Books by Justyna Nowakowska
Precise identification of biological samples remains the most important proof in the forensic sci... more Precise identification of biological samples remains the most important proof in the forensic science. Illegal logging has become the urgent issue in Poland during the last decades, and conventional methods of investigation turn out to be often insufficient. Recently, the DNA-based markers (SSR and cytoplasmic genes) can remarkably help in the forensic botany performed by the Forest Service Guards and the Police investigation in illegal logging of timber. The identification method relies on comparison of the piece of evidence (i.e., stolen wood fragments) with the piece of reference (e.g., tree parts remained in the forest). We present the usefulness of the DNA neutral markers (i.e., microsatellite loci) and cytoplasmic genes in forensic botany based on several case studies of illegal wood identification in Poland, concerning the most economically important coniferous tree species such as Pinus sylvestris L., Picea abies (L.) Karst., Abies alba Mill., and Larix decidua (L.). Thanks to the DNA profiles established on the basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmic organelle (mitochondrial or chlo-roplast) DNA marker, the determination of the DNA profiles provided fast and reliable comparison between material of evidence (also wood and needles) and material of reference (first of all tree stumps) in the forest. These data strongly supported the decision taken by several District Courts in Poland, as far as the
Title: Latitudinal variability of the radiation microclimate in artificial forest gaps in Poland ... more Title: Latitudinal variability of the radiation microclimate in artificial forest gaps in Poland – the modelling perspective, Author(s): Bolibok L., Brach M., Drozdowski S., Szeligowski H., DOI: 10.1515/frp-2016-0001; Title: Effects of food source quality on the adults of Melolontha melolontha and M. hippocastani, Author(s): Woreta D., Lipiński S., Wolski R., DOI: 10.1515/frp-2016-0002; Title: The vulnerability of silver fir populations to damage from late frosts, Author(s): Klisz M., Jastrzębowski S., Ukalska J., Przybylski P., Matras J., Mionskowski M., DOI: 10.1515/frp-2016-0003; Title: The dynamics of northern red oak (Quercus rubra L.) in managed forests of central Poland, Author(s): Głowacki D., Sławska M., Sławski M., DOI: 10.1515/frp-2016-0004; Title: Density of red squirrels and their use of non-native tree species in the Rogów Arboretum, Author(s): Krauze-Gryz D., Mazur K., Gryz J., DOI: 10.1515/frp-2016-0005; Title: Economic methods for the utilisation of logging residues, Author(s): Gałęzia T., DOI: 10.1515/frp-2016-0006; Title: The change in weight and surface temperature of pine cones (Pinus sylvestris L.) as a result of microwave irradiation, Author(s): Aniszewska M., DOI: 10.1515/frp-2016-0007; Title: Preliminary studies on the molecular identification of sex in Taxus baccata L., Author(s): Zarek M., DOI: 10.1515/frp-2016-0008; Title: The use of phosphates in forestry, Author(s): Tkaczyk M., Kubiak K.A., Sawicki J., Nowakowska J.A., Oszako T., DOI: 10.1515/frp-2016-0009
Forest Research Papers, Vol. 75 (1), 2014
Title: Environmental conditions that promote the occur... more Forest Research Papers, Vol. 75 (1), 2014
Title: Environmental conditions that promote the occurrence of truffles (Tuber spp.) on historical sites in Poland
Author(s): Rosa-Gruszecka A., Hilszczańska D., Szmidla H.
DOI: 10.2478/frp-2014-0001
Title: A comparison of lower montane natural forest (Abies, Fagus, Picea) in Oszast Reserve and spruce monocultures in the Żywiecki Beskid and Śląski Beskid
Author(s): Jaworski A., Pach M.
DOI: 10.2478/frp-2014-0002
Title: Analysis of the duration of basic logging operations performed using a chainsaw
Author(s): Gałęzia T.
DOI: 10.2478/frp-2014-0003
Title: The influence of different thinning methods on dominance coefficients of future crop trees in even-aged Scots pine stands
Author(s): Zachara T.
DOI: 10.2478/frp-2014-0004
Title: Genetic variability of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst.) natural regeneration compared with their maternal stands
Author(s): Nowakowska J. A., Zachara T., Konecka A.
DOI: 10.2478/frp-2014-0005
Title: Evaluating variation in logged timber costs and raw timber prices during the period, 2001–2009, for the Regional Directorate of the State Forests in Zielona Góra
Author(s): Adamowicz K., Kaciunka H.
DOI: 10.2478/frp-2014-0006
Title: Natural regeneration in the ‘Czmoń' nature reserve (Wielkopolska Region)
Author(s): Horodecki P., Wiczyńska K., Jagodziński A. M.
