Turkiye Klinikleri Journal of Medical Sciences, 2010
Objective: Cyclin D1 is a key regulatory protein of cell cycle and this oncogenic activity had be... more Objective: Cyclin D1 is a key regulatory protein of cell cycle and this oncogenic activity had been observed in a variety of tumors. P21 is a cyclin dependent kinase inhibitor responsible for cell cycle arrest as a tumor suppressor gene. The studies in the literature about effects ...
Kawasaki syndrome, also known as mucocutaneous lymph node syndrome, is an acute vasculitis of inf... more Kawasaki syndrome, also known as mucocutaneous lymph node syndrome, is an acute vasculitis of infants and young children. We describe a four-year-old girl who presented with fever, a diffuse erythematous maculopapular rash, bilateral nonpurulent bulbar conjunctivitis, dry, red, fissured lips, a tongue with a strawberry "appearance", an erythematous pharynx, indurative erythema, and edema and desquamation of the face, hands and feet. She probably developed mitral valve prolapse during the course of the disease. The diagnosis of Kawasaki syndrome was arrived at by excluding other diseases and by the presence of all the clinical criteria for Kawasaki syndrome. Since this syndrome is rarely encountered in Turkey, this case is presented and the literature regarding the syndrome is reviewed.
We evaluated the antiemetic efficacy of tropisetron for control of acute emesis during grade 3 or... more We evaluated the antiemetic efficacy of tropisetron for control of acute emesis during grade 3 or 4 emetogenic chemotherapy in children. Tropisetron was administered as a single intravenous dose of 0.2 mg/kg on the first day and intravenously or orally with the same dose on subsequent days. A total of 125 courses of highly emetogenic chemotherapy was administered to 22 children with a median age of 14 years (range: 3-18 years). All 22 patients received tropisetron for at least two courses. Overall complete response on day 1 was observed in 80 out of 125 courses (64%). The response rates were consistent over multiple courses; a complete and major response rate on the first day of Course I (n: 22 courses) and Course II (n: 22 courses) was observed in 73 and 77 percent of cases, respectively. When the results were analyzed according to the daily schedules, overall complete response for grade 4, grade 3 and grade 1-2 emetogenic treatment days was 59, 85 and 75 percent, respectively. In ...
The New Microbiologica: official journal of the Italian Society for Medical Virology (SIVIM)
Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) are among the most "successful" ... more Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) are among the most "successful" pathogens and code for a variety of proteins to direct the apoptosis/necrosis responses of the cells they infect. Nitric oxide (NO) is an important intracellular signaling molecule in pathological processes. Acyclovir (ACV) is a chain terminator that targets the viral DNA polymerase as an antiviral agent. In this study, NO signals, and apoptosis/necrosis responses of HEp-2 cells were compared when infected by HSV-1 and -2 for 24 hours against non toxic doses (starting from 48.8, 24.4, 12.2, 6.1, 3 to 1.5 microg/mL) of ACV. In 48.8, 24.4 and 12.2 microg/mL of ACV, HSV-1 had an "upregulating effect" whereas HSV-2 had a "downregulating effect" on NO production, and in 6.1, 3 and 1.5 microg/mL of ACV HSV-1 had a "down-regulating effect" whereas HSV-2 had an "upregulating effect" on NO responses (HSV-1 had a "downregulating effect" on NO produ...
Objective: Organ microenvironment factors such as nitric oxide (NO) and reactive oxygen species (... more Objective: Organ microenvironment factors such as nitric oxide (NO) and reactive oxygen species (ROS), and their interactions with apoptosis may influence the behavior of normal and cancer cells. This possibility may provide new targets for therapy strategies. The effect of NO on tumor cell survival is influenced by multiple factors, including the levels of redox homeostasis, and genetic and epigenetic make up of tumor cells. The effects of ROS [mimicked by hydrogen peroxide(H2O2)] on NO-mediated cellular death were examined in this study. Method: Human cervical carcinoma cell line (HeLa) was grown using Dulbecco's Minimum Essential Medium (DMEM). Cell survival and proliferations were determined by a quantitative colorimetric MTT assay. The DNA binding dye Hoechst 33342 and propidium iodide (HOE/PI) were used together in a differential dye uptake assay for microscopic identification and quantification of membrane integrity and nuclear morphology. Results: When HeLa cells were i...
Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) are among the most &am... more Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) are among the most "successful" pathogens and code for a variety of proteins to direct the apoptosis/necrosis responses of the cells they infect. Nitric oxide (NO) is an important intracellular signaling molecule in pathological processes. Acyclovir (ACV) is a chain terminator that targets the viral DNA polymerase as an antiviral agent. In this study, NO signals, and apoptosis/necrosis responses of HEp-2 cells were compared when infected by HSV-1 and -2 for 24 hours against non toxic doses (starting from 48.8, 24.4, 12.2, 6.1, 3 to 1.5 microg/mL) of ACV. In 48.8, 24.4 and 12.2 microg/mL of ACV, HSV-1 had an "upregulating effect" whereas HSV-2 had a "downregulating effect" on NO production, and in 6.1, 3 and 1.5 microg/mL of ACV HSV-1 had a "down-regulating effect" whereas HSV-2 had an "upregulating effect" on NO responses (HSV-1 had a "downregulating effect" on NO production whereas HSV-2 had an "upregulating effect" on NO production without any ACV). In 48.8, 24.4 and 12.2 microg/mL of ACV, HSV-1 had an "anti-apoptotic effect" whereas HSV-2 had a stimulation on "apoptotic effect", and in 6.1, 3 and 1.5 microg/mL of ACV HSV-1 had an "apoptotic effect" and HSV-2 turned to "its natural viral apoptotic effect level" (HSV-1 had an "natural viral apoptotic effect" whereas HSV-2 had a "natural viral apoptotic effect" on apoptosis response without any ACV). In 48.8, and 24.4 microg/mL of ACV, HSV-1 had significant "necrotic effect" on necrotic cellular death, "necrosis" increased in 12.2, 6.1, 3 and 1.5 microg/mL of ACV (HSV-1 had a negligible "necrotic effect" on HEp-2 cells alone), and HSV-2 had a "natural viral necrotic effect" alone; and also in all non toxic ACV concentrations. These results showed that HSV-1 and -2 had different…
Objective: To analyze febrile neutropenic episodes (FNEs) of children with lymphoma and malignant... more Objective: To analyze febrile neutropenic episodes (FNEs) of children with lymphoma and malignant solid tumors (MSTs). Methods: Medical records of children with cancer who had FNEs between July 01, 2005-August 01, 2007 were analyzed. Neutropenia was defined as an absolute neutrophil count (ANC) of <500 cells/mm3 or a count of <1000 cells/ mm3, with a predicted decrease to <500 cells/ mm3 within 24-48 h. Fever was defined as a single axillary temperature ≥38.5°C, or ≥38°C for 1 h, or ≥38°C in two consecutive measurements with at least 4h interval. Results: Seventy-three febrile neutropenic episodes in 33 children were analyzed. The median age of diagnosis was 6 years (5 months-18years), M:F ratio was 2. The diagnosis was lymphoma in 24%, malignant solid tumors (MSTs) in 76%, and 77% of FNEs occurred in patients with MSTs. Patients received prophylactic G-CSF before 78% of the episodes. Clinical and microbiological documentation was available in 55% and 16% of FNEs, respectiv...
