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Background: Neonatal thrombocytopenia is defined as a platelet count <150,000/μL can be mild, moderate, or severe thrombocytopenia. The platelet production in neonates begins early at 5 weeks of gestation and reaches the adults count... more
Background: Neonatal thrombocytopenia is defined as a platelet count <150,000/μL can be mild, moderate, or severe thrombocytopenia. The platelet production in neonates begins early at 5 weeks of gestation and reaches the adults count by 24–28 weeks. The platelet disorders are due to impaired platelet production or increased platelet destruction and sequestration. The onset of neonatal thrombocytopenia occurs within 72 h and late onset develops the risk of intraventricular hemorrhage. Hence, the knowledge regarding the incidence and occurrence is vital. Aims and Objectives: The present study aims to study the prevalence of neonatal thrombocytopenia in India. Materials and Methods: A cross-sectional descriptive study was designed, A total of 3250 neonates were assessed for the prevalence of neonatal thrombocytopenia using non-probability convenient sampling technique studies at various cities such as Lucknow, Panipat, Sonipat, Gohana, and Delhi. The data relating to levels of plate...
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Pseudothrombocytopenia is a complex phenomenon common nding in clinical laboratory error, misdiagnosis, inappropriate analysis caused due to various factors like time, temperature, technique, anticoagulants used for collecting samples,... more
Pseudothrombocytopenia is a complex phenomenon common nding in clinical laboratory error, misdiagnosis, inappropriate analysis caused due to various factors like time, temperature, technique, anticoagulants used for collecting samples, and few disease conditions the underlying mechanism is autoantibodies forming the clumping of the platelets which is interpretive as low platelet count, the present article discuss on various factors, mechanism and measures to correct the error and management of the pseudothrombocytopenia.
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Objective: To examine the distribution, emergence, and spread of genes encoding beta-lactamase resistance in Citrobacter species isolated from hospitalized patients in a tertiary care hospital. Methods: A prospective study was conducted... more
Objective: To examine the distribution, emergence, and spread of genes encoding beta-lactamase resistance in Citrobacter species isolated from hospitalized patients in a tertiary care hospital. Methods: A prospective study was conducted in a 1000-bed tertiary care center in Pune, India from October 2010 to October 2013. A total of 221 Citrobacter spp. isolates were recovered from clinical specimens from different patients (one isolate per patient) admitted to the surgical ward, medical ward and medical and surgical Intensive Care Units. Polymerase chain reaction (PCR) assays and sequencing were used to determine the presence of beta-lactamase encoding genes. Conjugation experiments were performed to determine their transferability. Isolate relatedness were determined by repetitive element based-PCR, enterobacterial repetitive intergenic consensus-PCR and randomly amplified polymorphic DNA. Results: Among 221 tested isolates of Citrobacter spp. recovered from various clinical specime...
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Objective. To detect genes encoding carbapenem resistance among Enterobacter species in a tertiary care hospital in central India. Methods. Bacterial identification of Enterobacter spp. isolates from various clinical specimens in patients... more
Objective. To detect genes encoding carbapenem resistance among Enterobacter species in a tertiary care hospital in central India. Methods. Bacterial identification of Enterobacter spp. isolates from various clinical specimens in patients admitted to intensive care units was performed by routine conventional microbial culture and biochemical tests using standard recommended techniques. Antibiotic sensitivity test was performed by standard Kirby Bauer disc diffusion technique. PCR amplification and automated sequencing was carried out. Transfer of resistance genes was determined by conjugation. Results. A total of 70/130 (53.84%) isolates of Enterobacter spp. were found to exhibit reduced susceptibility to imipenem (diameter of zones of inhibition ≤13 mm) by disc diffusion method. Among 70 isolates tested, 48 (68.57%) isolates showed MIC values for imipenem and meropenem ranging from 32 to 64 mg/L as per CLSI breakpoints. All of these 70 isolates were found susceptible to colistin in...