DOI: 10.2478/frp-2014-0007
Title: Vegetation diversity of the Scots pine stands in different forest sites in the Turawa Forest District
Author(s): Stefańska-Krzaczek E., Pech P.
DOI: 10.2478/frp-2014-0008
Title: Opportunity costs of establishing nature reserves in selected forest districts of the Mazowieckie Province
Author(s): Kaliszewski A., Młynarski W.
DOI: 10.2478/frp-2014-0009
Title: Transformation of methane in peatlands environments
Author(s): Stępniewska Z., Goraj W., Kuźniar A.
DOI: 10.2478/frp-2014-0010
Folia Forestalia Polonica 2015, Vol. 57 (3)
Title: Basic soil properties as a factor controllin... more Folia Forestalia Polonica 2015, Vol. 57 (3)
Title: Basic soil properties as a factor controlling the occurrence and intensity of water repellency in rankers of the White Carpathians
Author(s): Lucia Kořenková, Martin Urík
DOI: 10.1515/ffp-2015-0013
Title: Preliminary analysis of the forest health state based on multispectral images acquired
by Unmanned Aerial Vehicle
Author(s): Paweł Czapski, Mariusz Kacprzak, Jan Kotlarz, Karol Mrowiec, Katarzyna Kubiak, Miłosz Tkaczyk
DOI: 10.1515/ffp-2015-0014
Title: Litterfall and growth dynamics relationship with the meteorological variability in three forests in the Montseny natural park
Author(s): Jordi Bou, Antònia Caritat, Lluís Vilar
DOI: 10.1515/ffp-2015-0015
Title: Encroachment of woody species on a drained transitional peat bog in ‘Mszar Bogdaniec’ nature reserve (Western Poland)
Author(s): Marcin K. Dyderski, Anna K. Gdula, Andrzej M. Jagodziński
DOI: 10.1515/ffp-2015-0016
Title: Stakeholder analysis in the biomass energy development based on the experts’ opinions: the example of Triglav National Park in Slovenia
Author(s): Gianluca Grilli, Giulia Garegnani, Aleš Poljanec, Andrej Ficko, Daniele Vettorato, Isabella De Meo, Alessandro Paletto
DOI: 10.1515/ffp-2015-0017
Title: Effect of winter conditions on wild ungulates mortality in the Owl Mountains (Poland)
Author(s): Marcin Warchałowski, Piotr Nowakowski, Andrzej Dancewicz
DOI: 10.1515/ffp-2015-0018
Title: Climate change and food security: challenges for plant health, plant breeding and genetic resources
Author(s): Tomasz Oszako, Justyna A. Nowakowska
DOI: 10.1515/ffp-2015-0019
Papers by Justyna Nowakowska
phosphite preparations are now an important alternative in plant protection against new, invasive... more phosphite preparations are now an important alternative in plant protection against new, invasive pathogens of the genus Phytophthora and/or Pythium. It is crucial to intervene when alien, invasive oomycetes are carried to plantations or forest stands and attack fine roots via zoospores. the aim of this paper was to demonstrate the possibility of phosphite application to induce resistance to tree pathogens. phosphate-based fertilizers have been used successfully in nurseries, where application is relatively easy by means of foliar sprays. the traditional fungicides, which are effective in combating fungi, however, fail to control oomycetes. Instead, they mask the disease, which in turn causes serious damage to seedlings after they have been planted in a suitable environment. Moreover, the number of effective fungicides available for forest plant protection has continued to decrease in the last decade. the effectiveness of the chemicals is reduced due to their frequent use and their similarity in terms of the active compound or the mechanism of action. given the low diversity of active compounds, it is necessary to monitor the development of resistance of pathogens to fungicides by means of molecular biology (sequencing and quantitative pCr). Minimizing the undesired side effects of chemicals on both, mycorrhizal fungi and pathogens can be achieved by strict adherence to rigorous security measures and, where possible, frequently changing the active compounds to alternatives such as phosphites. the significance of phosphate and phosphite uptake by trees is still a matter of debate, especially under field conditions. Nevertheless, phosphites are environmentally friendly compounds, which constitute an alternative or complement to the traditional chemicals (in accordance with the directive on Integrated plant management).
AbstrAct Fungi colonising oak seedlings in forest plantations in northeastern Poland were identif... more AbstrAct Fungi colonising oak seedlings in forest plantations in northeastern Poland were identified in 2014–2015. The evaluated 4-to 6-year bare root oaks originated from the Olsztynek Forest District (Regional Directorate of the State Forests in Olsztyn). In total, 744 fungal isolates belonging to 11 different species, and 11 genera, were identified in tested plants. Amongst them, 186 cultures (25%) were classified as plant pathogens. The most commonly isolated pathogenic fungi belonged to the Fusarium and Cylindrocarpon genera.
