ABSTRACT In this study, accelerated solvent extraction (ASE) was applied to the quantitative extr... more ABSTRACT In this study, accelerated solvent extraction (ASE) was applied to the quantitative extraction of bixin. The effects of cyclohexane/acetone ratios on bixin extraction yield were evaluated. Acetone was used in the process of pigment extraction and also played a major role in its analysis by UV-Vis spectrophotometry. Pure cis-bixin isolated by flash chromatography and characterized by Fourier Transform Infra Red spectrometry was used as a bixin standard for qualitative and quantitative analysis of annatto extracts which were obtained by accelerated solvents extraction. UV-Vis spectrophotometry analysis shows that the extraction using 100 % cyclohexane gives the lowest bixin yield. This is different from the UV-Vis spectra generated with acetone extraction. The difference in percentage of acetone shows significant effects on bixin extraction yields. The use of cyclohexane:acetone (60 % : 40 %) solution at 50 °C for 5 min heating time results the highest total bixin extraction yield (48.00 %) as compared to the other solvent ratios, and to the usual extraction methods using the same solvent (29.14 %). High pressure liquid chromatography analysis shows that bixin extracted by this method has purity degree of 68.16 %.
... Winda Rahmalia, Fitria Yulistira, Janiar Ningrum, Mahwar Qurbaniah, Muhammad Ismadi Jurusan K... more ... Winda Rahmalia, Fitria Yulistira, Janiar Ningrum, Mahwar Qurbaniah, Muhammad Ismadi Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura, Pontianak ... http://www.hamline.edu/ apakabar/basisdata/1994/11/10/0016.html. 26 Juli 2005. ...
ABSTRACT In this study, accelerated solvent extraction (ASE) was applied to the quantitative extr... more ABSTRACT In this study, accelerated solvent extraction (ASE) was applied to the quantitative extraction of bixin. The effects of cyclohexane/acetone ratios on bixin extraction yield were evaluated. Acetone was used in the process of pigment extraction and also played a major role in its analysis by UV-Vis spectrophotometry. Pure cis-bixin isolated by flash chromatography and characterized by Fourier Transform Infra Red spectrometry was used as a bixin standard for qualitative and quantitative analysis of annatto extracts which were obtained by accelerated solvents extraction. UV-Vis spectrophotometry analysis shows that the extraction using 100 % cyclohexane gives the lowest bixin yield. This is different from the UV-Vis spectra generated with acetone extraction. The difference in percentage of acetone shows significant effects on bixin extraction yields. The use of cyclohexane:acetone (60 % : 40 %) solution at 50 °C for 5 min heating time results the highest total bixin extraction yield (48.00 %) as compared to the other solvent ratios, and to the usual extraction methods using the same solvent (29.14 %). High pressure liquid chromatography analysis shows that bixin extracted by this method has purity degree of 68.16 %.
... Winda Rahmalia, Fitria Yulistira, Janiar Ningrum, Mahwar Qurbaniah, Muhammad Ismadi Jurusan K... more ... Winda Rahmalia, Fitria Yulistira, Janiar Ningrum, Mahwar Qurbaniah, Muhammad Ismadi Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura, Pontianak ... http://www.hamline.edu/ apakabar/basisdata/1994/11/10/0016.html. 26 Juli 2005. ...
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