Visando contribuir para o Plano de Gestão (Manejo) da Floresta Nacional de Ipanema (FLONA), o pre... more Visando contribuir para o Plano de Gestão (Manejo) da Floresta Nacional de Ipanema (FLONA), o presente trabalho objetivou espacializar sua vegetação natural potencial e realizar o mapeamento da vegetação e usos atuais das terras, oferecendo subsídios básicos para seu planejamento ambiental. Para tanto, foi elaborado um croqui, espacializando a vegetação potencial da FLONA, utilizando: as descrições da paisagem de viagens de naturalistas (Saint-Hilaire, Spix e Martius) ao Brasil, no século XIX; estudos mais recentes sobre a vegetação da FLONA e a caracterização edáfica da área apresentada pela Carta de Solos da Fazenda Ipanema (escala 1:10.000). Com base na interpretação de fotos aéreas (escala 1:25.000 Terrafoto, 1972) e verificações de campo, organizou-se o mapa de Vegetação e Usos Atuais das Terras (na escala 1:35.000). Considerando-se os conceitos de clímax climático e clímaces edáficos concluiuse que a vegetação potencial da FLONA seria de Floresta Estacional Semidecidual com ...
Visando contribuir para a gestão da Floresta Nacional de Ipanema,este trabalho apresenta uma abor... more Visando contribuir para a gestão da Floresta Nacional de Ipanema,este trabalho apresenta uma abordagem metodológica que permiteidentificar unidades de paisagem (1:35.000) utilizando a sobreposiçãode cartas temáticas (escalas entre 1:10.000 e 1:50.000). Combase nesses dados foi elaborada uma proposta de zoneamentoque oferece subsídios básicos para o planejamento ambiental.
The aim of this paper was to investigate the effect of chlorogenic acid (5-caffeoylquinic acid, 5... more The aim of this paper was to investigate the effect of chlorogenic acid (5-caffeoylquinic acid, 5CQA), isolated from Baccharis oxyodonta, on the structure and pharmacological effect of secretory phospholipase A2 (sPLA2) from Crotalus durissus terrificus. All in vitro and in vivo experiments were conducted using a purified sPLA2 compared under the same experimental conditions with sPLA2 : 5CQA. 5CQA induced several discrete modifications in the secondary structure and the hydrophobic characteristics of native sPLA2 that induced slight changes in the α-helical content, increase in the random coil structure, and decrease of fluorescence of native sPLA2. Moreover, 5CQA significantly decreased the enzymatic activity and the oedema and myonecrosis induced by native sPLA2. As the catalytic activity of sPLA2 plays an important role in several of its biological and pharmacological properties, antibacterial activity was used to confirm the decrease in its enzymatic activity by 5CQA, which ind...
O desenvolvimento sustentável não tem sido ainda bem entendido porque a sustentabilidade, como de... more O desenvolvimento sustentável não tem sido ainda bem entendido porque a sustentabilidade, como definida pela Comissão Brundtland, constitui uma composição de muitas partes e, como resultado, apresenta ambiciosos objetivos políticos. Este artigo revisa e discute o desenvolvimento sustentável, os impactos socioeconômicos sobre a biodiversidade e as políticas e práticas ambientais no Brasil, com o propósito de promover a conservação da natureza em unidades de conservação. No estudo de caso da Floresta Nacional de Ipanema (Iperó/SP), os resultados mostram que a perda de hábitat e a fragmentação provocam um complexo de mudanças na biodiversidade. Essas mudanças mostram que é uma ilusão infeliz e perigosa imaginar que uma Unidade de Conservação de Uso Sustentável pode promover a conservação dos recursos naturais, da biodiversidade e da integridade do ecossistema. Sustainable Development and Conservation of the Nature in Protected Areas: the case of Ipanema National Forest (Iperó/SP) The s...
Parasitic protozoan diseases affect the poorest population in developing countries. Leishmaniasis... more Parasitic protozoan diseases affect the poorest population in developing countries. Leishmaniasis and Chagas disease have been included among the most important threats for public health in Central and South American continent, with few therapeutic alternatives and highly toxic drugs. In the course of selection of novel drug candidates, we studied the anti-protozoal potential of Drimys brasiliensis. Thus, the crude hexane extract from stem bark as well as its main derivative, the sesquiterpene polygodial, were tested using in vitro assays. The crude hexane extract and polygodial showed activity against Leishmania spp. in the range between 22 and 62 μg/mL, but polygodial demonstrated high parasite selectivity towards Trypanosoma cruzi trypomastigotes (2 μg/mL), with a selectivity index of 19. Finally, polygodial showed a leishmanicidal effect, inducing intense ultrastructural damages in Leishmania in short-time incubation. The obtained results suggested that polygodial could be used as a tool for drug design studies against protozoan diseases and as a candidate molecule for further in vivo studies against T. cruzi.