The possibility of using chemicals in European forestry is extremely limited due to the binding l... more The possibility of using chemicals in European forestry is extremely limited due to the binding legal regulations and specific conditions concerning the market of plant protection products. This is reflected in the limited availability of active fungicides in forestry. Due to this limitation, practitioners using fungicides in forest nurseries and forest cultivation must have substantial knowledge of the biology of pathogens to ensure satisfactorily effective protection. The work presented here provides an overview of the currently recommended fungicides in Polish forestry as well as the mechanisms of interaction between the active substances and the pathogen, the plant and mycorrhizal fungi. The risk of fungicide resistance, which has been insufficiently explored in the context of forest pathogens, is also discussed in this paper.
Szybka diagnostyka czynników patogenicznych w szkółkach leśnych jest dziś możliwa do przeprowadze... more Szybka diagnostyka czynników patogenicznych w szkółkach leśnych jest dziś możliwa do przeprowadzenia na podstawie
analiz DNA.
Concn. of PHO 3 2– ions in plant tissues was detd. by using reaction with Ag + in acidic soln. to... more Concn. of PHO 3 2– ions in plant tissues was detd. by using reaction with Ag + in acidic soln. to metallic Ag ppt. The amt. of Ag was detd. by X-ray fluorescence spectrometry (XRF) and UV-VIS spectrophotometry. The XRF method was less laborious, but its accuracy was lower. Its sensitivity was insufficient to det. very low concns. of phosphonates. The UV-VIS spectrophotometry, after Ag dissoln. in concd. HNO 3 , complexation of Ag + with dithizone, extn. with CHCl 3 and detn. without removal of free dithizone, showed a significantly higher sensitivity. Opracowano metodę ilościowego oznaczania fosfonianów w tkankach roślinnych. Zaproponowana procedura oparta jest na reakcji tych jonów z jonami srebra w środowisku kwaśnym, w wyniku której strąca się osad metaliczne-go srebra. Ilość wolnego srebra, powstałego w wyniku działania fosfonianów, została oznaczona metodami spektrometrii fluorescencji rentgenowskiej XRF i spektrofotometrii UV-VIS. Metoda XRF jest mniej pracochłonna, lecz jej czułość to ok. 0,1 mg srebra, co nie stanowi wyniku zadowalającego w przypadku śladowych ilości fosfonianów w tkankach roślinnych. W przypadku spektrofotometrii UV-VIS, po roztworzeniu srebra w stężonym HNO 3 , skompleksowaniu jonów Ag + ditizonem, ekstrakcji chloroformem i bezpośrednim oznaczeniu spektrofotometrycznym bez usuwania
Oszako T. 2016. Molekularna diagnostyka wybranych patogenów z rodzaju Phytophthora w ramach integ... more Oszako T. 2016. Molekularna diagnostyka wybranych patogenów z rodzaju Phytophthora w ramach integrowanej ochrony roślin. Sylwan 160 (5): 365−370. Traditional detection methods such as baiting or direct isolation take a long time and are incapable to handling large volume of material to be tested. The real−time PCR−based techniques are faster, more sensitive, more easily automated, and do not require post−amplification procedures. Species−specific primers for Phytophthora were designed based on the internal transcribed spacer regions (ITS) of rDNA collected from the NCBI DNA database. Primers and probes were designed using the Allele ID 7 at default search criteria. Specific probes were labeled with the reporter dyes JOE (6−carboxy−4,5−dichloro−2,7−dimethoxyfluorescein) at the 5' end and HBQ1 quencher at the 3' end (Sigma−Aldrich). The specificity of primers and fluorogenic probes was tested against genomic DNA of P. alni subsp. multiformis, P. lacustris and P. taxon hungarica. The real−time PCR reactions with the specific probes and primers yielded positive results with five concentrations of standards obtained by standard PCR reaction for corresponding Phytophthora species. The negative control (lack of DNA pathogens) yielded no amplification products. Standard curves showed a linear correlation between input DNA and cycle threshold (Ct) values with R 2 from 0.994 (P. alni) to 0.998 (P. taxon hungarica). The amplification efficiency of target DNA varied from 94.6% (P. alni) to 100% (P. taxon hungarica). The validation of the primers and probes designed for analysed Phytophthora species was performed on pure cultures, on soil samples from the forest nursery and declining oak stands. The designed probes displayed the high specificity of the detection of investigated species in pure cultures. The presented new molecular TaqMan probes can fully assist the integrated pest management as a powerful tool for a quick detection of above pathogenic organisms in forest nurseries. The molecular detection of harmful phytoph− thoras and in consequences diminishing of fungicides use for their control in forestry fully support European Union directives as well as the 'Good plant protection practice measures' elaborated by European and Mediterranean Organisation of Plant Protection.
