Jeanne Pawitan

Transplantation of bone marrow derived stem cells (BMSCs) has been reported inhibits liver fibrosis. Several in vitro studies by co-culturing BMSCs and hepatic stellate cells (HSCs) indirectly or directly in 2D models showed inhibition of HSC as the key player in liver fibrosis. In this study, we investigated direct effect of BMSCs on HSCs by co-culturing BMSCs and HSCs in 3D model as it represents the liver microenvironment with intricate cell-cell and cell-matrix interactions. Primary isolated rat HSCs and BMSCs were directly co-cultured at 1:1 ratio with hanging drop method. The monoculture of rat HSCs served as positive control. Mono-culture and co-culture samples were harvested on day 3, 5 and 7 for histological analysis. The samples were analyzed for extracellular matrix deposition by Masson's Trichrome staining, tenascin-C immunocytochemistry, resting HSC's state as shown by positive Oil Red O stained cells. Our results indicated CD90(＋)CD34(-) BMSCs anti-liver fibros...

Non-union due to large bone loss often causes significant long-term morbidity. We incorporate the use of allogeneic umbilical cord-derived mesenchymal stem cells (UC-MSCs) as part of the diamond concept of regenerative medicine in a case of infected non-union fracture. We reported a 54-year-old female patient presenting with pain on the right thigh. She was previously diagnosed with a closed fracture of the right femoral shaft and underwent four surgeries before finally being referred to Dr. Cipto Mangunkusumo General Hospital with infected non-union of the right femoral shaft. The patient was treated with a combination of UC-MSCs, bone morphogenetic protein-2 (BMP-2), Hydroxyapatite (HA), and mechanical stabilization using Masquelet Technique. The combination of allogeneic MSCs, BMP2, HA, and Masquelet Technique was successful in creating new bone with no apparent side effects. Bone loss might be caused by external factors (true defects), or structural loss of the existing bone. The combination of allogeneic UC-MSCs, BMP-2, HA and an induced membrane technique pioneered by Masquelet allowed for faster regeneration process and more optimal bone healing. This paper aims to assess and compare the result of such procedures with the previous four surgeries done to the patient, which did not yield satisfactory results. The application of allogeneic UC-MSC, BMP-2, HA and Masquelet technique as proposed in the diamond concept is a viable method in treating critical-sized bone defect and provides an effective way to overcome non-union caused by large defect.

Abstract Sciatic function index (SFI) has long been used as one of the evaluation methods of walking analysis. SFI is a method in assessing nerve function through footprints in walking analysis. Methods that previously have been developed use one color ink, but this method is difficult to interpret. The interpretation is difficult because of mingled footprints among the mice four legs. If rats do not walk straight, their footprints will be overlapped to one another and complicate the measurement. SFI is a method using a special mathematic formula. Many researchers questioning this method because this method is affected by the muscle contracture, auto mutilation and the way and speed of the rat walk. In this study we use four different colors, which allow us to more easily interpret the results of the footprint. In this study, we modify a tool from the existing tools in other literatures. The measurement result of this tool is print length (PL), inter toe spread (ITS) and toe spread ...

Platelet concentrate (PC) has been used as substitute to the use of fetal bovine serum (FBS) in cell culture media. However, it's use as additive in cell culture media showed inconsistent results on cell proliferation, and the inconsistent results may be due to variability of platelet counts or growth factor content. Standard protocols are lacking for the preparation of PC before it is applied as additive in cell culture media. The growth factor content of PC can be released by freeze-thaw cycles, which range from one to three cycles before use for cell culture media. This study aimed to measure base-line platelet counts and growth factor levels and compare to platelet counts and growth factor levels after one, two and three freeze-thaw cycles. In this study, we obtained PC from Indonesian Red Cross. The PCs were aliquoted and stored at-20 o C and then subjected to 1 to 3 freeze-thaw cycles. The number of platelet before and after freeze-thaw cycles were measured using Sysmex XN...

