International Journal of Scientific & Technology Research, 2015
Pathogenic fungi are responsible for high infectious morbidity and mortality in immunodeficient p... more Pathogenic fungi are responsible for high infectious morbidity and mortality in immunodeficient patients, a rapid and accurate identification of pathogenic fungi is critical for appropriate treatment. Recently, many molecular methods have been developed for diagnosis to improve the identification of pathogenic fungi. In this review we compared the advantage and disadvantage of five molecular methods that are widely used in the diagnosis of pathogenic fungi. Keyword: pathogenic, fungi, diagnosis, molecular methods ————————————————————
Birds’ truffles; Picoa spp. have been the topic of few reviews, they are considered between deser... more Birds’ truffles; Picoa spp. have been the topic of few reviews, they are considered between desert truffles which are a valuable food and used as medicine in Arabian Gulf countries including Saudi Arabia and several other countries of the world. Recently, many researchers have demonstrated that desert truffles including bird’s truffles are source of important nutritional elements. Also, present biological activities such as antibacterial and antioxidant activities, have a kind of medicinal properties and used in folk medicine. Meanwhile, birds’ truffles were not investigated for its “cultivation”, ecology, taxonomy, physiology, edibility, medicinal properties as well as antimicrobial activities. Rare studies of bird truffles, could be found in literatures, concerning geographic distribution and molecular studies which were achieved to elucidate the ambiguity of the classification of bird’s truffles; this with the help of the advanced recent techniques in molecular biology. In this p...
For centuries, traditional Chinese medicine has relied on medicinal fungi as a panacea for streng... more For centuries, traditional Chinese medicine has relied on medicinal fungi as a panacea for strengthening the immune system and revitalizing the human body based on the belief that fungi have diverse biological functions. Modernly, Cordyceps militaris has also been a source of several bioactive components in pharmacology and medicine, such as cordycepin, ergosterol and polysaccharide. Cordyceps militaris has been the topic of numerous reviews; however, many of them have not focused on the bioactive compound and medicinal value of this fungus. In this mini-review, we compile recent data on, the latest molecular research, its bioactive compounds and medicinal value.
Cordyceps militaris is one of the well described mushrooms and has been extensively used in Tradi... more Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with specific amount of malt extract (ME) together w...
Zika virus disease is a vector borne disease transmitted by several Aedes species. This virus is ... more Zika virus disease is a vector borne disease transmitted by several Aedes species. This virus is now considered as emerging pathogen. zika virus infection symptoms are generally mild and can be misdiagnosed with many arboviral infections which cause fever and rash . So far, there is no vaccine or treatment available to treat patient with Zika virus infection. Furthermore, numerous studies have strongly suggested that there is an association between zika virus infection and neurological complications like Guillain Barré syndrome. In this mini-review we compile recent data on ZIKA virus and its transmission inside and outside the African continent. Keyword: Zika virus, arbovirus, arboviral infection, vector borne disease
Background: Aedes aegypti is the main vector of the yellow fever and dengue virus. This mosquito ... more Background: Aedes aegypti is the main vector of the yellow fever and dengue virus. This mosquito has become the major indirect cause of morbidity and mortality of the human worldwide. Dengue virus activity has been reported recently in the western areas of Saudi Arabia. There is no vaccine for dengue virus until now, and the control of the disease depends on the control of the vector. Objectives: The present study has aimed to perform phylogenetic analysis of Aedes aegypti based on mitochondrial NADH dehydrogenase subunit 4 (ND4) gene at Almadinah, Saudi Arabia in order to get further insight into the epidemiology and transmission of this vector. Materials and Methods: Mitochondrial ND4 gene was sequenced in the eight isolated Aedes aegypti mosquitoes from Almadinah, Saudi Arabia, sequences were aligned, and phylogenetic analysis were performed and compared with 54 sequences of Aedes reported in the previous studies from Mexico, Thailand, Brazil, and Africa. Results: Our results suggest that increased gene flow among Aedes aegypti populations occurs between Africa and Saudi Arabia. Conclusions: Phylogenetic relationship analysis showed two genetically distinct Aedes aegypti in Saudi Arabia derived from dual African ancestor.