Journal of Forensic Sciences
The paper describes the first case of a Polish court using reliable DNA identification of the har... more The paper describes the first case of a Polish court using reliable DNA identification of the harmful pathogens Heterobasidion annosum (s. str.) and H. parviporum, causing major wood root rot in Norway spruce (Picea abies L. Karst.). In 2011, the District Court in E. received a complaint from a non-governmental organization concerning the illegal removal of spruces growing in a hedge close to private residential buildings. The Court asked the Forest research Institute to confirm the validity of the local authority’s decision to remove the spruce hedge. To this end, two means of diagnosis were applied, i.e. visual estimation of symptoms of root rot disease caused by Heterobasidion spp. and their identification in decayed wood tissues on the basis of molecular DNA methods. Typical rot symptoms of spruce stamps were noticed in two out of ten wood samples and the broad extent of rot in their inner parts was indicative of the disease having developed over a very long time. The genetic st...
Forest Research Papers, 2014
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Books by Justyna Nowakowska
Title: Environmental conditions that promote the occurrence of truffles (Tuber spp.) on historical sites in Poland
Author(s): Rosa-Gruszecka A., Hilszczańska D., Szmidla H.
DOI: 10.2478/frp-2014-0001
Title: A comparison of lower montane natural forest (Abies, Fagus, Picea) in Oszast Reserve and spruce monocultures in the Żywiecki Beskid and Śląski Beskid
Author(s): Jaworski A., Pach M.
DOI: 10.2478/frp-2014-0002
Title: Analysis of the duration of basic logging operations performed using a chainsaw
Author(s): Gałęzia T.
DOI: 10.2478/frp-2014-0003
Title: The influence of different thinning methods on dominance coefficients of future crop trees in even-aged Scots pine stands
Author(s): Zachara T.
DOI: 10.2478/frp-2014-0004
Title: Genetic variability of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst.) natural regeneration compared with their maternal stands
Author(s): Nowakowska J. A., Zachara T., Konecka A.
DOI: 10.2478/frp-2014-0005
Title: Evaluating variation in logged timber costs and raw timber prices during the period, 2001–2009, for the Regional Directorate of the State Forests in Zielona Góra
Author(s): Adamowicz K., Kaciunka H.
DOI: 10.2478/frp-2014-0006
Title: Natural regeneration in the ‘Czmoń' nature reserve (Wielkopolska Region)
Author(s): Horodecki P., Wiczyńska K., Jagodziński A. M.
DOI: 10.2478/frp-2014-0007
Title: Vegetation diversity of the Scots pine stands in different forest sites in the Turawa Forest District
Author(s): Stefańska-Krzaczek E., Pech P.
DOI: 10.2478/frp-2014-0008
Title: Opportunity costs of establishing nature reserves in selected forest districts of the Mazowieckie Province
Author(s): Kaliszewski A., Młynarski W.
DOI: 10.2478/frp-2014-0009
Title: Transformation of methane in peatlands environments
Author(s): Stępniewska Z., Goraj W., Kuźniar A.
DOI: 10.2478/frp-2014-0010
Title: Basic soil properties as a factor controlling the occurrence and intensity of water repellency in rankers of the White Carpathians
Author(s): Lucia Kořenková, Martin Urík
DOI: 10.1515/ffp-2015-0013
Title: Preliminary analysis of the forest health state based on multispectral images acquired
by Unmanned Aerial Vehicle
Author(s): Paweł Czapski, Mariusz Kacprzak, Jan Kotlarz, Karol Mrowiec, Katarzyna Kubiak, Miłosz Tkaczyk
DOI: 10.1515/ffp-2015-0014
Title: Litterfall and growth dynamics relationship with the meteorological variability in three forests in the Montseny natural park
Author(s): Jordi Bou, Antònia Caritat, Lluís Vilar
DOI: 10.1515/ffp-2015-0015
Title: Encroachment of woody species on a drained transitional peat bog in ‘Mszar Bogdaniec’ nature reserve (Western Poland)
Author(s): Marcin K. Dyderski, Anna K. Gdula, Andrzej M. Jagodziński
DOI: 10.1515/ffp-2015-0016
Title: Stakeholder analysis in the biomass energy development based on the experts’ opinions: the example of Triglav National Park in Slovenia
Author(s): Gianluca Grilli, Giulia Garegnani, Aleš Poljanec, Andrej Ficko, Daniele Vettorato, Isabella De Meo, Alessandro Paletto
DOI: 10.1515/ffp-2015-0017
Title: Effect of winter conditions on wild ungulates mortality in the Owl Mountains (Poland)
Author(s): Marcin Warchałowski, Piotr Nowakowski, Andrzej Dancewicz
DOI: 10.1515/ffp-2015-0018
Title: Climate change and food security: challenges for plant health, plant breeding and genetic resources
Author(s): Tomasz Oszako, Justyna A. Nowakowska
DOI: 10.1515/ffp-2015-0019
Papers by Justyna Nowakowska
analiz DNA.