Species of Baccharis exhibit antibiotic, antiseptic, wound-healing, and anti-protozoal properties... more Species of Baccharis exhibit antibiotic, antiseptic, wound-healing, and anti-protozoal properties, and have been used in the traditional medicine of South America for the treatment of several diseases. In the present work, the fractionation of EtOH extract from aerial parts of Baccharis uncinella indicated that the isolated compounds caffeic acid and pectolinaringenin showed inhibitory activity against Leishmania (L.) amazonensis and Leishmania (V.) braziliensis promastigotes, respectively. Moreover, amastigote forms of both species were highly sensible to the fraction composed by oleanolic + ursolic acids and pectolinaringenin. Caffeic acid also inhibited amastigote forms of L. (L.) amazonensis, but this effect was weak in L. (V.) braziliensis amastigotes. The treatment of infected macrophages with these compounds did not alter the levels of nitrates, indicating a direct effect of the compounds on amastigote stages. The results presented herein suggest that the active components from B. uncinella can be important to the design of new drugs against American tegumentar leishmaniases.
In the course of selection of new bioactive compounds from Brazilian flora, the crude MeOH extrac... more In the course of selection of new bioactive compounds from Brazilian flora, the crude MeOH extract from the leaves of Baccharis retusa DC. (Asteraceae) showed potential against Leishmania sp. and Trypanosoma cruzi. Chromatographic fractionation of the dichloromethane phase from MeOH extract yielded great amounts of the bioactive derivative, which was characterized as 5,6,7-trihydroxy-4'-methoxyflavanone. The structure of this compound was established on the basis of spectroscopic data analysis, mainly nuclear magnetic resonance and mass spectrometry.
The chemical composition and seasonal variation throughout one year of the essential oils from le... more The chemical composition and seasonal variation throughout one year of the essential oils from leaves of Baccharis microdonta and B. elaeagnoides, collected in Campos do Jordão, SP, were investigated. The composition of the latter species has been described for the first time. By GC (RI) and GC/MS analysis, 43 compounds were identified, and a predominance of oxygenated sesquiterpene derivatives was found in both species. The main components of the B. microdonta oils were elemol (31; 11.7-30.6%), spathulenol (34; 4.7-9.1%), β-caryophyllene (19; 3.7-6.2%), and germacrene D (24; 2.9-12.2%), and those of the B. elaeagnoides oils were 34 (10.1-21.5%), viridiflorol (35; 3.6-18.4%), 24 (0.9-13.8%), and 19 (3.5-9.4%). The identified compounds were grouped according to their respective C-skeletons, and the percentages of occurrence of the C-skeletons in the essential oils of leaves collected in the four seasons allowed identifying the preferential accumulation of different types of C-skeletons as well as the seasonal variation of the biosynthetic routes over the studied period.
Visando contribuir para o Plano de Gestão (Manejo) da Floresta Nacional de Ipanema (FLONA), o pre... more Visando contribuir para o Plano de Gestão (Manejo) da Floresta Nacional de Ipanema (FLONA), o presente trabalho objetivou espacializar sua vegetação natural potencial e realizar o mapeamento da vegetação e usos atuais das terras, oferecendo subsídios básicos para seu planejamento ambiental. Para tanto, foi elaborado um croqui, espacializando a vegetação potencial da FLONA, utilizando: as descrições da paisagem de viagens de naturalistas (Saint-Hilaire, Spix e Martius) ao Brasil, no século XIX; estudos mais recentes sobre a vegetação da FLONA e a caracterização edáfica da área apresentada pela Carta de Solos da Fazenda Ipanema (escala 1:10.000). Com base na interpretação de fotos aéreas (escala 1:25.000 Terrafoto, 1972) e verificações de campo, organizou-se o mapa de Vegetação e Usos Atuais das Terras (na escala 1:35.000). Considerando-se os conceitos de clímax climático e clímaces edáficos concluiuse que a vegetação potencial da FLONA seria de Floresta Estacional Semidecidual com ...
Visando contribuir para a gestão da Floresta Nacional de Ipanema,este trabalho apresenta uma abor... more Visando contribuir para a gestão da Floresta Nacional de Ipanema,este trabalho apresenta uma abordagem metodológica que permiteidentificar unidades de paisagem (1:35.000) utilizando a sobreposiçãode cartas temáticas (escalas entre 1:10.000 e 1:50.000). Combase nesses dados foi elaborada uma proposta de zoneamentoque oferece subsídios básicos para o planejamento ambiental.
The aim of this paper was to investigate the effect of chlorogenic acid (5-caffeoylquinic acid, 5... more The aim of this paper was to investigate the effect of chlorogenic acid (5-caffeoylquinic acid, 5CQA), isolated from Baccharis oxyodonta, on the structure and pharmacological effect of secretory phospholipase A2 (sPLA2) from Crotalus durissus terrificus. All in vitro and in vivo experiments were conducted using a purified sPLA2 compared under the same experimental conditions with sPLA2 : 5CQA. 5CQA induced several discrete modifications in the secondary structure and the hydrophobic characteristics of native sPLA2 that induced slight changes in the α-helical content, increase in the random coil structure, and decrease of fluorescence of native sPLA2. Moreover, 5CQA significantly decreased the enzymatic activity and the oedema and myonecrosis induced by native sPLA2. As the catalytic activity of sPLA2 plays an important role in several of its biological and pharmacological properties, antibacterial activity was used to confirm the decrease in its enzymatic activity by 5CQA, which ind...