For centuries, traditional Chinese medicine has relied on medicinal fungi as a panacea for streng... more For centuries, traditional Chinese medicine has relied on medicinal fungi as a panacea for strengthening the immune system and revitalizing the human body based on the belief that fungi have diverse biological functions. Modernly, Cordyceps militaris has also been a source of several bioactive components in pharmacology and medicine, such as cordycepin, ergosterol and polysaccharide. Cordyceps militaris has been the topic of numerous reviews; however, many of them have not focused on the bioactive compound and medicinal value of this fungus. In this mini-review, we compile recent data on, the latest molecular research, its bioactive compounds and medicinal value.
Birds' truffles; Picoa spp. have been subject to few studies. Birds' truffles are considered amon... more Birds' truffles; Picoa spp. have been subject to few studies. Birds' truffles are considered among desert truffles and a valuable food. Further, they were used as medicine in Arabian Gulf countries including Saudi Arabia and several other countries of the world. Recently, many researchers have demonstrated that desert truffles including bird's truffles are source of important nutritional elements. In addition, they have some biological activities such as antibacterial, antioxidant activities, kind of medicinal properties and used in folk medicine. Meanwhile, birds' truffles were not investigated for its cultivation, ecology, taxonomy, physiology, edibility, medicinal properties as well as antimicrobial activities. Rare studies on birds' truffles could be found in surveyed literatures concerning geographic distribution and molecular studies. These molecular research were achieved to elucidate the ambiguity of the classification of bird's truffles; this with the help of the advanced recent techniques in molecular biology. Hence, in this present article, we compile recent data on the importance of Picoa spp. as desert truffles fungi. The Picoa can play a significant role in biological control agents and provide food for birds. Moreover, the decomposition of fruit-bodies of Picoa in the soils can improve the physical, chemical and biological conditions of the soils that will improve their ecological conditions.
Zika virus disease is a vector borne disease transmitted by several Aedes species. This virus is ... more Zika virus disease is a vector borne disease transmitted by several Aedes species. This virus is now considered as emerging pathogen. zika virus infection symptoms are generally mild and can be misdiagnosed with many arboviral infections which cause fever and rash. So far, there is no vaccine or treatment available to treat patient with Zika virus infection. Furthermore, numerous studies have strongly suggested that there is an association between zika virus infection and neurological complications like Guillain-Barré syndrome. In this mini-review we compile recent data on ZIKA virus and its transmission inside and outside the African continent.
Cordyceps militaris is one of the well described mushrooms and has been extensively used in
Tradi... more Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth diameter was monitored during 21 days of cultivation using two series of experiments of different medium supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA medium supplemented with 6 g/L ME and 4 g/L. YE.
Abstract: Pathogenic fungi are responsible for high infectious morbidity and mortality in immuno... more Abstract: Pathogenic fungi are responsible for high infectious morbidity and mortality in immunodeficient patients, a rapid and accurate identification of pathogenic fungi is critical for appropriate treatment. Recently, many molecular methods have been developed for diagnosis to improve the identification of pathogenic fungi. In this review we compared the advantage and disadvantage of five molecular methods that are widely used in the diagnosis of pathogenic fungi.
Cordyceps fungi have attracted many mycologists since their discovery because of their peculiar c... more Cordyceps fungi have attracted many mycologists since their discovery because of their peculiar characteristics of fruiting on their host invertebrate animals. Cordyceps fungi, which parasitize insects and change into fruiting bodies, are rich sources of biologically active compounds that are implicated for improvement in health and for prevention and treatment of various diseases. They are considered to be some of the more promising medicinal mushrooms. Cordyceps sinensis has been noted for its potential for medicinal use and hence extensive pharmaceutical studies have been made. In this review I compile available data on the characteristics of Cordyceps sinensis that appear to be effective as a natural source of bioactive compounds.
Keywords - bioactive compounds, Cordyceps sinensis, caterpillar fungus, medicinal mushrooms.