To compare the growth factor contents of the various PRP processing methods to get an insight on the difference in growth factor yield from the various processing methods. Experimental: PRP before and after expiry dates was collected from Indonesian Red Cross. PRP was processed by various methods to release the growth factors, and growth factor levels were measured. Growth factor levels between various kinds of processing were compared usingANOVAand those frombefore and after expiry date samples were compared using t test. Results: Comparison of thrombin activation, one, two, and three freeze thawcycle(s) of all samples revealed no significantdifference inPDGF-AB,EGF, and IGFlevels (p>0.05).However, there were significant differences in TGFâ and VEGF levels between thrombin activation and freeze thawcycles. Comparisons between samples of before and after expiry date revealed no significant differences in all growth factor levels. Conclusion: One, two, and three freeze thawcycle(s...

Acute promyelocytic leukemia (APL) adalah suatu subtipe acute myeloid leukemia yang berbeda . engan ciri akumulasi promyelosit abnormal pada sumsum tulang. Hasil pengobatan APL telah meningkat secara dramatis sehingga APL dapat dikatakan sebagai penyakit yang dapat disembuhkan. APL menunjukkan si satu aiel gena retinoic acid receptor-alpha (RAR) pada kromosom 17q21 dengan salah satu gen partner: \11-,PLZF,NPM,NuMA,danSTAT5b. StrategipenatalaksanaanAPLadalahinduksidifferensiasi,induksi ptosis terhadap sel dengan sitodifferensiasi yang tidak sempurna, dan menghambat aktivitas Histone zeacetylase (HDAC).

Background: Lipoaspirate contains noxious substances derived from liposuction. Therefore, extensive washing is recommended before the lipoaspirate is processed further for culture or fat grafting. Washing a small amount of lipoaspirate may not pose a problem, but washing a large volume of lipoaspirate may be cumbersome, time consuming, and requires a lot of phosphate buffered saline (PBS). Objective: To introduce a simple method for lipoaspirate washing using fine-mesh stainless-steel tea or coffee filter, a small tea spoon, and a porcelain bowl. Methods: The filter was used to collect the adipose tissue fragments. Further washing of the fragments was achieved by soaking the adipose tissue containing filter in a PBS containing porcelain bowl and stirring using a small tea spoon to transfer the contaminating materials to the PBS. Enzymatic processing to dissociate the cells from the tissue and primary cultures was conducted as usual in MesenCult. Results: Using the equipment mentione...

To obtain the normal value of micronuclei in peripheral blood mononuclear cells. We screened 300 blood samples for micronucleated cells. Samples from donors who smoked, were ill or lived in a polluted area were excluded. From each sample, 500 swollen mononuclear leukocytes were screened with a light microscope, using 400x magnification. A frequency distribution of micronucleated cell number was made, and the mean of micronucleated cell number was calculated. Further, the data were tested for the type of probability distribution using the test of goodness of fit, and the parameters were estimated. Of the 300 samples, 203 were excluded and 97 analyzed. In the 97 samples, the mean of micronucleated cells (from 500 cells screened) was 0.59; the data followed a Poisson distribution. The 95% confidence limits were .45 and .76. The normal value in unexposed individuals is .59 micronucleated cells per 500 cells.

Aims:  To address the possible use of RNA interference in diagnosis, prognosis and therapy of various diseases and explain the obstacles in RNA interference based therapy.Methods:  Review of the literature.Results and discussion:  The MicroRNAs (miRNAs) were shown to play a role in various normal and pathological biological processes, i.e. the regulation of cell cycle and apoptosis, cell differentiation, wound healing process, immune system, etc. Furthermore, abnormal expression of miRNA was found in various diseases. Therefore, when the sample can be easily collected, miRNA expression profile can be used to detect diseases where early diagnosis is advantageous, such as in various malignancies or diseases that show myriads of symptoms such as autoimmune diseases. Further, different expression of miRNA in tumour subtypes can be used to predict the subtypes and hence the prognosis. For therapy, small (short) interfering RNAs (siRNAs) can be developed to ‘switch off’ up-regulated genes or miRNAs or the suppressors of down-regulated genes or miRNAs. These approaches in various animal models of diseases showed promising results and human trials for viral infections are underway. However, obstacles to the application in human might be encountered, such as degradation of the siRNAs before it can exert its function, target cell penetration and ‘off target’ toxic effects. Still, it is believed that modification of the RNA, development of carrier vehicles and mode and route of administration might solve these obstacles.Conclusion:  MicroRNAs profile differences between normal and pathological condition might be promising as biomarker in early diagnosis and prognosis, while siRNA showed promising result in the therapy of various diseases in animal models.