O desenvolvimento sustentável não tem sido ainda bem entendido porque a sustentabilidade, como de... more O desenvolvimento sustentável não tem sido ainda bem entendido porque a sustentabilidade, como definida pela Comissão Brundtland, constitui uma composição de muitas partes e, como resultado, apresenta ambiciosos objetivos políticos. Este artigo revisa e discute o desenvolvimento sustentável, os impactos socioeconômicos sobre a biodiversidade e as políticas e práticas ambientais no Brasil, com o propósito de promover a conservação da natureza em unidades de conservação. No estudo de caso da Floresta Nacional de Ipanema (Iperó/SP), os resultados mostram que a perda de hábitat e a fragmentação provocam um complexo de mudanças na biodiversidade. Essas mudanças mostram que é uma ilusão infeliz e perigosa imaginar que uma Unidade de Conservação de Uso Sustentável pode promover a conservação dos recursos naturais, da biodiversidade e da integridade do ecossistema. Sustainable Development and Conservation of the Nature in Protected Areas: the case of Ipanema National Forest (Iperó/SP) The s...
Parasitic protozoan diseases affect the poorest population in developing countries. Leishmaniasis... more Parasitic protozoan diseases affect the poorest population in developing countries. Leishmaniasis and Chagas disease have been included among the most important threats for public health in Central and South American continent, with few therapeutic alternatives and highly toxic drugs. In the course of selection of novel drug candidates, we studied the anti-protozoal potential of Drimys brasiliensis. Thus, the crude hexane extract from stem bark as well as its main derivative, the sesquiterpene polygodial, were tested using in vitro assays. The crude hexane extract and polygodial showed activity against Leishmania spp. in the range between 22 and 62 μg/mL, but polygodial demonstrated high parasite selectivity towards Trypanosoma cruzi trypomastigotes (2 μg/mL), with a selectivity index of 19. Finally, polygodial showed a leishmanicidal effect, inducing intense ultrastructural damages in Leishmania in short-time incubation. The obtained results suggested that polygodial could be used as a tool for drug design studies against protozoan diseases and as a candidate molecule for further in vivo studies against T. cruzi.
Species of Baccharis exhibit antibiotic, antiseptic, wound-healing, and anti-protozoal properties... more Species of Baccharis exhibit antibiotic, antiseptic, wound-healing, and anti-protozoal properties, and have been used in the traditional medicine of South America for the treatment of several diseases. In the present work, the fractionation of EtOH extract from aerial parts of Baccharis uncinella indicated that the isolated compounds caffeic acid and pectolinaringenin showed inhibitory activity against Leishmania (L.) amazonensis and Leishmania (V.) braziliensis promastigotes, respectively. Moreover, amastigote forms of both species were highly sensible to the fraction composed by oleanolic + ursolic acids and pectolinaringenin. Caffeic acid also inhibited amastigote forms of L. (L.) amazonensis, but this effect was weak in L. (V.) braziliensis amastigotes. The treatment of infected macrophages with these compounds did not alter the levels of nitrates, indicating a direct effect of the compounds on amastigote stages. The results presented herein suggest that the active components from B. uncinella can be important to the design of new drugs against American tegumentar leishmaniases.
In the course of selection of new bioactive compounds from Brazilian flora, the crude MeOH extrac... more In the course of selection of new bioactive compounds from Brazilian flora, the crude MeOH extract from the leaves of Baccharis retusa DC. (Asteraceae) showed potential against Leishmania sp. and Trypanosoma cruzi. Chromatographic fractionation of the dichloromethane phase from MeOH extract yielded great amounts of the bioactive derivative, which was characterized as 5,6,7-trihydroxy-4'-methoxyflavanone. The structure of this compound was established on the basis of spectroscopic data analysis, mainly nuclear magnetic resonance and mass spectrometry.
The chemical composition and seasonal variation throughout one year of the essential oils from le... more The chemical composition and seasonal variation throughout one year of the essential oils from leaves of Baccharis microdonta and B. elaeagnoides, collected in Campos do Jordão, SP, were investigated. The composition of the latter species has been described for the first time. By GC (RI) and GC/MS analysis, 43 compounds were identified, and a predominance of oxygenated sesquiterpene derivatives was found in both species. The main components of the B. microdonta oils were elemol (31; 11.7-30.6%), spathulenol (34; 4.7-9.1%), β-caryophyllene (19; 3.7-6.2%), and germacrene D (24; 2.9-12.2%), and those of the B. elaeagnoides oils were 34 (10.1-21.5%), viridiflorol (35; 3.6-18.4%), 24 (0.9-13.8%), and 19 (3.5-9.4%). The identified compounds were grouped according to their respective C-skeletons, and the percentages of occurrence of the C-skeletons in the essential oils of leaves collected in the four seasons allowed identifying the preferential accumulation of different types of C-skeletons as well as the seasonal variation of the biosynthetic routes over the studied period.
Uploads