International Journal of Scientific & Technology Research, 2015
Pathogenic fungi are responsible for high infectious morbidity and mortality in immunodeficient p... more Pathogenic fungi are responsible for high infectious morbidity and mortality in immunodeficient patients, a rapid and accurate identification of pathogenic fungi is critical for appropriate treatment. Recently, many molecular methods have been developed for diagnosis to improve the identification of pathogenic fungi. In this review we compared the advantage and disadvantage of five molecular methods that are widely used in the diagnosis of pathogenic fungi. Keyword: pathogenic, fungi, diagnosis, molecular methods ————————————————————
Birds’ truffles; Picoa spp. have been the topic of few reviews, they are considered between deser... more Birds’ truffles; Picoa spp. have been the topic of few reviews, they are considered between desert truffles which are a valuable food and used as medicine in Arabian Gulf countries including Saudi Arabia and several other countries of the world. Recently, many researchers have demonstrated that desert truffles including bird’s truffles are source of important nutritional elements. Also, present biological activities such as antibacterial and antioxidant activities, have a kind of medicinal properties and used in folk medicine. Meanwhile, birds’ truffles were not investigated for its “cultivation”, ecology, taxonomy, physiology, edibility, medicinal properties as well as antimicrobial activities. Rare studies of bird truffles, could be found in literatures, concerning geographic distribution and molecular studies which were achieved to elucidate the ambiguity of the classification of bird’s truffles; this with the help of the advanced recent techniques in molecular biology. In this p...
For centuries, traditional Chinese medicine has relied on medicinal fungi as a panacea for streng... more For centuries, traditional Chinese medicine has relied on medicinal fungi as a panacea for strengthening the immune system and revitalizing the human body based on the belief that fungi have diverse biological functions. Modernly, Cordyceps militaris has also been a source of several bioactive components in pharmacology and medicine, such as cordycepin, ergosterol and polysaccharide. Cordyceps militaris has been the topic of numerous reviews; however, many of them have not focused on the bioactive compound and medicinal value of this fungus. In this mini-review, we compile recent data on, the latest molecular research, its bioactive compounds and medicinal value.
Cordyceps militaris is one of the well described mushrooms and has been extensively used in Tradi... more Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with specific amount of malt extract (ME) together w...
Zika virus disease is a vector borne disease transmitted by several Aedes species. This virus is ... more Zika virus disease is a vector borne disease transmitted by several Aedes species. This virus is now considered as emerging pathogen. zika virus infection symptoms are generally mild and can be misdiagnosed with many arboviral infections which cause fever and rash . So far, there is no vaccine or treatment available to treat patient with Zika virus infection. Furthermore, numerous studies have strongly suggested that there is an association between zika virus infection and neurological complications like Guillain Barré syndrome. In this mini-review we compile recent data on ZIKA virus and its transmission inside and outside the African continent. Keyword: Zika virus, arbovirus, arboviral infection, vector borne disease
Background: Aedes aegypti is the main vector of the yellow fever and dengue virus. This mosquito ... more Background: Aedes aegypti is the main vector of the yellow fever and dengue virus. This mosquito has become the major indirect cause of morbidity and mortality of the human worldwide. Dengue virus activity has been reported recently in the western areas of Saudi Arabia. There is no vaccine for dengue virus until now, and the control of the disease depends on the control of the vector. Objectives: The present study has aimed to perform phylogenetic analysis of Aedes aegypti based on mitochondrial NADH dehydrogenase subunit 4 (ND4) gene at Almadinah, Saudi Arabia in order to get further insight into the epidemiology and transmission of this vector. Materials and Methods: Mitochondrial ND4 gene was sequenced in the eight isolated Aedes aegypti mosquitoes from Almadinah, Saudi Arabia, sequences were aligned, and phylogenetic analysis were performed and compared with 54 sequences of Aedes reported in the previous studies from Mexico, Thailand, Brazil, and Africa. Results: Our results suggest that increased gene flow among Aedes aegypti populations occurs between Africa and Saudi Arabia. Conclusions: Phylogenetic relationship analysis showed two genetically distinct Aedes aegypti in Saudi Arabia derived from dual African ancestor.
For centuries, traditional Chinese medicine has relied on medicinal fungi as a panacea for streng... more For centuries, traditional Chinese medicine has relied on medicinal fungi as a panacea for strengthening the immune system and revitalizing the human body based on the belief that fungi have diverse biological functions. Modernly, Cordyceps militaris has also been a source of several bioactive components in pharmacology and medicine, such as cordycepin, ergosterol and polysaccharide. Cordyceps militaris has been the topic of numerous reviews; however, many of them have not focused on the bioactive compound and medicinal value of this fungus. In this mini-review, we compile recent data on, the latest molecular research, its bioactive compounds and medicinal value.