Notch expression is not yet determined in hepatoblastoma. In this study the effect of chemotherap... more Notch expression is not yet determined in hepatoblastoma. In this study the effect of chemotherapeutics (cisplatin, doxorubicin, cytosin arabinoside); differentiating agent (13 cis-retinoic acid) and apoptotic agents (5-aza-2'-deoxycytidine, arsenic trioxide) on notch expression in hepatoblastoma were evaluated. After HepG2 cell line was cultured and the agents and their combinations were applied for 24 hour in pre-optimized 50% lethal doses, RNA isolation and cDNA converting, expression of 84 custom array genes of notch signaling pathway (SABiosciences, PAT059F-24) was determined by Real Time PCR. The methylation status of 6 genes that showed more than 5 fold changes compared with control group were explored by Methylation qPCR Assay. High expressed genes are HDAC1, NFKB1, CHUK, CDKN1A, and CBL. Low expressed genes are DLL1, CD44, FZD2, GLI1, IL17B, LMO2, NOTCH1, LOR, PAX5, PT-CRA, SH2D1A, and WISP1. The genes searched for methylation (DLL1, HEY1, DTX1, HDAC1, NOTCH2 and JAG1) ...
We evaluated the incidence of congenital malformations in 566 children (median age: 8, M:F 1.3) w... more We evaluated the incidence of congenital malformations in 566 children (median age: 8, M:F 1.3) with lymphomas and solid tumors using patient records. In this study, 12.7% of children either had a congenital malformation (7.8%) or a birthmark (4.9%). The incidence of patients with a childhood cancer syndrome was 3% and these cases developed typical tumors. The rate of consanguineous marriages was 12.6%, and family history of cancer was positive in 31.2%. Median age at cancer diagnosis, gender, maternal age, history of stillbirth and missed abortion, consanguinity of parents, and family history of cancer were not significantly different in cases with and without a congenital malformation. The most frequent cancers were central nervous system tumors and lymphomas. No remarkable association between a particular anomaly and a specific cancer type could be shown. The high incidence of congenital anomalies in this study may stimulate future large cohort studies in our country.
Aneurysmal bone cyst is a rare benign tumor of the bone that can be difficult to distinguish from... more Aneurysmal bone cyst is a rare benign tumor of the bone that can be difficult to distinguish from malignant tumors, especially when it presents in an unusual location. Herein, we report a six-year-old girl with a primary aneurysmal bone cyst in an uncommon location. It originated from the 4th rib and she presented with a huge chest wall mass. Despite the large size of the cyst, the only symptom was persistent cough. She was successfully treated with total excision of tumor without any complication. The patient has been followed up for 56 months without any recurrence.
Juvenile hypertrophy of the breast (JHB) is an uncommon, benign disorder and typically occurs in ... more Juvenile hypertrophy of the breast (JHB) is an uncommon, benign disorder and typically occurs in peri-pubertal females. The etiology ofJHB is uncertain. It may represent an end-organ hypersensitivity of the breast to normal levels of sex steroids. Clinically, it is characterized by rapid enlargement of breasts, either unilateral or bilateral. The definitive diagnosis is made by histopathologic examination. Treatment recommendations include surgery and hormonal therapy, although hormonal manipulation is still controversial in pediatric patients. Here we report a 13-year-old girl with unilateral JHB who did not require surgery or medical treatment.
We report a pediatric case who developed bleomycin-induced hyperpigmentation and hypersensitivity... more We report a pediatric case who developed bleomycin-induced hyperpigmentation and hypersensitivity reactions to both etoposide and vinblastine while receiving chemotherapy for germ cell tumor. Skin hyperpigmentation related to chemotherapeutic agents has been reported only rarely in pediatric patients. This patient developed a characteristic skin hyperpigmentation which was "flagellate" in appearance. Two features of the hyperpigmentation were noteworthy: development at a low cumulative dose of bleomycin and persistence after cessation of chemotherapy. Additive effect of cisplatinum-induced hyperpigmentation was suggested. Although hypersensitivity reactions to etoposide have been previously reported, hypersensitivity reactions to vinblastine are almost unknown. To our knowledge, this is the first report of hypersensitivity reaction to vinblastine in a child in English literature.
