The use of hydrogen peroxide as a disinfectant material was evaluated for its effect relevant to ... more The use of hydrogen peroxide as a disinfectant material was evaluated for its effect relevant to bacterial contamination which usually occurs in the micropropagation of date palm. Different concentrations of hydrogen peroxide at different time intervals were examined and compared to ethyl alcohol and distilled water with both of callus and shoot explants of date palm cv. Sewi. The most powerful disinfection with 100% survival occurred by using 10 ppm hydrogen peroxide for 1 min.
The Egyptian essential oils derived from the fresh green parts of clove (Syzygium aromaticum), eu... more The Egyptian essential oils derived from the fresh green parts of clove (Syzygium aromaticum), eucalyptus (Cinnamomum eucalyptusa), lemongrass (Cymbopogon citratus), and sweet basil (Osmium basilicum) were evaluated under greenhouse conditions for their oviposition deterrent activity against Rhynchophorus ferrugineus. The highest oviposition deterrent activity was shown by S. aromaticum followed by C. eucalyptusa and cv. citratus oils (at 15% concentration) with values of 98.17%, 97.9% and 94.06 effective repellency, respectively. Cloves and eucalyptus essential oils at concentration of 10 %, significantly reduced egg laying and gave good practical oviposition deterrent effect (80.74 and 66.77% repellency). Moreover, a mixture of S. aromaticum and C. eucalyptusa oils (at 15% concentration) exhibited the higher oviposition deterrent activity (100%) than the other tested oils or their mixtures. These results clearly revealed that both essential oils (S. aromaticum and C. eucalyptusa) can be included as an integral parts of an IPM program against the date palm weevil.
In the present investigation, date palm plant regeneration procedure overcoming four different fu... more In the present investigation, date palm plant regeneration procedure overcoming four different fungus contamination using biological control was described. Three different concentration treatments of actinomycetes culture filtrate (5, 10 and 15%) from two isolates (Streptomyces bobilii and Streptomyces grisiobrunneus) were used to study their effect in overcoming four different fungi which contaminate in vitro date palm i.e. Fusarium moniliforme, Rhizoctonia solani, Macrophomina phaseolina and Alternaria alternata. Medium supplemented with S. bobilii surpassed medium supplemented with S. grisiobrunneus in regeneration characteristic values. Actinomycetes 10 % treatment proved to be the best for enhancing both survived explant and callus induction percentage, while 15% treatment proved to be the best for enhancing regenerated shoots per callus. Herein, we report improved regeneration system for Khalas cultivar, which is strongly needed for successful genetic transformation, overcoming fungi contamination destructiveness.
The use of hydrogen peroxide as a disinfectant material was evaluated for its effect relevant to ... more The use of hydrogen peroxide as a disinfectant material was evaluated for its effect relevant to bacterial contamination which usually occurs in the micropropagation of date palm. Different concentrations of hydrogen peroxide at different time intervals were examined and compared to ethyl alcohol and distilled water with both of callus and shoot explants of date palm cv. Sewi. The most powerful disinfection with 100% survival occurred by using 10 ppm hydrogen peroxide for 1 min.
The Egyptian essential oils derived from the fresh green parts of clove (Syzygium aromaticum), eu... more The Egyptian essential oils derived from the fresh green parts of clove (Syzygium aromaticum), eucalyptus (Cinnamomum eucalyptusa), lemongrass (Cymbopogon citratus), and sweet basil (Osmium basilicum) were evaluated under greenhouse conditions for their oviposition deterrent activity against Rhynchophorus ferrugineus. The highest oviposition deterrent activity was shown by S. aromaticum followed by C. eucalyptusa and cv. citratus oils (at 15% concentration) with values of 98.17%, 97.9% and 94.06 effective repellency, respectively. Cloves and eucalyptus essential oils at concentration of 10 %, significantly reduced egg laying and gave good practical oviposition deterrent effect (80.74 and 66.77% repellency). Moreover, a mixture of S. aromaticum and C. eucalyptusa oils (at 15% concentration) exhibited the higher oviposition deterrent activity (100%) than the other tested oils or their mixtures. These results clearly revealed that both essential oils (S. aromaticum and C. eucalyptusa) can be included as an integral parts of an IPM program against the date palm weevil.
In the present investigation, date palm plant regeneration procedure overcoming four different fu... more In the present investigation, date palm plant regeneration procedure overcoming four different fungus contamination using biological control was described. Three different concentration treatments of actinomycetes culture filtrate (5, 10 and 15%) from two isolates (Streptomyces bobilii and Streptomyces grisiobrunneus) were used to study their effect in overcoming four different fungi which contaminate in vitro date palm i.e. Fusarium moniliforme, Rhizoctonia solani, Macrophomina phaseolina and Alternaria alternata. Medium supplemented with S. bobilii surpassed medium supplemented with S. grisiobrunneus in regeneration characteristic values. Actinomycetes 10 % treatment proved to be the best for enhancing both survived explant and callus induction percentage, while 15% treatment proved to be the best for enhancing regenerated shoots per callus. Herein, we report improved regeneration system for Khalas cultivar, which is strongly needed for successful genetic transformation, overcoming fungi contamination destructiveness.
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