Ghada A. Ali

Many embryogenic systems have been designed to generate somatic embryos (SEs) with the morphology, biochemistry, and vigor uniformity of zygotic embryos (ZEs). During the current investigation, several antioxidants were added to the maturation media of the developing somatic embryos of date palm. Explant material was a friable embryogenic callus that was placed in maturation media containing ABA at 0.5 mg L−1, 5 g L−1 polyethylene glycol, and 10 g L−1 phytagel. Furthermore, α-tocopherol or reduced glutathione (GSH) were used separately at (25 and 50 mg L−1). These treatments were compared to a widely used date palm combination of reduced ascorbic acid (ASC) and citric acid at 150 and 100 mg L−1, respectively, and to the medium free from any antioxidants. The relative growth percentage of embryogenic callus (EC), globularization degree, differentiation%, and SEs number were significantly increased with GSH (50 mg L−1). Additionally, the latter treatment significantly enhanced the con...

Nowadays, nanotechnology has enriched all life sciences. However, regarding plant tissue culture, phyto nanotechnology was used to increase propagation, biomass production and plant protection. In this response, the effect of iron nanoparticles (Fe NPs) was investigated when added into the In vitro proliferation medium of date palm cv. Sewi somatic embryos with five different concentrations compared to a control. Results indicated a relatively positive impact of Fe NPs on the number of embryos and leaves when added with different doses to the culture medium. Added Fe NPs at concentrations 0.1, 1.0 and 2.0 ml/l resulted in the highest number of leaves 48.20, 52.00 and 46.33 leaf/explant, respectively with leaves’ elongation and growth improvement of shoots compared to the control. However, the highest number of somatic embryos 30.33 and 23.67 embryos/explant was recorded with using 0.5 ml/l Fe NPs in the two subcultures. Adding 1.0 ml/l Fe NPs to the culture medium resulted in maximu...

Food safety problems caused by pesticide residues especially in date palm fruits have become one of the most important issues that threaten human health regarding that some farmers are not looking to the optimal use of these pesticides. In the present study, Evaluation of initial residues of different pesticides (Chlorpyrifos, Fipronil, Methomyl, and Carbendazim) varied from (0.34-3.11 mg/kg) above MRLs reflecting improper use. In order to deal that, recent technologies where utilized to reduce residues. Barhee cv date palm samples were subjected to two recent decontamination methods (Ozone and60CO γ-Radiation). The remaining residues were analyzed by GC-MS/MS and LC-MS/MS using validated QuEChERS methodology. The results showed that removal of pesticides (89.6%-97.3%) using 1.0 KGy60CO, γ-Radiation was effectively reduced chlorpyrifos residues to lower than MRLs while ozone method at 60 min reduced carbendazim residues (89.7%-96.8%) below its MRLs. Both technologies were able to de...

This investigation was carried out during two successive experiments (2013 and 2014) at the tissue culture laboratory, Horticulture Research Institute, Giza, Egypt. The aim of this study was to induce variations in Red Roomy grape vine cultivar through in vitro mutagenesis by treating the axillary buds with two mutagenic, i.e. gamma rays at doses 0, 2, 4 and 6 Krad and varying concentrations of sodium azide 0, 0.01, 0.02 and 0.03 mg/L. The study applied ISSR analysis for discovery of genetic polymorphism among the variants and detect molecular markers associated with mutations and genetic variations. The results of analysis of variance indicated that the studied characters (explant height, shoot thickness, No. of leaves, root length, root thickness, No. of roots, survived explants and acclimized plants) were influenced by both gamma rays and sodium azide doses and gave significant differences caused by different treatments. However, mutagenic treatments with 2 or 4 Krad gamma rays a...