Schistosomiasis (SCH) is the second only to malaria among the parasitic diseases affecting humans regarding the prevalence of infection worldwide. In this nonsystematic review, we summarize the existing data on commercially available and... more
Schistosomiasis (SCH) is the second only to malaria among the parasitic diseases affecting humans regarding the prevalence of infection worldwide. In this nonsystematic review, we summarize the existing data on commercially available and promising investigational urine markers for the detection of SCH and its associated bladder cancer (BC). We searched PubMed, Scopus and Cochran without time limits. We reviewed the recent literatures on urine-based markers for SCH and its associated BC. Many studies identified several urine biomarkers of Schistosoma haematobium and Schistosoma mansoni worms and their associated BC using automated, inexpensive, quantitative assays in urine. These markers may aid in early detection of bladder carcinoma and have the potential to reduce the number of follow-up cystoscopy, thus reducing healthcare costs and patient discomfort, at the same time. Nevertheless, clinical evidence is insufficient to warrant the substitution of the cystoscopic follow-up scheme by any of the currently available urine marker tests.
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Nanostructured materials such as metallic oxides, possibly iron (II, III) oxide can play an important role in the fields of life sciences such as biomedicine, agriculture, and environment science. Iron (II, III) Oxide nanoparticles... more
Nanostructured materials such as metallic oxides, possibly iron (II, III) oxide can play an important role in the fields of life sciences such as biomedicine, agriculture, and environment science. Iron (II, III) Oxide nanoparticles (FeII,IIINPs) an innovative material in recent nanotechnology due to their unique properties, such as like high surface area to volume ratio, bettered solubility, low toxicity, surface tailoring power, Superparamagnetism or magnetic properties, greater surface area, easy separation methodology, biocompatibility and multiple use making them strong candidate for biomedicine. Currently the interest of using FeII, IIINPs such as magnetic nanoparticles (iron oxide), metallic oxides nanoparticles, nanoshells, and nanocages have been continuously used and modified to enable their use as a diagnostic and therapeutic agent. This review summarizes and initiates the chemistry of Iron (II, III) Oxide nanoparticles related with the methods for the preparation of iron ...
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Abstract A new label-free electrochemical biosensor was progressed for direct detection of unamplified HCV nucleic acid based on silver/zinc bimetallic metal–organic framework (Ag/Zn-MOF). Several physico-chemical techniques were used to... more
Abstract A new label-free electrochemical biosensor was progressed for direct detection of unamplified HCV nucleic acid based on silver/zinc bimetallic metal–organic framework (Ag/Zn-MOF). Several physico-chemical techniques were used to confirm the structure and morphology. The prepared bimetallic-MOF was cast on the glassy carbon electrode (GCE) and treated with the capture probe of HCV. Then, treated with bovine serum albumin (BSA) to passivate the surface, which is ready for hybridization process once the HCV target nucleic acid was added. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used as an evidence for the successful stepwise formation of the desired biosensor. In addition, a differential pulse voltammetry (DPV) measurement was used in the presence of glucose as an electrochemical catalytic indicator. The results confirmed that the bimetallic MOF's catalytic activity stimulated glucose oxidation to gluconolactone, resulting in electrochemical signal amplification. The proposed HCV electrochemical biosensor has several advantages ease of use; low cost; high selectivity and sensitivity; a linear detection ranges from 1 fM to 100 nM; and ultra-low detection limit (0.64 fM). To date, this is the first electrochemical HCV-biosensor report based on bimetallic-MOF. Consequently, this assay opens the door for designing more electrochemical biosensors regarding the electrical and catalytic activity of bimetallic-MOF.
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Hepatocellular carcinoma (HCC) is a noteworthy health problem with a poor diagnosis due to limited detection techniques. Transcriptome studies can be used to classify cancer further away from anatomical location and histopathology. Recent... more
Hepatocellular carcinoma (HCC) is a noteworthy health problem with a poor diagnosis due to limited detection techniques. Transcriptome studies can be used to classify cancer further away from anatomical location and histopathology. Recent studies demonstrated the novelty of numerous types of specific RNA biomarkers that differentially expressed both the normal liver and the HCC tissues, but those specific types overlapped with the detection of other types of cancers. In this study, total RNA was used to ensure the existence of differences between different cancer types. A multispectral light source (340-1000 nm) interacted with the sample. Multi-wavelengths images were captured using a hyperspectral camera (wavelength 380-1000 nm). The optimum wavelength to discriminate between the normal and HCC samples was selected by calculating the optical properties (transmission, absorption and scattered light). Results showed specific spectral signatures for total RNA within the red-band (633...
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A novel label-free electrochemical biosensor constructed using a polyaniline@nickel metal–organic framework (Ni-MOF) nanocomposite for direct detection of HCV-RNA.
