- Transgenics, Plant Tissue Culture and Genetic Transformation, Plant Biotechnology and Molecular Biology, Plant Stress Physiology, Transgenic plants, Plant Biology, and 6 morePlant Molecular Biology, Stress physiology and molecular biology of plants, Molecular plant pathology, Plant microbe interactions, Plant transgenics, and Plant biotechnologyedit
The evolutionary origin of fungi is important in determining the phylogenetic relationships between fungi, animals and plants. However, determining the true relationship of fungi has been somewhat difficult owing to their simple... more
The evolutionary origin of fungi is important in determining the phylogenetic relationships between fungi, animals and plants. However, determining the true relationship of fungi has been somewhat difficult owing to their simple morphology and presence of convergent characters. With the advent of newer molecular techniques, analysis of conserved protein sequences, cytochrome systems, mitochondrial and nuclear genetic material and rRNAs are being employed in elucidating phylogenetic kinships among the eukaryotes. These emerging evidences suggest that the derivation of the fungi from plants or algae would require more evolutionary changes than its derivation from protozoa, the unicellular ancestor of the animals. To gain an overview of the current state of the science, we have extensively reviewed the existing literatures and it appeared to us that fungi are more closely related to animals than to plants.
The involvement of glutathione (GSH) in plant defense against pathogen invasion is an established fact. However, the molecular mechanism conferring this tolerance remains to be explored. Here, proteomic analysis of pad2-1, an Arabidopsis... more
The involvement of glutathione (GSH) in plant defense against pathogen invasion is an established fact. However, the molecular mechanism conferring this tolerance remains to be explored. Here, proteomic analysis of pad2-1, an Arabidopsis thaliana GSH-depleted mutant, in response to Pseudomonas syringae infection has been performed to explore the intricate position of GSH in defense against biotrophic pathogens. The pad2-1 mutant displayed severe susceptibility to P. syringae infection compared to the wild-type (Col-0) thus re-establishing a fundamental role of GSH in defense. Apart from general up-accumulation of energy metabolism-related protein-species in both infected Col-0 and pad2-1, several crucial defense-related protein-species were identified to be differentially accumulated. Leucine-rich repeat-receptor kinase (LRR-RK) and nucleotide-binding site-leucine-rich repeat resistance protein (NBS-LRR), known to play a pioneering role against pathogen attack, were only weakly up-accumulated in pad2-1 after infection. Transcriptional and post-transcriptional regulators like MYB-P1 and glycine-rich repeat RNA-binding protein (GRP) and several other stress-related protein-species like heat shock protein 17 (HSP17) and glutathione-S-transferase (GST) were also identified to be differentially regulated in pad2-1 and Col-0 in response to infection. Together, the present investigation reveals that the optimum GSH-level is essential for the efficient activation of plant defense signaling cascades thus conferring resistance to pathogen invasion.
Research Interests:
Research Interests:
The contribution of glutathione (GSH) in stress tolerance, defense response and antioxidant signaling is an established fact. In this study transcriptome analysis of pad2.1, an Arabidopsis thaliana mutant, after combined osmotic and cold... more
The contribution of glutathione (GSH) in stress tolerance, defense response and antioxidant signaling is an established fact. In this study transcriptome analysis of pad2.1, an Arabidopsis thaliana mutant, after combined osmotic and cold stress treatment has been performed to explore the intricate position of GSH in the stress and defense signaling network in planta. Microarray data revealed the differential regulation of about 1674 genes in pad2.1 amongst which 973 and 701 were significantly up- and down-regulated respectively. Gene enrichment, functional pathway analysis by DAVID and MapMan analysis identified various stress and defense related genes viz. members of heat shock protein family, peptidyl prolyl isomerase (PPIase), thioredoxin peroxidase (TPX2), glutathione-S-transferase (GST), NBS-LRR type resistance protein etc. as down-regulated. The expression pattern of the above mentioned stress and defense related genes and APETALA were also validated by comparative proteomic a...
Research Interests:
Research Interests: Pharmacology, Pharmacy, Complementary and Alternative Medicine, Chinese Medicine, Plant Biology, and 37 moreEthnopharmacology, Oxidative Stress, Apoptosis, Liver diseases, Biological Sciences, Antioxidants, Phytotherapy, Liver, Mice, Animals, Male, Glutathione, Ethanol, Catalase, Glutathione Peroxidase, Anti-inflammatory agents, Superoxide Dismutase, CHEMICAL SCIENCES, Antioxidant Activity, Antioxidant enzyme, Ascorbic Acid, Plant extracts, Rats, Hepatocytes, Tumor necrosis factor-alpha, Transforming Growth Factor Beta, Phyllanthus, Rhizome, Glutathione Transferase, Curcuma, Cell Proliferation, Thioacetamide, Drug Induced Liver Injury, Thiobarbituric Acid Reactive Substance, Images of Norml and Damage Liver Cells, Malondialdehyde, and Control Group
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Several signaling molecules critically regulate the physiological responses in plants. Among them, miRNAs, generally 21-24 nucleotides long are widely distributed in different plant species and play as key signaling intermediates in... more
Several signaling molecules critically regulate the physiological responses in plants. Among them, miRNAs, generally 21-24 nucleotides long are widely distributed in different plant species and play as key signaling intermediates in diverse physiological responses. The mature miRNAs are synthesized from MIR genes by RNA polymerase II and processed by Dicer-like (DCL) protein family members associated with some accessory protein molecules. The processed miRNAs are transported to the cytoplasm from the nucleus by specific group of transporters and incorporated into RNA-induced silencing complex (RISC) complex for specific mRNA cleavage. MicroRNAs can suppress the diverse gene expression, depending on the sequence complementarity of the target transcript except of its own gene. Besides it, miRNAs can modulate the gene expression by DNA methylation and translational inhibition of the target transcript. Different classes of DCLs and Argonaute proteins (AGOs) help the miRNAs-mediated gene silencing mechanism in plants.