerata L.) cultivars (Tekapo and Niva). The results clearly showed variation in the total dry matter yield (DMY) and forage quality among the four grass cultivars. Although Fawn tall fescue showed superiority ove...
Thirteen lettuce genotypes belonging to four types (butterhead, Cos/Romaine, crisphead, and stem lettuce) were investigated using RAPD fingerprints as genetic marker to assess genetic diversity. Also, the presence of nitrate reductase... more
Thirteen lettuce genotypes belonging to four types (butterhead, Cos/Romaine, crisphead, and stem lettuce) were investigated using RAPD fingerprints as genetic marker to assess genetic diversity. Also, the presence of nitrate reductase gene (NR) in lettuce genotypes was investigated by PCR analysis. From 30 random primers tested, only 13 were selected as reproducible, giving 83 bands. The genetic similarity was estimated based on the pair-wise comparison of amplification products. The thirteen lettuce genotypes showed variation at the DNA level. The UPGMA cluster analysis separated the 13 lettuce genotypes into two distinct groups. Moreover, gene-specific primer pairs for amplification of nitrate reductase revealed the presence of nitrate reductase gene in 'Ambassador', 'Bath', 'Merveille des Quatre', 'Romain de Benicardo', 'Colona' and 'Chinese Stem' genotypes only. The work presented in this paper illustrated that sensitive specific P...
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Ahmed A.M. Dawabah*, Ahmad S. Al-Hazmi, Soloiman M. Al-Rehiayani, Ahmed L. Abdel-Mawgood,Mohamed I. Motawei, Soleman Al-Otayk, Monther T. Sadder, Abdallah M. Elgorban, Hussein M. Migdadi, Khaled A.Moustafa and Abdullah A. Al-Doss (2012). Molecular and molecular characterization of cereal cyst nem...more
The morphological and molecular characteristics of four cereal cyst nematode (Heterodera avenae) populations collected from the Qassim, Tabouk, Riyadh, and Hail regions, Saudi Arabia were comparatively investigated. A large number of soil... more
The morphological and molecular characteristics of four cereal cyst nematode (Heterodera avenae) populations collected from the Qassim, Tabouk, Riyadh, and Hail regions, Saudi Arabia were comparatively investigated. A large number of soil samples were collected from a representative field (72 ha) in each region. The morphological and morphometric characteristics of the populations were determined. Morphometric data were subjected to multivariate canonical discriminant analysis to analyze the relationship between the studied populations and to identify the variables that show the highest multiple correlations with these populations. For molecular characterization, DNA was extracted and purified from five random white females from each population. The internal transcribed spacer (ITS1) regions were subjected to direct sequencing to study the diversity of these populations. Discriminant analysis of the morphometric traits indicated that the studied populations belong to one species (H....
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Hodgkin's disease 0) represents one of the most common human lymphoma entities associated with production of various cytokines and, growth factors. Cytokine receptors implying a predominant role for cytokines in the pathophysiology of... more
Hodgkin's disease 0) represents one of the most common human lymphoma entities associated with production of various cytokines and, growth factors. Cytokine receptors implying a predominant role for cytokines in the pathophysiology of HD as a tumor of cytokine-producing cells. So, studying of granulocyte- macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) may lead to major advances in understanding the molecular
... N. Al-Khalifah King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia 123 ... 4. Total carbohydrates: Total carbohydrates were esti-mated by the difference in the mean values, ie, 100— (sum of concentrations of protein,... more
... N. Al-Khalifah King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia 123 ... 4. Total carbohydrates: Total carbohydrates were esti-mated by the difference in the mean values, ie, 100— (sum of concentrations of protein, ash and lipid). J Am Oil Chem Soc 123 ...
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... by project number DSR-AR-2(52) under the program of the National Strategy Projects, Academy of Scientific Research, King Saud University. ... Nagaraju J, Kathirvel M, Kumar R, Siddiq EA, Hasnain SE (2002) Genetic analysis of... more
... by project number DSR-AR-2(52) under the program of the National Strategy Projects, Academy of Scientific Research, King Saud University. ... Nagaraju J, Kathirvel M, Kumar R, Siddiq EA, Hasnain SE (2002) Genetic analysis of traditional and evolved Basmati and non-Basmati ...
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Go to AGRIS search. Journal of food, agriculture & environment (2004). Molecular characterization of some turfgrass cultivars using randomly amplified polymorphic DNA (RAPD) markers. ...
High temperature is a major environmental stress that devastatingly affects wheat production. Thenceforth, developing heat-tolerant and high-yielding wheat genotypes has become more critical to sustaining wheat production particularly... more
High temperature is a major environmental stress that devastatingly affects wheat production. Thenceforth, developing heat-tolerant and high-yielding wheat genotypes has become more critical to sustaining wheat production particularly under abrupt climate change and fast-growing global population. The present study aimed to evaluate parental genotypes and their cross combinations under normal and heat stress conditions, exploring their diversity based on dehydration-responsive element-binding 2 gene (DREB, stress tolerance gene in response to abiotic stress) in parental genotypes, and determining gene action controlling yield traits through half-diallel analysis. Six diverse bread wheat genotypes (local and exotic) and their 15 F1 hybrids were evaluated at two different locations under timely and late sowing dates. Sowing date, location, genotype, and their interactions significantly impacted the studied traits; days to heading, chlorophyll content, plant height, grain yield, and it...
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Wheat cultivar 'Yeocra Rojo' and ten selected genotypes originating from immature embryo culture of this cultivar were evaluated for their productivity in two field experiments during 2005/2006 and 2006/2007 winter seasons. Wheat... more
Wheat cultivar 'Yeocra Rojo' and ten selected genotypes originating from immature embryo culture of this cultivar were evaluated for their productivity in two field experiments during 2005/2006 and 2006/2007 winter seasons. Wheat genotypes YR-9 and YR-10 exhibited the tallest plants whereas the shortest plants were those of cv. 'Yeocra Rojo'. In general, genotypes YR-2, YR-7, and YR-10 produced the highest grain yield while YR-8 produced the lowest. YR-8 also gave the lowest harvest index. YR-7 had the most grains per spike, spike length and number of spikelets per spike compared to its parent cultivar and all other genotypes. A set of PCR-based markers for specific HMW glutenin genes encoding By-subunits were used to identify wheat genotypes carrying By genes at the Glu-B1 locus for its bread-making quality. The presence of the gene encoding By8, which exists in the allele combination Glu-B1b (Bx7 + By8), was detected only in one genotype: YR-7. Primer pair ZSBy9aF1/R3 gave characteristic banding patterns for Glu-B1c (Bx7+By9) and can therefore be used to discriminate By9-containing alleles from non-By9 alleles. Primer pair ZSBy9F2/R2 produced individuals with a diagnostic banding pattern for allele Glu-B1f (Bx13+By16) in genotype YR-10 while YR-8 and YR-9 did not produce any PCR product that was found to be specific for By-null or the 20 gene. YR-7 and YR-10 produced the highest grain yield and had the By8 and By16 genes, respectively, which are associated with superior bread-making quality. YR-9, however, produced high grain yield but had the By-null or 20 gene, which is associated with poor bread-making quality. Therefore, fast and accurate identification of By genes by molecular markers at the Glu-B1 locus could be an efficient way for early selection of useful wheat genotypes with good bread-making quality.