Malignant mesothelioma is a very rare tumor in childhood. Presently, treatment of this disease co... more Malignant mesothelioma is a very rare tumor in childhood. Presently, treatment of this disease continues to be frustrating and prognosis remains poor. We here report a pediatric case of malignant pleural mesothelioma who gave a complete response to ICE-VAC chemotherapy regimen and achieved a long-term survival. An eight-year-old girl underwent exploratory thoracotomy and decortication because of a unilateral loculated and multicystic pleural effusion. Histopathological diagnosis was sarcomatoid pleural malignant mesothelioma. After decortication, chemotherapy with ICE (ifosfamide, carboplatin, etoposide) - VAC (vincristine, adriamycin, cyclophosphamide) combination was started. Six courses of chemotherapy resulted in complete clinical and radiological tumor response. She did not receive any further therapy and remains disease-free three years after the first remission. ICE-VAC chemotherapy combination resulted in a complete tumor response and a long-term disease-free survival for th...
ABSTRACT Abstract: Introduction: Neuroblastoma is the most common extracranial solid tumor of chi... more ABSTRACT Abstract: Introduction: Neuroblastoma is the most common extracranial solid tumor of childhood. It originates from the primordial neural crest cells. The cancer stem cell (CSC) are a small population of tumor cells, termed CSCs that have the ability to proliferate and maintain tumor growth. CSCs have two main characteristics: self- renewal and differentiation. Galectin-1 (Gal-1) has important roles in the cell adhesion and growth, apoptosis, immunomodulation, metastasis, inflammation and mRNA splicing. In this study, we aimed to investigate the expression of Gal-1 in the CD133+ stem cells of two neuroblastoma cell lines with different prognostic characteristics in comparison with their CD133- cells. Materials and Method: We cultured neuroblastoma cell lines SH-SY5Y (N-myc negative) and Kelly (N-myc positive) in DMEM and RPMI 1640 mediums and cultivated in an incubator equipped with 5% CO2 at 37°C. We isolated CD133+ neuroblastoma stem cells with magnetic beads. We spreaded the isolated CD133+ and CD133- Kelly and SHSY5Y neuroblastoma cells onto the polylysine-coated slides and stained with Gal-1 immunocytochemically. We counted 50 different 100 cells for each group and evaluated Gal-1 expression as positive (middle and high expression) and negative (weak and no expression). The results were statistically analyzed with the Mann-Whitney U non-parametric test. Results: The control cells had included both of the CD133+ and CD133- cells. When we evaluated Gal-1 expressions in control cells, Kelly control cells showed more Gal-1 expression than SHSY5Y control cells and this higher expression was more obvious in CD133+ Kelly stem cells. Gal-1 expression in CD133- cells (other cells) was very low. When we evaluated Gal-1 expression in CD133+ SHSY5Y stem cells and in CD133- other SHSY5Y cells, we did not determine a significant difference between them.
Thrombocytopenia is a common hemostatic abnormality in the newborn infant. The early diagnosis of... more Thrombocytopenia is a common hemostatic abnormality in the newborn infant. The early diagnosis of thrombocytopenia and the underlying primary pathology process play an important role in reducing the risk of severe complications and mortality. We performed a 2-year prospective study of 643 neonates admitted to our neonatology unit to determine the frequency, predisposing factors, and clinical impact of thrombocytopenia. Thrombocytopenia developed in 18.2% of the preterm neonates and 0.8% of the term neonates. Prematurity, sepsis, hypoxia, intrauterine growth retardation, and disseminated intravascular coagulation were identified as predisposing factors for thrombocytopenia. The incidence of complications and mortality were higher in thrombocytopenic infants. Especially the prognosis was worse in cases who had mucosal hemorrhage, without a relation with the degree of thrombocytopenia. The thrombocytopenia occurred by day 2 in 43% of the infants, and resolved by day 8 in 61%. The platelet count nadir occurred by day 2. Since thrombocytopenic infants are at greater risk for bleeding, and the thrombocytopenia itself may have contributed to the high mortality, predisposing factors such as prematurity, infections, hypoxia must be eliminated by providing better care, giving adequate hygiene of both mother and the baby during the prenatal, natal, and neonatal period.