Birds' truffles; Picoa spp. have been subject to few studies. Birds' truffles are considered amon... more Birds' truffles; Picoa spp. have been subject to few studies. Birds' truffles are considered among desert truffles and a valuable food. Further, they were used as medicine in Arabian Gulf countries including Saudi Arabia and several other countries of the world. Recently, many researchers have demonstrated that desert truffles including bird's truffles are source of important nutritional elements. In addition, they have some biological activities such as antibacterial, antioxidant activities, kind of medicinal properties and used in folk medicine. Meanwhile, birds' truffles were not investigated for its cultivation, ecology, taxonomy, physiology, edibility, medicinal properties as well as antimicrobial activities. Rare studies on birds' truffles could be found in surveyed literatures concerning geographic distribution and molecular studies. These molecular research were achieved to elucidate the ambiguity of the classification of bird's truffles; this with the help of the advanced recent techniques in molecular biology. Hence, in this present article, we compile recent data on the importance of Picoa spp. as desert truffles fungi. The Picoa can play a significant role in biological control agents and provide food for birds. Moreover, the decomposition of fruit-bodies of Picoa in the soils can improve the physical, chemical and biological conditions of the soils that will improve their ecological conditions.
Zika virus disease is a vector borne disease transmitted by several Aedes species. This virus is ... more Zika virus disease is a vector borne disease transmitted by several Aedes species. This virus is now considered as emerging pathogen. zika virus infection symptoms are generally mild and can be misdiagnosed with many arboviral infections which cause fever and rash. So far, there is no vaccine or treatment available to treat patient with Zika virus infection. Furthermore, numerous studies have strongly suggested that there is an association between zika virus infection and neurological complications like Guillain-Barré syndrome. In this mini-review we compile recent data on ZIKA virus and its transmission inside and outside the African continent.
Cordyceps militaris is one of the well described mushrooms and has been extensively used in
Tradi... more Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth diameter was monitored during 21 days of cultivation using two series of experiments of different medium supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA medium supplemented with 6 g/L ME and 4 g/L. YE.
Abstract: Pathogenic fungi are responsible for high infectious morbidity and mortality in immuno... more Abstract: Pathogenic fungi are responsible for high infectious morbidity and mortality in immunodeficient patients, a rapid and accurate identification of pathogenic fungi is critical for appropriate treatment. Recently, many molecular methods have been developed for diagnosis to improve the identification of pathogenic fungi. In this review we compared the advantage and disadvantage of five molecular methods that are widely used in the diagnosis of pathogenic fungi.
Cordyceps fungi have attracted many mycologists since their discovery because of their peculiar c... more Cordyceps fungi have attracted many mycologists since their discovery because of their peculiar characteristics of fruiting on their host invertebrate animals. Cordyceps fungi, which parasitize insects and change into fruiting bodies, are rich sources of biologically active compounds that are implicated for improvement in health and for prevention and treatment of various diseases. They are considered to be some of the more promising medicinal mushrooms. Cordyceps sinensis has been noted for its potential for medicinal use and hence extensive pharmaceutical studies have been made. In this review I compile available data on the characteristics of Cordyceps sinensis that appear to be effective as a natural source of bioactive compounds.
Keywords - bioactive compounds, Cordyceps sinensis, caterpillar fungus, medicinal mushrooms.
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Papers by Mouhanad AL ALI
Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or
in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and
polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most
potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This
mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation
during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom
on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation
medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with
specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth
diameter was monitored during 21 days of cultivation using two series of experiments of different medium
supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained
results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA
medium supplemented with 6 g/L ME and 4 g/L. YE.
pathogenic fungi is critical for appropriate treatment. Recently, many molecular methods have been developed for diagnosis to improve the identification
of pathogenic fungi. In this review we compared the advantage and disadvantage of five molecular methods that are widely used in the diagnosis of
pathogenic fungi.
Keywords - bioactive compounds, Cordyceps sinensis, caterpillar fungus, medicinal mushrooms.
Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or
in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and
polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most
potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This
mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation
during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom
on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation
medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with
specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth
diameter was monitored during 21 days of cultivation using two series of experiments of different medium
supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained
results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA
medium supplemented with 6 g/L ME and 4 g/L. YE.
pathogenic fungi is critical for appropriate treatment. Recently, many molecular methods have been developed for diagnosis to improve the identification
of pathogenic fungi. In this review we compared the advantage and disadvantage of five molecular methods that are widely used in the diagnosis of
pathogenic fungi.
Keywords - bioactive compounds, Cordyceps sinensis, caterpillar fungus, medicinal mushrooms.