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To detect genes encoding carbapenem resistance in urinary isolates of Escherichia coli recovered from hospitalized patients in tertiary care centre in Pune, India. From Jan 2012 to Dec 2012, a total of 300 consecutive non-duplicate (one... more
To detect genes encoding carbapenem resistance in urinary isolates of Escherichia coli recovered from hospitalized patients in tertiary care centre in Pune, India. From Jan 2012 to Dec 2012, a total of 300 consecutive non-duplicate (one isolate per patient) clinical isolates of Escherichia coli were recovered from urine cultures of hospitalized patients including hospital acquired infection cases admitted to the medical and surgical intensive care units. Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of beta-lactamase encoding genes. Conjugation experiments were performed to determine the transferability of beta-lactamase. All the isolates were completely resistant to the second and third generation cephalosporins tested as well as carbapenems. All the isolates showed 100% susceptibility to tigecycline and colistin in vitro. Conjugation experiments demonstrated that blaNDM-1 was transferable via plasmid. All the isolates showed presence of b...
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Research Interests: Microbiology, Medicine, Colistin, Imipenem, Tigecycline, and 4 moreTicarcillin, meropenem, Piperacillin, and Cefepime
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The objective of the following study is to detect genes encoding carbapenem resistance in Klebsiella pneumoniae sepsis outbreak in a neonatal intensive care unit (NICU). Antibiotic sensitivity test was performed by standard Kirby Bauer... more
The objective of the following study is to detect genes encoding carbapenem resistance in Klebsiella pneumoniae sepsis outbreak in a neonatal intensive care unit (NICU). Antibiotic sensitivity test was performed by standard Kirby Bauer disc diffusion technique and minimum inhibitory concentrations of antibiotics was determined by VITEK-2. Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of beta-lactamase encoding genes. Conjugation experiments were performed to determine the transferability of beta-lactamase. Isolate relatedness were determined by repetitive-element PCR (REP), enterobacterial repetitive intergenic consensus (ERIC) PCR and random amplified polymorphic deoxyribonucleic acid (RAPD). All the isolates were completely resistant to the second and third generation cephalosporins tested as well as carbapenems. Susceptibility profiling of the isolates indicated that 100% retained susceptibility to tigecycline and colistin. Conjugation experiments indicated that blaNDM-1 was transferable and likely through a plasmid-mediated event. All the isolates showed the presence of blaNDM-1 with co association of blaCTX-M-15. REP-PCR, ERIC-PCR and RAPD revealed a single clonal type circulating in NICU environment. Co-production of NDM-1 with CTX-M-15 in K. pneumoniae isolates was detected for the first time in our NICU. Transmission of plasmid carrying these resistant genes to other members of Enterobacteriaceae will increase the incidence of multidrug resistance. Early detection of these genes will help in prevention and adequate infection control by limiting the spread of these organisms.
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A multidrug-resistant clinical isolate of Ralstonia pickettii from a woman was analysed. Modified Hodge test was positive for carbapenemase production. Conjugation experiment revealed the presence of conjugative plasmid of... more
A multidrug-resistant clinical isolate of Ralstonia pickettii from a woman was analysed. Modified Hodge test was positive for carbapenemase production. Conjugation experiment revealed the presence of conjugative plasmid of &amp;amp;gt;140 Kb size typed as IncN type. This is the first report of emergence blaVIM-2 in R. pickettii in India.
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Thrombotic thrombocytopenic purpura is a microangiopathic hemolytic amenia characterized by fever, neurological dysfunction, renal dysfunction, thrombocytopenia, hemolytic anemia. TTP can be inherited or acquired, caused due to ADAMTS13... more
Thrombotic thrombocytopenic purpura is a microangiopathic hemolytic amenia characterized by fever, neurological dysfunction, renal dysfunction, thrombocytopenia, hemolytic anemia. TTP can be inherited or acquired, caused due to ADAMTS13 enzyme malfunctioning. As the condition is medical emergency timely treatment is essential and vital plasma exchange and chemotherapy are used to control the activities of the enzymes. The present article describes the causes, risk factors, diagnosis and standard treatment for the management of TTP.
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This study showed that the prevalence of 3GC resistant Enterobacteraceae and non‐fermenting Gram‐negative Bacilli in patients admitted to ICUs of our hospital was substantial while the prevalence of NDM‐1 was substantially low (3.3%). GI... more
This study showed that the prevalence of 3GC resistant Enterobacteraceae and non‐fermenting Gram‐negative Bacilli in patients admitted to ICUs of our hospital was substantial while the prevalence of NDM‐1 was substantially low (3.3%). GI colonisation by 3GC and carbapenem‐resistant pathogens increases the risk of clinical infections by these pathogens and subsequent human to human transmission.[3]