Turkiye Klinikleri Journal of Medical Sciences, 2010
Objective: Cyclin D1 is a key regulatory protein of cell cycle and this oncogenic activity had be... more Objective: Cyclin D1 is a key regulatory protein of cell cycle and this oncogenic activity had been observed in a variety of tumors. P21 is a cyclin dependent kinase inhibitor responsible for cell cycle arrest as a tumor suppressor gene. The studies in the literature about effects ...
Kawasaki syndrome, also known as mucocutaneous lymph node syndrome, is an acute vasculitis of inf... more Kawasaki syndrome, also known as mucocutaneous lymph node syndrome, is an acute vasculitis of infants and young children. We describe a four-year-old girl who presented with fever, a diffuse erythematous maculopapular rash, bilateral nonpurulent bulbar conjunctivitis, dry, red, fissured lips, a tongue with a strawberry "appearance", an erythematous pharynx, indurative erythema, and edema and desquamation of the face, hands and feet. She probably developed mitral valve prolapse during the course of the disease. The diagnosis of Kawasaki syndrome was arrived at by excluding other diseases and by the presence of all the clinical criteria for Kawasaki syndrome. Since this syndrome is rarely encountered in Turkey, this case is presented and the literature regarding the syndrome is reviewed.
We evaluated the antiemetic efficacy of tropisetron for control of acute emesis during grade 3 or... more We evaluated the antiemetic efficacy of tropisetron for control of acute emesis during grade 3 or 4 emetogenic chemotherapy in children. Tropisetron was administered as a single intravenous dose of 0.2 mg/kg on the first day and intravenously or orally with the same dose on subsequent days. A total of 125 courses of highly emetogenic chemotherapy was administered to 22 children with a median age of 14 years (range: 3-18 years). All 22 patients received tropisetron for at least two courses. Overall complete response on day 1 was observed in 80 out of 125 courses (64%). The response rates were consistent over multiple courses; a complete and major response rate on the first day of Course I (n: 22 courses) and Course II (n: 22 courses) was observed in 73 and 77 percent of cases, respectively. When the results were analyzed according to the daily schedules, overall complete response for grade 4, grade 3 and grade 1-2 emetogenic treatment days was 59, 85 and 75 percent, respectively. In ...
The New Microbiologica: official journal of the Italian Society for Medical Virology (SIVIM)
Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) are among the most "successful" ... more Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) are among the most "successful" pathogens and code for a variety of proteins to direct the apoptosis/necrosis responses of the cells they infect. Nitric oxide (NO) is an important intracellular signaling molecule in pathological processes. Acyclovir (ACV) is a chain terminator that targets the viral DNA polymerase as an antiviral agent. In this study, NO signals, and apoptosis/necrosis responses of HEp-2 cells were compared when infected by HSV-1 and -2 for 24 hours against non toxic doses (starting from 48.8, 24.4, 12.2, 6.1, 3 to 1.5 microg/mL) of ACV. In 48.8, 24.4 and 12.2 microg/mL of ACV, HSV-1 had an "upregulating effect" whereas HSV-2 had a "downregulating effect" on NO production, and in 6.1, 3 and 1.5 microg/mL of ACV HSV-1 had a "down-regulating effect" whereas HSV-2 had an "upregulating effect" on NO responses (HSV-1 had a "downregulating effect" on NO produ...
Objective: Organ microenvironment factors such as nitric oxide (NO) and reactive oxygen species (... more Objective: Organ microenvironment factors such as nitric oxide (NO) and reactive oxygen species (ROS), and their interactions with apoptosis may influence the behavior of normal and cancer cells. This possibility may provide new targets for therapy strategies. The effect of NO on tumor cell survival is influenced by multiple factors, including the levels of redox homeostasis, and genetic and epigenetic make up of tumor cells. The effects of ROS [mimicked by hydrogen peroxide(H2O2)] on NO-mediated cellular death were examined in this study. Method: Human cervical carcinoma cell line (HeLa) was grown using Dulbecco's Minimum Essential Medium (DMEM). Cell survival and proliferations were determined by a quantitative colorimetric MTT assay. The DNA binding dye Hoechst 33342 and propidium iodide (HOE/PI) were used together in a differential dye uptake assay for microscopic identification and quantification of membrane integrity and nuclear morphology. Results: When HeLa cells were i...
Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) are among the most &amp;amp;amp;amp;am... more Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) are among the most &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;successful&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; pathogens and code for a variety of proteins to direct the apoptosis/necrosis responses of the cells they infect. Nitric oxide (NO) is an important intracellular signaling molecule in pathological processes. Acyclovir (ACV) is a chain terminator that targets the viral DNA polymerase as an antiviral agent. In this study, NO signals, and apoptosis/necrosis responses of HEp-2 cells were compared when infected by HSV-1 and -2 for 24 hours against non toxic doses (starting from 48.8, 24.4, 12.2, 6.1, 3 to 1.5 microg/mL) of ACV. In 48.8, 24.4 and 12.2 microg/mL of ACV, HSV-1 had an &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;upregulating effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; whereas HSV-2 had a &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;downregulating effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; on NO production, and in 6.1, 3 and 1.5 microg/mL of ACV HSV-1 had a &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;down-regulating effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; whereas HSV-2 had an &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;upregulating effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; on NO responses (HSV-1 had a &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;downregulating effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; on NO production whereas HSV-2 had an &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;upregulating effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; on NO production without any ACV). In 48.8, 24.4 and 12.2 microg/mL of ACV, HSV-1 had an &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;anti-apoptotic effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; whereas HSV-2 had a stimulation on &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;apoptotic effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;, and in 6.1, 3 and 1.5 microg/mL of ACV HSV-1 had an &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;apoptotic effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; and HSV-2 turned to &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;its natural viral apoptotic effect level&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; (HSV-1 had an &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;natural viral apoptotic effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; whereas HSV-2 had a &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;natural viral apoptotic effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; on apoptosis response without any ACV). In 48.8, and 24.4 microg/mL of ACV, HSV-1 had significant &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;necrotic effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; on necrotic cellular death, &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;necrosis&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; increased in 12.2, 6.1, 3 and 1.5 microg/mL of ACV (HSV-1 had a negligible &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;necrotic effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; on HEp-2 cells alone), and HSV-2 had a &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;natural viral necrotic effect&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot; alone; and also in all non toxic ACV concentrations. These results showed that HSV-1 and -2 had different…
Objective: To analyze febrile neutropenic episodes (FNEs) of children with lymphoma and malignant... more Objective: To analyze febrile neutropenic episodes (FNEs) of children with lymphoma and malignant solid tumors (MSTs). Methods: Medical records of children with cancer who had FNEs between July 01, 2005-August 01, 2007 were analyzed. Neutropenia was defined as an absolute neutrophil count (ANC) of <500 cells/mm3 or a count of <1000 cells/ mm3, with a predicted decrease to <500 cells/ mm3 within 24-48 h. Fever was defined as a single axillary temperature ≥38.5°C, or ≥38°C for 1 h, or ≥38°C in two consecutive measurements with at least 4h interval. Results: Seventy-three febrile neutropenic episodes in 33 children were analyzed. The median age of diagnosis was 6 years (5 months-18years), M:F ratio was 2. The diagnosis was lymphoma in 24%, malignant solid tumors (MSTs) in 76%, and 77% of FNEs occurred in patients with MSTs. Patients received prophylactic G-CSF before 78% of the episodes. Clinical and microbiological documentation was available in 55% and 16% of FNEs, respectiv...