Title: Environmental conditions that promote the occurrence of truffles (Tuber spp.) on historical sites in Poland
Author(s): Rosa-Gruszecka A., Hilszczańska D., Szmidla H.
DOI: 10.2478/frp-2014-0001
Title: A comparison of lower montane natural forest (Abies, Fagus, Picea) in Oszast Reserve and spruce monocultures in the Żywiecki Beskid and Śląski Beskid
Author(s): Jaworski A., Pach M.
DOI: 10.2478/frp-2014-0002
Title: Analysis of the duration of basic logging operations performed using a chainsaw
Author(s): Gałęzia T.
DOI: 10.2478/frp-2014-0003
Title: The influence of different thinning methods on dominance coefficients of future crop trees in even-aged Scots pine stands
Author(s): Zachara T.
DOI: 10.2478/frp-2014-0004
Title: Genetic variability of Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst.) natural regeneration compared with their maternal stands
Author(s): Nowakowska J. A., Zachara T., Konecka A.
DOI: 10.2478/frp-2014-0005
Title: Evaluating variation in logged timber costs and raw timber prices during the period, 2001–2009, for the Regional Directorate of the State Forests in Zielona Góra
Author(s): Adamowicz K., Kaciunka H.
DOI: 10.2478/frp-2014-0006
Title: Natural regeneration in the ‘Czmoń' nature reserve (Wielkopolska Region)
Author(s): Horodecki P., Wiczyńska K., Jagodziński A. M.
DOI: 10.2478/frp-2014-0007
Title: Vegetation diversity of the Scots pine stands in different forest sites in the Turawa Forest District
Author(s): Stefańska-Krzaczek E., Pech P.
DOI: 10.2478/frp-2014-0008
Title: Opportunity costs of establishing nature reserves in selected forest districts of the Mazowieckie Province
Author(s): Kaliszewski A., Młynarski W.
DOI: 10.2478/frp-2014-0009
Title: Transformation of methane in peatlands environments
Author(s): Stępniewska Z., Goraj W., Kuźniar A.
DOI: 10.2478/frp-2014-0010
Title: Basic soil properties as a factor controlling the occurrence and intensity of water repellency in rankers of the White Carpathians
Author(s): Lucia Kořenková, Martin Urík
DOI: 10.1515/ffp-2015-0013
Title: Preliminary analysis of the forest health state based on multispectral images acquired
by Unmanned Aerial Vehicle
Author(s): Paweł Czapski, Mariusz Kacprzak, Jan Kotlarz, Karol Mrowiec, Katarzyna Kubiak, Miłosz Tkaczyk
DOI: 10.1515/ffp-2015-0014
Title: Litterfall and growth dynamics relationship with the meteorological variability in three forests in the Montseny natural park
Author(s): Jordi Bou, Antònia Caritat, Lluís Vilar
DOI: 10.1515/ffp-2015-0015
Title: Encroachment of woody species on a drained transitional peat bog in ‘Mszar Bogdaniec’ nature reserve (Western Poland)
Author(s): Marcin K. Dyderski, Anna K. Gdula, Andrzej M. Jagodziński
DOI: 10.1515/ffp-2015-0016
Title: Stakeholder analysis in the biomass energy development based on the experts’ opinions: the example of Triglav National Park in Slovenia
Author(s): Gianluca Grilli, Giulia Garegnani, Aleš Poljanec, Andrej Ficko, Daniele Vettorato, Isabella De Meo, Alessandro Paletto
DOI: 10.1515/ffp-2015-0017
Title: Effect of winter conditions on wild ungulates mortality in the Owl Mountains (Poland)
Author(s): Marcin Warchałowski, Piotr Nowakowski, Andrzej Dancewicz
DOI: 10.1515/ffp-2015-0018
Title: Climate change and food security: challenges for plant health, plant breeding and genetic resources
Author(s): Tomasz Oszako, Justyna A. Nowakowska
DOI: 10.1515/ffp-2015-0019
analiz DNA.