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Development of ultra-sensitive, high specific and cost-effective nucleic acids (NAs) biosensors is critical for early diagnosis of cancer, genetic diseases and follows up response to treatment. Metal-organic frameworks (MOFs) as sensing... more
Development of ultra-sensitive, high specific and cost-effective nucleic acids (NAs) biosensors is critical for early diagnosis of cancer, genetic diseases and follows up response to treatment. Metal-organic frameworks (MOFs) as sensing materials underwent significant development in recent years due to their unique merits, such as structural diversity, tunable pore scale, large surface area, remarkable adsorption affinities, and good thermal stability. MOFs have shown potential contribution in nucleic acids biosensors research. Herein, a comprehensive overview on NAs biosensors state of the art based on MOFs has been discussed extensively, including different MOFs platforms sensing strategies (fluorescence, electrochemistry, electrochemiluminescence, and colorimetric techniques), their analytical performance and figures of merit in clinical diagnostics, with the future perspective in introducing MOFs in clinical laboratory diagnostics. Moreover, the different MOFs synthesis methods have been highlighted to serve as a guide for the researchers in selecting the appropriate platform that suits their research needs, and applications.
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The affordable and reliable detection of Hepatitis C Virus (HCV) RNA is a cornerstone in the management and control of infection, affecting approximately 3% of the global population. However, the existing technologies are expensive, labor... more
The affordable and reliable detection of Hepatitis C Virus (HCV) RNA is a cornerstone in the management and control of infection, affecting approximately 3% of the global population. However, the existing technologies are expensive, labor intensive and time consuming, posing significant limitations to their wide-scale exploitation, particularly in economically deprived populations. Here, we utilized the unique optical and physicochemical properties of gold nanoparticles (AuNPs) to develop a novel assay platform shown to be rapid and robust in sensing and quantifying unamplified HCV RNA in clinical samples. The assay is based on inducing aggregation of citrate AuNPs decorated with a specific nucleic acid probe. Two types of cationic AuNPs, cysteamine and CTAB capped, were compared to achieve maximum assay performance. The technology is simple, rapid, cost effective and quantitative with 93.3% sensitivity, high specificity and detection limit of 4.57IU/µl. Finally, our data suggest th...
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Schistosomiasis (SCH) is the second only to malaria among the parasitic diseases affecting humans regarding the prevalence of infection worldwide. In this nonsystematic review, we summarize the existing data on commercially available and... more
Schistosomiasis (SCH) is the second only to malaria among the parasitic diseases affecting humans regarding the prevalence of infection worldwide. In this nonsystematic review, we summarize the existing data on commercially available and promising investigational urine markers for the detection of SCH and its associated bladder cancer (BC). We searched PubMed, Scopus and Cochran without time limits. We reviewed the recent literatures on urine-based markers for SCH and its associated BC. Many studies identified several urine biomarkers of Schistosoma haematobium and Schistosoma mansoni worms and their associated BC using automated, inexpensive, quantitative assays in urine. These markers may aid in early detection of bladder carcinoma and have the potential to reduce the number of follow-up cystoscopy, thus reducing healthcare costs and patient discomfort, at the same time. Nevertheless, clinical evidence is insufficient to warrant the substitution of the cystoscopic follow-up scheme by any of the currently available urine marker tests.
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Research Interests: Biology, Adolescent, Medicine, Hepatitis C, Humans, and 15 moreMutation, Female, Male, Hepatitis C Virus, Clinical Sciences, Middle Aged, Molecular cloning, Genotype, Adult, Base Sequence, Internal ribosome entry site, Antiviral Agents, Molecular Sequence Data, Hepacivirus, and reverse transcriptase polymerase chain reaction
Research Interests: Genetics, Chemistry, Nanoparticle, Nanotechnology, Atomic Force Microscopy, and 15 moreMedicine, Metal Nanoparticles, Adsorption, Antibodies, Graphite, Gold, Gold Nanoparticles, Interface, Molecular sciences, External stimuli, Conjugate, Biological Assay, Biotinylation, Highly Oriented Pyrolytic Graphite, and Nanoconjugates
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The unique properties of metallic nanoparticles have enabled their utilization in biosensing applications. A novel assay for the detection of hepatitis C virus (HCV) RNA in serum specimens has been developed using magnetic nanoparticles... more
The unique properties of metallic nanoparticles have enabled their utilization in biosensing applications. A novel assay for the detection of hepatitis C virus (HCV) RNA in serum specimens has been developed using magnetic nanoparticles and unmodified cationic gold nanoparticles (AuNPs). HCV RNA was extracted using magnetic nanoparticles functionalized with an oligonucleotide specific to HCV RNA. Extracted RNA is reacted with oligonucleotide sequence specific for HCV RNA in presence of unmodified cationic AuNPs. In positive samples, AuNPs are aligned onto the phosphate backbone of the RNA and their aggregation changes the solution color from red to blue. In the absence of target, solution color remains red. The assay has been tested on 50 serum clinical samples (25 HCV positive and 25 controls). The dual nanoparticles assay detected HCV RNA in serum and generated comparable results to real-time PCR. The assay had specificity and a sensitivity of 96% and 96.5%, respectively, and a detection limit of 15 IU/mL. The developed colorimetric dual nanoparticles HCV RNA assay is simple and inexpensive and can be used for rapid detection of unamplified HCV RNA in serum. Similar sensing platforms can be developed to detect other nucleic acid targets.