Notch expression is not yet determined in hepatoblastoma. In this study the effect of chemotherap... more Notch expression is not yet determined in hepatoblastoma. In this study the effect of chemotherapeutics (cisplatin, doxorubicin, cytosin arabinoside); differentiating agent (13 cis-retinoic acid) and apoptotic agents (5-aza-2'-deoxycytidine, arsenic trioxide) on notch expression in hepatoblastoma were evaluated. After HepG2 cell line was cultured and the agents and their combinations were applied for 24 hour in pre-optimized 50% lethal doses, RNA isolation and cDNA converting, expression of 84 custom array genes of notch signaling pathway (SABiosciences, PAT059F-24) was determined by Real Time PCR. The methylation status of 6 genes that showed more than 5 fold changes compared with control group were explored by Methylation qPCR Assay. High expressed genes are HDAC1, NFKB1, CHUK, CDKN1A, and CBL. Low expressed genes are DLL1, CD44, FZD2, GLI1, IL17B, LMO2, NOTCH1, LOR, PAX5, PT-CRA, SH2D1A, and WISP1. The genes searched for methylation (DLL1, HEY1, DTX1, HDAC1, NOTCH2 and JAG1) ...
We evaluated the incidence of congenital malformations in 566 children (median age: 8, M:F 1.3) w... more We evaluated the incidence of congenital malformations in 566 children (median age: 8, M:F 1.3) with lymphomas and solid tumors using patient records. In this study, 12.7% of children either had a congenital malformation (7.8%) or a birthmark (4.9%). The incidence of patients with a childhood cancer syndrome was 3% and these cases developed typical tumors. The rate of consanguineous marriages was 12.6%, and family history of cancer was positive in 31.2%. Median age at cancer diagnosis, gender, maternal age, history of stillbirth and missed abortion, consanguinity of parents, and family history of cancer were not significantly different in cases with and without a congenital malformation. The most frequent cancers were central nervous system tumors and lymphomas. No remarkable association between a particular anomaly and a specific cancer type could be shown. The high incidence of congenital anomalies in this study may stimulate future large cohort studies in our country.
Aneurysmal bone cyst is a rare benign tumor of the bone that can be difficult to distinguish from... more Aneurysmal bone cyst is a rare benign tumor of the bone that can be difficult to distinguish from malignant tumors, especially when it presents in an unusual location. Herein, we report a six-year-old girl with a primary aneurysmal bone cyst in an uncommon location. It originated from the 4th rib and she presented with a huge chest wall mass. Despite the large size of the cyst, the only symptom was persistent cough. She was successfully treated with total excision of tumor without any complication. The patient has been followed up for 56 months without any recurrence.
Juvenile hypertrophy of the breast (JHB) is an uncommon, benign disorder and typically occurs in ... more Juvenile hypertrophy of the breast (JHB) is an uncommon, benign disorder and typically occurs in peri-pubertal females. The etiology ofJHB is uncertain. It may represent an end-organ hypersensitivity of the breast to normal levels of sex steroids. Clinically, it is characterized by rapid enlargement of breasts, either unilateral or bilateral. The definitive diagnosis is made by histopathologic examination. Treatment recommendations include surgery and hormonal therapy, although hormonal manipulation is still controversial in pediatric patients. Here we report a 13-year-old girl with unilateral JHB who did not require surgery or medical treatment.
We report a pediatric case who developed bleomycin-induced hyperpigmentation and hypersensitivity... more We report a pediatric case who developed bleomycin-induced hyperpigmentation and hypersensitivity reactions to both etoposide and vinblastine while receiving chemotherapy for germ cell tumor. Skin hyperpigmentation related to chemotherapeutic agents has been reported only rarely in pediatric patients. This patient developed a characteristic skin hyperpigmentation which was "flagellate" in appearance. Two features of the hyperpigmentation were noteworthy: development at a low cumulative dose of bleomycin and persistence after cessation of chemotherapy. Additive effect of cisplatinum-induced hyperpigmentation was suggested. Although hypersensitivity reactions to etoposide have been previously reported, hypersensitivity reactions to vinblastine are almost unknown. To our knowledge, this is the first report of hypersensitivity reaction to vinblastine in a child in English literature.
Malignant mesothelioma is a very rare tumor in childhood. Presently, treatment of this disease co... more Malignant mesothelioma is a very rare tumor in childhood. Presently, treatment of this disease continues to be frustrating and prognosis remains poor. We here report a pediatric case of malignant pleural mesothelioma who gave a complete response to ICE-VAC chemotherapy regimen and achieved a long-term survival. An eight-year-old girl underwent exploratory thoracotomy and decortication because of a unilateral loculated and multicystic pleural effusion. Histopathological diagnosis was sarcomatoid pleural malignant mesothelioma. After decortication, chemotherapy with ICE (ifosfamide, carboplatin, etoposide) - VAC (vincristine, adriamycin, cyclophosphamide) combination was started. Six courses of chemotherapy resulted in complete clinical and radiological tumor response. She did not receive any further therapy and remains disease-free three years after the first remission. ICE-VAC chemotherapy combination resulted in a complete tumor response and a long-term disease-free survival for th...
ABSTRACT Abstract: Introduction: Neuroblastoma is the most common extracranial solid tumor of chi... more ABSTRACT Abstract: Introduction: Neuroblastoma is the most common extracranial solid tumor of childhood. It originates from the primordial neural crest cells. The cancer stem cell (CSC) are a small population of tumor cells, termed CSCs that have the ability to proliferate and maintain tumor growth. CSCs have two main characteristics: self- renewal and differentiation. Galectin-1 (Gal-1) has important roles in the cell adhesion and growth, apoptosis, immunomodulation, metastasis, inflammation and mRNA splicing. In this study, we aimed to investigate the expression of Gal-1 in the CD133+ stem cells of two neuroblastoma cell lines with different prognostic characteristics in comparison with their CD133- cells. Materials and Method: We cultured neuroblastoma cell lines SH-SY5Y (N-myc negative) and Kelly (N-myc positive) in DMEM and RPMI 1640 mediums and cultivated in an incubator equipped with 5% CO2 at 37°C. We isolated CD133+ neuroblastoma stem cells with magnetic beads. We spreaded the isolated CD133+ and CD133- Kelly and SHSY5Y neuroblastoma cells onto the polylysine-coated slides and stained with Gal-1 immunocytochemically. We counted 50 different 100 cells for each group and evaluated Gal-1 expression as positive (middle and high expression) and negative (weak and no expression). The results were statistically analyzed with the Mann-Whitney U non-parametric test. Results: The control cells had included both of the CD133+ and CD133- cells. When we evaluated Gal-1 expressions in control cells, Kelly control cells showed more Gal-1 expression than SHSY5Y control cells and this higher expression was more obvious in CD133+ Kelly stem cells. Gal-1 expression in CD133- cells (other cells) was very low. When we evaluated Gal-1 expression in CD133+ SHSY5Y stem cells and in CD133- other SHSY5Y cells, we did not determine a significant difference between them.
Thrombocytopenia is a common hemostatic abnormality in the newborn infant. The early diagnosis of... more Thrombocytopenia is a common hemostatic abnormality in the newborn infant. The early diagnosis of thrombocytopenia and the underlying primary pathology process play an important role in reducing the risk of severe complications and mortality. We performed a 2-year prospective study of 643 neonates admitted to our neonatology unit to determine the frequency, predisposing factors, and clinical impact of thrombocytopenia. Thrombocytopenia developed in 18.2% of the preterm neonates and 0.8% of the term neonates. Prematurity, sepsis, hypoxia, intrauterine growth retardation, and disseminated intravascular coagulation were identified as predisposing factors for thrombocytopenia. The incidence of complications and mortality were higher in thrombocytopenic infants. Especially the prognosis was worse in cases who had mucosal hemorrhage, without a relation with the degree of thrombocytopenia. The thrombocytopenia occurred by day 2 in 43% of the infants, and resolved by day 8 in 61%. The platelet count nadir occurred by day 2. Since thrombocytopenic infants are at greater risk for bleeding, and the thrombocytopenia itself may have contributed to the high mortality, predisposing factors such as prematurity, infections, hypoxia must be eliminated by providing better care, giving adequate hygiene of both mother and the baby during the prenatal, natal, and neonatal period.
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Papers by Nur Olgun